6HUM


Conserved Protein Domain Family
NdhO

?
pfam11910: NdhO 
Click on image for an interactive view with Cn3D
Cyanobacterial and plant NDH-1 subunit O
The proton-pumping NADH:ubiquinone oxidoreductase catalyzes the electron transfer from NADH to ubiquinone linked with proton translocation across the membrane. It is the largest, most complex and least understood of the respiratory chain enzymes and is referred to as Complex I. The subunit composition of the enzyme varies between groups of organisms. Complex I originating from mammalian mitochondria contains 45 different proteins, whereas in bacteria, the corresponding complex NDH-1 consists of 14 different polypeptides. homologs of these 14 proteins are found among subunits of the mitochondrial complex I, and therefore bacterial NDH-1 might be considered a model proton-pumping NADH dehydrogenase with a minimal set of subunits. Escherichia coli NDH-1 readily disintegrates into 3 subcomplexes: a water-soluble NADH dehydrogenase fragment (NuoE, -F, and -G),the connecting fragment (NuoB, -C, -D, and -I), and the membrane fragment (NuoA, -H, -J, -K, -L, -M, -N). In cyanobacteria and their descendants, the chloroplasts of green plants, the subunit composition of NDH-1 remains obscure. The genes for eleven subunits NdhA-NdhK, homologous to the NuoA-NuoD and NuoH-NuoN of the E. coli complex, have been found in the genome of Synechocystis sp. PCC 6803 which has a family of 6 ndhD genes and a family of 3 ndhF genes. Two reported multisubunit complexes, NDH-1L and NDH-1M, represent distinct NDH-1 complexes in the thylakoid membrane of Synechocystis 6803 -cyanobacterium. NDH-1L was shown to be essential for photoheterotrophic cell growth, whereas expression of NDH-1M was a prerequisite for CO2 uptake and played an important role in growth of cells at low CO2. Here we report the subunit composition of these two complexes. Fifteen proteins were discovered in NDH-1L including NdhL, a new component of the membrane fragment, and Ssl1690 (designated as NdhO), a novel peripheral subunit. The three nuclear-encoded subunits NdhM,NdhN and NdhO are vital for the functional integrity of the plastidial complex.
Statistics
?
PSSM-Id: 403201
View PSSM: pfam11910
Aligned: 34 rows
Threshold Bit Score: 77.2837
Threshold Setting Gi: 474068742
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
6HUM_O           3 IKKGDLVKVVAEKLANSLEALaSDHRYP-----PYLFEGRGEVVDI---------RGDYAQ-------IKF-PVPTPTVW 60  Thermosynecho...
EMS54216        49 MKKGQIVRVEKEKYLNSINYL-SVGHPPfykglDYIYEDRGEAINFadprlynisTAGFSLliaklssIAWvGIPTPPAW 127 Triticum urartu
Q9S829          75 MKKGQIVRVEKEKYLNSINYL-SVGHPPfykglDYIYEDRGEVLDLrv-----feTGEYAL-------VGWvGIPTAPAW 141 thale cress
Q46HQ2          21 LRKGDLVKVDREKYSNSLESKaSDTNLP-----EYIFQGPGEVLLI---------KGDYCQ-------VRW-RRPVPDVW 78  Prochlorococc...
jgi:Cyagr_2936  12 LKKGALVRVNRAAYTNSLEAGaSDPVPP-----GYLFEGPGEILAI---------QGEHAQ-------LRW-RLPVPDVW 69  Cyanobium gra...
A5GQL3           5 LKKGSLIRVNREAYLSSVEAKaSAGDPP-----AYLLEGPGEVLAV---------KGDYAQ-------LRF-RQPVPDIW 62  Synechococcus...
A5GNZ2          18 LKKGALVRVNRAAYEGSVEAGaSDPHPP-----AYIFEGPGELLVV---------KGTYGQ-------VRW-RRPVPDVW 75  Synechococcus...
Q7VE72          16 LKKGSLVRVNPEAYKNSLESLaSDQSSP-----EYIFEGPGELVAI---------KQDYGQ-------VRW-RRPVPDVW 73  Prochlorococc...
Q7V4H6          14 LKKGALVRVNRHAFSSSTEAAaSDPSPP-----DYIFEGPGELLAV---------KEGYGQ-------VRW-RMPVPDVW 71  Prochlorococc...
Q8GIT3           5 LKKGSLVRAIAEQLQGSVELLaSDGRIP-----SYVLETNGEILDI---------KGDYAL-------VRF-SRPTPNVW 62  Synechococcus...
6HUM_O          61 LRLDQLE 67  Thermosynechococcus elongatus BP-1
EMS54216       128 LPTYMLI 134 Triticum urartu
Q9S829         142 LPTDMLI 148 thale cress
Q46HQ2          79 INSDHIV 85  Prochlorococcus marinus str. NATL2A
jgi:Cyagr_2936  70 LRLDQLE 76  Cyanobium gracile PCC 6307
A5GQL3          63 LRMDQLE 69  Synechococcus sp. RCC307
A5GNZ2          76 LRMDQLE 82  Synechococcus sp. WH 7803
Q7VE72          74 LRLDQLQ 80  Prochlorococcus marinus subsp. marinus str. CCMP1375
Q7V4H6          72 LRIDQLE 78  Prochlorococcus marinus str. MIT 9313
Q8GIT3          63 LRLDQLQ 69  Synechococcus elongatus PCC 7942 = FACHB-805
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap