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Conserved domains on  [gi|1039748751|ref|XP_017172071|]
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rap guanine nucleotide exchange factor 3 isoform X3 [Mus musculus]

Protein Classification

DEP_Epac and RasGEF domain-containing protein( domain architecture ID 10858388)

protein containing domains DEP_Epac, CAP_ED, REM, and RasGEF

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
692-960 2.29e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


:

Pssm-ID: 214539  Cd Length: 242  Bit Score: 266.03  E-value: 2.29e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  692 LDLVSAKDLAGQLTDHDWNLFNRIHqvqEVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQL 771
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKID---PSELLGSVWGKRSKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAEL 77

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  772 LRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKL-SPPVIP 850
Cdd:smart00147  78 LSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIP 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  851 FMPLLLKDVTFIHEGNHTLVE-NLINFEKMRMMARAVRMLHHCRShSTAPLSPLRsrvSHIHEDSQGSRIstcseqslst 929
Cdd:smart00147 158 FLGVLLKDLTFIDEGNPDFLEnGLVNFEKRRQIAEILREIRQLQS-QPYNLRPNR---SDIQSLLQQLLD---------- 223

                          250       260       270
                   ....*....|....*....|....*....|.
gi 1039748751  930 rspastwayvqqlkVIDNQRELSRLSRELEP 960
Cdd:smart00147 224 --------------HLDEEEELYQLSLKIEP 240
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 131-254 2.53e-56

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


:

Pssm-ID: 239884  Cd Length: 125  Bit Score: 190.25  E-value: 2.53e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 131 AGKLLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDa 210
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKY- 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1039748751 211 QFYRFPGPEPEPTGTQ--DVEEELVEAMALLSQRGPDALLTVALRK 254
Cdd:cd04437    80 QFYRFSDDECSPAPLEkrEAEEELQEAVTLLSQLGPDALLRMILRK 125
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 423-544 5.52e-19

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


:

Pssm-ID: 100121  Cd Length: 122  Bit Score: 83.62  E-value: 5.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 423 TPEKILELLLEAMRPDSSahdpteTFLSDFLLTHSVFMPSTQLFTALLHHFHVEPaDPAGGSEQEHSTYICNKRQQILRL 502
Cdd:cd06224     1 TLEALIEHLTSTFDMPDP------SFVSTFLLTYRSFTTPTELLEKLIERYEIAP-PENLEYNDWDKKKSKPIRLRVLNV 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1039748751 503 VGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQ 544
Cdd:cd06224    74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKL 115
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 278-387 4.81e-18

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


:

Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 80.83  E-value: 4.81e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 278 AHLSNSVKRELAAVLlFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPRA 353
Cdd:cd00038     3 SGLDDEELEELADAL-EERRFPAGEVIIRQGDPADSLYIVLSGSVEVYKLDEDgreqIVGFLGPGDLFGELALLGNGPRS 81

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1039748751 354 ATIILREnNCHFLRVDKQDFNRIIKDVEAKTMRL 387
Cdd:cd00038    82 ATVRALT-DSELLVLPRSDFRRLLQEYPELARRL 114
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
692-960 2.29e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 266.03  E-value: 2.29e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  692 LDLVSAKDLAGQLTDHDWNLFNRIHqvqEVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQL 771
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKID---PSELLGSVWGKRSKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAEL 77

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  772 LRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKL-SPPVIP 850
Cdd:smart00147  78 LSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIP 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  851 FMPLLLKDVTFIHEGNHTLVE-NLINFEKMRMMARAVRMLHHCRShSTAPLSPLRsrvSHIHEDSQGSRIstcseqslst 929
Cdd:smart00147 158 FLGVLLKDLTFIDEGNPDFLEnGLVNFEKRRQIAEILREIRQLQS-QPYNLRPNR---SDIQSLLQQLLD---------- 223

                          250       260       270
                   ....*....|....*....|....*....|.
gi 1039748751  930 rspastwayvqqlkVIDNQRELSRLSRELEP 960
Cdd:smart00147 224 --------------HLDEEEELYQLSLKIEP 240
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 692-927 6.52e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 261.80  E-value: 6.52e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 692 LDLVSAKDLAGQLTDHDWNLFNRIHqvqEVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQL 771
Cdd:cd00155     1 FLSLDPKELAEQLTLLDFELFRKIE---PFELLGSLWSKKDKNIHLSPNLERFIERFNNLSNWVASEILLCTNPKKRARL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 772 LRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKL--SPPVI 849
Cdd:cd00155    78 LSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVgpNPPCV 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 850 PFMPLLLKDVTFIHEGNHTLVE-NLINFEKMRMMARAVRMLHHCRS--HSTAPLSPLRSRVSHIHEDSQgsRISTCSEQS 926
Cdd:cd00155   158 PFLGVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSnsYELNRDEDILAFLWKLLELIL--NEDELYELS 235


                  .
gi 1039748751 927 L 927
Cdd:cd00155   236 L 236
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 699-880 1.79e-67

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 223.24  E-value: 1.79e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 699 DLAGQLTDHDWNLFNRIHqvqEVELIHYVLGpQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQLLRKFIKL 778
Cdd:pfam00617   1 ELARQLTLIEFELFRKIK---PRELLGSAWS-KKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 779 AAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKD 858
Cdd:pfam00617  77 AEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTD 156

                         170       180
                  ....*....|....*....|...
gi 1039748751 859 VTFIHEGNHTLVEN-LINFEKMR 880
Cdd:pfam00617 157 LTFIEEGNPDFLEGgLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 131-254 2.53e-56

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 190.25  E-value: 2.53e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 131 AGKLLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDa 210
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKY- 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1039748751 211 QFYRFPGPEPEPTGTQ--DVEEELVEAMALLSQRGPDALLTVALRK 254
Cdd:cd04437    80 QFYRFSDDECSPAPLEkrEAEEELQEAVTLLSQLGPDALLRMILRK 125
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 423-544 5.52e-19

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 83.62  E-value: 5.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 423 TPEKILELLLEAMRPDSSahdpteTFLSDFLLTHSVFMPSTQLFTALLHHFHVEPaDPAGGSEQEHSTYICNKRQQILRL 502
Cdd:cd06224     1 TLEALIEHLTSTFDMPDP------SFVSTFLLTYRSFTTPTELLEKLIERYEIAP-PENLEYNDWDKKKSKPIRLRVLNV 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1039748751 503 VGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQ 544
Cdd:cd06224    74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKL 115
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 418-527 6.72e-19

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 82.74  E-value: 6.72e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 418 TVMSGTPEKILELLLEAMRpdssahDPTETFLSDFLLTHSVFMPSTQLFTALLHHFHVEPADPAGGSEQEHSTYICNKRQ 497
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRI------MLDDSFLSTFLLTYRSFTTPAELLELLIERYNIPPPLDLSSDSYWISKKTLPIRI 74

                          90       100       110
                  ....*....|....*....|....*....|
gi 1039748751 498 QILRLVGRWVALYSPMLHSDPVATSFLQKL 527
Cdd:pfam00618  75 RVLSVLRHWVENYFSDFNDDPVLLSRLEKF 104
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 142-217 1.70e-18

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 80.79  E-value: 1.70e-18
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1039748751  142 TYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLgVHSRSQAVGICQVLLDEGALCHV--KHDWTFQDrDAQFYRFPG 217
Cdd:smart00049   2 ETGLKLRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVngPNKHTFKD-SKALYRFTT 77
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 278-387 4.81e-18

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 80.83  E-value: 4.81e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 278 AHLSNSVKRELAAVLlFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPRA 353
Cdd:cd00038     3 SGLDDEELEELADAL-EERRFPAGEVIIRQGDPADSLYIVLSGSVEVYKLDEDgreqIVGFLGPGDLFGELALLGNGPRS 81

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1039748751 354 ATIILREnNCHFLRVDKQDFNRIIKDVEAKTMRL 387
Cdd:cd00038    82 ATVRALT-DSELLVLPRSDFRRLLQEYPELARRL 114
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 299-379 1.50e-16

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 75.34  E-value: 1.50e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 299 KAGTVLFSQGDKGTSWYIIWKGSVNVVTHG----KGLVTTLHEGDDFGQLALVNDAPRAATIILREnNCHFLRVDKQDFN 374
Cdd:pfam00027   5 KAGEVIFREGDPADSLYIVLSGKVKVYRTLedgrEQILAVLGPGDFFGELALLGGEPRSATVVALT-DSELLVIPREDFL 83


                  ....*
gi 1039748751 375 RIIKD 379
Cdd:pfam00027  84 ELLER 88
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 415-542 6.19e-16

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 75.06  E-value: 6.19e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  415 NRYTVMSGTPEKILELLLEAMrpdssaHDPTETFLSDFLLTHSVFMPSTQLFTALLHHFhvEPADPAGGSEQEHSTYICN 494
Cdd:smart00229   1 DGGLIKGGTLEALIEHLTEAF------DKADPSFVETFLLTYRSFITTQELLQLLLYRY--NAIPPESWVEEKVNPRRVK 72

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1039748751  495 KRqqILRLVGRWVALYSPMLHSDPVATSFLQKLSDLVSRDA------RLSNLLR 542
Cdd:smart00229  73 NR--VLNILRTWVENYWEDFEDDPKLISFLLEFLELVDDEKypglvtSLLNLLR 124
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 278-390 1.21e-14

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 71.28  E-value: 1.21e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  278 AHLSNSVKRELAAVLlfEPHS-KAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPR 352
Cdd:smart00100   3 KNLDAEELRELADAL--EPVRyPAGEVIIRQGDVGDSFYIIVSGEVEVYKVLEDgeeqIVGTLGPGDFFGELALLTNSRR 80

                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 1039748751  353 AATIILREnnCHFLRVDKQDFNRIIKDVEAKTMRLEEH 390
Cdd:smart00100  81 AASAAAVA--LELATLLRIDFRDFLQLLPELPQLLLEL 116
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 147-215 1.28e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 69.54  E-value: 1.28e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 147 IRDRKYHLRLYRHCCSGRELVDGILALGLgVHSRSQAVGICQVLLDEGALCHV-KHDWTFQDrDAQFYRF 215
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKD-SYYFYRF 71
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 278-379 7.73e-09

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 56.92  E-value: 7.73e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 278 AHLSNSVKRELAAvlLFEPHS-KAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPR 352
Cdd:COG0664     2 AGLSDEELEALLA--HLELRTlKKGEVLFREGDPADHLYFVLSGLVKLYRISEDgreqILGFLGPGDFFGELSLLGGEPS 79

                          90       100
                  ....*....|....*....|....*..
gi 1039748751 353 AATIILREnNCHFLRVDKQDFNRIIKD 379
Cdd:COG0664    80 PATAEALE-DSELLRIPREDLEELLER 105
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
692-960 2.29e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 266.03  E-value: 2.29e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  692 LDLVSAKDLAGQLTDHDWNLFNRIHqvqEVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQL 771
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKID---PSELLGSVWGKRSKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAEL 77

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  772 LRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKL-SPPVIP 850
Cdd:smart00147  78 LSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIP 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  851 FMPLLLKDVTFIHEGNHTLVE-NLINFEKMRMMARAVRMLHHCRShSTAPLSPLRsrvSHIHEDSQGSRIstcseqslst 929
Cdd:smart00147 158 FLGVLLKDLTFIDEGNPDFLEnGLVNFEKRRQIAEILREIRQLQS-QPYNLRPNR---SDIQSLLQQLLD---------- 223

                          250       260       270
                   ....*....|....*....|....*....|.
gi 1039748751  930 rspastwayvqqlkVIDNQRELSRLSRELEP 960
Cdd:smart00147 224 --------------HLDEEEELYQLSLKIEP 240
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 692-927 6.52e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 261.80  E-value: 6.52e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 692 LDLVSAKDLAGQLTDHDWNLFNRIHqvqEVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQL 771
Cdd:cd00155     1 FLSLDPKELAEQLTLLDFELFRKIE---PFELLGSLWSKKDKNIHLSPNLERFIERFNNLSNWVASEILLCTNPKKRARL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 772 LRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKL--SPPVI 849
Cdd:cd00155    78 LSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVgpNPPCV 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 850 PFMPLLLKDVTFIHEGNHTLVE-NLINFEKMRMMARAVRMLHHCRS--HSTAPLSPLRSRVSHIHEDSQgsRISTCSEQS 926
Cdd:cd00155   158 PFLGVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSnsYELNRDEDILAFLWKLLELIL--NEDELYELS 235


                  .
gi 1039748751 927 L 927
Cdd:cd00155   236 L 236
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 699-880 1.79e-67

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 223.24  E-value: 1.79e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 699 DLAGQLTDHDWNLFNRIHqvqEVELIHYVLGpQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQLLRKFIKL 778
Cdd:pfam00617   1 ELARQLTLIEFELFRKIK---PRELLGSAWS-KKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 779 AAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKD 858
Cdd:pfam00617  77 AEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTD 156

