1CLD: DNA-binding protein

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
PDB ID: 1CLDDownload
MMDB ID: 71748
PDB Deposition Date: 1995/6/6
Updated in MMDB: 2011/06
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 1CLD
Label Count Molecule
Protein (1 molecule)
Cd2-lac9(Gene symbol: KLLA0_D12672g)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB