Conserved Protein Domain Family
NotI
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pfam12183: NotI 
Restriction endonuclease NotI
This family of proteins is found in bacteria. Proteins in this family are typically between 270 and 341 amino acids in length. There is a conserved CPF sequence motif. The type IIP restriction enzyme, NotI, is a homodimer that recognises the 8 bp DNA sequence 5'-GC/GGCCGC-3' and cleaves both strands of DNA to create 5', 4 base cohesive overhangs.
Links
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Statistics
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PSSM-Id: 492432
Aligned: 3 rows
Threshold Bit Score: 347.377
Created: 16-Feb-2025
Updated: 28-Apr-2025
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Q28NA7         66 NVLRSKL-------AQQQAEELpCPFrtDSPHPTCTKPG-----GVCSIriyreeagviapidGERGRLRALCPWRFHQD 133 Jannaschia sp....
jgi:Caur_2525  15 EVFGYQItdqseaaARCRSARL-CPF--QGQDRKCTKDKannplGVCAI--------------YHNNEPVITCPIRFRQN 77 
Q5LUK6         66 SVLRDKL-------TRQQAEEIpCPFrpDTPNATCTKPG-----GVCSIrvyrgeknrvepitGERGRLRALCPWRFHQD 133 Ruegeria pomeroyi

Q28NA7        134 GTAFDKIGESLLAdpsplrAGEVGFLESTGNLDSAAGEDVGRIDMILVKSNSvDGAPMDWVAVEVQAVYFSGKKMSIEFD 213 Jannaschia sp....
jgi:Caur_2525  78 WLIAQDAALFFFG------EGTRWSILTEIRLPDAFGKSAGNIDVVLVSYDD-EGRITDFGAIEIQAVYISGNVRSFFEH 150
Q5LUK6        134 GKAFSEVGKRLLNdpdpikAGEVGFLESSGNLDSDPGEDVGRIDMILVKSNGvEGAPMDWVAVEVQAVYFSGKKMSIEFD 213 Ruegeria pomeroyi

Q28NA7        214 HLKLTQGRLSMAQEKRR----PDYRSSGVKRLMPQLLTKVPTLRRWGKKMAVVVDAPFFYSMGKMERVPhLSNADIVWFL 289 Jannaschia sp....
jgi:Caur_2525 151 YMRDPQGYIVGDWIGETpvprPDYLSSSRKRLVPQLMYKGAILRAWNKKMAVVVDEQFFQTLPQLASIP-PQDANMAWFI 229
Q5LUK6        214 HLIKTQGKISMAREKRR----PDYRSSGVKRLMPQLQTKVPTLRRWGKKMAVVVDAPFFYSMGEMARERdVSNADIIWFL 289 Ruegeria pomeroyi

Q28NA7        290 VDFKQAAP----GAPFQL 303 Jannaschia sp. CCS1
jgi:Caur_2525 230 YRLMPGRQahegTERYYL 247
Q5LUK6        290 ADFKEDLN----GGGFKL 303 Ruegeria pomeroyi
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