1CGP: CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Citation:
Abstract
The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.
PDB ID: 1CGPDownload
MMDB ID: 71735
PDB Deposition Date: 1991/8/12
Updated in MMDB: 2018/06
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1CGP: hexameric; determined by author
Molecular Components in 1CGP
Label Count Molecule
Proteins (2 molecules)
2
Protein (Catabolite Gene Activator Protein (Cap))(Gene symbol: crp)
Molecule annotation
Nucleotides(2 molecules)
2
DNA (5'-d(*gp*cp*gp*ap*ap*ap*ap*gp*tp*gp*tp*gp*ap*cp*ap*tp*ap*t)-3')
Molecule annotation
2
DNA (5'-d(*gp*tp*cp*ap*cp*ap*cp*tp*tp*tp*tp*cp*g)-3')
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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