1BP7: Group I Mobile Intron Endonuclease I-crei Complexed With Homing Site DNA

The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
PDB ID: 1BP7Download
MMDB ID: 71666
PDB Deposition Date: 1998/8/13
Updated in MMDB: 2011/06 
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1BP7: tetrameric; determined by author
Molecular Components in 1BP7
Label Count Molecule
Proteins (2 molecules)
Protein (I-crei)
Molecule annotation
Nucleotides(2 molecules)
DNA (5'- D(*gp*cp*ap*ap*ap*ap*cp*gp*tp*cp*gp*tp*gp*ap*gp*ap*cp*ap*gp *tp*tp*tp* Cp*g)-3')
Molecule annotation
DNA (5'- D(*cp*gp*ap*ap*ap*cp*tp*gp*tp*cp*tp*cp*ap*cp*gp*ap*cp*gp*tp *tp*tp*tp* Gp*c)-3')
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB