7S0K


Conserved Protein Domain Family
HAP2-GCS1
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pfam10699: HAP2-GCS1 (This model is not part of the current CDD release)
Male gamete fusion factor
The gene encoding Arabidopsis HAP2 is allelic with GCS1 (Generative cell-specific protein 1). HAP2 is expressed only in the haploid sperm and is required for efficient guidance of the pollen tube to the ovules. In Arabidopsis the protein is a predicted membrane protein with an N-terminal secretion signal, a single transmembrane domain and a C-terminal histidine-rich domain. HAP2-GCS1 is found from plants to lower eukaryotes and is necessary for the fusion of the gametes in fertilisation. Studies in the green alga Chlamydomonas and the malaria organism Plasmodium showed that it is involved in a novel mechanism for gamete fusion where a first species-specific protein binds male and female gamete membranes together after which a second, broadly conserved protein, either directly or indirectly, causes fusion of the two membranes together. The broadly conserved protein is represented by this HAP2-GCS1 domain, conserved from plants to lower eukaryotes. In Plasmodium berghei the protein is expressed only in male gametocytes and gametes, having a male-specific function during the interaction with female gametes, and being indispensable for parasite fertilisation. The gene in plants and eukaryotes might well have originated from acquisition of plastids from red algae.
Links
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Statistics
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PSSM-Id: 519504
Aligned: 12 rows
Threshold Bit Score: 219.656
Created: 15-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
7S0K_A          1 ASVGGSLTGVGSI----------VTCLDSGRPGSIPCSKKWVLTLAVENGATAAsssvsa--------tqavygSSSANA 62   Cyanidioschyz...
XP_020431419   38 YLKCQKMFVVSLT----------IDSNQDHTELSQFTVNDVKNENGDTFSLVYP--------------------VEISFS 87  
CEM12681       39 DLDCTEKLVLLLN----------INTGEGGSEYLELGGVSVEDTLGDGERVNLSfs----------------kdIRVKIF 92  
A0BZ59         20 NTECSEKMLISLT----------VENAQNTVTEYIKISETTIDNQTSQLKTP----------------------IIISIT 67   Paramecium te...
ODN05384       75 FSTCRKKLLITLR----------ITESTRNFVPTALILIDRAKNTLTQKPSSFGr------------------pIAVKFH 126 
ERL89993      106 LRSCEKKLSLTLK----------VKNRGLTNCQPQYIVLDHVFDPATNKKQKLLn------------------pYVLKVT 157 
EFJ27200       41 AIQCKKKILVTVA----------IPSGDGGNGEALIAEVKDPTSRDGKQVLEKS--------------------ISVNIA 90  
EXC34107       56 NLNCNKKIVLNLA----------VPSGSSGGEASIVAEIVEVEENSTQKMQTLRip------------------------ 101 
1_pfamImport    1 SLNCTAKIVLNMA----------VPSGSSGGEASIVAEIVEVEENSTSNNMQTLri-----------------pPVITVN 53  
XP_004989263   53 GGTCKKKLVMTLS----------IGNGQQLPEVVEVTNYIENGQEKPLGQR-----------------------YRIYPT 99  
KJE94895       27 SVAPQSGVVLDTSptnnnaftitFSHNVSMMSGGVLTPLSSLSSSNLNQIFIVAgskpislarnsfvsfpsavsLNLAAP 106 
XP_004341525   53 TISCDRRMIVTLT----------VDSGQNNTEQLELVLDSTQDEDGVLRTLEHP--------------------VQIQWA 102 

7S0K_A         63 TVRSADNPNTV------YAFKYQVHITltksrirldyply-------------------yqsdfnnkpyeiVYKYNQKgp 117  Cyanidioschyz...
XP_020431419   88 KSKQYGKSSLI------YRKSYSEEKYenvhytndyl-------------------------lfssckdspSDHTCPT-- 134 
CEM12681       93 KEDVLVSYPLV------YIQDVYDDAAevqnfdnrravggifsstdncetdemklyrkhtedntwvkdnkwNRLSCGVlf 166 
A0BZ59         68 KTPVYAFYPLK------YTEDYNSQPYevkiagail---------------------------scddswysNSPTCGF-- 112  Paramecium te...
ODN05384      127 QEKLFQKYHLD------YLYHVNGKMWeevinsenskn-----------------------yaacnnslqtSKPTCGF-- 175 
ERL89993      158 QKPVYQVYDLV------FENVVNSGPQekvlnknne--------------------------rfsgcstdsTSATCGT-- 203 
EFJ27200       91 KTDSIVKYALE------YLKNVAGDLNervikkkk-----------------------------gcntklnDKATCGV-- 133 
EXC34107      102 ----------------pVLTDVSYKPEeyyvk----------------------------------trkcePEASAKVvk 131 
1_pfamImport   54 KSATYALYELT------YIRDVAYKPQeyyvk----------------------------------trkceRDAGTNVvq 93  
XP_004989263  100 KSDAFVNYPLV------YQSSINSLPYehwfkvkrsntqi------------------afffdpcrdaptaEHPTCGY-- 153 
KJE94895      107 GRSLIVSFRLSisasdyYADLMLADVF--------------------------------------------YLTKSGKsl 142 
XP_004341525  103 KTIPRLLYPIT------YVGRTNNKPYetitykddilf----------------------lfdecndspssSSPTCGW-- 152 

7S0K_A        118 l--------nwLDNQCVATWGSSDPTCGYAYnpswstkpadril------------------------------------ 153  Cyanidioschyz...
XP_020431419  135 -----------VRDASGNQVPYSQGFCCSCDlgsyvgidrdsr------------------------------------- 166 
CEM12681      167 kde----gepdGPDRKYEPIPSSGGFCCFCDnfdaafptiewk------------------------------------- 205 
A0BZ59        113 ------------QYEKKEKIFDSQGFCCSCGildliglsdefa------------------------------------- 143  Paramecium te...
ODN05384      176 ----------------IHGTSEVTGFCCSCKdhkgnvqqrsgh------------------------------------- 202 
ERL89993      204 ------------LQYENQEVPYSTGFCCSCQsprsskdptdiagslplddklkhvdqkeisemalanfdpakpaenpllg 271 
EFJ27200      134 -----------LGDSKGNVVPGSSGFCCTCKplkqikhfrgm-------------------------------------- 164 
EXC34107      132 i-------cerLRDENGHIMEHTEPICCPCGpqrrmpsscg--------------------------------------- 165 
1_pfamImport   94 icerrvillllLRDEKGHIIEHTEPTCCPCGpqrrvpsscg--------------------------------------- 134 
XP_004989263  154 ------------FVRKGERIPNSQGFCCKCKffsgggnnayr-------------------------------------- 183 
KJE94895      143 pfsspatyrlqNVAPDTCLANIASPVTSLCS------------------------------------------------- 173 
XP_004341525  153 -----------FYNADGTVVRDSQGFCCSCDlsevlwlsneqtr------------------------------------ 185 

7S0K_A        154 --------------------------------------------ysqgfccdcnagdLLGLSPNRIRGGLDCSLLNF-DN 188  Cyanidioschyz...
XP_020431419  167 ----------------------------------------------shlsctlfggrSSSASCMAQRPLLYDSYSIE-PP 199 
CEM12681      206 ---------------------------------------------rglqlecwtfnsPWSCHAMRYGEEKSEIYRVQ-TH 239 
A0BZ59        144 ---------------------------------------------rgnichkaglttATMAFCLRYSTLWYSAYEIS-TY 177  Paramecium te...
ODN05384      203 ----------------------------------------------rcdvpgktpnlLTSAHCLRSSPFTYNVYKMS-PP 235 
ERL89993      272 sdlsnaenliggrfnlprigrhlvkdkkeghksrlhrrggqdcnddytppnvdpetyHDSAHCMEFSDVWYSMYKIV-HP 350 
EFJ27200      165 -----------------------------------------------pkpghcgisdAGYAFCLRFGQMWCVMFRIR-TG 196 
EXC34107      166 -----------------------------------------------svfdkwmkgkPNTAHCLRFPGDWFHVFSIG-QR 197 
1_pfamImport  135 -----------------------------------------------nffdklmkgkANTAHCVRFPGDWFHVFGIG-QR 166 
XP_004989263  184 ----------------------------------------------gnlkcrlysphYSSAHCLRFWPNWYDAWELD-RA 216 
KJE94895      174 ---------------------------------------------------------FVDASSVPSTPVSSPTWSGTiNE 196 
XP_004341525  186 --------------------------------------------agltcslfafgvdGSSAHCLRFDQLWYDVFSIG-AA 220 

7S0K_A        189 PTESAHCLRFDSLWYsafqigepd-------------------------------------------------------- 212  Cyanidioschyz...
XP_020431419  200 VTTYDIVVNITQFNV----------------------------------------------------------------- 214 
CEM12681      240 KWQWSISITMYDIAAmymceeilggvsgyeakshlccpkacgecgndecdkapggatnccaapmlssnrncsshappcvv 319 
A0BZ59        178 SIYYNITISITYSNQ----------------------------------------------------------------- 192  Paramecium te...
ODN05384      236 TIYQKVSFEVFKSKQnln-------------------------------------------------------------- 253 
ERL89993      351 EISHSLEIKIFEKVGmgg-------------------------------------------------------------- 368 
EFJ27200      197 TISFEITITLTDQNGn---------------------------------------------------------------- 212 
EXC34107      198 SLGFSVKIQVKTGSE----------------------------------------------------------------- 212 
1_pfamImport  167 SIGFSIRIEVKTRYK----------------------------------------------------------------- 181 
XP_004989263  217 QVSHTIRLAVYKETSfngtipeptqac----------------------------------------------------- 243 
KJE94895      197 ELAVNISVNIQNSGSassslgaiklthqdveyellsdwvvsiqrndtkfvvptipntqryydamevsrgrianstylagl 276 
XP_004341525  221 QVSFEVVLSVKKYQTmtdm------------------------------------------------------------- 239 

7S0K_A        213 ----vnfvilvnVTKCPLANSTIK---------------------------------------SISGLVGNQDqaIQNCS 249  Cyanidioschyz...
XP_020431419  215 ------------TTGASQTQTYRL---------------------------------------GNDNLILNAN--GIVIK 241 
CEM12681      320 sfgdsgpshsssSFREPTAWTATI---------------------------------------NPNRKVAEAQ--SIIVK 358 
A0BZ59        193 -----------------EQEELQL---------------------------------------GSEVKVVQGK--TLIGR 214  Paramecium te...
ODN05384      254 ---------gtiSWEQLSHTKFGV---------------------------------------TVDSDNLVNVsgDVKVS 285 
ERL89993      369 ---------sriSWRRVSSTPIVL---------------------------------------GTANPRYANLegTILAY 400 
EFJ27200      213 -----------kASSRIIGFVLRL------------------------------------------TLLAEKPdgNWQQF 239 
EXC34107      213 ------------------ISEVFV---------------------------------------GPENRTVASNdnFLRVN 235 
1_pfamImport  182 ------------------VSEVIV---------------------------------------GPENRTATSKdnFLRVN 204 
XP_004989263  244 kdsnarptrrvnGFAFYCVDVLLI---------------------------------------GVQNKVARSTngTVIAT 284 
KJE94895      277 vdtsvssatvqtFLNAASAGSLTLivlnlapeqawdyvdswtttsqyesglstaqlrfanlsaGTSTFINFNSvwTIRQE 356 
XP_004341525  240 ---------ygnTTGGYVTETLRL---------------------------------------SPSQTTGTAAggDIFAK 271 

