4CKB


Conserved Protein Domain Family
MCEL_TPase
?
pfam10640: MCEL_TPase 
mRNA capping enzyme catalytic subunit, RNA triphosphatase domain
mRNA-capping enzyme catalytic subunit from Vaccinia virus (MCEL, also known as mRNA-capping enzyme D1 subunit) catalyzes the hydrolyzis of the 5' triphosphate end of the pre-mRNA, the capping of the resulting diphosphate RNA end with GMP and the methylation of the GpppN cap. It consists of an N-terminal RNA triphosphatase (TPase)-guanylyltransferase (GTase) module, and a C-terminal guanine-N7-methyltransferase (MTase) module (pfam03291). The N-terminal module comprises a TPase domain, represented in this entry, followed by the GTase at the C-terminal, which consists of the nucleotidyltransferase (NTPase, pfam21004) and OB fold (pfam21005) domains. This domain catalyzes the hydrolyzis of the the 5' triphosphate end of the pre-mRNA.
Links
?
Statistics
?
PSSM-Id: 491315
Aligned: 11 rows
Threshold Bit Score: 208.772
Created: 15-Feb-2025
Updated: 28-Apr-2025
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
4CKB_A  27 QRSTAYEINNELELVFIKPPLITLTNVV--NISTIQESFIRFTVTNk--EGVKIRTKIPLSKVHGLDVKNVQLVDAIDNI 102  Vaccinia virus
Q9Q8M4  24 NPVQTDDINHEVELTFIHPPIIALSTLI--HVATSQESYILFTVTN---KGVKIRNRINLSKIHGLDLKNIQLVDSIDNI 98   Myxoma virus
P04298  27 QRSTAYEINNELELVFIKPPLITLTNVV--NISTIQESFIRFTVTNk--EGVKIRTKIPLSKVHGLDVKNVQLVDAIDNI 102  Vaccinia virus WR
Q6TVQ6  22 RGAPEDEVHHEVELVLVDPPLSTLAATL--RLASETESFILFTVTAlakEEGKLRARVPMSRVVGLDVKNVQLVNAIDSI 99   Orf virus
Q98257 128 aADTTSDVHHEVELIFVRPPLVTLSHLL--GIATRQESYVLFSLTSlerEGVKLRTRLPLARVHGLDMKNNQLVEAIDSI 205  Molluscum contagiosu...
Q9EMR4  20 NFDSNPNVNNEIEFTYINPDLRILSNIYtdNNESKKKTYLEYVLKFa-nKKSKLRQKYKY-DYPTFEIANSYFLDKLTN- 96   Amsacta moorei entom...
Q070E2  26 ENRFDAARHNEVELILLNPPLSAVSVLH--TIATVEESYVQYVLKA---KDAKIRNRLSVKDVHGLDVKNYQLVERLDDA 100  Crocodilepox virus
Q6TUT6  26 yPLPTEDVNNEVELVFIRPPLITLGNVL--NISSVQESFIMFTVGNk-eKKEKIRTNVPLSKVHGLDIKNVQLVESVNNI 102  Yaba monkey tumor virus
Q8V3L9  26 QPVHLDDVNHEVEMIFTNPSIVTLSNLI--NISSTQESYILFTLTRk-dEHTKIRTKIPMSKIHGIDIKNIQLVNCIDNI 102  Swinepox virus
Q08FR4  26 KPKSLDDINHEVEIVFIQPPLITLNNVL--NISSKQESYILFSLTNk-eDRVKIRSYLPMSKVYGLDLKNIQLVDSIDNI 102  Deerpox virus W-848-83
Q9J584  28 INKVDESKHHEVELILFKPPIITLTNLY--NMATTTESYIEFTMLPvdkPNTKFRNRIPLSKIHGLDVKNNQLVESLDGF 105  Fowlpox virus strain...

4CKB_A 103 VWEKKSLVTENR--L-H-KECLLRLSTEERHIFLDYKKYGSSIRLELVNLIQAKTKN-------------FTIDFKLKYF 165  Vaccinia virus
Q9Q8M4  99 IWEKKTLVKEHK--V-D-PAAVVKYSTEEKYIFLDYKKYLSSIKLELVNVVQAKVKH-------------VTVDFKFKYF 161  Myxoma virus
P04298 103 VWEKKSLVTENR--L-H-KECLLRLSTEERHIFLDYKKYGSSIRLELVNLIQAKTKN-------------FTIDFKLKYF 165  Vaccinia virus WR
Q6TVQ6 100 VWERKALVEETA--L-Q-EGCLLRHSTERRHLFVDYKKYLSAIRVELVNRVRVRSKE-------------VVVDFKFKYF 162  Orf virus
Q98257 206 LWEKKTRVAQRD--FpE-AHCLLRHSTEEQYVFLDFRNFNSAIRLELVNHVQARVRN-------------IVVDFKLKYF 269  Molluscum contagiosu...
Q9EMR4  97 NWERKTIISEDKinInKnEYILLRHNTEYQ---------DNDIELPLLNDILDKINVlfvsqlyiiindlIKVEFKIKSN 167  Amsacta moorei entom...
Q070E2 101 NWERKAPVWEKS--F---PGATIKHSTEESHPFTDYRNYSGNIKLEFVNLVRARAKH-------------LIVDAKFKYF 162  Crocodilepox virus
Q6TUT6 103 IWEKKTLLSEYK--V-N-DNCLIRHSIEEKHVFLDYKKYISSIKLELVNLIQAKIKN-------------VKVDFKLKYF 165  Yaba monkey tumor virus
Q8V3L9 103 IWEKKIRVKENI--I-D-DWCIVQYSTEEKYIFLDYKKFTSSIKLELVNLIQARIKN-------------IVIDFKIKYF 165  Swinepox virus
Q08FR4 103 IWEKKTLITEHI--V-D-KHCMVRYSTEEKHIFLDYRKYISAIRLELVNLIQLKIKN-------------ILIDFKIKYF 165  Deerpox virus W-848-83
Q9J584 106 IWEEKSLLLKKD--IsDnSSAIIKYSIEEKTLFVDYKRRNASIKLELVSVVRAKLRN-------------IVIDFKMKYF 170  Fowlpox virus strain...

4CKB_A 166 LGSGAQS--KSSLLHAINHPKS-RPNTSLEIEFTPrDN----ETVPYDELIKELTTLSRHIFMA-S 223  Vaccinia virus
Q9Q8M4 162 LGSGAQA--KSSLLHVLNHPKS-KPNPSLEFEIVT-TD----EKIDSVSLRKELVALFKLVFMT-S 218  Myxoma virus
P04298 166 LGSGAQS--KSSLLHAINHPKS-RPNTSLEIEFTPrDN----ETVPYDELIKELTTLSRHIFMA-S 223  Vaccinia virus WR
Q6TVQ6 163 LGSGAQA--KSSLLHALNHPKV-RPSPTLEFEVVP-AG----EAVDEAAVLTELRAVAKALFMA-P 219  Orf virus
Q98257 270 LGSGAQT--RNSLLAVLNYPKF-RPSVTLEFEVLLrDP----AAFSPRELLRELLVLGNLLFMA-P 327  Molluscum contagiosum virus subtype 1
Q9EMR4 168 IGPLSSN--KLLLSTHFNDIETyRKNITYYLEIEVlSK----TKLDNNVLYDNLVKSFEYIYKSkN 227  Amsacta moorei entomopoxvirus
Q070E2 163 IGNGSQN--YNSLLYALNDPAA-KPGVTLEFEIDVsREhlerEGFSFAELRASLYTVCQALFLA-P 224  Crocodilepox virus
Q6TUT6 166 LGSGAQA--KSSLLHVLNHPRS-KPSPSLEFEIVN-TD----ININENELFNELHALFRSIFMN-L 222  Yaba monkey tumor virus
Q8V3L9 166 LGSGAQS--KSSLLHVLNHPKS-KPKTSLEIEIVT-RD----IKIDTSVLSNEIHTICKILFMS-D 222  Swinepox virus
Q08FR4 166 LGSGAQA--KSSLLHVLNHPKS-KPNVSLEFEIISeND----YKIDKDIIFNEISTLFRNIFMG-N 223  Deerpox virus W-848-83
Q9J584 171 LGSGAQSanSSSLLCALNHPKN-KPSLYIEFEIMM-QD----KNISKKKLLEELNMSASALFLS-H 229  Fowlpox virus strain NVSL
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap