Conserved Protein Domain Family
Glyco_hydr_116N

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pfam12215: Glyco_hydr_116N 
beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
Statistics
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PSSM-Id: 403436
View PSSM: pfam12215
Aligned: 130 rows
Threshold Bit Score: 143.182
Threshold Setting Gi: 156383694
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q54D71                  433 GVPIGGIGSGSINRGWKGDFVRWNL---------NNGLVsNEIVDVD-----K-------FSVYINfdnSntssssssss 491  Dic...
Q01UZ8                   45 AFPLGGVCAGSISLGGRGQLRDWEIfnrsdkg---------------------napsyafPAIWVQsgaR---------- 93   Can...
CCW34131                 21 AFPLGGIGTGTISLGGRGDLRDWEIfnhpgkg---------------------fhlpftfFLGYFEsenD---------- 69   Cht...
CDC58670                 40 ALPLGGIGTGTVSLGGRGELRDWEImnvpgkkyst----------------vttgnnapfFAIYTKpekG---------- 93   Bac...
lgeUNICAMP:PACID_30230    1 -MPLGGIDTGCLSIGSDGGLRQWQIhhtgnhqgdLPGSF---------------------FALRVQrgeP---------- 48   Aci...
jgi:Bcav_1712            16 AMPLGGIGTGNVAIGADGGLRQWQLhnignhhgdLPGSL---------------------FAVRVSqweP---------- 64   Beu...
EDO40868                162 ALPLGALGGGSVALAGDGGLRQWQIsgavnht---------------------ahvpnsfFAIRVEqt------------ 208  sta...
XP_004995688            884 tCPLGPCGGGCIALAGDGGLRQWQIlnrichd---------------------ahvpdsfFALYTNdgkG---------- 932  Sal...
XP_001632968            191 AVPLGGLGTGSFEIRADGSFNEWTL---------------ENQSPGGsaklgRgaldlavLGVRVQkq------------ 243  sta...
CBRAS:ASAC_0609          16 GVPMGGLGTGSFEIGPDGRFREWLIfnnrpwagygq-------------pqwfmtpddlvFFLRIEpegQepilrglftg 82   Aci...
Q54D71                  492 snsstpppqfsnnnnnsynnsynnninnngfdnnlkSKQKAVVLYPGKPk---------------SNLnLDVWNWgLKGD 556  Dic...
Q01UZ8                   94 -------------------------------------KPVARVLES-------------------------------RIQ 105  Can...
CCW34131                 70 -------------------------------------KGVARVLES-------------------------------RIP 81   Cht...
CDC58670                 94 -------------------------------------EAQTTLLAG-------------------------------ALY 105  Bac...
lgeUNICAMP:PACID_30230   49 ------------------------------------TMSRTVLLQQARPspparrdvpggraplvTDDlVPQWQEdLLSQ 92   Aci...
jgi:Bcav_1712            65 ------------------------------------PLDEVRVLQAPAApagnt------rtpmvDDDhVPDWQReLAAA 102  Beu...
EDO40868                209 ------------------------------------nSVKAVALQSN--------------------TwYDQTGFkPAAY 232  sta...
XP_004995688            933 -------------------------------------GVDARVLQ----------------------------------L 941  Sal...
XP_001632968            244 ------------------------------------nLTKVTLLRT---------------------------------H 254  sta...
CBRAS:ASAC_0609          83 lwygsae-------------dytyrgcgpwvvaepyHLPWAKGVEG---------------------------------- 115  Aci...
Q54D71                  557 ---------------------NSCYFGLFPRAWTVYEeP---HPDIRLTCKQVSPVIPHNYQESSYPCGVYVWKIEnNNQ 612  Dic...
Q01UZ8                  106 ppyegqnglgtrsapglvrlaGATFTGEFPLARIDFTdA---ALPVKVSLEAFTPFIPHEPDDSGLPVAVLRYRVS-NPG 181  Can...
CCW34131                 82 ppysgasglptgrvpglprlhSNTFRGEYPFAWLHFHdD---LLPVQLSLEAFNPLIPMDVESSSLPVAFFRWRVR-NPT 157  Cht...
CDC58670                106 dqeylhyegrpvnhhglprfkNSSFDAAYPFGQVHLSdK---GIPVEVTVKGFNPLVPGDAEASGIPIAVLCYEVT-NTS 181  Bac...
lgeUNICAMP:PACID_30230   93 hgg----------------vaSSAMSGIYPVARLHQDc----GLGVDIDLTAWNPMVPTDTEISGVPCALFEFRLT-NTA 151  Aci...
jgi:Bcav_1712           103 hg-----------------vtATTISATYPVARVAYDlG---EVPLAIELEATTPLVPLDEAASSLPVAMFTFTLR-NAG 161  Beu...
EDO40868                233 isdhvvpeaskdllktipgvkTITVGAKYPIAEVSYLsD---EVPVQVHLEAFSPCIPLDSKNSGIPVIMFNFSVT-NTT 308  sta...
XP_004995688            942 lkdl-------------phlsAIEIEGKYPVMDVDYTsL---NNGIKASMECMCPCIPLDSKNSGLPVIFFNFTLT-NTT 1004 Sal...
XP_001632968            255 pplgv-----------ypgveGLQYSGAYPFTKLEVKdNr--FFGVKLELFAHGILRARDSENSTIPAVVFTLRVR-NPT 320  sta...
CBRAS:ASAC_0609         116 ----------------------VEMDVRYPRVTLSYRdslldELGVEVSLDAYSPLLPGDLKASSTPAVILRFRVT-NRG 172  Aci...
Q54D71                  613 YSSADVSLMLTWQNSIGT----------------QSDQDGGHFNKYFTFGDNdqqspssssssssgnnkknIKG--ITMI 674  Dic...
Q01UZ8                  182 AAAAKVSIAWSIDSPVG-----------------RTQAADTRTNEYREAGG--------------------LAG--LMMT 222  Can...
CCW34131                158 EHALSATIALCLLNPIGLtghel-------pgsrYHQQLGRNLNTWQEENA--------------------LHG--IFMT 208  Cht...
CDC58670                182 QTPVDVSICGSIRNFIGKdgskfvt---dwkgdyIPVGCKDNRNIYKSAQG--------------------LQG--IYFC 236  Bac...
lgeUNICAMP:PACID_30230  152 DCAAQCWLAGSLHNGVGLdpsat-------phgvRAPGYGSNTNTLEREPGWt----------------------rLVMD 202  Aci...
jgi:Bcav_1712           162 TFPVHGYLAGALQNAVGYdgvtp-------idgtRFRGYGGNTNRVRRRASWt----------------------dLVMT 212  Beu...
EDO40868                309 SSDAQVSLLGSLQNIAGWdglsdigldgptgswiSNPGFGSNLNYTLSKSGF-------------------FEG--IGMF 367  sta...
XP_004995688           1005 SDAVDVSLLMSQHNLVGWdgeta------ienetEFAGYGGNVNAKMTMDG--------------------VTA--VDMT 1056 Sal...
XP_001632968            321 ADSVNVSLLLNLP--LGEq--------------qDTERKFSNLTSWFHENVTsskecawlchqatrcmawsLVEdrCVMM 384  sta...
CBRAS:ASAC_0609         173 TSRVKVSLMAVARNL--------------------ARSAGGRPSSEVREGPDwt---------------------aMIMS 211  Aci...
Q54D71                  675 HKKEQKCtlpng------------ntkvF------NDPMEYSIA-----------VM--------DEP-----D---VEI 709  Dic...
Q01UZ8                  223 NAELPAA---------------------D------ALKGSFALA-----------AMgtagarvtHLRgweqgRw-wNSP 263  Can...
CCW34131                209 GGKPQPS---------------------D------PHHGDITLMtdwy--------------nvsYRLrweraGw-wDDA 246  Cht...
CDC58670                237 SEGVEKT---------------------D------PAYGNMTLV-----------TNape--gvtYRTsskadDw-sNGI 275  Bac...
lgeUNICAMP:PACID_30230  203 SHGCDRR---------------------H------DSAGQMALL-----------CEgq----adALSqfqepSelfHFL 240  Aci...
jgi:Bcav_1712           213 NDSLPPE---------------------H------PGAGSMALA-----------CDdpr---asALPaysgtEellAFL 251  Beu...
EDO40868                368 KPKLGDSk--------------------L------NENGHVAIAvvpndrdkqidRVlqy---qsVKD------------ 406  sta...
XP_004995688           1057 STQFRDT---------------------D------PQAGHIAIAaltgd-ssevsTMlqy---daHKT------------ 1093 Sal...
XP_001632968            385 SNMPPHAykrgvssgvkgtwhktkdsltCerpgiyPNSGSTTIG-----------AMhhg---sgYEPtaqpiKgqrSSF 450  sta...
CBRAS:ASAC_0609         212 ACCVDERh--------------------P------MARGALAVAslgqqssgavgSVdss---nrPQLvnllrR------ 256  Aci...
Q54D71                  710 SFcerfettsrlDAAN-----------LWYTF-NK---KGEL--------ENNKDSRPSAPKKP-I----GAAVA-AKVK 760  Dic...
Q01UZ8                  264 ML-------------------------YWDDF-SA---DGEL-------------------GPEaAkmnaVGALC-VQRT 294  Can...
CCW34131                247 QN-------------------------FWDDFvSS---KGHL-------------SSDPQASPSpDhqtdVATLA-LHAH 284  Cht...
CDC58670                276 LD-------------------------FWDDF-SA---DGEL----------------TEMSKQ-YdadpMASLA-VKKK 308  Bac...
lgeUNICAMP:PACID_30230  241 DRl-----------------------pGWEH-------PGPVaalpglpsEAPGAGGSWPGPSP-AgqtwLGALS-RKVA 288  Aci...
jgi:Bcav_1712           252 RS------------RSmggdtarftgaPWAADsHA---EGPG---------------AARGPSP-AaetwLGALA-SRFH 299  Beu...
EDO40868                407 ---------------------------LWSNF-QKlqfDGSV----------------APQLSP-EgktwNGAMC-STRN 440  sta...
XP_004995688           1094 ---------------------------LWSHF-AK---TGKL----------PGQGQSTP--SP-SgktwNGAVC-VSRT 1128 Sal...
XP_001632968            451 GVs--------dDFTS-----------LWSLF-KD---TGQL------------------DARH-SygihGSGV--VTKA 486  sta...
CBRAS:ASAC_0609         257 ---------------------------LLVDF-RG---DGTL---------------TGPSSMT-SdldvFSAVAtRPIE 289  Aci...
Q54D71                  761 VAA----GSSKTIVFCISWDAPITR---FQMGSA------------YY-KRYTKFYgnqggNSQRIAYDAINNWKHW 817  Dictyo...
Q01UZ8                  295 IAA----GGHADFPFLLAWHFPNRTprrIGWSSTkge------edtLIgNHYCARFa----DAWAAAEYAAANLERL 357  Candid...
CCW34131                285 LAP----KSHVELPFLIAWRFPNLV---NYWNSEpev------hgkHIgNHYATRFq----SSWEAARYTFENLASL 344  Chthon...
CDC58670                309 LAP----GETRSFTFFLTWNFPNR----KAWSSS------------VIgNYYSQQYp----DSWETARKIIPQMPEL 361  Bacter...
lgeUNICAMP:PACID_30230  289 LGPagskDASATVRFAVCWSFPNRY---VTWPDFgpdrpeygatrfWLgCHYTKAWp----DARHVADAVTRMWD-L 357  Acidip...
jgi:Bcav_1712           300 VEP----GESTTVRFLMAWHFPNRY---VNFTQFgperpewgptrfWIgNHYTLAHd----DAAAVIADVTSRWDEL 365  Beuten...
EDO40868                441 IAA----NATETFTFLLAWHFPHRY---VNWSQSgfgind-pnsdyYIgTKYSEYWk----NIEEVLFYSTSNLKDL 505  starle...
XP_004995688           1129 LAA----GATTTITFVLAWHFPNRY---VNWSQKgfyidd-tksqyWLgNAYANFWp----SITEVLEYAKNtgeal 1193 Salpin...
XP_001632968            487 LGP----RQNGTLSLVMAWYYPFRD---HSGE--------------LVgSYYSNIFt----SSESVAEYVTRNYKQm 538  starle...
CBRAS:ASAC_0609         290 LTP----GSSEELYWAIAWHFPNHI---DASGQ-------------RVgHYYENFFg----NVEQVISYIFENKHEL 342  Acidil...
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