Conserved Protein Domain Family
RCR

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pfam12273: RCR 
Chitin synthesis regulation, resistance to Congo red
RCR proteins are ER membrane proteins that regulate chitin deposition in fungal cell walls. Although chitin, a linear polymer of beta-1,4-linked N-acetylglucosamine, constitutes only 2% of the cell wall it plays a vital role in the overall protection of the cell wall against stress, noxious chemicals and osmotic pressure changes. Congo red is a cell wall-disrupting benzidine-type dye extensively used in many cell wall mutant studies that specifically targets chitin in yeast cells and inhibits growth. RCR proteins render the yeasts resistant to Congo red by diminishing the content of chitin in the cell wall. RCR proteins are probably regulating chitin synthase III interact directly with ubiquitin ligase Rsp5, and the VPEY motif is necessary for this, via interaction with the WW domains of Rsp5.
Statistics
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PSSM-Id: 403477
View PSSM: pfam12273
Aligned: 50 rows
Threshold Bit Score: 33.9252
Threshold Setting Gi: 399163985
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_013949909      36 RWVLAGIAIICALVLLFFCLCISRRNRRRGAQPMYGTGWFsgppaApagrpsgsnkynsnnnayemnny---qsgynqgg 112 Trichoderma...
XP_001647157      34 RWILFALFGLALIVFLISTLLINRRRSQTGRAPIRGTAWL---------------------------------------- 73  Vanderwalto...
EHY59436          45 KWAVIATILAALALYLVGGYLHAKRRMRKGLPPLAYHRWLvsrrqRmafahqhpqyanqfsfyrnqntpygygqgrhtpy 124 Exophiala d...
CCE35144          29 RWVIFFLLLSLVLLYVVGGTLHAKKRIRNGQEPLAYHRFLvkrhfReppanqqwpngqqqgyyyf-----------qqqp 97  Claviceps p...
EPE24210          37 RWSVFFGILFLFLGYMVGGYWHAKKRINKGLKPLAYHRWLlnrqqRarydpayqnpsayyh------------------- 97  Glarea lozo...
EDO03522          44 RWSIFWGLFLLFMLWIIVGYWHAKRRLRKGLKPLAYHRWMlnrrqRaqydpayqnpdvyyn------------------- 104 Sclerotinia...
CCD46611          44 RWSVFWGLFFIFMIWIIGGYWHAKRRLRKGLKPLAYHRWMlnrrqRaqydpayqtpnvyy-------------------- 103 Botrytis ci...
jgi:THITE_132975  43 KWSLFLGIIAVVGLYLLVGYIHAQRRVRKGLPPLAYHRFLvsratLaqvdpryrypqsvtv------------------- 103 Thielavia t...
EQB51775          43 kWSVFLGLTTLFAAFLIIGYMHGKRRLKKGLLPLGYHRWLfsraqLarvdsryawpqatynny---------------rp 107 Colletotric...
XP_018158600      41 kWSVFLGLTTLFAAFLVIGYVHGKRRMKKGLIPLGYHRWLfsraqLarvdsryawptasyntyrp----------eyynt 110 Colletotric...
XP_013949909     113 ysqqqppygegygyTSPPPYGQGPVpnq-----ttGTT---FN-AN-----DGYmtgqpqYG-------------VQQPQ 165 Trichoderma...
XP_001647157      74 ---------------TPPTYRQSQReyngnsgrvvEDFvptYT-EKantedLGH------FNqrgefipneeaeyVAPPV 131 Vanderwalto...
EHY59436         125 gygqgqaypmgaygPPPPAYGE-------------PDYvpaYT-PP-----AGN---------------------KVDPD 164 Exophiala d...
CCE35144          98 qqsgfqqpaypmndMPLPAYDPN--------------RppvYSgPP-----EGG------SK--------------VDPS 138 Claviceps p...
EPE24210          98 qyppqqygmqpyggAPPPVYDPN------------SAMpptYQ-PP-----VGA------TK--------------VDPT 139 Glarea lozo...
EDO03522         105 qfptaaggqygmhpMPPPMYDPN------------APMpptYQ-PP-----AGA------TK--------------VDPS 146 Sclerotinia...
CCD46611         104 nqfppaggqygmhpMPPPMYDPN------------APLpptYQ-PP-----AGA------TK--------------VDPS 145 Botrytis ci...
jgi:THITE_132975 104 hpyapesqyydmhaVPPPVYDPN------------APRppkYE-PP-----AGA------TK--------------IESS 145 Thielavia t...
EQB51775         108 eyyqqqqgyygmqgMPPPTYDPN------------AARppmYA-PP-----DGG------SK--------------VDPS 149 Colletotric...
XP_018158600     111 hpnapaggyygmqgMPPPTYDPN------------AARppvYA-PP-----DGG------SK--------------VDPS 152 Colletotric...
XP_013949909     166 HAYQP------------DGHYQPPAGPPP 182 Trichoderma virens Gv29-8
XP_001647157     132 LDSEMnrq-------pmNGHDQSIPVPPP 153 Vanderwaltozyma polyspora DSM 70294
EHY59436         165 QN------------------YAPPSGPPP 175 Exophiala dermatitidis NIH/UT8656
CCE35144         139 QNRAPtqraq---esneAPEYAPPQGPPP 164 Claviceps purpurea 20.1
EPE24210         140 QWRAeptrrp-aesgeaAPDYSPPPGPPP 167 Glarea lozoyensis ATCC 20868
EDO03522         147 QWGSqptrrpaddggesAPDYEAPPGPPP 175 Sclerotinia sclerotiorum 1980 UF-70
CCD46611         146 QWRSeptrrpaddggesAPDYEAPSGPPP 174 Botrytis cinerea T4
jgi:THITE_132975 146 QQQSPeqsqq---qpegPVEYAPPAGPPP 171 Thielavia terrestris NRRL 8126
EQB51775         150 QEYG----------------ITPPPGPPP 162 Colletotrichum gloeosporioides Cg-14
XP_018158600     153 QEYG----------------VSPPAGPPP 165 Colletotrichum higginsianum IMI 349063
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