Conserved Protein Domain Family
Exo5

?
pfam09810: Exo5 
Exonuclease V - a 5' deoxyribonuclease
Exonuclease V is a monomeric 5' deoxyribonuclease that is localized in the nucleus. It degrades single-stranded, but not double-stranded, DNA from the 5'-end, and the products are dinucleotides, except the 3'-terminal tri- and tetranucleotides, which are not degraded. The initial hydrolytic cut of exonuclease V on the dephosphorylated substrate produces a mixture of dinucleoside monophosphates and trinucleoside diphosphates. The enzyme is processive in action. Exo5 is specific for single-stranded DNA and does not hydrolyze RNA. However, Exo5 has the capacity to slide across 5' double-stranded DNA or 5' RNA sequences and resume cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively.
Statistics
?
PSSM-Id: 401678
View PSSM: pfam09810
Aligned: 66 rows
Threshold Bit Score: 233.701
Threshold Setting Gi: 74636158
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
?
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
GAA87462             175 SPVERF-RRPpMKAFSVTDLISPAWCELQYWYTLTKF----GRKPRTAAMKLGSKIHKTLEDEI---Y----------TT 236  Asperg...
XP_011109951         263 SPLDRWrSKAgLSKLSVSDLLSNMWCEQQHHYTLLR-----GFKRRTAEMQAGTKIHREIEEEI---H----------TV 324  Dactyl...
CCX04512             127 SPYERF-RSK-KKYLSVSDLVGLIWCEQQYEYQLSV-----GWKTVTPEMTRGTAVHKALEEQV---H----------TA 186  Pyrone...
WGS:AATM:SJAG_05280   79 nlyeMF-RSD-RGYLKVTDMVAPLWCGVQHSYALLD-----RVERKTPAMEKGTIIHLKLELETtppK----------ER 141  Schizo...
EPY53777              63 tlydlF-RKR-RGYLNVTDLVSPLWCEVQQDYYLTK-----KIKKTSPQMIKGSSVHQHLEYETtppT----------DR 125  Schizo...
Q9Y7L4                63 SLFQLF-RKH-KGYLNVTDLVLPLWCEVQHEYYLLR-----RIKKKTPKMERGIKLHQILEYETsppS----------ER 125  Schizo...
Q6CHE7                71 lQLKAL-KNPgSNRVSVTAASAP-FCEVRTLYELAEVa--vEEEKPTNAMERGAQVHNMAEYSL---Y----------EA 133  Yarrow...
XP_002141959          49 vPIQKY-RD--KSQIGVTTLSRQIWCEKSLELSLEY-----DVIASSKAIDEGVRHHEILELQD---H----------DI 107  Crypto...
CCC68314             106 spfidthKSQgINRLSVTKLLTKSWCELRFTYDMYAQaglqIQPPPDKFLTSGTSIHGSLETAL---Hpgl--------- 173  Naumov...
A7TMJ6               113 nsdgngeneKvRNRLSVTKLLTKRWCELREAYDIYSE----TPLFEHKQIIEGKLVHQKLEEDI---HpvtedlesfvED 185  Vander...
GAA87462             237 VPVDI-----TTKEDALALRIWNIIQGLRMLRE-------------FGITRELEVWGL-------V-DG-----E----L 281  Asperg...
XP_011109951         325 VPVTV-----KSRHDKWGLRIWNMIQGMESLRT-------------VGLTRELEVWGW-------I-DG-----V----F 369  Dactyl...
CCX04512             187 VEVEI-----ETKEDRWGLRLFNMRQGMRSLVT-------------AGVTRELPIFGW-------FdDV----------F 231  Pyrone...
WGS:AATM:SJAG_05280  142 VRTDE-----TLAEEPWALHILLQLDGLLLLTK-------------NGITREFPIWGF-------YkDS----------L 186  Schizo...
EPY53777             126 ILLNK-----TSKEEPWALKILKQIEGLQQLYL-------------NGITREFPIWGL-------YkDS----------F 170  Schizo...
Q9Y7L4               126 RVLDR-----TSKEEPWALRLLRQLEGIMLLQK-------------NGITREFPIWGY-------YkES----------S 170  Schizo...
Q6CHE7               134 TEVSLpvppaTTPTEEWYQKLMNMYLKLSMLRTdsapavnelemspTFMAREIPVFGN-------Y-KN-----T----R 196  Yarrow...
XP_002141959         108 IDVIV-----ETSIEKLGLELLSIANLLEALYE-------------RGYVRELPIIGF-------H-KD-----I----M 152  Crypto...
CCC68314             174 --------ldWNLEHDLFDVWFDSLLKMANLFQ-------------GGQCREIIAHGYvnsndltLlDGdmdvtDeddvL 232  Naumov...
A7TMJ6               186 FEVPIp----TDNFHNHVDDLFSCSMRLLSLFR-------------CGEAREVRCHAFldsrtgtFiDGlp--kDgkdvL 246  Vander...
GAA87462             282 VTGVIDQL---SHDCPDP----ELEATAAT--------------------------------------------YYADVE 310  Asperg...
XP_011109951         370 INGVIDEV---NFTKFRNeardEKENQKVEdiddkaeelevalakndpdtiitvvgesskeipetpkkkrgrpkKNPDPV 446  Dactyl...
CCX04512             232 VFGVIDEV---SYKNPKE-------------------------------------------------------------R 247  Pyrone...
WGS:AATM:SJAG_05280  187 LFGIIDELtvlKTEKDDN-------------------------------------------------------------- 204  Schizo...
EPY53777             171 VVGIIDEI---SLAQQGT-------------------------------------------------------------- 185  Schizo...
Q9Y7L4               171 IFGIIDEI---SLNNPSK-------------------------------------------------------------- 185  Schizo...
Q6CHE7               197 LFGIMDLI---YWRNK---------------------------------------------------------------- 209  Yarrow...
XP_002141959         153 LRGIIDSL---KLIHNNNl--eMLDSLNFNnynsk--------------------------------------------- 182  Crypto...
CCC68314             233 ISGVIDHL---TLRGKDVrl----------------------------------------------------fpDVNDIG 257  Naumov...
A7TMJ6               247 VSGIIDHL---SLRRKIRvftssgftef-------------------------------------nglndevfeNGNNFQ 286  Vander...
GAA87462             311 ASRAILPEYQmslsdyllsptgggrllsevygqddtpakadgfdmdtdmnlasssqetdnfphqpRIYLTDVKTRGsgS- 389  Asperg...
XP_011109951         447 SPEPATPKQQssildfitps------------------------------------pkksrskgkPAYVLDLKTRF--Ss 488  Dactyl...
CCX04512             248 VTEVGISDTTmkgenwvfsqmegei-------------------------kvdyeayhpaekgqrEAYVSDTKTRR--Sr 300  Pyrone...
WGS:AATM:SJAG_05280  205 -----------------------------------------------------------------LLSMKDNKTRG--Ti 217  Schizo...
EPY53777             186 -----STKEHsdirdf-------------------------------------------wlpgtrNIYFTDNKTRF--Sn 215  Schizo...
Q9Y7L4               186 ------SNFNsdirny-------------------------------------------fnfkmyDLSFVDNKTRF--Ss 214  Schizo...
Q6CHE7               210 -----------------------------------------------------------------KLNICDMKTTY--Rr 222  Yarrow...
XP_002141959         183 ------------------------------------------------------------miqkyEVLITDTKTRR--Kp 200  Crypto...
CCC68314             258 ALLMELDKHRelik------------------------------------------------gdtNILVSDVKTRS--In 287  Naumov...
A7TMJ6               287 SIIEWLNANIdflk------------------------------------------------seyQINVSDVKTRM--Fr 316  Vander...
GAA87462             390 -VPTvkSSSFRPTLLQLQLYYHMLNRLVLsDD---------VT-----------------------IDLLASRY------ 430  Asperg...
XP_011109951         489 rIPEsgSSQSKSVHIQLMLYRQLLNYMIK------------AT-----------------------PKSMVRRLcl---q 530  Dactyl...
CCX04512             301 sFPT--KSQSHQTALQMMIYRHLLTDIWN-GG---------LK-----------------------FEELLKHF------ 339  Pyrone...
WGS:AATM:SJAG_05280  218 tIPS--QSQILGSELQLMYYYHLFQEYFQnADs--------------------------------wWDSFLKQQ------ 257  Schizo...
EPY53777             216 aKPS--ISQVFQSKIQVMYYAHLFLHYFP--S---------LNikstqplyatqkddnqmklekpwMDSFLNSL------ 276  Schizo...
Q9Y7L4               215 rKPG--ASQILSSKVQLMYYVHLFLNYFPsLGekqqsifrdISptysnrl----------hsqshwWNMFLSQL------ 276  Schizo...
Q6CHE7               223 sVPE--EPQMTSHIHQTMLYHYLFSDMCR-------------D-----------------------IDATMGEYlrddic 264  Yarrow...
XP_002141959         201 sTPS--IPQQKTTVFQLGLYKLLLNRMRLaGLewknkhscnINfsesydln-------eccegckfLYKLFEFN------ 265  Crypto...
CCC68314             288 rLPTq-GTVLKATKLQLMYYKFFLKSFG-------------LN-----------------------QESTYKKLlvnaqr 330  Naumov...
A7TMJ6               317 sVVSq-KSVLKYSKYQVMYYRYFLELLG-------------LH-----------------------PDVTYGQLlnssls 359  Vander...
GAA87462             431 -NLDPNRPF-----------TDAFINE-VGGLNdqffda-----mssqeFDPDyiptpqdaiehpq-------------- 478  Asperg...
XP_011109951         531 qDIPADEPF-----------SDDMIAE-LAGLDngtep------------------------------------------ 556  Dactyl...
CCX04512             340 -ELDGEAKF-----------SDGFIAQ-IAGLEtee-------------------------------------------- 362  Pyrone...
WGS:AATM:SJAG_05280  258 -DLDGKKNL-----------GQNFLKQaIETLPsi--------------------------------------------- 280  Schizo...
EPY53777             277 -NLDGSKDL-----------GTEFLEQsIRTIPti--------------------------------------------- 299  Schizo...
Q9Y7L4               277 -SLDGTKDL-----------GPKFLEQsILSIPdi--------------------------------------------- 299  Schizo...
Q6CHE7               265 iDTDVRSEF-----------SAAYEGQ----------------------------------------------------- 280  Yarrow...
XP_002141959         266 -KVDYMIQF-----------NE------INKLEisrnkenkyedsqnknIIGDyqdkniigdyqdkniiednqdkniied 327  Crypto...
CCC68314             331 rGIDVDAPIdpmkllnfmisSNGFLNDdMRKMRdgfdigfplfdnenssNNNNdaefnmsn------------------- 391  Naumov...
A7TMJ6               360 rGFNIDQRIdpakviyfmasDEVIVDD-MRKLRdgddigfppfdsdftgSSPTeeeydm--------------------- 417  Vander...
GAA87462             479 -----rhlpssqsqpnsHSSSQSSSEDST-------TILSTHNTLTTLWTL---MK---SHLRLTFLPspptaspsipse 540  Asperg...
XP_011109951         557 -------------------------------gsddlTVLLENNSIEKLYTL---YH---RRLKQTI-------------- 585  Dactyl...
CCX04512             363 ---------------------------------gflEELLRNNNLRGMMEI---LQ---KEMKEAI-------------- 389  Pyrone...
WGS:AATM:SJAG_05280  281 ----------------------------------dpKELEAHNTLSGLHALatrLT---KDFQLRS-------------- 309  Schizo...
EPY53777             300 ----------------------------------peDDFKENNSLNGLYKIsskLA---RRLRLNI-------------- 328  Schizo...
Q9Y7L4               300 ----------------------------------peDVFAGHNSLNGLYALvfaSA---KKLHLRL-------------- 328  Schizo...
Q6CHE7               281 ----------------------SGPADTI------------PTSISGLIKL---IG---DELPKPR-------------- 306  Yarrow...
XP_002141959         328 nqdkniiednqdkniieDNQDKNIIEDNQdkstiliSEILS--NFQNLLHIgfkLM---NLLAIMP-------------- 388  Crypto...
CCC68314             392 ---------ipsidsevGVNN---------------NILEKYEEFMTSWTRpvnLKyfaARMSQFYYN------------ 435  Naumov...
A7TMJ6               418 -----------silsdqITDP---------------NVLERYGEFLVPWKRpvtLKyfaARLAQMYNC------------ 459  Vander...
GAA87462             541 fqprsldpYPTILSPLLTARYISSKpsa------------dlqPPRVLGSRSFLFDPTSMSSYVADQMHWWRGERDPRGV 608  Asperg...
XP_011109951         586 ----------STIAPELTVVYRWQR------------------NGEFLDSADYMADDTYLKEHVDNVIAWWKGERATVGV 637  Dactyl...
CCX04512             390 ----------DSVGTEMGVSYLSQS------------------DGGLIGFKAIEYEENVLMEYIKESLKWWRGQRETVGV 441  Pyrone...
WGS:AATM:SJAG_05280  310 ------------LSPKLTVTYRHNI------------------SGDVIRNHIFDMNSTILNHYFHEMFAYWHNEAEPKGV 359  Schizo...
EPY53777             329 ------------SDKNLGITYWNPQ------------------TQQPLHVEKFNYEPTTLDDCYNRVNSLWRTMREPVGV 378  Schizo...
Q9Y7L4               329 ------------TDDNLTIAYRNDK------------------TGEVVYKDKFSFSNKLLEASYTKAYQFWHNLREPEGV 378  Schizo...
Q6CHE7               307 -----------SLAKTVSVEYVKRSedrvpeeyqdlyplsdytQRQSFAVVQAPVDYKELKRVMSETLPFWHGQRAAIGV 375  Yarrow...
XP_002141959         389 -----------PIHNNMKVEYDCE--------------------GCTFATKWHTCEELTIMTEVDYLLGWWLGLRDSEPV 437  Crypto...
CCC68314             436 --------IGQLISDELMVEYYHK--------------------NENFHNILFKYDNKMLKEHVTDSGKYWFGKRDIEPI 487  Naumov...
A7TMJ6               460 --------ISPLLSKHLTLEYYYK--------------------GDNFKNINFDFNEDEIRNGAFDSSMFWFGKRDIEPI 511  Vander...
GAA87462             609 EVMEA-WK--CRICDFRDDCEWR 628  Aspergillus kawachii IFO 4308
XP_011109951         638 EINET-WK--CNKCEFMEDCIWR 657  Dactylellina haptotyla CBS 200.50
CCX04512             442 EIEDA-WK--CQRCEFSEDCTWR 461  Pyronema omphalodes CBS 100304
WGS:AATM:SJAG_05280  360 PENET-FK--CKYCEFSERCWWl 379  Schizosaccharomyces japonicus yFS275
EPY53777             379 SPNEV-FK--CRSCEFREECWWl 398  Schizosaccharomyces cryophilus OY26
Q9Y7L4               379 PAEEV-YK--CRSCEFQKECWWl 398  Schizosaccharomyces pombe 972h-
Q6CHE7               376 DADSM-NK--CRYCQFRPVCGWY 395  Yarrowia lipolytica
XP_002141959         438 LNSEM-WK--CKYCNVLEYCNfs 457  Cryptosporidium muris RN66
CCC68314             488 KPNLKnFKifCKNCDYSHVCSWR 510  Naumovozyma castellii CBS 4309
A7TMJ6               512 EPTSEnLItyCKYCDYVNVCSWR 534  Vanderwaltozyma polyspora DSM 70294
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap