4YXJ


Conserved Protein Domain Family
DisA-linker

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pfam10635: DisA-linker (491311 is an unknown moldel)
DisA bacterial checkpoint controller linker region
The DisA protein is a bacterial checkpoint protein that dimerizes into an octameric complex. The protein consists of three distinct domains. the first, N-terminal region, from 1-145 is globular and is represented by family DisA_N, pfam02457; the next 146-289 residues is this domain that consists of an elongated bundle of three alpha helices (alpha-6, alpha-10, and alpha-11), one side of which carries an additional three helices (alpha7-9), thus forming a spine like-linker between domains 1 and 3. The C-terminal residues of domain 3 are family HHH, pfam00633, the specific DNA-binding domain. The octameric complex thus has structurally linked nucleotide-binding and DNA-binding HhH domains and the nucleotide-binding domains are bound to a cyclic di-adenosine phosphate such that DisA is a specific di-adenylate cyclase. The di-adenylate cyclase activity is strongly suppressed by binding to branched DNA, but not to duplex or single-stranded DNA, suggesting a role for DisA as a monitor of the presence of stalled replication forks or recombination intermediates via DNA structure-modulated c-di-AMP synthesis.
Statistics
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PSSM-Id: 491311
Aligned: 97 rows
Threshold Bit Score: 118.002
Created: 21-Aug-2024
Updated: 4-Dec-2024
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
4YXJ_B       165 NQVDFLISKVTQAISTLEKYKDNFNKLLSELEVLELENRVTLADVVRTLAKGFELLRIVEEIRPYIVELGEEGRLARMQL 244 Thermotoga mari...
WP_014297200 143 ypeTIILPRLNQEIAVAQRYKQSFFELLSEINIAEMENRVILSTVVEAISKGFMTLKVAQKAEKYLLELGEAAESSKLEI 222 Marinitoga
WP_014729964 140 NNVETLTSKITQTIRTAEKYKEAFIEALQGIEFMERTGSLTLYDVVKTIQKGLMVALICEEAEFTIAELGNHGRFPSMQL 219 Mesotoga prima
WP_012002838 140 NDAPLLIARVSQAVDTLEKYRESFDRIIGTIEIDELRTGVSIYDVARAIEKGLLMLRIHEEIFPYVIELGGEGRLINMQL 219 Pseudothermotoga
WP_013931941 140 NDRRLLMTKSTQALNALEKYRTSFEKIFSTIENEELRG-ISIYDVARAIEKALIMLRIWEDIYPYIVELGEDGKLISLQM 218 Pseudothermotog...
WP_011993277 145 ENINFVLTKVGQTINALERFREEFDKNLLTLDKLEVEGNVPLDFVCETLMRGIEIKVISNNISVNIIELGNEGRLSSLRL 224 Fervidobacteriu...
WP_012861904 140 lDMKDILAKTSQALMALEKYATAIHHHLTNLTILEFDNMVTVNEVVEGIKKYGLLYRITDELDEYILELGSEGRLIVLQY 219 Sebaldella term...
Q8RGW9       139 KNFDELNIEVGQVLKTLESYRHVLNRSLDSLTILELDDLVTVLDVANTLQRFEMVRRISEEITRYLLELGSRGRLVNMQV 218 Fusobacterium n...
WP_013252484 150 RSVPDIMSSVNQLLVSMQSTRGQLDRTLLRLTTLELDNYVTLSDIAETIYLFEVLMTASEELDSLILQLGSEGKTIAMQR 229 Olsenella uli
WP_012809269 150 KSVPAIMSTVNQLSVAMQNTRQQLDRALLRLTALELDNYVTLGDVAGIFYLFEVLMSAADQLDACLLELGSEGKTTAMQR 229 Lancefieldella ...
4YXJ_B       245 RELTEDVDDLLVLLIMDYSSEEVeEETAQNILQDF---ITRREPSPISISRVLGYDVQ 299 Thermotoga maritima
WP_014297200 223 NEIYRITPRYLGALIMDYSKDLLdFQYPQEALKLFdgfKTEEFLNMKLISQKLGYDIE 280 Marinitoga
WP_014729964 220 AEILVNVNEEIESLILDFHEELLdQDSLEEVFDKMrslGEKEISSFGVILRNLGYDIN 277 Mesotoga prima
WP_012002838 220 EEVLEDVTDITSLLIMDYSIRDLdVDQAKEIIEKM---KQDKEISLMRIIRTLGVDVQ 274 Pseudothermotoga
WP_013931941 219 SELLEGLDDIVFLLVMDYSFDELkMEDAKRLISKM---KEERDLSTLRIVKYLGWDVQ 273 Pseudothermotoga thermarum
WP_011993277 225 REVLKDLNEILTLMIMDYSKRDLsEEEAKNVLESItk-lEHRLLH---IAKALGYDLN 278 Fervidobacterium nodosum
WP_012861904 220 EELMQGIKEDLYDLIRDYNNS---EETVDKIFSDIrklNKEELLELGKIAHIMGFGKA 274 Sebaldella termitidis
Q8RGW9       219 SELIWDLDEEEESFLKDYIDD---ETDTDSVRRYLhslSDSELLEVENVVVALGYSKS 273 Fusobacterium nucleatum subsp. nuclea...
WP_013252484 230 EEFIGSASYDYTLLIRDYAADSS-EENAAAIRRALhetVNTQLRSSKRVGALLGYEGK 286 Olsenella uli
WP_012809269 230 EEYLGGIDEAYNLMIRDYAVDSS-AEEARAIRRRFhetANTELRSAESVGQILGYSDG 286 Lancefieldella parvula
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