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Conserved domains on  [gi|189011546|ref|NP_004306|]
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acid ceramidase isoform b [Homo sapiens]

Protein Classification

NAAA-beta and Ntn_AC_NAAA domain-containing protein( domain architecture ID 10634631)

NAAA-beta and Ntn_AC_NAAA domain-containing protein

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Ntn_AC_NAAA cd01903
AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also ...
150-395 2.70e-137

AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also known as N-acylsphingosine amidohydrolase), and N-acylethanolamine-hydrolyzing acid amidase (NAAA). AC catalyzes the hydrolysis of ceramide to sphingosine and fatty acid. Ceramide is required for the biosynthesis of most sphingolipids and plays an important role in many signal transduction pathways by inducing apoptosis and/or arresting cell growth. An inherited deficiency of AC activity leads to the lysosomal storage disorder known as Farber disease. AC is considered a "rheostat" important for maintaining the proper intracellular levels of these lipids since hydrolysis of ceramide is the only source of sphingosine in cells. NAAA is a eukaryotic glycoprotein that hydrolyzes bioactive N-acylethanolamines, including anandamide (an endocannabinoid) and N-palmitoylethanolamine (an anti-inflammatory and neuroprotective substance), to fatty acids and ethanolamine at acidic pH. NAAA shows structural and functional similarity to acid ceramidase, but lacks the ceramide-hydrolyzing activity of AC.


:

Pssm-ID: 238886  Cd Length: 231  Bit Score: 391.64  E-value: 2.70e-137
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 150 NIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFlgwninndtwvitEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTG 229
Cdd:cd01903    1 NIFYEIFTFCTSIVAQDSNGTIYHARNLDFGFF-------------EELSKLTVNVDFQRNGKIVFKGTTFAGYVGLLTG 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 230 FKPGLFSLTLNERFSINGGYLGIleWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCV 309
Cdd:cd01903   68 QKPGKFSLTINERFSLDGGYNGI--LALLKKDGIPVSWLIRETLENATSYEDAVEKLSTTPILAPAYFIVGGVKPGEGVV 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 310 ITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTL 389
Cdd:cd01903  146 ITRNRDSVADVYPLDLKNGTWFLVQTNYDRWKPPPFLDDRRTPAIKCMNALGQANISFKTLYDVLSTKPVLNKLTIYTTL 225

                 ....*.
gi 189011546 390 IDVTKG 395
Cdd:cd01903  226 MSVRTG 231
NAAA-beta pfam15508
beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of ...
61-124 4.63e-20

beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of vertebral sequences that form the beta subunit of vertebral N-acylethanolamine-hydrolyzing acid amidase, a member of the choloylglycine hydrolase acid ceramidase family. The alpha subunit is represented by family CBAH, pfam02275.


:

Pssm-ID: 434765  Cd Length: 63  Bit Score: 83.07  E-value: 4.63e-20
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 189011546   61 GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPsGKIMQVVDEKLPGLLGNF 124
Cdd:pfam15508   1 GPVPWYVVNLDLPPEERWTQVAKDYKPEIKSLIQALKDLLKSLVP-GKLVDLVDKLAADLLRYL 63
 
Name Accession Description Interval E-value
Ntn_AC_NAAA cd01903
AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also ...
150-395 2.70e-137

AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also known as N-acylsphingosine amidohydrolase), and N-acylethanolamine-hydrolyzing acid amidase (NAAA). AC catalyzes the hydrolysis of ceramide to sphingosine and fatty acid. Ceramide is required for the biosynthesis of most sphingolipids and plays an important role in many signal transduction pathways by inducing apoptosis and/or arresting cell growth. An inherited deficiency of AC activity leads to the lysosomal storage disorder known as Farber disease. AC is considered a "rheostat" important for maintaining the proper intracellular levels of these lipids since hydrolysis of ceramide is the only source of sphingosine in cells. NAAA is a eukaryotic glycoprotein that hydrolyzes bioactive N-acylethanolamines, including anandamide (an endocannabinoid) and N-palmitoylethanolamine (an anti-inflammatory and neuroprotective substance), to fatty acids and ethanolamine at acidic pH. NAAA shows structural and functional similarity to acid ceramidase, but lacks the ceramide-hydrolyzing activity of AC.


Pssm-ID: 238886  Cd Length: 231  Bit Score: 391.64  E-value: 2.70e-137
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 150 NIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFlgwninndtwvitEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTG 229
Cdd:cd01903    1 NIFYEIFTFCTSIVAQDSNGTIYHARNLDFGFF-------------EELSKLTVNVDFQRNGKIVFKGTTFAGYVGLLTG 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 230 FKPGLFSLTLNERFSINGGYLGIleWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCV 309
Cdd:cd01903   68 QKPGKFSLTINERFSLDGGYNGI--LALLKKDGIPVSWLIRETLENATSYEDAVEKLSTTPILAPAYFIVGGVKPGEGVV 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 310 ITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTL 389
Cdd:cd01903  146 ITRNRDSVADVYPLDLKNGTWFLVQTNYDRWKPPPFLDDRRTPAIKCMNALGQANISFKTLYDVLSTKPVLNKLTIYTTL 225

                 ....*.
gi 189011546 390 IDVTKG 395
Cdd:cd01903  226 MSVRTG 231
CBAH pfam02275
Linear amide C-N hydrolases, choloylglycine hydrolase family; This family includes several ...
159-410 8.63e-77

Linear amide C-N hydrolases, choloylglycine hydrolase family; This family includes several hydrolases which cleave carbon-nitrogen bonds, other than peptide bonds, in linear amides. These include choloylglycine hydrolase (conjugated bile acid hydrolase, CBAH) EC:3.5.1.24, penicillin acylase EC:3.5.1.11 and acid ceramidase EC:3.5.1.23. This domain forms the alpha-subunit for members from vertebral species, see family NAAA-beta, pfam15508.


Pssm-ID: 396726  Cd Length: 316  Bit Score: 240.88  E-value: 8.63e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  159 CTSIVAEDKKGHLIHGRNMDFGvflgwnINNDTWVIteqLKPLTVNLDFQRNNK-TVFKASsfagYVGMLTGFKPG-LFS 236
Cdd:pfam02275   1 CTSITLETKKGNLLFGRNMDFG------ISYGEEVI---ITPRNYKLVFEKLGNmLVTKYA----VIGMGTDVGSYpLFY 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  237 LTLNER-FSINGGYL-GILEWILG-KKDVMWI--GFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGN--------- 302
Cdd:pfam02275  68 DGLNEKgLGIAGLYFpGYAFYSKGpKKDKVNIqpGELILWVLGNFTSVEEVKELLTKLNIVNEALDILGGKaplhwiisd 147
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  303 QSGEGCVItRDRKESLDVYEL----DAKQGRWYVVQTNYDRWK-------HPFFLDDRR-----------------TPA- 353
Cdd:pfam02275 148 ASGESIVI-EPRKEGLKVYDNevgvMTNSPTFDWHLTNLNNYTglrpnqpQNFFMGDLDltpfgqgtgglglpgdfTPAs 226
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  354 --------KMCLNRTSQENISFETMYDVLSTKP-----VLN-----KLTVYTTLIDVTKGQ--FETYLR----------- 402
Cdd:pfam02275 227 rfvraaylKMNLPKAKTETESVATFFHILSNVAipkgaVLNiegklEYTVYTSCMDLTKGNyyFETYDNsqinavnldhe 306
                         330
                  ....*....|
gi 189011546  403 --DCPDPCIG 410
Cdd:pfam02275 307 nlDCTELVTY 316
NAAA-beta pfam15508
beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of ...
61-124 4.63e-20

beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of vertebral sequences that form the beta subunit of vertebral N-acylethanolamine-hydrolyzing acid amidase, a member of the choloylglycine hydrolase acid ceramidase family. The alpha subunit is represented by family CBAH, pfam02275.


Pssm-ID: 434765  Cd Length: 63  Bit Score: 83.07  E-value: 4.63e-20
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 189011546   61 GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPsGKIMQVVDEKLPGLLGNF 124
Cdd:pfam15508   1 GPVPWYVVNLDLPPEERWTQVAKDYKPEIKSLIQALKDLLKSLVP-GKLVDLVDKLAADLLRYL 63
C45_proenzyme NF040521
C45 family autoproteolytic acyltransferase/hydolase; Members of this family include hydrolases ...
108-397 2.72e-19

C45 family autoproteolytic acyltransferase/hydolase; Members of this family include hydrolases and N-acyltransferases, and belong to the Ntn (N-terminal nucleophile) hydrolase family. Members have an invariant Cys residue (Cys-103 in XP_002569112.1) required both for autoproteolytic processing into alpha and beta chains and for activity. The family is described by MEROPs as a cysteine protease, family C45, because of its autoproteolytic activity. Characterized members include TAN from Drosophila, which removes beta-alanine from both carcinine and N-beta-alanyl dopamine, and isopenicillin-N N-acyltransferase from various fungi. The latter has been heavily studied because of its role in penicillin biosynthesis.


Pssm-ID: 439736 [Multi-domain]  Cd Length: 312  Bit Score: 87.72  E-value: 2.72e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 108 KIMQVVDEKLPGLLgnfpgpfeEEMKGIAAVTDIPLGEIISFNIFYELFTI---CTSIVAEDKKGHLIHGRNMDFGVflg 184
Cdd:NF040521  44 EFLAALEAFAPELW--------EELEGIADGLGLPFEDVLALNARTEILAApdgCSTFAVLGEDGEPILARNYDWHP--- 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 185 wninndtwviteQLKPLTVNLDFQRNNKTVFKASSFAGYV-GMLTGFkpglfsltlNER---FSINGgylgilewILGKK 260
Cdd:NF040521 113 ------------ELYDGCLLLTIRPDGGPRYASIGYAGLLpGRTDGM---------NEAglaVTLNF--------LDGRK 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 261 DVMW---IGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGnQSGEGCVI--TRDRKESLDVYEldakqgrWYVVQT 335
Cdd:NF040521 164 LPGVgvpVHLLARAILENCKTVDEAIALLKEIPRASSFNLTLAD-ASGRAASVeaSPDRVVVVRPED-------GLLVHT 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 336 N-----YDRWKHPFFLDD--RRtpaKMCLNRTSQENISFETMYDVLST---KPVLNKL--------TVYTTLIDVTKGQF 397
Cdd:NF040521 236 NhflspELEEENRIATPSsrER---YERLEELLKGKLDAEDAKALLSDgypLPICRHPypdgdrfgTLATVVFDPAAGTL 312
YxeI COG3049
Penicillin V acylase or related amidase, Ntn superfamily [Cell wall/membrane/envelope ...
159-301 3.21e-05

Penicillin V acylase or related amidase, Ntn superfamily [Cell wall/membrane/envelope biogenesis, General function prediction only];


Pssm-ID: 225591  Cd Length: 353  Bit Score: 45.50  E-value: 3.21e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 159 CTSIVAEDKKGHLIHGRNMDfgvflgWNINNDTWVITeqlkpltvnldFQRNNKTVFKASSFA-------GYVGMlTGFK 231
Cdd:COG3049   23 CTSFTLETSDGGVVLGRTMD------WAFDLGTELWV-----------FPRGYKRTGPDGANGikwqskyGSLGM-GAYG 84
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 189011546 232 PGLFSLTLNER-FSINGGYLGILEW---ILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGG 301
Cdd:COG3049   85 IGHTTDGMNEKgLAANTLYFPEADYgdaIKKKKPGISPGEWLQWVLDNFATVEEVKQAIDKIPFVIVALNDPGE 158
 
Name Accession Description Interval E-value
Ntn_AC_NAAA cd01903
AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also ...
150-395 2.70e-137