                         170       180
                  ....*....|....*....|...
gi 1039748751 859 VTFIHEGNHTLVEN-LINFEKMR 880
Cdd:pfam00617 157 LTFIEEGNPDFLEGgLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 131-254 2.53e-56

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 190.25  E-value: 2.53e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 131 AGKLLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDa 210
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKY- 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1039748751 211 QFYRFPGPEPEPTGTQ--DVEEELVEAMALLSQRGPDALLTVALRK 254
Cdd:cd04437    80 QFYRFSDDECSPAPLEkrEAEEELQEAVTLLSQLGPDALLRMILRK 125
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 423-544 5.52e-19

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 83.62  E-value: 5.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 423 TPEKILELLLEAMRPDSSahdpteTFLSDFLLTHSVFMPSTQLFTALLHHFHVEPaDPAGGSEQEHSTYICNKRQQILRL 502
Cdd:cd06224     1 TLEALIEHLTSTFDMPDP------SFVSTFLLTYRSFTTPTELLEKLIERYEIAP-PENLEYNDWDKKKSKPIRLRVLNV 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1039748751 503 VGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQ 544
Cdd:cd06224    74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKL 115
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 418-527 6.72e-19

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 82.74  E-value: 6.72e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 418 TVMSGTPEKILELLLEAMRpdssahDPTETFLSDFLLTHSVFMPSTQLFTALLHHFHVEPADPAGGSEQEHSTYICNKRQ 497
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRI------MLDDSFLSTFLLTYRSFTTPAELLELLIERYNIPPPLDLSSDSYWISKKTLPIRI 74

                          90       100       110
                  ....*....|....*....|....*....|
gi 1039748751 498 QILRLVGRWVALYSPMLHSDPVATSFLQKL 527
Cdd:pfam00618  75 RVLSVLRHWVENYFSDFNDDPVLLSRLEKF 104
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 142-217 1.70e-18

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 80.79  E-value: 1.70e-18
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1039748751  142 TYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLgVHSRSQAVGICQVLLDEGALCHV--KHDWTFQDrDAQFYRFPG 217
Cdd:smart00049   2 ETGLKLRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVngPNKHTFKD-SKALYRFTT 77
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 278-387 4.81e-18

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 80.83  E-value: 4.81e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 278 AHLSNSVKRELAAVLlFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPRA 353
Cdd:cd00038     3 SGLDDEELEELADAL-EERRFPAGEVIIRQGDPADSLYIVLSGSVEVYKLDEDgreqIVGFLGPGDLFGELALLGNGPRS 81

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1039748751 354 ATIILREnNCHFLRVDKQDFNRIIKDVEAKTMRL 387
Cdd:cd00038    82 ATVRALT-DSELLVLPRSDFRRLLQEYPELARRL 114
DEP cd04371
DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first ...
 134-214 9.27e-17

DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first discovered. The function of this domain is still not clear, but it is believed to be important for the membrane association of the signaling proteins in which it is present. New studies show that the DEP domain of Sst2, a yeast RGS protein is necessary and sufficient for receptor interaction.


Pssm-ID: 239836  Cd Length: 81  Bit Score: 75.84  E-value: 9.27e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 134 LLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVhSRSQAVGICQVLLDEGALCHV-KHDWTFQDrDAQF 212
Cdd:cd04371     2 LVRIMLDSDSGVPIKDRKYHLKTYPNCFTGSELVDWLLDNLEAI-TREEAVELGQALLKHGLIHHVsDDKHTFRD-SYAL 79


                  ..
gi 1039748751 213 YR 214
Cdd:cd04371    80 YR 81
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 299-379 1.50e-16

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 75.34  E-value: 1.50e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 299 KAGTVLFSQGDKGTSWYIIWKGSVNVVTHG----KGLVTTLHEGDDFGQLALVNDAPRAATIILREnNCHFLRVDKQDFN 374
Cdd:pfam00027   5 KAGEVIFREGDPADSLYIVLSGKVKVYRTLedgrEQILAVLGPGDFFGELALLGGEPRSATVVALT-DSELLVIPREDFL 83


                  ....*
gi 1039748751 375 RIIKD 379
Cdd:pfam00027  84 ELLER 88
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 415-542 6.19e-16

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 75.06  E-value: 6.19e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  415 NRYTVMSGTPEKILELLLEAMrpdssaHDPTETFLSDFLLTHSVFMPSTQLFTALLHHFhvEPADPAGGSEQEHSTYICN 494
Cdd:smart00229   1 DGGLIKGGTLEALIEHLTEAF------DKADPSFVETFLLTYRSFITTQELLQLLLYRY--NAIPPESWVEEKVNPRRVK 72

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1039748751  495 KRqqILRLVGRWVALYSPMLHSDPVATSFLQKLSDLVSRDA------RLSNLLR 542
Cdd:smart00229  73 NR--VLNILRTWVENYWEDFEDDPKLISFLLEFLELVDDEKypglvtSLLNLLR 124
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 278-390 1.21e-14

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 71.28  E-value: 1.21e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751  278 AHLSNSVKRELAAVLlfEPHS-KAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPR 352
Cdd:smart00100   3 KNLDAEELRELADAL--EPVRyPAGEVIIRQGDVGDSFYIIVSGEVEVYKVLEDgeeqIVGTLGPGDFFGELALLTNSRR 80

                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 1039748751  353 AATIILREnnCHFLRVDKQDFNRIIKDVEAKTMRLEEH 390
Cdd:smart00100  81 AASAAAVA--LELATLLRIDFRDFLQLLPELPQLLLEL 116
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 147-215 1.28e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 69.54  E-value: 1.28e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 147 IRDRKYHLRLYRHCCSGRELVDGILALGLgVHSRSQAVGICQVLLDEGALCHV-KHDWTFQDrDAQFYRF 215
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKD-SYYFYRF 71
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 278-379 7.73e-09

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 56.92  E-value: 7.73e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 278 AHLSNSVKRELAAvlLFEPHS-KAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKG----LVTTLHEGDDFGQLALVNDAPR 352
Cdd:COG0664     2 AGLSDEELEALLA--HLELRTlKKGEVLFREGDPADHLYFVLSGLVKLYRISEDgreqILGFLGPGDFFGELSLLGGEPS 79

                          90       100
                  ....*....|....*....|....*..
gi 1039748751 353 AATIILREnNCHFLRVDKQDFNRIIKD 379
Cdd:COG0664    80 PATAEALE-DSELLRIPREDLEELLER 105
DEP_PIKfyve cd04448
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange ...
 146-212 2.94e-07

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange factor) PIKfyve-like proteins. PIKfyve contains N-terminal Fyve finger and DEP domains, a central chaperonin-like domain and a C-terminal PIPK (phosphatidylinositol phosphate kinase) domain. PIKfyve-like proteins are important phosphatidylinositol (3)-monophosphate (PtdIns(3)P)-5-kinases, producing PtdIns(3,5)P2, which plays a major role in multivesicular body (MVB) sorting and control of retrograde traffic from the vacuole back to the endosome and/or Golgi. PIKfyve itself has been shown to be play a role in regulating early-endosome-to-trans-Golgi network (TGN) retrograde trafficking.


Pssm-ID: 239895  Cd Length: 81  Bit Score: 48.98  E-value: 2.94e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1039748751 146 LIRDRKYHLRLYRHCCSGRELVDGILALGLgVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQF 212
Cdd:cd04448    14 EFQDHRYRLRTYTNCILGKELVNWLIRQGK-AATRVQAIAIGQALLDAGWIECVSDDDLFRDEYALY 79
DEP_1_DEP6 cd04442
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins ...
 146-215 1.14e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239889 [Multi-domain]  Cd Length: 82  Bit Score: 44.50  E-value: 1.14e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1039748751 146 LIRDRKYHLRLYRHCCSGRELVDGILAlglgvHS----RSQAVGICQVLLDEGALCHVkHDWTFQDRDAQ-FYRF 215
Cdd:cd04442    14 VIKDRRHHLRTYPNCFVGKELIDWLIE-----HKeasdRETAIKIMQKLLDHSIIHHV-CDEHKEFKDAKlFYRF 82
DEP_2_P-Rex cd04440
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in P-Rex-like proteins. The P-Rex ...
 126-215 3.42e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in P-Rex-like proteins. The P-Rex family is the guanine-nucleotide exchange factor (GEF) for the small GTPase Rac that contains an N-terminal RhoGEF domain, two DEP and PDZ domains. Rac-GEF activity is stimulated by phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), a lipid second messenger, and the G beta-gamma subunits of heterotrimeric G proteins. The DEP domains are not involved in mediating these stimuli, but may be of importance for basal and stimulated levels Rac-GEF activity.


Pssm-ID: 239887  Cd Length: 93  Bit Score: 43.37  E-value: 3.42e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 126 EHVHKAGKLLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGlGVHSRSQAVGICQVLLDEGALCHVKHDWTF 205
Cdd:cd04440     3 EDIMSKGVRLYCRLHSLFTPVVKDRDYHLKTYKSVVPASKLVDWLLAQG-DCRTREEAVILGVGLCNNGFMHHVLEKSEF 81

                          90
                  ....*....|
gi 1039748751 206 QDrDAQFYRF 215
Cdd:cd04440    82 KD-EPLLFRF 90
DEP_GPR155 cd04443
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like ...
 146-215 5.00e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like proteins, also known as PGR22, contain an N-terminal permease domain, a central transmembrane region and a C-terminal DEP domain. They are orphan receptors of the class B G protein-coupled receptors. Their function is unknown.


Pssm-ID: 239890 [Multi-domain]  Cd Length: 83  Bit Score: 42.70  E-value: 5.00e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 146 LIRDRKYHLRLYRHCCSGRELVDGILALGLGVhSRSQAVGICQVLLDEGALCHVKHDWTFQDrDAQFYRF 215
Cdd:cd04443    16 IVKDRRCGLRTYKGVFCGCDLVSWLIEVGLAQ-DRGEAVLYGRRLLQGGVLQHITNEHHFRD-ENLLYRF 83
DEP_DEPDC4 cd04446
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a ...
 147-214 8.06e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a DEP domain containing protein of unknown function.


Pssm-ID: 239893  Cd Length: 95  Bit Score: 42.35  E-value: 8.06e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 147 IRDRKYHLRLYRHCCSGRELVDGILA-------LGLGVHSRSQAVGICQVLLDegalCHV-----------KHDWTFQDR 208
Cdd:cd04446    14 VKKRRHNLKSYHDCFLGSEAVDVVLAhlmqnkyFGDVDVPRAKAVRLCQALMD----CRVfeavgtkvfkkKKRAVFEDS 89


                  ....*.
gi 1039748751 209 DAQFYR 214
Cdd:cd04446    90 SSSLYR 95
DEP_1_P-Rex cd04439
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in P-Rex-like proteins. The P-Rex ...
 132-215 2.68e-04

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in P-Rex-like proteins. The P-Rex family is the guanine-nucleotide exchange factor (GEF) for the small GTPase Rac that contains an N-terminal RhoGEF domain, two DEP and PDZ domains. Rac-GEF activity is stimulated by phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), a lipid second messenger, and by the G beta-gamma subunits of heterotrimeric G proteins. The DEP domains are not involved in mediating these stimuli, but may be of importance for basal and stimulated levels Rac-GEF activity.


Pssm-ID: 239886  Cd Length: 81  Bit Score: 40.63  E-value: 2.68e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 132 GKLLHrHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGlGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDrDAQ 211
Cdd:cd04439     1 GEKLY-KMMCKQGSLIKDRRRKLSTFPKCFLGNEFVSWLLEIG-EISKPEEGVNLGQALLENGIIHHVSDKHQFKN-EQV 77


                  ....
gi 1039748751 212 FYRF 215
Cdd:cd04439    78 LYRF 81
DEP_2_DEP6 cd04441
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins ...
 132-215 7.24e-04

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239888  Cd Length: 85  Bit Score: 39.34  E-value: 7.24e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039748751 132 GKLLHRHLLATYPTLIRDRKYHLRLYRHCCSGRELVDGILALGlGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAq 211
Cdd:cd04441     4 GQRLYEKLMSTENSILQVREEEGVKYERTFVGSEFIDWLLQEG-EAESRREAVQLCRRLLEHGIIQHVSNKHHFFDSNL- 81


                  ....
gi 1039748751 212 FYRF 215
Cdd:cd04441    82 LYQF 85
DEP_DEPDC5-like cd04449
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in ...
 146-199 2.15e-03

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in human also known as KIAA0645, is a DEP domain containing protein of unknown function.


Pssm-ID: 239896  Cd Length: 83  Bit Score: 38.03  E-value: 2.15e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1039748751 146 LIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHV 199
Cdd:cd04449    15 GIFDRSWHKGLPSNCFIGSEAVSWLINNFEDVDTREEAVELGQELMNEGLIEHV 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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