Conserved Protein Domain Family
Cas9_REC

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pfam16592: Cas9_REC 
REC lobe of CRISPR-associated endonuclease Cas9
The REC lobe of Cas9 - the CRISPR-associated endonuclease Cas9 - includes the REC1 and REC2 domains. REC1 forms an elongated, alpha-helical structure consisting of 25 alpha helices and two beta-sheets, whereas REC2 inserted within REC1 adopts a six-helix bundle structure. The REC lobe and the NUC lobe of Cas9 fold to present a positively charged groove at their interface which accommodates the negatively charged sgRNA:target DNA heteroduplex. CRISPR (clustered regularly interspaced short palindromic repeat)-Cas system occurs naturally in bacteria as a defence against invasion by phages or other mobile genetic elements. Cas9 is targeted to specific genomic locations by sgRNAs or single guide RNAs, in order to complex with invading DNA in order to cleave it and render it inactive.
Statistics
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PSSM-Id: 495954
Aligned: 7 rows
Threshold Bit Score: 615.612
Created: 17-Feb-2025
Updated: 28-Apr-2025
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
WP_014082128  177 LEKSFKALNDIFENQGRDLRLVtdk-anQYVKVLSdnaKTRSDRKKELSKLLYVPidkdtdkNSKKVTGEILKAILGNKA 255  Fructilactoba...
2_pfamImport    1 FNEFKNCLLDELEINLECPSIS------EFENILKdrtFKGTQKCKKLKELCSIP-------SSDKKLDNIFKLISGLKA 67  
4_pfamImport    1 VQESFRVLLEEMYEQLGK-EWEygehfdDIQRILE---NTTIGKKSKQEQIAAILte---qyGNKKLANEVTRACLGYSF 73  
3_pfamImport    1 VFTQFADFNNIPYEDDEKKNL-------EILEILK----KPLSKKAKVDEVMALIap---ekDFKSAYKELVTGIAGNKM 66  
1_pfamImport    1 IEMLHQSMVNILNINLKNVEIE------KIEKILK---STQYSNSERLENLLELYel---skNKNKIETEIFKFICGLKG 68  
Q8E042        182 ISKQYQDFLEIFNTTFENNDLLsq--nvDVEAILT----DKISKSAKKDRILAQYpn----qkSTGIFAEFLKLIVGNQA 251  Streptococcus...
Q8DTE3        181 VQRLFQEFLAVYDNTFENSSLQeq--nvQVEEILT----DKISKSAKKDRVLKLFpn----ekSNGRFAEFLKLIVGNQA 250  Streptococcus...
WP_014082128  256 KFDVIFgiE---VENPKEWVLTFNSDDFDDKIEGLSSQMtDEDSEILLILKELYFALNLSDILnnsvtdKNAKTLSEAMI 332  Fructilactoba...
2_pfamImport   68 DLSIIF--EdesFKENEHNKISFADSNYEETREVLNSEI-QEKTLIIDIFNSIYNWVILSDILsgg-eyNGGLYLSIAKV 143 
4_pfamImport   74 DLTVVMenEnllDESGKKIKISFSDSKYEEQELELQNRL-EEKFAVIEALKRIYNWLVLKDTL------RGKKYISEAMV 146 
3_pfamImport   67 NVTKMIlcEp-iKQGDSEIKLKFSDSNYDDQFSEVENDL-GEYVEFIDSLHNIYSWVELQTIMga--thTYNASISEAMV 142 
1_pfamImport   69 TISKIYv-EdkfEDEFAKMTLSFRDSNFDEKIIEIEDNLdDDKYDMFLLIKQIHDWSVLANIM------NGEEYLSVARV 141 
Q8E042        252 DFKKYFnlE-------DKTPLQFAKDSYDEDLENLLGQIgDEFADLFSAAKKLYDSVLLSGILtvi-dlSTKAPLSASMI 323  Streptococcus...
Q8DTE3        251 DFKKHFelE-------EKAPLQFSKDTYEEELEVLLAQIgDNYAELFLSAKKLYDSILLSGILtvt-dvGTKAPLSASMI 322  Streptococcus...
WP_014082128  333 NRYDDHKNQLAMLKKVI--DSSdKKHA------KELKEAYSAYID-GENNK--------KISFEDLKKRIQKNLVDsELS 395  Fructilactoba...
