Conserved Protein Domain Family
Trs65

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pfam12735: Trs65 
TRAPP trafficking subunit Trs65
This family is one of the subunits of the TRAPP Golgi trafficking complex. TRAPP subunits are found in two different sized complexes, TRAPP I and TRAPP II. While both complexes contain the same seven subunits, Bet3p, Bet5p, Trs20p, Trs23p, Trs31p, Trs33p and Trs85p, with TRAPPC human equivalents, TRAPP II has the additional three subunits,Trs65p, Trs120p and Trs130p. While it has been implicated in cell wall biogenesis and stress response, the role of Trs65 in TRAPP II is supported by the findings that the protein co-localizes with Trs130p, and deletion of TRS65 in yeast leads to a conditional lethal phenotype if either one of the other TRAPP II-specific subunits is modified. Furthermore, the trs65 mutant has reduced Ypt31/32p guanine nucleotide exchange, GEF, activity.
Statistics
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PSSM-Id: 403823
View PSSM: pfam12735
Aligned: 58 rows
Threshold Bit Score: 152.813
Threshold Setting Gi: 444316686
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EJT73311     329 TPYFDCEVVLTKIKLGLQSGVVTDL--NSdagLSLPLSCVAHDHVTFLYGLA-----PPEHEhllegvsggwsgnsrnap 401 Gaeumannomyces ...
XP_004197047 255 QNTFPYYLSIKELQISFTEGEVKNQnhV----ETFPLDFKLKDSLSLTYKLSi----DKILDsknld----------rni 316 Pichia farinosa...
XP_001387446 254 GNTPEYYFNISSLSMDFKYGDIKEIktDS---LRLPLRFKSSDSINFTYKLTn----NENADs----------------- 309 Scheffersomyces...
EGW35356     254 EDEDDHYFNLVDVNLQFKSGSIDPIigND---LMPPTRFKYRDTISLAYKLTn----NEPLa------------------ 308 Spathaspora pas...
EMG47784     238 VNPDKFFFNILDIQVVFKSGSVESI---S---DLIPIRIKCTDSVNLAYKFTn----YE-VDa----------------- 289 Candida maltosa...
EER31758     244 KNVENFYFDIVDLNVGFKSGSVQPI---S---QLIPSKIGYSDSLNLTYKFTn----YE-ADs----------------- 295 Candida tropica...
CCE40745     231 RVGDKLYFNIESFDLSLDSCAATPM---N---EQFPLRMAFDDSVNIVYKLDn----NEQIG------------------ 282 Candida parapsi...
EDK41417     263 EDTQSYYFSILSLDVSFKYGSMVELtaSD---YKMPMRIRLADALNLTYKLVnadfeREPSDp----------------- 322 Meyerozyma guil...
Q6BQ76       255 ELKEHYYFNILNLSLDFKNGTIDDLnsFK---DKFPINFKLIDSVSLSYRLVn----NEFLEkdlkqf--------dnts 319 Debaryomyces ha...
XP_006684632 246 LHHSSLHIRLNEFELSFAG-QIINIneGI---YHFPIDFAVDESINLNFKLInn-------------------------- 295 Yamadazyma tenu...
EJT73311     402 gAPPAARDLDISIEVKAL-------------VRPGvctprMTMAWTTQLDFTP---PVN----PGFGASALTPp-MQRS- 459 Gaeumannomyces ...
XP_004197047 317 gVNSYIKAINLRIVIQLRkslaeegnavsvpISSD-----IMMNWSPSLDFSLiapPINnclkTTSTNS-PSLsqVNYQs 390 Pichia farinosa...
XP_001387446 310 nNYMFSRPVNIKLVVQIQkyipkl--dtyenLSGT-----ITTSWSPYLDFSIiapPINsslkTTNTYSQQSSasSQTQp 382 Scheffersomyces...
EGW35356     309 eNVHSSKPVHIFLMLQVQkrhng----efynISNT-----IHTEWTPYLDFGLiasPINstlkSV-----SSSlqTYLQp 374 Spathaspora pas...
EMG47784     290 kDGNSSKPIHISLTLQVQqkidg----dfqnMSNR-----IKTEWSPYLDFGLiapPINnalkTS-S---NNLahTQSQa 356 Candida maltosa...
EER31758     296 kDNNTSKPIHISLTLRVQemiqg----kllnISNT-----IQTEWSPYLDFGLiapPINnalkTSHT---NNLshIQTQg 363 Candida tropica...
CCE40745     283 --KESTKQVSITMNSTVQrnadg----vykdVSDP-----IRTNWRPCLDLGLiapPINnalkTN-----NNTphLQSQm 346 Candida parapsi...
EDK41417     323 qVNASSKALNIKLVLQVQrydcsa--nsfvnVSNE-----ITTFWAPILDFTImapPINsslrTS-----VGYsqVQSVg 390 Meyerozyma guil...
Q6BQ76       320 nMNQFSKSISIRLTLQVQkfipdl--ntfedISNV-----ITTSWSPFLDFSIiapPINnslkTT-----TNYsqFQSLp 387 Debaryomyces ha...
XP_006684632 296 eSLETSRQVTVTLNLQVLgpa-------aspYSST-----ISTSWSPLVDFNLvapPISnslkSMLTQAQPPQtlAKRR- 362 Yamadazyma tenu...
EJT73311     460 -----------------HRPSQLSIDGIasmVSPAVSRPDSLPALEAAAAR----------------------NTETIIP 500 Gaeumannomyces ...
XP_004197047 391 lkv-----------prkSNFYKIK-------NASSGSLLAGSPVLTSTNLTqpskklgfpplksassvmvnltT-SNTST 451 Pichia farinosa...
XP_001387446 383 vvptskllnstrksamiNSLYKLK--SPnp-ttYSANNIMGTLGSNSSVNTsassv------------tvnltT-NNNST 446 Scheffersomyces...
EGW35356     375 naplnts---irkptllQSMYK----GPnl-nnSSANIAM----TNTISNKnpqhqlqksrtfssgsimvslsT-NANST 441 Spathaspora pas...
EMG47784     357 tlstan----irqkvlkSNIYKLK--SAsl---sSTNNF-----SNSASNLrrakaplihpsgrsssvtvnltT-GTNSS 421 Candida maltosa...
EER31758     364 saipins---nrqkalsNNIYKLK--SAsm---sSASNV-----SSGGSNVrrlrqplihpsgrsssvtvnltT-ATSST 429 Candida tropica...
CCE40745     347 snsvngs--gnrqraalNTLYKME--SPtf-ssLNTNSV--------ISNAkraksfvare---assvtvnlsT-NINSS 409 Candida parapsi...
EDK41417     391 grglq-------rksalHSVYSIK-----------SNSNSSQLVNGTNINPpavqpglkqsplcssnvtvnlaN-NPASA 451 Meyerozyma guil...
Q6BQ76       388 nsqpfk-----ynnvkkNNLLKPKVLSSqa-siTSGNQINTGFSNFNINSTkrlskaliss--ssssvtvnltT-SNNST 458 Debaryomyces ha...
XP_006684632 363 --------------------------------LVTTGASSLSTANTLSFKKtatnpvlfrkgssnssmtvnlcNPGISPS 410 Yamadazyma tenu...
EJT73311     501 DFGITMTFLGPDrPVRVGEEFCWSVFVVNRSGTGtapvvsglaistgapqhhqpsplptatpttpavggsaRKLALRVVP 580 Gaeumannomyces ...
XP_004197047 452 LSGLKLTFQGKL-TLKLGEITTWKLQAINNSTYR-------------------------------------LNLSLLAQN 493 Pichia farinosa...
XP_001387446 447 LTGLKLNFKGKL-NIKLGEVNNWKIQAINNSPSR-------------------------------------LNLSLLVQN 488 Scheffersomyces...
EGW35356     442 LSGLKLSFIGKL-NIKLGEVITWKIQAINHASTR-------------------------------------LNLSLLVQN 483 Spathaspora pas...
EMG47784     422 LSGLKLTFIGKL-DLKLGEIVNWKIQAINNSMNR-------------------------------------LNLSLLVQN 463 Candida maltosa...
EER31758     430 LSGLKLTFIGKL-DIKLGEVVNWKIQAINNSPNR-------------------------------------LNLSLLVQN 471 Candida tropica...
CCE40745     410 LAGLRLTFIGRL-DMKQGVATKWKMQAINNSVNR-------------------------------------LNLSLLVQN 451 Candida parapsi...
EDK41417     452 LSGLKLTFIGSF-TLKLGEVSSWKIQAINNSSSA-------------------------------------LNLSIVVQD 493 Meyerozyma guil...
Q6BQ76       459 LSGLKLTFQGKL-NLKLGEIVNWNLQAINNSPNK-------------------------------------LNLSLVVQN 500 Debaryomyces ha...
XP_006684632 411 LFGLKLTFNGNL-TVPLGQVTVWKLQAINNSSSN-------------------------------------MMLSLVLQG 452 Yamadazyma tenu...
EJT73311     581 KRRR---ADLR----VTRPPSSHRAShgrqqslmyqrqqqargnGiDSDEVADAVLDENVVHAMQRGSLLDGA---DVVC 650 Gaeumannomyces ...
XP_004197047 494 SSNs--nGQKAfnayASAASSADKTQ------------------------DKFTIYNRSLLFNTYQSMKANMS---GVLT 544 Pichia farinosa...
XP_001387446 489 PINFnpiYNSGt--tSNNVSSSNLLNseav-----------tgqSnAS--NDIIVYNKFQLYSLYNSLKMNTN---GVII 550 Scheffersomyces...
EGW35356     484 PINFnviYS-NsaphHKNASTHSVSNn----------------dN-KAH-NAAIVHDKSNLYSTYNSLKVNNNs--GVVI 542 Spathaspora pas...
EMG47784     464 PINFnpvYNGNna-tTTNFSSSNLLSgsn-------------giAsKRD-SDIIIYNKSQLYSLYNSLKVNGDg-aGVLI 527 Candida maltosa...
EER31758     472 PINFnpvYSGTna-tTNNFSSSNLLHkv--------------gkAnSR--GDVIIYNKSQLNALYNSLKADGEggeGVLI 534 Candida tropica...
CCE40745     452 PINFnpiYSna--apNNNFSSSNLLNn----------------gSaQKG-QDIIIYNKAQLYSLYNTLKVNADa-nGVVI 511 Candida parapsi...
EDK41417     494 PLHw--aANAAqrgpVSNTSSSNVLG--------------------QKSGNDVLVVNKIHLLNEIATMKPRSG---GVVF 548 Meyerozyma guil...
Q6BQ76       501 PINLn--YGQKnantSNNTSSSNLLNs----------------tS--DN-KEILVYNKVQLFSLYHSLKLNTS---GIII 556 Debaryomyces ha...
XP_006684632 453 QKDi-------------SASTPSVSQ------------------QpLRQRDLVPAYMKPALFSLYNSLKLDNN---GVLL 498 Yamadazyma tenu...
EJT73311     651 LSADARVGPLAPGACHVVELRFLALRAGrgpgdaVESVEAVRVVDLVSQEHVDVRELPAIFV 712 Gaeumannomyces tritici R3-111a-1
XP_004197047 545 LDNDIRIGPIDPGNVYETTIRLIGISPG------IYNLDGIKLFDVGSGDGLDFGKLVEVFV 600 Pichia farinosa CBS 7064
XP_001387446 551 LNNDIRIGPLDSNTVFETDIKLIGISKG------IFNLDGIKIFDINSGDGLDFGKLVEVFV 606 Scheffersomyces stipitis CBS 6054
EGW35356     543 LGNDIRIGPLDSNTAFETELQLMGISKG------IFNLDGVKIFDINSGDGLDFGKLIEVFV 598 Spathaspora passalidarum NRRL Y-2...
EMG47784     528 LNNDIRIGPLDPNTVFETEIQLVGISKG------IFNLDGIKVFDINSGDGIDFGKLVEVFV 583 Candida maltosa Xu316
EER31758     535 LNNDIRIGPLDPNTVFETEIQLVGISKG------IFNLDGVKVFDINSGDGLDFGKLLEVFV 590 Candida tropicalis MYA-3404
CCE40745     512 MNNDVRIGPLDPQTSFETTLTLVGNTKG------IFSLDGLKIFDVATCDGLDFGKLIEVFV 567 Candida parapsilosis
EDK41417     549 LENDIRLGPLETNNVMETSIQLMGIARG------IHNLEGIKVFDTNTGDGLDFGKLVQVFV 604 Meyerozyma guilliermondii ATCC 6260
Q6BQ76       557 LNNDIRIGPLEPNSVFETNLNLIGISQG------IFNLDGIKIFDTSSGDGLDFGKLVEVFV 612 Debaryomyces hansenii
XP_006684632 499 LDNDVRIGPLEPNSVFETNVKIIGLSRG------VFNLDGLNIFDLASGDGLDFGKLVEVFV 554 Yamadazyma tenuis ATCC 10573
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