1AZP: HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX

Citation:
Abstract
The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution.
PDB ID: 1AZPDownload
MMDB ID: 71604
PDB Deposition Date: 1997/11/19
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 1.6  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1AZP: trimeric; determined by author
Molecular Components in 1AZP
Label Count Molecule
Protein (1 molecule)
1
Protein (Hyperthermophile Chromosomal Protein Sac7d)(Gene symbol: SACI_RS00290)
Molecule annotation
Nucleotide(1 molecule)
2
DNA (5'-d(*gp*cp*gp*ap*tp*cp*gp*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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