1BS1: Dethiobiotin Synthetase Complexed With Dethiobiotin, Adp , Inorganic Phosphate And Magnesium

Citation:
Abstract
The crystal structures of two complexes of dethiobiotin synthetase, enzyme-diaminopelargonic acid-MgADP-AlF3 and enzyme-dethiobiotin-MgADP-Pi, respectively, have been determined to 1.8 A resolution. In dethiobiotin synthetase, AlF3 together with carbamylated diaminopelargonic acid mimics the phosphorylated reaction intermediate rather than the transition state complex for phosphoryl transfer. Observed differences in the binding of substrate, diaminopelargonic acid, and the product, dethiobiotin, suggest considerable displacements of substrate atoms during the ring closure step of the catalytic reaction. In both complexes, two metal ions are observed at the active site, providing evidence for a two-metal mechanism for this enzyme.
PDB ID: 1BS1Download
MMDB ID: 11966
PDB Deposition Date: 1998/8/31
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 1BS1: monomeric; determined by author
Molecular Components in 1BS1
Label Count Molecule
Protein (1 molecule)
1
Protein (Dethiobiotin Synthetase)(Gene symbol: bioD)
Molecule annotation
Chemicals (4 molecules)
1
2
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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