6ICZ


Conserved Protein Domain Family
Slu7

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pfam11708: Slu7 
Pre-mRNA splicing Prp18-interacting factor
The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.
Statistics
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PSSM-Id: 403036
View PSSM: pfam11708
Aligned: 105 rows
Threshold Bit Score: 100.03
Threshold Setting Gi: 240131130
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6ICZ_Z       161 FDYDGKRDRWNGYNPEEHM-KIVEEYAKVDLAKRTLKAQKLQEELAsgklveqanspkhqwgEEE----PNSQMekdhns 235
CCG81311     140 TTWDSKRDRANGYDFDNHAaILKASFDRVEALRAERGQHDGV---------------------------VEDDEsnpqsv 192 Taphrina deform...
EFX04902     167 LGWDAKRDRWLGFDARDYA-SMVAGREKMNEARRRLGGERG----------------------------GGGAEggeegg 217 Grosmannia clav...
EKG11631     124 LGWDAKRDRWNGYDASEYS-AVIEEYNEIEEMRKKLKGEDLK--EG----------------DEEgd------------- 171 Macrophomina ph...
Q0V5M5       117 LGFDAKRDRWNGYDASNFD-EVIDEYKALEEIRKKAKDAEKG--DD----------------KSDee------------- 164 Parastagonospor...
XP_007781546 121 LGWDAKRDRWNGYEAQEYE-KVVQEYNELEDMKREMKSGKDGQKRL----------------TEDgeedGD--------- 174 Coniosporium ap...
XP_011271900 122 LDYDGQRDRWYGYDSKDYK-KYLTNWE----IKQKEKENEAD-------------------------------------- 158 Wickerhamomyces...
EER30691      79 EDYDSKRDRWYGYSTEEWL-THLKNWKQTKQPDQEIENNDSD-------------------------------------- 119 Candida tropica...
Q6BMK7        84 EDYDSKRDRWHGYEAQEWD-KIAENWDKIKKKKQKTKNASVE---------------------------EDSD------- 128 Debaryomyces ha...
Q9Y7Y2       153 VSWDAKRDRWNGYDATDYK-KVIERYEKLDELQNKGEENRDA---------------------------SENSAvkasrn 204 Schizosaccharom...
6ICZ_Z       236 ED----ED----EDKY-ADDIDMPGq--nF--D-----SKRRITVRNLRIREDIAKYLRNLDPNS-----AYYDPKTRAM 292
CCG81311     193 AD------------------------------------QTKSTSNSHMRIREDTASYLAK-DREN-----DSYDPKTRAM 230 Taphrina deform...
EFX04902     218 SSsktaAEgdknDTRY-EEDADMGV--------------SRTNGAADLRMREDTARYLVNLDPDS-----AKYDPKRRKV 277 Grosmannia clav...
EKG11631     172 ----vdEE----GAKY-EAETDMGRk--------------QPTSTRNLRIREDTAKYLLNLDLDS-----AKYDPKTRSM 223 Macrophomina ph...
Q0V5M5       165 -----edE----GDKY-DAETDMGRk--------------QATSTRNLRLREDTAKYLLNLDLDS-----AKYDPKTRSM 215 Parastagonospor...
XP_007781546 175 -------E----GAKY-EEETDMGRq--------------QATSTRNLRLREDTAKYLLNLDLDS-----AKYDPKTRSM 223 Coniosporium ap...
XP_011271900 159 ------------NTKQyDTDEEIELkelgLdhDlefinTEKQPGEKTIRIREDKAIYLQNYTNDN-----ITYDPKSRMI 221 Wickerhamomyces...
EER30691     120 -----------------DTDYELELielgLdkKdlnknIKEDPMEKMLRDRQDIPAYIYNITSNPnnkirIDYDPKSRLA 182 Candida tropica...
Q6BMK7       129 -----------------DTDYELELvelgLdsKdiknnLKEDPLEKTIRDRQDVPAYILNITSSNk----IHYDPKSRLT 187 Debaryomyces ha...
Q9Y7Y2       205 STvsgsED-------------------------------SASITTPSLRMREDVVAYLRADNKN------LQYEPKSRSM 247 Schizosaccharom...
6ICZ_Z       293 REN-PYANagKNPDE--VSYAGDN-FV-RYTGDTISMAQTQLFAWE-AYDK--------------GS------------- 339
CCG81311     231 KDAvPSAGtgIDKKD----------FT-RGSGDAAAFTKLQAFAWN-EQAT--------------GSttdeqkkkigqvp 284 Taphrina deform...
EFX04902     278 VDGgAFLDesARL------FAEQN-FA-RASGEAAAFARDERYAWElHDQA--------------GDa------------ 323 Grosmannia clav...
