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Conserved domains on  [gi|46195800|ref|NP_033085|]
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ral guanine nucleotide dissociation stimulator-like 2 isoform 1 [Mus musculus]

Protein Classification

RasGEF and RA_RGL2 domain-containing protein( domain architecture ID 13899042)

protein containing domains REM, RasGEF, and RA_RGL2

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
239-513 1.97e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


:

Pssm-ID: 214539  Cd Length: 242  Bit Score: 262.95  E-value: 1.97e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    239 VLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHSHLcpSVRATVTQFNKVAGAVVSSVLGATSigegprev 318
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPL--NLEAFIRRFNEVSNWVATEILKQTT-------- 70

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    319 tvrplrPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSR 398
Cdd:smart00147  71 ------PKDRAELLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYR 144

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    399 ELLMQEVKPqppvephskkaprsgfrggGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKecR 478
Cdd:smart00147 145 EALSSCNLP-------------------PCIPFLGVLLKDLTFIDEGNPDFLENGLVNFEKRRQIAEILREIRQLQS--Q 203

                          250       260       270
                   ....*....|....*....|....*....|....*...
gi 46195800    479 GYDLRPN-SDIQQWLQGLQPL--TEAQSHRVSCEVEPP 513
Cdd:smart00147 204 PYNLRPNrSDIQSLLQQLLDHldEEEELYQLSLKIEPR 241
RA_RGL2 cd17211
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 ...
 649-734 7.14e-46

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 (RalGDS-like 2) and similar proteins; RalGDS-like 2 (RGL2), also termed RalGDS-like factor (RLF), or Ras-associated protein RAB2L, is a novel Ras and Rap 1A-associating protein that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL2 exhibits guanine nucleotide exchange activity towards the small GTPase Ral. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair. The RA domain of RGL2 is phosphorylated by protein kinase A and the phosphorylation affects the ability of RGL2 to bind both Ras and Rap1.


:

Pssm-ID: 340731  Cd Length: 86  Bit Score: 158.45  E-value: 7.14e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELGeDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMDGASHD 728
Cdd:cd17211   2 DCRIIRVRMELH-DGSVYKSILVTSQDKTPAVISRALEKHNQSSQAASPYELVQLLPEGKELTIPPTANVFYAMSSASLD 80


                ....*.
gi 46195800 729 FLLRQR 734
Cdd:cd17211  81 FILRPR 86
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 90-212 2.60e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.60e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800     90 RRLRAGTLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEALESH----PPGELERTTGVAISVLST 165
Cdd:smart00229   3 GLIKGGTLEALIEHLTEAFD-KADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEswveEKVNPRRVKNRVLNILRT 81

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*..
gi 46195800    166 WLASHPEDFGSEVKGQLDRLESFLLRTGYaAREGVVGGSADLIRNLR 212
Cdd:smart00229  82 WVENYWEDFEDDPKLISFLLEFLELVDDE-KYPGLVTSLLNLLRRLS 127
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
239-513 1.97e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 262.95  E-value: 1.97e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    239 VLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHSHLcpSVRATVTQFNKVAGAVVSSVLGATSigegprev 318
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPL--NLEAFIRRFNEVSNWVATEILKQTT-------- 70

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    319 tvrplrPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSR 398
Cdd:smart00147  71 ------PKDRAELLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYR 144

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    399 ELLMQEVKPqppvephskkaprsgfrggGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKecR 478
Cdd:smart00147 145 EALSSCNLP-------------------PCIPFLGVLLKDLTFIDEGNPDFLENGLVNFEKRRQIAEILREIRQLQS--Q 203

                          250       260       270
                   ....*....|....*....|....*....|....*...
gi 46195800    479 GYDLRPN-SDIQQWLQGLQPL--TEAQSHRVSCEVEPP 513
Cdd:smart00147 204 PYNLRPNrSDIQSLLQQLLDHldEEEELYQLSLKIEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 239-509 2.32e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 259.88  E-value: 2.32e-81
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 239 VLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHshLCPSVRATVTQFNKVAGAVVSSVLGATSigegprev 318
Cdd:cd00155   1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKNIH--LSPNLERFIERFNNLSNWVASEILLCTN-------- 70

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 319 tvrplrPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSR 398
Cdd:cd00155  71 ------PKKRARLLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYR 144

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 399 ELLMqEVKPQPPvephskkaprsgfrgggVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKecR 478
Cdd:cd00155 145 KLLK-SVGPNPP-----------------CVPFLGVYLKDLTFLHEGNPDFLEGNLVNFEKRRKIAEILREIRQLQS--N 204

                       250       260       270
                ....*....|....*....|....*....|...
gi 46195800 479 GYDLRPNSDIQQWLQGL--QPLTEAQSHRVSCE 509
Cdd:cd00155 205 SYELNRDEDILAFLWKLleLILNEDELYELSLE 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 246-461 2.23e-60

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 201.67  E-value: 2.23e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   246 HLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHShlcPSVRATVTQFNKVAGAVVSSVLgatsigegprevtvRPLRP 325
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENS---PNIEAMIARFNKLSNWVASEIL--------------SEEDL 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   326 PQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSRELLMQEV 405
Cdd:pfam00617  64 KKRAKVIKKFIKIAEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSAS 143

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 46195800   406 KPqppvephskkaprsgfrgggVVPYLGTFLKDLVMLDAASKDELENGYINFDKRR 461
Cdd:pfam00617 144 PP--------------------CIPFLGLYLTDLTFIEEGNPDFLEGGLINFEKRR 179
RA_RGL2 cd17211
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 ...
 649-734 7.14e-46

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 (RalGDS-like 2) and similar proteins; RalGDS-like 2 (RGL2), also termed RalGDS-like factor (RLF), or Ras-associated protein RAB2L, is a novel Ras and Rap 1A-associating protein that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL2 exhibits guanine nucleotide exchange activity towards the small GTPase Ral. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair. The RA domain of RGL2 is phosphorylated by protein kinase A and the phosphorylation affects the ability of RGL2 to bind both Ras and Rap1.


Pssm-ID: 340731  Cd Length: 86  Bit Score: 158.45  E-value: 7.14e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELGeDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMDGASHD 728
Cdd:cd17211   2 DCRIIRVRMELH-DGSVYKSILVTSQDKTPAVISRALEKHNQSSQAASPYELVQLLPEGKELTIPPTANVFYAMSSASLD 80


                ....*.
gi 46195800 729 FLLRQR 734
Cdd:cd17211  81 FILRPR 86
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 90-212 2.60e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.60e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800     90 RRLRAGTLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEALESH----PPGELERTTGVAISVLST 165
Cdd:smart00229   3 GLIKGGTLEALIEHLTEAFD-KADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEswveEKVNPRRVKNRVLNILRT 81

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*..
gi 46195800    166 WLASHPEDFGSEVKGQLDRLESFLLRTGYaAREGVVGGSADLIRNLR 212
Cdd:smart00229  82 WVENYWEDFEDDPKLISFLLEFLELVDDE-KYPGLVTSLLNLLRRLS 127
RA smart00314
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 649-735 1.74e-18

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Kalhammer et al. have shown that not all RA domains bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase. Predicted RA domains in PLC210 and nore1 found to bind RasGTP. Included outliers (Grb7, Grb14, adenylyl cyclases etc.)


Pssm-ID: 214612  Cd Length: 90  Bit Score: 80.81  E-value: 1.74e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    649 DCRIIRVQMELGEDGSvYKSILVTSQDKAPSVISRVLKKNNRDsAVASEFELVQLLPGDRELTIPHSANVFYAM-----D 723
Cdd:smart00314   1 DTFVLRVYVDDLPGGT-YKTLRVSSRTTARDVIQQLLEKFHLT-DDPEEYVLVEVLPDGKERVLPDDENPLQLQklwprR 78

                           90
                   ....*....|..
gi 46195800    724 GASHDFLLRQRR 735
Cdd:smart00314  79 GPNLRFVLRKRD 90
RA pfam00788
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 649-736 5.18e-14

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Recent evidence (not yet in MEDLINE) shows that some RA domains do NOT bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase.


Pssm-ID: 425871  Cd Length: 93  Bit Score: 68.13  E-value: 5.18e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   649 DCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAvASEFELV-QLLPGDRELTIPHSANVFYAM----- 722
Cdd:pfam00788   1 DDGVLKVYTEDGKPGTTYKTILVSSSTTAEEVIEALLEKFGLEDD-PRDYVLVeVLERGGGERRLPDDECPLQIQlqwpr 79

                          90
                  ....*....|....
gi 46195800   723 DGASHDFLLRQRRR 736
Cdd:pfam00788  80 DASDSRFLLRKRDD 93
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 96-214 6.82e-13

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 65.90  E-value: 6.82e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800  96 TLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEALESHPPGELERTTGVA-------ISVLSTWLA 168
Cdd:cd06224   1 TLEALIEHLTSTFD-MPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPPENLEYNDWDKKKSkpirlrvLNVLRTWVE 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 46195800 169 SHPEDFGSEVKgQLDRLESFLLRTgyAAREGVVGGSADLIRNLRAR 214
Cdd:cd06224  80 NYPYDFFDDEE-LLELLEEFLNRL--VQEGALLQELKKLLRKLLKL 122
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 91-174 1.64e-12

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 64.25  E-value: 1.64e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    91 RLRAGTLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEalESHPPGELERTTGVA----------I 160
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRI-MLDDSFLSTFLLTYRSFTTPAELLELLIERYN--IPPPLDLSSDSYWISkktlpirirvL 77

                          90
                  ....*....|....
gi 46195800   161 SVLSTWLASHPEDF 174
Cdd:pfam00618  78 SVLRHWVENYFSDF 91
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
239-513 1.97e-82

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 262.95  E-value: 1.97e-82
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    239 VLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHSHLcpSVRATVTQFNKVAGAVVSSVLGATSigegprev 318
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPL--NLEAFIRRFNEVSNWVATEILKQTT-------- 70

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    319 tvrplrPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSR 398
Cdd:smart00147  71 ------PKDRAELLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYR 144

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    399 ELLMQEVKPqppvephskkaprsgfrggGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKecR 478
Cdd:smart00147 145 EALSSCNLP-------------------PCIPFLGVLLKDLTFIDEGNPDFLENGLVNFEKRRQIAEILREIRQLQS--Q 203

                          250       260       270
                   ....*....|....*....|....*....|....*...
gi 46195800    479 GYDLRPN-SDIQQWLQGLQPL--TEAQSHRVSCEVEPP 513
Cdd:smart00147 204 PYNLRPNrSDIQSLLQQLLDHldEEEELYQLSLKIEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 239-509 2.32e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 259.88  E-value: 2.32e-81
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 239 VLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHshLCPSVRATVTQFNKVAGAVVSSVLGATSigegprev 318
Cdd:cd00155   1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKNIH--LSPNLERFIERFNNLSNWVASEILLCTN-------- 70

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 319 tvrplrPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSR 398
Cdd:cd00155  71 ------PKKRARLLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYR 144

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 399 ELLMqEVKPQPPvephskkaprsgfrgggVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKecR 478
Cdd:cd00155 145 KLLK-SVGPNPP-----------------CVPFLGVYLKDLTFLHEGNPDFLEGNLVNFEKRRKIAEILREIRQLQS--N 204

                       250       260       270
                ....*....|....*....|....*....|...
gi 46195800 479 GYDLRPNSDIQQWLQGL--QPLTEAQSHRVSCE 509
Cdd:cd00155 205 SYELNRDEDILAFLWKLleLILNEDELYELSLE 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 246-461 2.23e-60

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 201.67  E-value: 2.23e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   246 HLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHShlcPSVRATVTQFNKVAGAVVSSVLgatsigegprevtvRPLRP 325
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENS---PNIEAMIARFNKLSNWVASEIL--------------SEEDL 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   326 PQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSRELLMQEV 405
Cdd:pfam00617  64 KKRAKVIKKFIKIAEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSAS 143

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 46195800   406 KPqppvephskkaprsgfrgggVVPYLGTFLKDLVMLDAASKDELENGYINFDKRR 461
Cdd:pfam00617 144 PP--------------------CIPFLGLYLTDLTFIEEGNPDFLEGGLINFEKRR 179
RA_RGL2 cd17211
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 ...
 649-734 7.14e-46

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 (RalGDS-like 2) and similar proteins; RalGDS-like 2 (RGL2), also termed RalGDS-like factor (RLF), or Ras-associated protein RAB2L, is a novel Ras and Rap 1A-associating protein that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL2 exhibits guanine nucleotide exchange activity towards the small GTPase Ral. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair. The RA domain of RGL2 is phosphorylated by protein kinase A and the phosphorylation affects the ability of RGL2 to bind both Ras and Rap1.


Pssm-ID: 340731  Cd Length: 86  Bit Score: 158.45  E-value: 7.14e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELGeDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMDGASHD 728
Cdd:cd17211   2 DCRIIRVRMELH-DGSVYKSILVTSQDKTPAVISRALEKHNQSSQAASPYELVQLLPEGKELTIPPTANVFYAMSSASLD 80


                ....*.
gi 46195800 729 FLLRQR 734
Cdd:cd17211  81 FILRPR 86
RA_RalGDS_like cd00153
Ras-associating (RA) domain of RalGDS family; The RalGDS family RA domains can interact with ...
 649-734 2.86e-35

Ras-associating (RA) domain of RalGDS family; The RalGDS family RA domains can interact with activated Ras and may function as effectors for other Ras family. Ras proteins are small GTPases that are involved in cellular signal transduction. The RA domain has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub); Ub is a protein modifier in eukaryotes and is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. The RalGDS family includes RalGDS, RGL, RGL2/Rlf and RGL3. All family members have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal RA domain. The RA domain mediates the GTP-dependent interaction with Ras and Ras-related proteins.


Pssm-ID: 340449  Cd Length: 88  Bit Score: 128.46  E-value: 2.86e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELG-EDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMDGAS- 726
Cdd:cd00153   1 DSRIIRVSLEDGsEDGNLYKSILLTNQDRTPSVIRRALEKHNLEDEDPDDFSLVQILPDDKELVIPDNANVFYAMNSSAn 80


                ....*...
gi 46195800 727 HDFLLRQR 734
Cdd:cd00153  81 LNFILRKK 88
RA_RGL3 cd17212
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 3 ...
 649-734 1.06e-28

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 3 (RalGDS-like 3) and similar proteins; RalGDS-like 3 (RGL3), also termed Ras pathway modulator (RPM), interacts in a GTP- and effector loop-dependent manner with Rit and Ras. As a novel potential effector of both p21 Ras and M-Ras, RGL3 negatively regulates Elk-1-dependent gene induction downstream of p21 Ras or mitogen activated protein/extracellular signal regulated kinase Kinase 1 (MEKK1). It also functions as a potential binding partner for Rap-family small G-proteins and profilin II. RGL3 belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier (Ubiquitination) in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340732  Cd Length: 87  Bit Score: 110.03  E-value: 1.06e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMElGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMD-GASH 727
Cdd:cd17212   2 EARVIRVSID-NDHGNLYRSILLTSQDKAPSVVQRALQKHNVPQPWARDYQLFQVLPGDRELLIPDNANVFYAMSpAAPG 80


                ....*..
gi 46195800 728 DFLLRQR 734
Cdd:cd17212  81 DFMLRRK 87
RA_RalGDS cd17209
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) ...
 649-734 5.37e-27

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) and similar proteins; RalGDS, also termed Ral guanine nucleotide exchange factor (RalGEF), is a guanine exchange factor (GEF) for the Ral family of small GTPases. It is the prototype of RalGDS family proteins that are involved in Ras and Ral signaling pathways as downstream effector proteins. RalGDS stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. It interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. Moreover, RalGDS functions as a novel interacting partner for Rab7-interacting lysosomal protein (RILP), a key regulator for late endosomal/lysosomal trafficking. RILP suppresses invasion of breast cancer cells by inhibiting the GEF activity for RalA of RalGDS. RalGDS also plays a vital role in the regulation of Ral-dependent Weibel-Palade bodies (WPB) exocytosis from endothelial cells. In addition, RalGDS couples growth factor signaling to Akt activation by promoting PDK1-induced Akt phosphorylation. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340729  Cd Length: 86  Bit Score: 105.03  E-value: 5.37e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELgEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMDG-ASH 727
Cdd:cd17209   1 DCCIIRVSLDV-DNGNMYKSILVTSQDKTPVVIRKAMAKHNLDEEEPEDYELLQILSEDRELKIPDNANVFYAMNStANY 79


                ....*..
gi 46195800 728 DFLLRQR 734
Cdd:cd17209  80 DFVLKKR 86
RA_RGL cd17210
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 1 ...
 649-734 2.21e-26

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1) and similar proteins; RalGDS-like 1 (RGL) is a Ral-specific guanine nucleotide exchange factor that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL has been identified as a possible effector protein of Ras. It also regulates c-fos promoter and the GDP/GTP exchange of Ral. Members in this family have similar structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340730  Cd Length: 87  Bit Score: 103.14  E-value: 2.21e-26
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 649 DCRIIRVQMELGeDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFELVQLLPGDRELTIPHSANVFYAMD-GASH 727
Cdd:cd17210   2 DTCIIRVSVEDN-NGNMYKSIMLTSQDKTPAVIQRAMSKHNLESDPAEDYELVQVISEDRELVIPDNANVFYAMNsSVNF 80


                ....*..
gi 46195800 728 DFLLRQR 734
Cdd:cd17210  81 DFILRKK 87
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 90-212 2.60e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.60e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800     90 RRLRAGTLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEALESH----PPGELERTTGVAISVLST 165
Cdd:smart00229   3 GLIKGGTLEALIEHLTEAFD-KADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEswveEKVNPRRVKNRVLNILRT 81

