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Conserved domains on  [gi|228480264|ref|NP_081331|]
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sperm acrosome membrane-associated protein 4 precursor [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TFP super family cl45932
three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU ...
 19-97 3.55e-32

three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU (also known as Ly-6 antigen/uPA receptor)-like extracellular domain (ECD) occurs singly in GPI-linked cell-surface glycoproteins (Ly-6 family, CD59, thymocyte B cell antigen, Sgp-2) or as three-fold repeated domain in urokinase-type plasminogen activator receptor. It is a structural domain involved in protein-protein interactions, tolerating an unusual degree of variation and binding with high specificity to a broad spectrum of targets. The snake toxin domain is present in short and long neurotoxins, cytotoxins, and short toxins, and in other miscellaneous venom peptides. The toxin acts by binding to the nicotinic acetylcholine receptors in the postsynaptic membrane of skeletal muscles and preventing the binding of acetylcholine, thereby blocking the excitation of muscles. Both the LU-like ECD and the snake toxin domain belong to three-fingered protein (TFP) fold, which is characterized by containing 70 to 100 amino acids including eight to ten cysteine residues spaced at conserved distances.


The actual alignment was detected with superfamily member cd23574:

Pssm-ID: 480272  Cd Length: 79  Bit Score: 108.61  E-value: 3.55e-32
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 228480264  19 GIKDCVFCELTDSARCPGTHMRCGDDEDCFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCN 97
Cdd:cd23574    1 GAKTCYYCDLTNSSDCPGIPMICADEEDCYTGRGAALGFPLIINKGCIEATRCGKEESVTYMGVTYNLTTYCCEGELCN 79
 
Name Accession Description Interval E-value
TFP_LU_ECD_SPACA4 cd23574
extracellular domain (ECD) found in sperm acrosome membrane-associated protein 4 (SPACA4); ...
 19-97 3.55e-32

extracellular domain (ECD) found in sperm acrosome membrane-associated protein 4 (SPACA4); SPACA4 (also called SAMP14) is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. SPACA4 contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467104  Cd Length: 79  Bit Score: 108.61  E-value: 3.55e-32
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 228480264  19 GIKDCVFCELTDSARCPGTHMRCGDDEDCFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCN 97
Cdd:cd23574    1 GAKTCYYCDLTNSSDCPGIPMICADEEDCYTGRGAALGFPLIINKGCIEATRCGKEESVTYMGVTYNLTTYCCEGELCN 79
 
Name Accession Description Interval E-value
TFP_LU_ECD_SPACA4 cd23574
extracellular domain (ECD) found in sperm acrosome membrane-associated protein 4 (SPACA4); ...
 19-97 3.55e-32

extracellular domain (ECD) found in sperm acrosome membrane-associated protein 4 (SPACA4); SPACA4 (also called SAMP14) is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. SPACA4 contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467104  Cd Length: 79  Bit Score: 108.61  E-value: 3.55e-32
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 228480264  19 GIKDCVFCELTDSARCPGTHMRCGDDEDCFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCN 97
Cdd:cd23574    1 GAKTCYYCDLTNSSDCPGIPMICADEEDCYTGRGAALGFPLIINKGCIEATRCGKEESVTYMGVTYNLTTYCCEGELCN 79
TFP_LU_ECD_Bncr cd23597
extracellular domain (ECD) found in Danio rerio protein Bouncer and similar proteins; Bouncer ...
 23-97 2.90e-10

extracellular domain (ECD) found in Danio rerio protein Bouncer and similar proteins; Bouncer (bncr) is an oocyte-expressed fertilization factor that mediates sperm-egg binding and is essential for sperm entry into the egg. It is necessary and sufficient to mediate species-specific gamete recognition and fertilization, which is essential for vertebrate species performing external fertilization. Bouncer contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467126  Cd Length: 78  Bit Score: 52.66  E-value: 2.90e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 228480264  23 CVFCELTD-SARCPGTHMRCGDDEDCFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCN 97
Cdd:cd23597    3 CLYCPLQEkEKTCTNVTTECPPQERCFSARGRYGGYHVLSAQGCMARELCGSVHSVSYRGINYNVTYSCCCWDYCN 78
TFP cd00117
three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU ...
 23-97 1.29e-03

three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU (also known as Ly-6 antigen/uPA receptor)-like extracellular domain (ECD) occurs singly in GPI-linked cell-surface glycoproteins (Ly-6 family, CD59, thymocyte B cell antigen, Sgp-2) or as three-fold repeated domain in urokinase-type plasminogen activator receptor. It is a structural domain involved in protein-protein interactions, tolerating an unusual degree of variation and binding with high specificity to a broad spectrum of targets. The snake toxin domain is present in short and long neurotoxins, cytotoxins, and short toxins, and in other miscellaneous venom peptides. The toxin acts by binding to the nicotinic acetylcholine receptors in the postsynaptic membrane of skeletal muscles and preventing the binding of acetylcholine, thereby blocking the excitation of muscles. Both the LU-like ECD and the snake toxin domain belong to three-fingered protein (TFP) fold, which is characterized by containing 70 to 100 amino acids including eight to ten cysteine residues spaced at conserved distances.


Pssm-ID: 467060  Cd Length: 81  Bit Score: 35.54  E-value: 1.29e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 228480264  23 CVFCELTDSARCPGTHMRCGDDED-CFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCN 97
Cdd:cd00117    6 CNSSNDPNCCNSSPTLVTCSSPETfCRKIVGKVGGGETLVIRGCATECECGCTECCSGTGTSGTTCTSCCDTDLCN 81
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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