Conserved Protein Domain Family
CTU2
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pfam10288: CTU2 (This model is not part of the current CDD release)
Cytoplasmic tRNA 2-thiolation protein 2
CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside. This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing.
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Statistics
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PSSM-Id: 519300
Aligned: 96 rows
Threshold Bit Score: 58.7082
Created: 15-Feb-2025
Updated: 28-Apr-2025
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
XP_011388644 431 SIARLTETFILNLEKGVPSTVTTIGKTGSKLVLNGAPgfptttwtdvahstsafrdvgpsvslssrlthrmanvtlaetq 510
XP_007477363 362 SIHRLMESFILKLQAQYPSTISTVYRTSEKLVKAPRNgeat--------------------------------------- 402
XP_003460962 348 SIHRLMEAFILQLQAQFPSTVSTVYRTSEKLVKAPREdca---------------------------------------- 387
Q3SZG9       335 SIHRLMEAFILRLQAQFPSTVSTVYRTSEKLVKApra------------------------------------------- 371 domestic cattle
XP_002913441 344 SIHRLMEGFLLRLQAQFPSTVSTVYRTSEKLVKAPRDgc----------------------------------------- 382
7_pfamImport   1 SIHRLMEAFILRLQTQFPSTISTVYRTSEKLVKApregr----------------------------------------- 39 
Q3U308       347 SIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKApregc----------------------------------------- 385 house mouse
EGV94077     253 SIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKAprdsc----------------------------------------- 291
5_pfamImport   1 SIHRLMEAFLLRLQTQFPSTVSTVYRTSEKLAKAprdsh----------------------------------------- 39 
4_pfamImport   1 SIHRLMEAFVLRLQAQFPSTVSTVYRTSEKLVKApragq----------------------------------------- 39 

XP_011388644 511 ceprigsrgiklaqlsancvrwnSINGCALCAMPSqhPKARWWKRDITISSLAetAPRSRQTVETQATCQSSEQADWLEL 590
XP_007477363 403 --------------------agpVPSRCLLCVCAL--DIDSADSATAFGAQTAehLSQKPLLAPELPAGAPGRPCCCSRT 460
XP_003460962 388 --------------------gepEGPRCLLCLCIL--DIDAADSATAFGAQTSt-LSQMPPSALQAEAGTRTAPCCAVGR 444
Q3SZG9       372 --------------------gcaAGPRCLLCMCTL--DVDTADSATAFGAQTSh-LPQMQ--TPVT-----------QAR 415 domestic cattle
XP_002913441 383 ----------------------aAGPRCLLCMCLL--DVDTADSATAFGAQTQdcSQMRPPTPPAEAPTVPCCSTEVGRV 438
7_pfamImport  40 -------------------aagpAGPRCLLCLCAL--DVDTDDSATAFGAQTSr-LSQTLLSTPPA-----------EAG 86 
Q3U308       386 -------------------aagpSGPSCLLCMCAL--DIDTADSATAFGAQSSshLSQM----PSA-----------EAG 429 house mouse
EGV94077     292 -------------------atgpSGPNCLLCMCTL--DIDTADSATAFGAQSSshLSQM----LPA-----------EAG 335
5_pfamImport  40 -------------------dtahSEPRCLLCLCTL--DVDGADSATAFGAQTSshLSQMQPHTALA-----------EAG 87 
4_pfamImport  40 -------------------aaspSRPCCLLCLCTL--DIDSADSATAFGAQTAtaLSLRQ-----A-----------QAK 82 

XP_011388644 591 CNH---------------------------------LCYACLL 600
XP_007477363 461 EGQqgccqvirlpse----------rkeprasfieqLCYSCRV 493
XP_003460962 445 APGccwkacrreh--------------dsqtqviqqLCYSCRG 473
Q3SZG9       416 AAAgpcccagmggapgc------ckredpraqvmeqLCYGCRV 452 domestic cattle
XP_002913441 439 QNCfrgtkpcgre--------------dsrarvieqLCYSCRV 467
7_pfamImport  87 TTTgpgcvagegqaccraa-cnyvhrkgvqaqvmeqLCYGCRV 128
Q3U308       430 MPTqpccaagegqaqschr--evgkrgdaraciteqLCYSCRV 470 house mouse
EGV94077     336 MPTppncgagegqaqschr--gvgrredvqacimeqLCYGCRV 376
5_pfamImport  88 MHPvpccppevgsaqgccr--gpcergdprvcvlewLCYGCRV 128
4_pfamImport  83 EPTvpccspglcgdqgccrgvrpsereelrarvieeLCYSCRV 125
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