EKG11631     224 VDS-GAT-----ADKsaALVAEEG-FM-RASGDAAEFERAQKLAWE-SQQR--------------GDr------------ 268 Macrophomina ph...
Q0V5M5       216 VDS-GVT-----SDNasKLVAEEG-FM-KASGDAAEFERAQRYAWE-TQER--------------GDk------------ 260 Parastagonospor...
XP_007781546 224 VDS-GAT-----SDNaaALVAEEG-FM-RASGDAAEFEKAQRYAWE-TQER--------------GDk------------ 268 Coniosporium ap...
XP_011271900 222 RDQ-St-----------GFFNEQNlFVkHMTGDALKYEKTKTFAWD-EEKK--------------GV------------- 261 Wickerhamomyces...
EER30691     183 KDMs------------kGFLNDRSqFVkKLTGAATEFTDLQKIASGlDNEHeqerkqarlkqqffGDdsqfkne--vpet 248 Candida tropica...
Q6BMK7       188 KDPs------------kGFINDKNqFVkKLTGEAKRLDNLQKFAWEqNRQQeemkqreafeqkltGKkhseggp-deyqv 254 Debaryomyces ha...
Q9Y7Y2       248 RDEtGYHMvdDSS--------GGAgFVkASGGEKEDFEKLQMFAWE-AERS--------------GT------------- 291 Schizosaccharom...
6ICZ_Z       340 --EV-HLQADPTKLELLYKSFKVKKEDFKEQQKE-------SILEKYGGQE-HLDAPPAEL-LL-AQTEDYVEYSRHGTV 406
CCG81311     285 fgDV-HAQATPSHAALLYK----ERLAAKAAEKA-------GILDRYGNDT---QALPSHLlDV-ANDEKV--YSKEGLL 346 Taphrina deform...
EFX04902     324 --GL-HLQANPTAAAHRRRVEADVEAEKRATMAA-------LMRDKYGVDE-----TPAAVpSItAASEHFVEYDETGQV 388 Grosmannia clav...
EKG11631     269 -nQL-HLQANPTSGEFFRKKEQEEAEAKRAAKKK-------ELLEKYGGSEhLKDAPMKDA-AI-IENEKFVEYDEKGRi 337 Macrophomina ph...
Q0V5M5       261 -nKL-HLQANPTSGEIMRKKELKEADEQKAAKAK-------ALAEKYGTQEkFTDDTLRKT-AV-TESEKYVEYDERGRi 329 Parastagonospor...
XP_007781546 269 -nKV-HLQANPTSGEYFRKKAAKEAEERKEQQRK-------ALAEKYGDQAlVQNKALRDA-AI-LESERYVEYDETGGv 337 Coniosporium ap...
XP_011271900 262 --DAsNYASNPTLADIKINETKNENRIKSEKVKS-------ELMKKYDGSL-SIKDQSDEE----EESEPEPQYk----- 322 Wickerhamomyces...
EER30691     249 dlNL-SLEASPTLMMLKAKQLQKEKLKLKDKEKEkpnntknEISQKY------------------ASSGITTNLDKn--- 306 Candida tropica...
Q6BMK7       255 dlNL-NMEANPTAMMLQARHKQEQQQASHDQKKS-------DLVAKYGGGE------------FlNKSKEFVNVTESIQT 314 Debaryomyces ha...
Q9Y7Y2       292 --RV-HVVANPTAGELEFRKNKASRMTTQKHIDQ-------SILDRYGDGTs--KVKDKKAkNNeKEVPDLAILDKs--- 356 Schizosaccharom...
6ICZ_Z       407 i-----KgqerAVACSKYEEDVKIHNHTHIWGS 434
CCG81311     347 lt-------pkHVVRSRYNEDVLLNGHTSVWGS 372 Taphrina deformans PYCC 5710
EFX04902     389 kerl----rapRADRSKYAEDVLPMNHTAVWGS 417 Grosmannia clavigera kw1407
EKG11631     338 ------KglpkTKAKSKYPEDVFINNHTSVWGS 364 Macrophomina phaseolina MS6
Q0V5M5       330 ------KgapkVKAKSQYPEDVHLNNHTSVWGS 356 Parastagonospora nodorum SN15
XP_007781546 338 ------KgapkTKPKSKYPEDIFIHNHTSVWGS 364 Coniosporium apollinis CBS 100218
XP_011271900 323 -----------DFTISKYIEDEYINNHTSVWGS 344 Wickerhamomyces ciferrii
EER30691     307 -----------GLKKTRYPEDQLTGNHKFIYGS 328 Candida tropicalis MYA-3404
Q6BMK7       315 gdkpinKdk-nGLKRSIYPEDNYSMNHQSIWGS 346 Debaryomyces hansenii CBS767
Q9Y7Y2       357 -----------EKSKGESNTDEESENLVIVEGD 378 Schizosaccharomyces pombe 972h-
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