AC_NAAA This conserved domain includes two closely related proteins, acid ceramidase (AC, also known as N-acylsphingosine amidohydrolase), and N-acylethanolamine-hydrolyzing acid amidase (NAAA). AC catalyzes the hydrolysis of ceramide to sphingosine and fatty acid. Ceramide is required for the biosynthesis of most sphingolipids and plays an important role in many signal transduction pathways by inducing apoptosis and/or arresting cell growth. An inherited deficiency of AC activity leads to the lysosomal storage disorder known as Farber disease. AC is considered a "rheostat" important for maintaining the proper intracellular levels of these lipids since hydrolysis of ceramide is the only source of sphingosine in cells. NAAA is a eukaryotic glycoprotein that hydrolyzes bioactive N-acylethanolamines, including anandamide (an endocannabinoid) and N-palmitoylethanolamine (an anti-inflammatory and neuroprotective substance), to fatty acids and ethanolamine at acidic pH. NAAA shows structural and functional similarity to acid ceramidase, but lacks the ceramide-hydrolyzing activity of AC.


Pssm-ID: 238886  Cd Length: 231  Bit Score: 391.64  E-value: 2.70e-137
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 150 NIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFlgwninndtwvitEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTG 229
Cdd:cd01903    1 NIFYEIFTFCTSIVAQDSNGTIYHARNLDFGFF-------------EELSKLTVNVDFQRNGKIVFKGTTFAGYVGLLTG 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 230 FKPGLFSLTLNERFSINGGYLGIleWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCV 309
Cdd:cd01903   68 QKPGKFSLTINERFSLDGGYNGI--LALLKKDGIPVSWLIRETLENATSYEDAVEKLSTTPILAPAYFIVGGVKPGEGVV 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 310 ITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTL 389
Cdd:cd01903  146 ITRNRDSVADVYPLDLKNGTWFLVQTNYDRWKPPPFLDDRRTPAIKCMNALGQANISFKTLYDVLSTKPVLNKLTIYTTL 225

                 ....*.
gi 189011546 390 IDVTKG 395
Cdd:cd01903  226 MSVRTG 231
CBAH pfam02275
Linear amide C-N hydrolases, choloylglycine hydrolase family; This family includes several ...
159-410 8.63e-77

Linear amide C-N hydrolases, choloylglycine hydrolase family; This family includes several hydrolases which cleave carbon-nitrogen bonds, other than peptide bonds, in linear amides. These include choloylglycine hydrolase (conjugated bile acid hydrolase, CBAH) EC:3.5.1.24, penicillin acylase EC:3.5.1.11 and acid ceramidase EC:3.5.1.23. This domain forms the alpha-subunit for members from vertebral species, see family NAAA-beta, pfam15508.


Pssm-ID: 396726  Cd Length: 316  Bit Score: 240.88  E-value: 8.63e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  159 CTSIVAEDKKGHLIHGRNMDFGvflgwnINNDTWVIteqLKPLTVNLDFQRNNK-TVFKASsfagYVGMLTGFKPG-LFS 236
Cdd:pfam02275   1 CTSITLETKKGNLLFGRNMDFG------ISYGEEVI---ITPRNYKLVFEKLGNmLVTKYA----VIGMGTDVGSYpLFY 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  237 LTLNER-FSINGGYL-GILEWILG-KKDVMWI--GFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGN--------- 302
Cdd:pfam02275  68 DGLNEKgLGIAGLYFpGYAFYSKGpKKDKVNIqpGELILWVLGNFTSVEEVKELLTKLNIVNEALDILGGKaplhwiisd 147
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  303 QSGEGCVItRDRKESLDVYEL----DAKQGRWYVVQTNYDRWK-------HPFFLDDRR-----------------TPA- 353
Cdd:pfam02275 148 ASGESIVI-EPRKEGLKVYDNevgvMTNSPTFDWHLTNLNNYTglrpnqpQNFFMGDLDltpfgqgtgglglpgdfTPAs 226
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546  354 --------KMCLNRTSQENISFETMYDVLSTKP-----VLN-----KLTVYTTLIDVTKGQ--FETYLR----------- 402
Cdd:pfam02275 227 rfvraaylKMNLPKAKTETESVATFFHILSNVAipkgaVLNiegklEYTVYTSCMDLTKGNyyFETYDNsqinavnldhe 306
                         330
                  ....*....|
gi 189011546  403 --DCPDPCIG 410
Cdd:pfam02275 307 nlDCTELVTY 316
Ntn_CGH_like cd01935
Choloylglycine hydrolase (CGH)_like. This family of choloylglycine hydrolase-like proteins ...
159-391 2.22e-48

Choloylglycine hydrolase (CGH)_like. This family of choloylglycine hydrolase-like proteins includes conjugated bile acid hydrolase (CBAH), penicillin V acylase (PVA), acid ceramidase (AC), and N-acylethanolamine-hydrolyzing acid amidase (NAAA) which cleave non-peptide carbon-nitrogen bonds in bile salt constituents. These enzymes have an N-terminal nucleophilic cysteine, as do other members of the Ntn hydrolase family to which they belong. This nucleophilic cysteine is exposed by post-translational prossessing of the precursor protein.


Pssm-ID: 238910  Cd Length: 229  Bit Score: 164.45  E-value: 2.22e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 159 CTSIVAEDKKGHLIHGRNMDFGVFLGWNinndtwviteqlkPLTVNLDFQRNNKT---------VFKASSFAGYVGMLTG 229
Cdd:cd01935    1 CTSIVAQTKDGGVYLGRNMDFSFDYELR-------------LLVFPRGYQRNGQTgdkskwyakYGSGGTSAGYIGLVDG 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 230 FKPGLFSLTLNERFSINGGYLGILEwilgKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKI----------LAPAYFIL 299
Cdd:cd01935   68 MNEKGLSVSLLYFPGYAYYPAGIKE----GKDGLPAFELIRWVLENCDSVEEVKEALKKIPIvdfpiplggpAAPLHYIL 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 300 GGNqSGEGCVITRDRKESLDVYeldakqGRWYVVQTNYDRWKHPFfldDRRTPAKMCLNRTSQEN----ISFETMYDVLS 375
Cdd:cd01935  144 SDK-SGDSAVIEPIDGGLKIYD------NPWFGVMTNHPTFDWHL---PRRFVRVAYLKNTAQKNketvEDVKNLFHILE 213
                        250
                 ....*....|....*.
gi 189011546 376 TKPVLNKLTVYTTLID 391
Cdd:cd01935  214 SVPIPNGLTVYTTVMD 229
NAAA-beta pfam15508
beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of ...
61-124 4.63e-20

beta subunit of N-acylethanolamine-hydrolyzing acid amidase; NAAA-beta is a family of vertebral sequences that form the beta subunit of vertebral N-acylethanolamine-hydrolyzing acid amidase, a member of the choloylglycine hydrolase acid ceramidase family. The alpha subunit is represented by family CBAH, pfam02275.


Pssm-ID: 434765  Cd Length: 63  Bit Score: 83.07  E-value: 4.63e-20
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 189011546   61 GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPsGKIMQVVDEKLPGLLGNF 124
Cdd:pfam15508   1 GPVPWYVVNLDLPPEERWTQVAKDYKPEIKSLIQALKDLLKSLVP-GKLVDLVDKLAADLLRYL 63
C45_proenzyme NF040521
C45 family autoproteolytic acyltransferase/hydolase; Members of this family include hydrolases ...
108-397 2.72e-19

C45 family autoproteolytic acyltransferase/hydolase; Members of this family include hydrolases and N-acyltransferases, and belong to the Ntn (N-terminal nucleophile) hydrolase family. Members have an invariant Cys residue (Cys-103 in XP_002569112.1) required both for autoproteolytic processing into alpha and beta chains and for activity. The family is described by MEROPs as a cysteine protease, family C45, because of its autoproteolytic activity. Characterized members include TAN from Drosophila, which removes beta-alanine from both carcinine and N-beta-alanyl dopamine, and isopenicillin-N N-acyltransferase from various fungi. The latter has been heavily studied because of its role in penicillin biosynthesis.


Pssm-ID: 439736 [Multi-domain]  Cd Length: 312  Bit Score: 87.72  E-value: 2.72e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 108 KIMQVVDEKLPGLLgnfpgpfeEEMKGIAAVTDIPLGEIISFNIFYELFTI---CTSIVAEDKKGHLIHGRNMDFGVflg 184
Cdd:NF040521  44 EFLAALEAFAPELW--------EELEGIADGLGLPFEDVLALNARTEILAApdgCSTFAVLGEDGEPILARNYDWHP--- 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 185 wninndtwviteQLKPLTVNLDFQRNNKTVFKASSFAGYV-GMLTGFkpglfsltlNER---FSINGgylgilewILGKK 260
Cdd:NF040521 113 ------------ELYDGCLLLTIRPDGGPRYASIGYAGLLpGRTDGM---------NEAglaVTLNF--------LDGRK 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 261 DVMW---IGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGnQSGEGCVI--TRDRKESLDVYEldakqgrWYVVQT 335
Cdd:NF040521 164 LPGVgvpVHLLARAILENCKTVDEAIALLKEIPRASSFNLTLAD-ASGRAASVeaSPDRVVVVRPED-------GLLVHT 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 336 N-----YDRWKHPFFLDD--RRtpaKMCLNRTSQENISFETMYDVLST---KPVLNKL--------TVYTTLIDVTKGQF 397
Cdd:NF040521 236 NhflspELEEENRIATPSsrER---YERLEELLKGKLDAEDAKALLSDgypLPICRHPypdgdrfgTLATVVFDPAAGTL 312
YxeI COG3049
Penicillin V acylase or related amidase, Ntn superfamily [Cell wall/membrane/envelope ...
159-301 3.21e-05

Penicillin V acylase or related amidase, Ntn superfamily [Cell wall/membrane/envelope biogenesis, General function prediction only];


Pssm-ID: 225591  Cd Length: 353  Bit Score: 45.50  E-value: 3.21e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 189011546 159 CTSIVAEDKKGHLIHGRNMDfgvflgWNINNDTWVITeqlkpltvnldFQRNNKTVFKASSFA-------GYVGMlTGFK 231
Cdd:COG3049   23 CTSFTLETSDGGVVLGRTMD------WAFDLGTELWV-----------FPRGYKRTGPDGANGikwqskyGSLGM-GAYG 84
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 189011546 232 PGLFSLTLNER-FSINGGYLGILEW---ILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGG 301
Cdd:COG3049   85 IGHTTDGMNEKgLAANTLYFPEADYgdaIKKKKPGISPGEWLQWVLDNFATVEEVKQAIDKIPFVIVALNDPGE 158
Ntn_PVA cd00542
Penicillin V acylase (PVA), also known as conjugated bile salt acid hydrolase (CBAH), ...
159-187 9.27e-05

Penicillin V acylase (PVA), also known as conjugated bile salt acid hydrolase (CBAH), catalyzes the hydrolysis of penicillin V to yield 6-amino penicillanic acid (6-APA), an important key intermediate of semisynthetic penicillins. PVA has an N-terminal nucleophilic cysteine, as do other members of the Ntn hydrolase family to which PVA belongs. This nucleophilic cysteine is exposed by post-translational prossessing of the PVA precursor. PVA forms a homotetramer.


Pssm-ID: 238303  Cd Length: 303  Bit Score: 44.13  E-value: 9.27e-05
                         10        20
                 ....*....|....*....|....*....
gi 189011546 159 CTSIVAEDKKGHLIHGRNMDFGVFLGWNI 187
Cdd:cd00542    1 CTSLTLSTKDGDHVFGRTMDFAFDLGSQI 29
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.20
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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