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Conserved domains on  [gi|92110021|ref|NP_001035214|]
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lysosomal amino acid transporter 1 homolog isoform 1 [Homo sapiens]

Protein Classification

PQ-loop repeat-containing protein( domain architecture ID 10514679)

PQ-loop repeat-containing protein functions as a membrane-bound transporter

CATH:  1.20.1280.290
SCOP:  3002472

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
183-238 5.05e-12

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


:

Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 59.82  E-value: 5.05e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 92110021   183 IGFVIGSISSVLYLLSRLPQIRTNFLRKSTQGISYSLFALVMLGNTLYGLSVLLKN 238
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNG 56
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
37-98 1.37e-08

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


:

Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 50.19  E-value: 1.37e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 92110021    37 ASVGLGLISILCFAASTFPQFIKAYKTGNMDQaLSLWFLLGWIGGDSCNLIGSFLADQLPLQ 98
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEG-LSILFLLLWLLGDIFYLLYLILNGFPPSL 61
 
Name Accession Description Interval E-value
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
183-238 5.05e-12

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 59.82  E-value: 5.05e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 92110021   183 IGFVIGSISSVLYLLSRLPQIRTNFLRKSTQGISYSLFALVMLGNTLYGLSVLLKN 238
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNG 56
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
37-98 1.37e-08

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 50.19  E-value: 1.37e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 92110021    37 ASVGLGLISILCFAASTFPQFIKAYKTGNMDQaLSLWFLLGWIGGDSCNLIGSFLADQLPLQ 98
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEG-LSILFLLLWLLGDIFYLLYLILNGFPPSL 61
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
197-228 6.11e-06

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923  Cd Length: 32  Bit Score: 42.05  E-value: 6.11e-06
                           10        20        30
                   ....*....|....*....|....*....|..
gi 92110021    197 LSRLPQIRTNFLRKSTQGISYSLFALVMLGNT 228
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
52-83 1.58e-03

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923  Cd Length: 32  Bit Score: 35.51  E-value: 1.58e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 92110021     52 STFPQFIKAYKTGNMDQaLSLWFLLGWIGGDS 83
Cdd:smart00679   2 SLLPQIIKNYRRKSTEG-LSILFVLLWLLGDI 32
 
Name Accession Description Interval E-value
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
183-238 5.05e-12

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 59.82  E-value: 5.05e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 92110021   183 IGFVIGSISSVLYLLSRLPQIRTNFLRKSTQGISYSLFALVMLGNTLYGLSVLLKN 238
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNG 56
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
37-98 1.37e-08

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 427775 [Multi-domain]  Cd Length: 61  Bit Score: 50.19  E-value: 1.37e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 92110021    37 ASVGLGLISILCFAASTFPQFIKAYKTGNMDQaLSLWFLLGWIGGDSCNLIGSFLADQLPLQ 98
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEG-LSILFLLLWLLGDIFYLLYLILNGFPPSL 61
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
197-228 6.11e-06

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923  Cd Length: 32  Bit Score: 42.05  E-value: 6.11e-06
                           10        20        30
                   ....*....|....*....|....*....|..
gi 92110021    197 LSRLPQIRTNFLRKSTQGISYSLFALVMLGNT 228
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
52-83 1.58e-03

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923  Cd Length: 32  Bit Score: 35.51  E-value: 1.58e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 92110021     52 STFPQFIKAYKTGNMDQaLSLWFLLGWIGGDS 83
Cdd:smart00679   2 SLLPQIIKNYRRKSTEG-LSILFVLLWLLGDI 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.20
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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