Conserved Protein Domain Family
HAP2-GCS1

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pfam10699: HAP2-GCS1 
Male gamete fusion factor
The gene encoding Arabidopsis HAP2 is allelic with GCS1 (Generative cell-specific protein 1). HAP2 is expressed only in the haploid sperm and is required for efficient guidance of the pollen tube to the ovules. In Arabidopsis the protein is a predicted membrane protein with an N-terminal secretion signal, a single transmembrane domain and a C-terminal histidine-rich domain. HAP2-GCS1 is found from plants to lower eukaryotes and is necessary for the fusion of the gametes in fertilisation. Studies in the green alga Chlamydomonas and the malaria organism Plasmodium showed that it is involved in a novel mechanism for gamete fusion where a first species-specific protein binds male and female gamete membranes together after which a second, broadly conserved protein, either directly or indirectly, causes fusion of the two membranes together. The broadly conserved protein is represented by this HAP2-GCS1 domain, conserved from plants to lower eukaryotes. In Plasmodium berghei the protein is expressed only in male gametocytes and gametes, having a male-specific function during the interaction with female gametes, and being indispensable for parasite fertilisation. The gene in plants and eukaryotes might well have originated from acquisition of plastids from red algae.
Statistics
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PSSM-Id: 402369
View PSSM: pfam10699
Aligned: 14 rows
Threshold Bit Score: 215.007
Threshold Setting Gi: 765554274
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
KJE94895       27 SVAPQSGVVLDTSptnnnaftitFSHNVSMMSGGVLTPLSSLSSSNLNQIFIVAgskpislarnsfvsfpsavsLNLAAP 106  Capsaspora ow...
EFA79298       38 YLKCQKMFVVSLT----------IDSNQDHTELSQFTVNDVKNENGDTFSLVYP--------------------VEISFS 87   Heterostelium...
CEM12681       39 DLDCTEKLVLLLN----------INTGEGGSEYLELGGVSVEDTLGDGERVNLSfs----------------kdIRVKIF 92   Vitrella bras...
ESS30604       53 RMQCKDTILGTLR----------ISNKEKFSFNVMQNTIDSRDKTYADVGNVG---------------------FVVTIT 101  Toxoplasma go...
ODN05384       75 FSTCRKKLLITLR----------ITESTRNFVPTALILIDRAKNTLTQKPSSFGr------------------pIAVKFH 126  Orchesella ci...
EGD79178       53 GGTCKKKLVMTLS----------IGNGQQLPEVVEVTNYIENGQEKPLGQR-----------------------YRIYPT 99   Salpingoeca r...
BAM80469       51 SIPCSKKWVLTLA----------VENGATAASSSVSATQAVYGSSSANATVRSAdnpntv--------yafkyqVHITLT 112  Cyanidioschyz...
XP_004341525   53 TISCDRRMIVTLT----------VDSGQNNTEQLELVLDSTQDEDGVLRTLEHP--------------------VQIQWA 102  Acanthamoeba ...
GAQ85247      233 DITCKNKILVTFI----------VQNGQTQATDSLEATLSAVTDATGTVRQLAEa-------------------YKISIV 283  Klebsormidium...
XP_002971451   41 AIQCKKKILVTVA----------IPSGDGGNGEALIAEVKDPTSRDGKQVLEKS--------------------ISVNIA 90   Selaginella m...
KJE94895      107 GRSLIVSFRLSisasdyYADLMLADVF--------------------------------------------YLTKSGKsl 142  Capsaspora ow...
EFA79298       88 KSKQYGKSSLI------YRKSYSEEKYenvhytndyl-------------------------lfssckdspSDHTCPT-- 134  Heterostelium...
CEM12681       93 KEDVLVSYPLV------YIQDVYDDAAevqnfdnrravggifsstdncetdemklyrkhtedntwvkdnkwNRLSCGVlf 166  Vitrella bras...
ESS30604      102 KTPVTISLPLE------YIKEVPFDYReeiyeys-------------------------------rweagrLPEKFCYed 144  Toxoplasma go...
ODN05384      127 QEKLFQKYHLD------YLYHVNGKMWeevinsenskn-----------------------yaacnnslqtSKPTCGF-- 175  Orchesella ci...
EGD79178      100 KSDAFVNYPLV------YQSSINSLPYehwfkvkrsntqi------------------afffdpcrdaptaEHPTCGY-- 153  Salpingoeca r...
BAM80469      113 KSRIRLDYPLY------YQSDFNNKPYeivykynqkgpln-------------------wldnqcvatwgsSDPTCGYay 167  Cyanidioschyz...
XP_004341525  103 KTIPRLLYPIT------YVGRTNNKPYetitykddilf----------------------lfdecndspssSSPTCGW-- 152  Acanthamoeba ...
GAQ85247      284 KTPVYATYPLS------FLRSFNAKPYeeiiltnq-----------------------------cqdgalaDQATCGW-- 326  Klebsormidium...
XP_002971451   91 KTDSIVKYALE------YLKNVAGDLNervikkkk-----------------------------gcntklnDKATCGV-- 133  Selaginella m...
KJE94895      143 pfsspatyrlqNVAPDTCLANIASPVTSLCSfvdassvpstp----------------------------------vssp 188  Capsaspora ow...
EFA79298      135 -----------VRDASGNQVPYSQGFCCSCDlgsyvgidrdsrshlsctlfgg------------rsssascmaqrplly 191  Heterostelium...
CEM12681      167 kde----gepdGPDRKYEPIPSSGGFCCFCDnfdaafptiewkrglqlecwtf-----------nspwschamrygeeks 231  Vitrella bras...
ESS30604      145 ttdkcsedgklAVHPHGKPLSWAHGRCCWCSevlafthinnm---------------------------------krgnf 191  Toxoplasma go...
ODN05384      176 ----------------IHGTSEVTGFCCSCKdhkgnvqqrsghrcdvpgktpn------------lltsahclrsspfty 227  Orchesella ci...
EGD79178      154 ------------FVRKGERIPNSQGFCCKCKffsgggnnayrgnlkcrlysp-------------hyssahclrfwpnwy 208  Salpingoeca r...
BAM80469      168 n--------psWSTKPADRILYSQGFCCDCNagdllglspnrirggldcsllnf---------dnptesahclrfdslwy 230  Cyanidioschyz...
XP_004341525  153 -----------FYNADGTVVRDSQGFCCSCDlsevlwlsneqtragltcslfaf---------gvdgssahclrfdqlwy 212  Acanthamoeba ...
GAQ85247      327 -----------YYAQDGSRVPNSQGFCCKCGvdqtwsdttggtstqqrrfavncnvltnslfltgipatasclrmsadwy 395  Klebsormidium...
XP_002971451  134 -----------LGDSKGNVVPGSSGFCCTCKplkqikhfrgmpkpghcgisd--------------agyafclrfgqmwc 188  Selaginella m...
KJE94895      189 twsgtiNEELAVNISVNIQNSGSassslgaiklthqdveyellsdwvvsiqrndtkfvvptipntqryydamevsrgria 268  Capsaspora ow...
EFA79298      192 dsysiePPVTTYDIVVNITQFNV--------------------------------------------------------- 214  Heterostelium...
CEM12681      232 eiyrvqTHKWQWSISITMYDIAAmymceeilggvsgyeakshlccpkacgecgndecdkapggatnccaapmlssnrncs 311  Vitrella bras...
ESS30604      192 rcnwfaPPRALELVTETLYDQCEagkidgtvpl----------------------------------------------- 224  Toxoplasma go...
ODN05384      228 nvykmsPPTIYQKVSFEVFKSKQnln------------------------------------------------------ 253  Orchesella ci...
EGD79178      209 daweldRAQVSHTIRLAVYKETSfngtipeptqac--------------------------------------------- 243  Salpingoeca r...
BAM80469      231 safqigEPDVNFVILVNVTKCPLanstiksis------------------------------------------------ 262  Cyanidioschyz...
XP_004341525  213 dvfsigAAQVSFEVVLSVKKYQTmtdm----------------------------------------------------- 239  Acanthamoeba ...
GAQ85247      396 payligAASLDFQLQITVNRMLPsdn------------------------------------------------------ 421  Klebsormidium...
XP_002971451  189 vmfrirTGTISFEITITLTDQNGn-------------------------------------------------------- 212  Selaginella m...
KJE94895      269 nstylaglvdtsvssatvqtFLNAASAGSLTLivlnlapeqawdyvdswtttsqyesglstaqlrfanlsaGTSTFINFN 348  Capsaspora ow...
EFA79298      215 --------------------TTGASQTQTYRL---------------------------------------GNDNLILNA 235  Heterostelium...
CEM12681      312 shappcvvsfgdsgpshsssSFREPTAWTATI---------------------------------------NPNRKVAEA 352  Vitrella bras...
ESS30604      225 -----------drdcerekhERLGITDRVYTL---------------------------------------NYTTPEIFD 254  Toxoplasma go...
ODN05384      254 -----------------gtiSWEQLSHTKFGV---------------------------------------TVDSDNLVN 277  Orchesella ci...
EGD79178      244 --------kdsnarptrrvnGFAFYCVDVLLI---------------------------------------GVQNKVARS 276  Salpingoeca r...
BAM80469      263 ------------glvgnqdqAIQNCSTEIISL---------------------------------------SPSSPIGYA 291  Cyanidioschyz...
XP_004341525  240 -----------------ygnTTGGYVTETLRL---------------------------------------SPSQTTGTA 263  Acanthamoeba ...
GAQ85247      422 ------------------grVSNGSAVQTLIL---------------------------------------SPSVPVAKT 444  Klebsormidium...
XP_002971451  213 -------------------kASSRIIGFVLRL------------------------------------------TLLAEK 231  Selaginella m...
KJE94895      349 SvwTIRQETGVDcsgcaarkafcpfcsleyhqlvqdtsvpfrafqpvsdINQFLPSHG-VQMTLLAQgvpvttvvPRtlg 427  Capsaspora ow...
EFA79298      236 N--GIVIKLVGD-------------------------------------FASPQALRT-FEDSMLFVnne--qsdPN--- 270  Heterostelium...
CEM12681      353 Q--SIIVKYQGS-------------------------------------LLPDDGVPTwLEDKYVVW--------PAed- 384  Vitrella bras...
ESS30604      255 r------------------------------------------------------------------------------- 255  Toxoplasma go...
ODN05384      278 VsgDVKVSYRAHse----------------------------------gIAASATIFD-ASDQRLLIplpnpfvkPEn-- 320  Orchesella ci...
EGD79178      277 TngTVIATYLGD-------------------------------------LAPSIFPHD-LTTRVLLT--------PNfyq 310  Salpingoeca r...
BAM80469      292 SngKISAQAIGD-------------------------------------FAPWEGTPS-YSEKLFFV--------PSvct 325  Cyanidioschyz...
XP_004341525  264 AggDIFAKLQGD-------------------------------------FAPWSDNPV-LSEKYLFV--------PSsp- 296  Acanthamoeba ...
GAQ85247      445 TegNVLARLPGD-------------------------------------LASYTQLPV-FSDRYLMI--------PQta- 477  Klebsormidium...
XP_002971451  232 PdgNWQQFTRGS---------------------------------------------------------------PR--- 245  Selaginella m...
KJE94895      428 stmwtspeirpstffdvrglyqASAFSNISERVMLQSSSEQISTeaqfiGSSCNQIGVSFQSFTAQS--NFCNLPFGSCL 505  Capsaspora ow...
EFA79298      271 ----------------------NPIHKLPFEMRAMIFSKSDVGS-----PNECNKIATDYVAFQNQP--NQCSAPLNSCL 321  Heterostelium...
CEM12681      385 --------------------stGDMLRIGDERGPLILDQSHFDLt----GAGCDKIGITYEGFKLQP--DRCSAKKNTCT 438  Vitrella bras...
ESS30604      256 ---------------------sVYCNTKSCLKHAIILDKDYVSVt----GYECDKVGTGLDRWGdm-rgEFCNLLPGTCI 309  Toxoplasma go...
ODN05384      321 ---------------------yDEPLKGPIEDRILIVPKSLMAPn----GLSCNRVGTRLQAFISQQ--EPCRQERNSCL 373  Orchesella ci...
EGD79178      311 fe----------------nstdPMFPFRRSPAHWLLLDKTDVDLs----GETCNKPGVSFTAFYQHGksGGCNMDPFSCL 370  Salpingoeca r...
BAM80469      326 dts-------------eawcvdRISYIPTEINRWMLIDNDLVTIt----GDTCDKIGVSYSAFTNEG--QRCERPTQSCL 386  Cyanidioschyz...
XP_004341525  297 ---------------------sTHPRVVAGTDYWMLLDRSSADFs----GLTCNKIGVSFSAFRYQG--GACGNWLQACL 349  Acanthamoeba ...
GAQ85247      478 ---------------------aQAGRLTANTSTWMFIDKTQVNLd----GTVCNMIGTSFTAFRNQP--NRCEQYAGTCL 530  Klebsormidium...
XP_002971451  246 ----------------------------ADGRLWMLVDEARVTLt----GSACDKIGLSCLGYAQQP--RTCDGALGMCI 291  Selaginella m...
KJE94895      506 QNQISQLRRSAENYTPfhhpal---------agsvscATGLCSRALLQPDNSSLELQVYQVVPLSVTLRLSANDVvlkeP 576  Capsaspora ow...
EFA79298      322 DNQIKKFRDQDMALYAkgkkgq---------yliknyGATAEIYKNTGNNNLFLQVELSGKQTSLVTIKIKADNF----A 388  Heterostelium...
CEM12681      439 GRNPNEYRKDDLDKPAlernwllry----rveaesilRAVSRTNFTADRSARSVTFKWLGDHSSLITLEAKADSL----R 510  Vitrella bras...
ESS30604      310 TGQLRKFKEVDKLRIEqnlaplyalkrefggfpryapnpmNGTGFSTTGTRHYLGYDFGEQHYSDIRFEMDATDV----T 385  Toxoplasma go...
ODN05384      374 NNQPFDLWKQDQGPKGapkskfrlksfgn-------vnpqlpmnfsdnfLTGHLFMEHKADAPTYVDIEIPADDLiih-- 444  Orchesella ci...
EGD79178      371 DNQPAHLVAQDLDRLAagraaqy--------magrlgPPLVKRQAVGERVQNMLAFKADIPQESLYTLEIEADDSr---I 439  Salpingoeca r...
BAM80469      387 HDQLQDYYDSDLALEQtgkvgs-----------yfvqffGDFDVSGLTPRNPLLRFFTNRTQATEVVLQFAAEEL----F 451  Cyanidioschyz...
XP_004341525  350 GNQLDHYHREDLARWEqgqlgry--------fvrfwgDFVGNQAVVQTNDQRYLSFALDQIRATVTTLTLNADDI----I 417  Acanthamoeba ...
GAQ85247      531 ANQLVDLAKADAARVAaglvpq------------ylvsgyGISTGAADSSGLKLKFVVNQILSSVVSLELNADEA----R 594  Klebsormidium...
XP_002971451  292 GEQLIDFIKEDLAALGkgrlai---------------hglfryGSYRSLVPDALQIAVSPT-NSLITIEIAADNV----S 351  Selaginella m...
KJE94895      577 FLSQSRSGWLAAAAAPgcdlDFEPsvvqyQENEG-------TLDVSFTN--LGETDGAVGITVT---------------- 631  Capsaspora ow...
EFA79298      389 FVYKESPGVIVSNRLE----TFES-----MSSDG-------NLYIQTKN--IGATNTQYVLNVLn--------------- 435  Heterostelium...
CEM12681      511 WVLQLSPGRIVGLRMA----DFEAlk-------------ggDLVVIVQN--TGKLTAEYEIGVSccwgnagcqqikqass 571  Vitrella bras...
ESS30604      386 WLRATSPGHITFIEVPq------------LDACSsstiggcPLKAYVWN--SGNEDAAFAVEVPf--------------- 436  Toxoplasma go...
ODN05384      445 ------------------spGYNAfienvLTDTGsd---gaNIVTQVMN--KELVEAFFSVNLVh--------------- 486  Orchesella ci...
EGD79178      440 FVLDVSFS------------IFELgvyglASVSR-------DIFLWIELarLDKRPSTVYLSVE---------------- 484  Salpingoeca r...
BAM80469      452 YTIYLAPARFLRHLSKi--nPFTS-----QSKGG-------LIDLWIVSegTGQNAAQFTVSAS---------------- 501  Cyanidioschyz...
XP_004341525  418 YTINRSPGRIVVANIT----GFEG-----LATQG-------ELDVVVMN--NGTIQADYTITVTe--------------- 464  Acanthamoeba ...
GAQ85247      595 YLVNRSPGKIVSAVVP----TFEA-----VSRNG-------NMAITVQN--NGTLVADYTLTVQn--------------- 641  Klebsormidium...
XP_002971451  352 FRRNKSTGKIVKVEVP----PFEA-----MSTGG-------TLTLTVVN--DGSLEASYGVYVE---------------- 397  Selaginella m...
KJE94895      632 ------------Cq------gLMVQgsPVMMA---ASVNNLTSVRFSVSVpSALNQSYSNASCTATiavq--rlpLWS-A 687  Capsaspora ow...
EFA79298      436 ------------C--------TEAI--TVNNPsqvFTMKPNEIVETKFEI-RTVTKFGGNQHCYAD---------LKGfA 483  Heterostelium...
CEM12681      572 esndmsslsqeaCsf----vsSSYLlhSGAKQ---VTLSPQATREVTFRL-NTETELGGAARCTVG---------MLN-Q 633  Vitrella bras...
ESS30604      437 ------------CidsitkerTIDVnpITPVR---TTVPADKTVVFTLTF-KAISSSSLGVTCFMK---------LYD-A 490  Toxoplasma go...
ODN05384      487 ------------Ct-------VNITqnLKTPQ---SKIGPLNSKTYHMNL--HPYKITDVTHCVVV---------LINeH 533  Orchesella ci...
EGD79178      485 ------------C--------SDLVlpVPTTAiswKQTDTGHLRDAIIPL-RTSSTQEVVHTCRVE---------AKN-A 533  Salpingoeca r...
BAM80469      502 ------------C--------EPNVepIQAQI---VTLAPGQLVSISLPI-IETKATGGAGVCNCT---------LRN-A 547  Cyanidioschyz...
XP_004341525  465 ------------C--------GDRIqaVQAKM---RSISAYQSANLTFAL-------------YMS---------LYD-S 498  Acanthamoeba ...
GAQ85247      642 ------------C--------SDNIlpIQAQM---HSIAPNATQDFAFPV-VVQDDQATQRNCTGKrsgivfkiiLYD-S 696  Klebsormidium...
XP_002971451  398 ------------C--------SANInpLEGKR---VSMIPNVPQTFLYTL-ITRSTDATKNSCIVT---------LRD-S 443  Selaginella m...
KJE94895      688 LGKSVScvssipiyagyflppcnltrpgailglsvqnvsldgvdtsvlntngnrtqarLMITFENTGipalavvktlctp 767  Capsaspora ow...
EFA79298      484 MGTLFDs---------------------------------------------------ILIKFNTTDtvikvpgynetgs 512  Heterostelium...
CEM12681      634 LAATEDr---------------------------------------------------RTICFNITAtestlgqqeegei 662  Vitrella bras...
ESS30604      491 QHLMLDq---------------------------------------------------KTFNVTTSAaqedkdglsnfer 519  Toxoplasma go...
ODN05384      534 GENIAA----------------------------------------------------REVILHPHQ------------- 548  Orchesella ci...
EGD79178      534 RGRITDt---------------------------------------------------GFVAFKTTA------------- 549  Salpingoeca r...
BAM80469      548 LGQVLDv---------------------------------------------------LVLEFNASSvrttdgaqggsas 576  Cyanidioschyz...
XP_004341525  499 LGVIVD-----------------------------------------------------SVWVNVTVfatnitclggqcs 525  Acanthamoeba ...
GAQ85247      697 VGEITDi---------------------------------------------------STVFFYTNAtfydkgaqggnlt 725  Klebsormidium...
XP_002971451  444 EGENCDv---------------------------------------------------KEAKFSTTAtvfnngsqvggvq 472  Selaginella m...
KJE94895      768 gyvlvgvpgniylsgasv---------------gvsafvlrstapgvsstcschfevyahhptpNCSVIVSQA 825  Capsaspora owczarzak...
EFA79298      513 g-------------------------------------------------dtpnpavpelscqnYCTLMYLEH 536  Heterostelium album ...
CEM12681      663 gglgagdykpd-----------------------------fqyekdkiyvevtcrdicpsfwalACFVDNLDI 706  Vitrella brassicafor...
ESS30604      520 ffgi--------------------------------------------trpsgpavcpcrwyngVCLLFRFRT 548  Toxoplasma gondii VEG
ODN05384      549 ----------------------------------------------------------------TCICVHYCK 557  Orchesella cincta
EGD79178      550 ----------------------------------------------------------------TCACLGLCD 558  Salpingoeca rosetta
BAM80469      577 ttsgnlth------------------------------------tgsspypsggcgscgglldiGCIFANVCI 613  Cyanidioschyzon mero...
XP_004341525  526 dgsgggkp-----------------------------------adggykyaitscsacnsifdiACYVDNSCM 563  Acanthamoeba castell...
GAQ85247      726 vgqttnstvv--------------------------------qaqspptaaltcvqlcpsifsvSCAFLTGCW 766  Klebsormidium nitens
XP_002971451  473 iagskndtfakglgglgffgkigagikgiakgafnvvtspfrkmfglfnnllgkcdncpgafdiGCFIAHFCV 545  Selaginella moellend...
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