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*..
gi 46195800    166 WLASHPEDFGSEVKGQLDRLESFLLRTGYaAREGVVGGSADLIRNLR 212
Cdd:smart00229  82 WVENYWEDFEDDPKLISFLLEFLELVDDE-KYPGLVTSLLNLLRRLS 127
RA smart00314
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 649-735 1.74e-18

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Kalhammer et al. have shown that not all RA domains bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase. Predicted RA domains in PLC210 and nore1 found to bind RasGTP. Included outliers (Grb7, Grb14, adenylyl cyclases etc.)


Pssm-ID: 214612  Cd Length: 90  Bit Score: 80.81  E-value: 1.74e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    649 DCRIIRVQMELGEDGSvYKSILVTSQDKAPSVISRVLKKNNRDsAVASEFELVQLLPGDRELTIPHSANVFYAM-----D 723
Cdd:smart00314   1 DTFVLRVYVDDLPGGT-YKTLRVSSRTTARDVIQQLLEKFHLT-DDPEEYVLVEVLPDGKERVLPDDENPLQLQklwprR 78

                           90
                   ....*....|..
gi 46195800    724 GASHDFLLRQRR 735
Cdd:smart00314  79 GPNLRFVLRKRD 90
RA pfam00788
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 649-736 5.18e-14

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Recent evidence (not yet in MEDLINE) shows that some RA domains do NOT bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase.


Pssm-ID: 425871  Cd Length: 93  Bit Score: 68.13  E-value: 5.18e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800   649 DCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAvASEFELV-QLLPGDRELTIPHSANVFYAM----- 722
Cdd:pfam00788   1 DDGVLKVYTEDGKPGTTYKTILVSSSTTAEEVIEALLEKFGLEDD-PRDYVLVeVLERGGGERRLPDDECPLQIQlqwpr 79

                          90
                  ....*....|....
gi 46195800   723 DGASHDFLLRQRRR 736
Cdd:pfam00788  80 DASDSRFLLRKRDD 93
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 96-214 6.82e-13

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 65.90  E-value: 6.82e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800  96 TLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEALESHPPGELERTTGVA-------ISVLSTWLA 168
Cdd:cd06224   1 TLEALIEHLTSTFD-MPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPPENLEYNDWDKKKSkpirlrvLNVLRTWVE 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 46195800 169 SHPEDFGSEVKgQLDRLESFLLRTgyAAREGVVGGSADLIRNLRAR 214
Cdd:cd06224  80 NYPYDFFDDEE-LLELLEEFLNRL--VQEGALLQELKKLLRKLLKL 122
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 91-174 1.64e-12

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 64.25  E-value: 1.64e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800    91 RLRAGTLEALVRHLLDARTaGADMMFTPALLATHRAFTSTPALFGLVADRLEalESHPPGELERTTGVA----------I 160
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRI-MLDDSFLSTFLLTYRSFTTPAELLELLIERYN--IPPPLDLSSDSYWISkktlpirirvL 77

                          90
                  ....*....|....
gi 46195800   161 SVLSTWLASHPEDF 174
Cdd:pfam00618  78 SVLRHWVENYFSDF 91
RA cd17043
Ras-associating (RA) domain, structurally similar to a beta-grasp ubiquitin-like fold; RA ...
 652-732 7.30e-05

Ras-associating (RA) domain, structurally similar to a beta-grasp ubiquitin-like fold; RA domain-containing proteins function by interacting with Ras proteins directly or indirectly and are involved in various functions ranging from tumor suppression to being oncoproteins. Ras proteins are small GTPases that are involved in cellular signal transduction. The RA domain has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub); Ub is a protein modifier in eukaryotes that is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. RA-containing proteins include RalGDS, AF6, RIN, RASSF1, SNX27, CYR1, STE50, and phospholipase C epsilon.


Pssm-ID: 340563  Cd Length: 87  Bit Score: 41.92  E-value: 7.30e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46195800 652 IIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDsAVASEFELVQLLP-GDRELTIPHSANVFYAMDGASHD-- 728
Cdd:cd17043   1 VLKVYDDDLAPGSAYKSILVSSTTTAREVVQLLLEKYGLE-EDPEDYSLYEVSEkQETERVLHDDECPLLIQLEWGPQgt 79


                ....*..
gi 46195800 729 ---FLLR 732
Cdd:cd17043  80 efrFVLK 86
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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