Conserved Protein Domain Family
EPOP
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pfam15223: EPOP 
Elongin BC and Polycomb repressive complex 2-associated protein
This family of proteins is found in eukaryotes. Family members are approximately 835 amino acids in length and include the polycomb repressive complex 2-associated factor EPOP (Elongin BC and Polycomb Repressive Complex 2 (PRC2) Associated Protein, also termed C17orf96, esPRC2p48, E130012A19Rik), a scaffold protein expressed in the inner cell mass of the mouse blastocyst serving as a bridging partner between the PRC2/EED-EZH2 complex and the elongin BC complex, and fine-tuning the transcriptional status of Polycomb group (PcG) target genes in embryonic stem cells. Both EPOP and Elongin BC are required to maintain low levels of expression at PRC2 genomic targets. EPOP interacts with the H2B deubiquitinase USP7 to modulate transcriptional processes in mESCs similar to MYC. Functional studies found that the C-terminal region of EPOP directly interacts with PRC2 and that this region associates with the VEFS-box of the PRC2 core member SUZ12. Thus, suggesting that EPOP may directly affect PRC2 chromatin binding by altering the functionality of Suz12. This region is proposed to have evolved in early vertebrates and is also present in the paralog SKIDA1 (C10orf140) another novel PRC2-interacting protein, supporting the idea that proteins possessing this sequence are PRC2 regulators in the entire vertebrate branch. The family is found in association with pfam02437.
Links
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Statistics
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PSSM-Id: 494918
Aligned: 4 rows
Threshold Bit Score: 465.979
Created: 16-Feb-2025
Updated: 28-Apr-2025
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Q80YR3       385 SVQAQANFLYHLAS--AAAATKPAafEDAGRLPDLKSS--VKAESPEEWSLQSWAPKGTPVYCPASLGSC-FPEIRNDRV 459 house mouse
XP_005997143 387 TLLAQASFQYQIPTktHNSDAGRL--DEISEGKPTHYD--LKFAFQEKKNGQSCLSKPQNVRYSASFGSC-FSEIKRDRI 461
2_pfamImport   2 PLLAQPSFHYNLHQrpTNQSGATLldYGIASRKLPKSD--VKLGVVKKQQDESHATKQRSLF-PAIPASC-FPESKRERE 77 
1_pfamImport   2 PLLLQPTFHYNNQPq-HDTQHHTP----SAGGDDSRHEkrQKCEFICTESGKDFGASQISKLSSAATGKSfFTEQKLQK- 75 

Q80YR3       460 SEITFPHSEIsSTVKRTDLTINchaEGASSPSPKTNNVF-PQQRILREARKCLQATPTtHCAENSTIAARFLNNdscgTT 538 house mouse
XP_005997143 462 SETAFPHTEF-SNVKNTDPTIK---ETISSPSPKKSTEL-SQERIFREAKKCVQSTGL-HGAEYDVLCGSLTND----SS 531
2_pfamImport  78 REIALSHFEIsDDSKRTDPTINsfkERGSSPCPKENKEF-PQQRILGQANKYPSAP-IsHCAEHKETIVPQLRDnnssLA 155
1_pfamImport  76 -----------ADLNRTDSSGE---LTSYLPGCN-WKAFqSPRRTTGHPTKGSSGPGApSHLDT----LPKCAE------ 130

Q80YR3       539 ANSGKDSKIPHCPEFATDLPSLQSDPRvdtaTAAAAAAAatkaeslctgTGDKTLPF-LHNIKIKVEDSSANEEYEPEL- 616 house mouse
XP_005997143 532 ENSEKESKVSSYSKLPPIFHCIKSDGE----LSAENQYE----------EAYQDLTF-PHNIKIKVEENSCNEEYDSEAq 596
2_pfamImport 156 LNFKKEVKINNCSKLPPPFNTVKTETEey-tLSTNSHIQs--------tNTGKAPTF-LHDVKIKVEDNFDE--YSSVGe 223
1_pfamImport 131 ---KREARTANQ-KPSSPVKKIKSEAEet-cVTAGPHAVn--------gRTARTPPFnLHNVKIKVEESLDEYEYQSQD- 196

Q80YR3       617 -ITNKLKWECNDAEGEFYNMTekkEEDALVpTAKEGFACPEKETPSLNPlAQSQGLSC-----TLGSPKPEDGEYKFG-- 688 house mouse
XP_005997143 597 dIKDKLKYECNVFKEELTNLNennTREALA-QAKEDFTSTEKQTTSQNVlTQSQEPPC-----TLSTPKPEDGEYKYG-- 668
2_pfamImport 224 fSETGSEYKHSVDDDDVTTNT---KCLNGA-DRRTDTLDNANKTSQKSP-TRNQEFQS-----TLRSPFPELGEYKNG-- 291
1_pfamImport 197 -----SSVKCKTDTSNG-------QHQSGA-IKQADFFSGEIKTTERGP-GVSPESPCgpqecVQEKPCTDEEKAVSKsc 262

Q80YR3       689 -ARVRKNYRTLVLGKRPVLQTPPVKPNLKSARSPRPT--GKTETHEGTLDDFTVLNRRKKVASNVASAVKRPFNFMANFP 765 house mouse
XP_005997143 669 -ARVRKNYRTLVLGKQAAQHATPVKANLKSDRSPRPA--GKTYLHEGTLEDFTVSNKRKRVASNVASAVKRPFNFMANFP 745
2_pfamImport 292 -ARVRKNYRTLVLGKHSEIQRTPLKLNVK-DRTPRST--GKNEFYEGSLEDLSSTGKRKRASSN--MTVKKPFNFMANFP 365
1_pfamImport 263 kPAGPRSKRSGVFGARTKRKVPRINQAASSCSSPSSSrpGCEDASAEDLP-----SRRKRNAGNPTSPGKPSFSLMANFP 337

Q80YR3       766 CPPSLIIGKDGDLWPAYSLNTTKDSQPPHKAHPIWKWQLGGSAIPLPPSHKFRKF 820 house mouse
XP_005997143 746 SPPSLIIGNDGDLFPAYSLNTAKDPQPPHKAHPIWKWQQGGSAIPLPPSHKFRKF 800
2_pfamImport 366 SPPSLIIGNDGDLSPAYSLNSVKDNQPPHKSHPVWKWQLGGSAVPLPPSHKFRKF 420
1_pfamImport 338 TPPSLVVGSDGDLCPAYSLNSLRGPGPPPPSHPVWRWQPGGHVLPPPQAPKTRKY 392
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