7S0K_A        250 TEII-------------------------------------SLSPSSPIG-YASNGKIS--------AQaigdfa----- 278  Cyanidioschyz...
XP_020431419  242 LVGD-------------------------------------FASPQALRT-FEDSMLFVnne--qsdPN----------- 270 
CEM12681      359 YQGS-------------------------------------LLPDDGVPTwLEDKYVVW--------PAed--------- 384 
A0BZ59        215 IIGD-------------------------------------FTPLNPPPS-LESFYFMR--------PSsp--------- 239  Paramecium te...
ODN05384      286 YRAHse----------------------------------gIAASATIFD-ASDQRLLIplpnpfvkPEn---------- 320 
ERL89993      401 YSVNe------------------------------------QIDSEFALD-CKSHFLLI--------PEvagrn------ 429 
EFJ27200      240 TRGS---------------------------------------------------------------PR----------- 245 
EXC34107      236 LIGD-------------------------------------FVGYTNIPS-FEDFYLVI--------PR----------- 258 
1_pfamImport  205 LIGD-------------------------------------FVGYSSLPS-FEDFYLVI--------PR----------- 227 
XP_004989263  285 YLGD-------------------------------------LAPSIFPHD-LTTRVLLT--------PNfyqfe------ 312 
KJE94895      357 TGVDcsgcaarkafcpfcsleyhqlvqdtsvpfrafqpvsdINQFLPSHG-VQMTLLAQgvpvttvvPRtlgstmwtspe 435 
XP_004341525  272 LQGD-------------------------------------FAPWSDNPV-LSEKYLFV--------PSsp--------- 296 

7S0K_A        279 --------pwegtpSYSEKLFFVPSVCTDTSEAWCVd----RI-SYIPTEIN------------------RWMLIDND-- 325  Cyanidioschyz...
XP_020431419  271 --------------NPIHKLPFEMRAMIFSKSDVGS-----PN-ECNKIATD------------------YVAFQNQP-- 310 
CEM12681      385 ------------stGDMLRIGDERGPLILDQSHFDLt----GA-GCDKIGIT------------------YEGFKLQP-- 427 
A0BZ59        240 -------------nSHARVQAGSAAFMIVSKDQV-------GRgECNKIGVS------------------YSAFRTEA-- 279  Paramecium te...
ODN05384      321 -------------yDEPLKGPIEDRILIVPKSLMAPn----GL-SCNRVGTR------------------LQAFISQQ-- 362 
ERL89993      430 ----------pqenQHPEASGGPSEYLVVRETDIDIs----GK-SCNKAGTTwlqlltglndieefegvgFEAFARQP-- 492 
EFJ27200      246 --------------------ADGRLWMLVDEARVTLt----GS-ACDKIGLS------------------CLGYAQQP-- 280 
EXC34107      259 -------------------------------QRLRFtl--dGT-ECNKIGVG------------------YEAFNDQP-- 284 
1_pfamImport  228 -------------------------------QRVRFtl--dGI-ECNKIGVS------------------YEAFNQQP-- 253 
XP_004989263  313 ----------nstdPMFPFRRSPAHWLLLDKTDVDLs----GE-TCNKPGVS------------------FTAFYQHGks 359 
KJE94895      436 irpstffdvrglyqASAFSNISERVMLQSSSEQISTeaqfiGS-SCNQIGVS------------------FQSFTAQS-- 494 
XP_004341525  297 -------------sTHPRVVAGTDYWMLLDRSSADFs----GL-TCNKIGVS------------------FSAFRYQG-- 338 

7S0K_A        326 LVTITGDTCDKIGVSYSAFTNEGQRCErptqsc----------lhdqlqDYYDSDLALEQTGKVGSYFVQFFGDFDVSGL 395  Cyanidioschyz...
XP_020431419  311 NQCSAPLNSCLDNQIKKFRDQDMALYAkgkkgqy--------liknygaTAEIYKNTGNNNLFLQVELSGKQTSLVTIKI 382 
CEM12681      428 DRCSAKKNTCTGRNPNEYRKDDLDKPAlernwllryr---veaesilraVSRTNFTADRSARSVTFKWLGDHSSLITLEA 504 
A0BZ59        280 ERCKKQVKSCLKNQLEDFYIEDQALIAnnsqpk-----------yllsryGKFKSIYLNNETYLQYSVEGSMQTMITLEI 348  Paramecium te...
ODN05384      363 EPCRQERNSCLNNQPFDLWKQDQGPKGapkskfrlksfgn------vnpqlpmnfsdnfLTGHLFMEHKADAPTYVDIEI 436 
ERL89993      493 RRCDNDRGSCLQNQPRQMWQHDHDLETsgrkgcyflknygllpaepvrsKPCNGSSKKTQNKLLYMYYLPPVTSNVNINF 572 
EFJ27200      281 RTCDGALGMCIGEQLIDFIKEDLAALGkgrlai--------------hglfryGSYRSLVPDALQIAVSPT-NSLITIEI 345 
EXC34107      285 NFCSSPFLSCLHNQLWNFWEADQNRIRrkqlpl----------ygvqgrFERINQHPNAGTRSFSIGVTEVLNTNLLIEL 354 
1_pfamImport  254 NFCASPFWSCLHNQLWNYRD-------------------------------------NGGTHSFSIGITEVLNTNLLIEL 296 
XP_004989263  360 GGCNMDPFSCLDNQPAHLVAQDLDRLAagraaqym-------agrlgppLVKRQAVGERVQNMLAFKADIPQESLYTLEI 432 
KJE94895      495 NFCNLPFGSCLQNQISQLRRSAENYTPfhhpala--------gsvscatGLCSRALLQPDNSSLELQVYQVVPLSVTLRL 566 
XP_004341525  339 GACGNWLQACLGNQLDHYHREDLARWEqgqlgryf-------vrfwgdfVGNQAVVQTNDQRYLSFALDQIRATVTTLTL 411 

7S0K_A        396 TPRNP----LLRFFTNRTQATEVVLQf--aAEEL-----FYTIY----LAPARFLRhlSKINPFTSQSKGG--------- 451  Cyanidioschyz...
XP_020431419  383 KADNF----AFVYKESPGVIVSNRLE----TFES-----MSSDG----NLYIQTKN--IGATNTQYVLNVLn-------- 435 
CEM12681      505 KADSL----RWVLQLSPGRIVGLRMA----DFEAlk----------ggDLVVIVQN--TGKLTAEYEIGVSccwgnagcq 564 
A0BZ59        349 TTTGLi---SYVVNLGKGKIDLAEIQ----DFEA-----KSGNG----LLYAQITN--VGDIESEFNTYLN--------- 401  Paramecium te...
ODN05384      437 PADDLiih--------------------spGYNAfienvLTDTGsdgaNIVTQVMN--KELVEAFFSVNLVh-------- 486 
ERL89993      573 AADMN----AILKPDALAMITEVYTEtlnp---------------krvTVTVKILN--SGLVTSVFFVGIGa-------- 623 
EFJ27200      346 AADNV----SFRRNKSTGKIVKVEVP----PFEA-----MSTGG----TLTLTVVN--DGSLEASYGVYVE--------- 397 
EXC34107      355 SADDV----EYVYQRSPGKILSINIP----TFEA-----LSQFG----VATIKTMN--TGEVEASYGLTFD--------- 406 
1_pfamImport  297 SADDI----EFVYQRSPGKILNVTIP----TFEA-----LTQFG----VGTITTMN--TGKVEASYS------------- 344 
XP_004989263  433 EADDSr---IFVLDVSFS------------IFELgvyglASVSR----DIFLWIELarLDKRPSTVYLSVE--------- 484 
KJE94895      567 SANDVvlkePFLSQSRSGWLAAAAAPgcdlDFEPsvvqyQENEG----TLDVSFTN--LGETDGAVGITVT--------- 631 
XP_004341525  412 NADDI----IYTINRSPGRIVVANIT----GFEG-----LATQG----ELDVVVMN--NGTIQADYTITVTe-------- 464 