2_pfamImport  144 NTYNKHSKDLKILKSVIkkYFP-QKYTdi--faSNKENNYCSYIGmYKSGGkkysvkkcSDKPEEFYKTIKNIINK-NTA 219 
4_pfamImport  147 NKYEAHHEDLVKLKRLFrtYLStEEYScffrkeKQKDKYISNYVNyRKGAE--------RCSKEKLYKAIHELFDTvAKD 218 
3_pfamImport  143 SRYNKHHEDLQLLKKCIkdNVP-KKYFdmfrndSEKLKGYYNYIN---HPS--------KAPVDEFYKYVKKCIEKvDTP 210 
1_pfamImport  142 KLYDKHKKDLEVLKKYYkqNSM-EEYNkm--frQMNDGNYSAYVG-SVIYKdnsvrrgcKSKKEDFYKNILNTIKSwEDC 217 
Q8E042        324 QRYDEHREDLKQLKQFVkaSLP-EKYQei--faDSSKDGYAGYIE-----G--------KTNQEAFYKYLSKLLTKqEDS 387  Streptococcus...
Q8DTE3        323 QRYNEHQMDLAQLKQFIrqKLS-DKYNev--fsDVSKDGYAGYID-----G--------KTNQEAFYKYLKGLLNKiEGS 386  Streptococcus...
WP_014082128  396 ---------QKINDLLDHDYFLPKQRSKENGAIPHQLQQQELDQIIESQKQYYPFLAElnpNEkrsrqakYKLDELVAFR 466  Fructilactoba...
2_pfamImport  220 eeisndkdvQYILSEIENNSFLPLQINKNNGVIPYQANKIELESILKNAENYLPFLKEi--DTecnkttsEKIIDLFEFR 297 
4_pfamImport  219 dv-----vyQEIVEKMDQGEFLTKQNEVSNAYIPYQCNEVEMKRILKQQGEFYPELKE---NA-------DKILQLLLFR 283 
3_pfamImport  211 e-------aKQILHDIELENFLLKQNSRTNGSVPYQMQLDEMIKIIDNQAKYYPVLKE---KR-------EQLLSILTFK 273 
1_pfamImport  218 ea------kEYITSEIDKGNFLPKQITASNGVIPNQVHKKELKKILKNASEYLNFLNE---KDesgytisEKIVKLFEFQ 288 
Q8E042        388 ---------ENFLEKIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKE---NQ-------DRIEKILTFR 448  Streptococcus...
Q8DTE3        387 ---------GYFLDKIEREDFLRKQRTFDNGSIPHQIHLQEMRAIIRRQAEFYPFLAD---NQ-------DRIEKLLTFR 447  Streptococcus...
WP_014082128  467 VPYYVGPMvevtdsnQENNGKFAWMIR---KEQGEITPWNFDEKVDRTETATKFITRMTTTDTYLIGEPVLPKNSLLYQK 543  Fructilactoba...
2_pfamImport  298 IPYYVGPL-------NTSKGENAWMIR---KEDGTIRPWNFEQKVDIDKTAAEFITRMTNQCTYLNNETVLPKYSLLYSE 367 
4_pfamImport  284 IPYYVGPLv------DEKQSKFAWMKRrtgMEQVKIYPWNFEQVVDIDQTAEAFITRMTNYCTYLPEEKVLPKQSLLYSE 357 
3_pfamImport  274 IPYYFGPL--------NETSEHAWIKRlegKENQRILPWNYQDIVDVDATAEGFIKRMRSYCTYFPDEEVLPKNSLIVSK 345 
1_pfamImport  289 IPYYVGPIaynigndESKHRHNMWSVR---KEKGPIYPWNFEQKIDIKKSSEKFIRNLINHCTYLNDEEVLPKNSLLYEK 365 
Q8E042        449 IPYYIGPL-------AREKSDFAWMTR---KTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDFYLPEEKVLPKHSLIYEK 518  Streptococcus...
Q8DTE3        448 IPYYVGPL-------ARGKSDFAWLSR---KSADKITPWNFDEIVDKESSAEAFINRMTNYDLYLPNQKVLPKHSLLYEK 517  Streptococcus...