7S0K_A        452 -------------------L----IDLWivSEGTG---QNAAQFTVSASCEPN-VEPIQAQIVTLAPGQ---------LV 495  Cyanidioschyz...
XP_020431419  436 -------------------C----TEAI--TVNNPsqvFTMKPNEIVETKFEI-RTVTKFGGNQHCYAD---------LK 480 
CEM12681      565 qikqassesndmsslsqeaCsfvsSSYLlhSGAKQ---VTLSPQATREVTFRL-NTETELGGAARCTVG---------ML 631 
A0BZ59        402 -------------------C----SINVipINSAA---LYLKPLESYIVKKDV-NVLSDMNKSNICTFS---------LL 445  Paramecium te...
ODN05384      487 -------------------Ct---VNITqnLKTPQ---SKIGPLNSKTYHMNL--HPYKITDVTHCVVV---------LI 530 
ERL89993      624 -------------------CpleiPASFgsISSQP---VLIAPQHQHIFTLEI-----------YCELPltnfycsleVF 670 
EFJ27200      398 -------------------C----SANInpLEGKR---VSMIPNVPQTFLYTL-ITRSTDATKNSCIVT---------LR 441 
EXC34107      407 -------------------C----SKDVtlMEEQF---FIMKPKEEIIRSFKL-YPATDQAANYD--------------- 444 
1_pfamImport  345 ------------------------------LTEQF---FIMKPNEIAIRSFKL-YPTTDQAAKYICAAI---------LK 381 
XP_004989263  485 -------------------C----SDLVlpVPTTAiswKQTDTGHLRDAIIPL-RTSSTQEVVHTCRVE---------AK 531 
KJE94895      632 -------------------Cq--gLMVQgsPVMMA---ASVNNLTSVRFSVSVpSALNQSYSNASCTATiavq--rlpLW 685 
XP_004341525  465 -------------------C----GDRIqaVQAKM---RSISAYQSANLTFAL-------------YMS---------LY 496 

7S0K_A        496 S-ISLPIIEt---------------------------------------------------KATGGAGVC 513  Cyanidioschyzon merolae
XP_020431419  481 GfAMGTLFDs---------------------------------------------------ILIKFNTTD 499 
CEM12681      632 N-QLAATEDr---------------------------------------------------RTICFNITA 649 
A0BZ59        446 N-NKGTLLDq---------------------------------------------------KQIEFNTTE 463  Paramecium tetraurelia
ODN05384      531 NeHGENIAA----------------------------------------------------REVILHPHQ 548 
ERL89993      671 NmKQQLVAV----------------------------------------------------RRIRFQKSD 688 
EFJ27200      442 D-SEGENCDv---------------------------------------------------KEAKFSTTA 459 
EXC34107      445 --SDYNEVDr---------------------------------------------------AECQFATTS 461 
1_pfamImport  382 D-SNFNEVDr---------------------------------------------------AECQFSTIA 399 
XP_004989263  532 N-ARGRITDt---------------------------------------------------GFVAFKTTA 549 
KJE94895      686 S-ALGKSVScvssipiyagyflppcnltrpgailglsvqnvsldgvdtsvlntngnrtqarLMITFENTG 754 
XP_004341525  497 D-SLGVIVD-----------------------------------------------------SVWVNVTV 512
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