WP_014082128  544 FVVLNELNNLKINQE-----IITPGQKQYLYEHVMKKNKRVTLKKIADALVIKGD------------YPYAPEVKGTTDQ 606  Fructilactoba...
2_pfamImport  368 YTVLNEINNIRINGE-----KISVELKQDIFNILFKNNKQVTCKKLLSHLNSNGY------------NLKKDDLSGFDGN 430 
4_pfamImport  358 YELLQELNKIKINGK------FSLETRNKIIEQLFKKQKKVTEKQLKKWLVREQYcttkvtekmvklDENAYYITGFQKE 431 
3_pfamImport  346 YEVYNELNKIRVDDK-----LLEVDIKNDIYNELFMNNKTVTEKKLKNWLVNNQC------------CNKNAEIKGFQKE 408 
1_pfamImport  366 FMVLNELNKLKINGE-----KISVELKQNIFNDLFKKGKKVTKKGLIKYLKEQGEi----------dNCEEVEISGIDGD 430 
Q8E042        519 FTVYNELTKVRYKNEqgetyFFDSNIKQEIFDGVFKEHRKVSKKKLLDFLAKEYE------------EFRIVDVIGLDKE 586  Streptococcus...
Q8DTE3        518 FTVYNELTKVKYKTEqgktaFFDANMKQEIFDGVFKVYRKVTKDKLMDFLEKEFD------------EFRIVDLTGLDKE 585  Streptococcus...
WP_014082128  607 k-nLNNGLTTYIDLAK-IF-GNELDDVDREADFEKIIEWSTIF-EDQHIFALKLQE--LKWLTEKQRNQVVKLRYQGWGK 680  Fructilactoba...
2_pfamImport  431 ---FKSSLSSYIDLKNyIF-GDNIDKPQIYNLAEKIILWITLYgDDSKLLKRVLQKncSEQLNPEQIKKITKLKFKGWGR 506 
4_pfamImport  432 n-eFASSLTSYIDFTK-IL-GE--ITERNRKMIEEIIYWLTVF-EEKSIVKRKIQKkyGNEINDKQLAQILKLNYSGWGR 505 
3_pfamImport  409 n-qFSTSLTPWIDFTN-IF-GE--INQSNFDLIEDIIYDLTVF-EDKKIMKRRLKKkyA--LPDDKIKQILKLKYKDWSR 480 
1_pfamImport  431 ---FTNKLSNYKKFAD-IF-GVQSLTYEQTDIAENIIRYSTIYgDSRKFLEERIREeySNVLDEKQIKRILGMKFKDWGR 505 
Q8E042        587 nkaFNASLGTYHDLEK-ILdKDFLDNPDNESILEDIVQTLTLF-EDREMIKKRLENy-KDLFTESQLKKLYRRHYTGWGR 663  Streptococcus...
Q8DTE3        586 nkvFNASYGTYHDLCK-ILdKDFLDNSKNEKILEDIVLTLTLF-EDREMIRKRLENy-SDLLTKEQVKKLERRHYTGWGR 662  Streptococcus...
WP_014082128  681 LSKELLAgLVDDNGQ-----RIIDLLWSD---NQ--NFMQIVNQK--SFKESIAD 723  Fructilactobacillus sanfranciscensis
2_pfamImport  507 LSKAFLSeLRGADREtgetmTIIKGLRNT---QN--NLMQLLSQNy-TFTEECEK 555 
4_pfamImport  506 FSEKFLNgIKGFDRQgq-kkTIMELLRED---SRllNIMQIINNEemGFCKIIEA 556 
3_pfamImport  481 LSKKLLDgIV-ADNKfgssvTVLDVLEMS---RL--NLMEIINDRdlGYAQMIEA 529 
1_pfamImport  506 LSKELLE-LSGVDKEtgeiaSVISRMWND---NY--NLMELIATErfSYAYEIEQ 554 
Q8E042        664 LSAKLINgIRDKESQk----TILDYLIDDgrsNR--NFMQLINDDglSFKSIISK 712  Streptococcus agalactiae serogroup V
Q8DTE3        663 LSAELIHgIRNKESRk----TILDYLIDDgnsNR--NFMQLINDDalSFKEEIAK 711  Streptococcus mutans UA159
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