5HY0,4NQ3,6CWJ


Conserved Protein Domain Family
Amido_AtzD_TrzD
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pfam09663: Amido_AtzD_TrzD 
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Amidohydrolase ring-opening protein (Amido_AtzD_TrzD)
Members of this family are ring-opening amidohydrolases, including cyanuric acid amidohydrolase (CAH) (EC:3.5.2.15) (AtzD and TrzD) and barbituric acid hydrolase (BAH). Note that barbiturase does not act as defined for EC:3.5.2.1 (barbiturate + water = malonate + urea) but rather catalyzes the ring opening of barbiturase acid to ureidomalonic acid. Cyanuric acid hydrolases (AtzD) and barbiturases are homologous and found almost exclusively in bacteria. CAH is a homotetramer, the monomer of which possesses a fold that is dubbed the Toblerone fold. Although not obvious from the protein sequence, each CAH monomer possesses 3-fold rotational symmetry, for which the repeated structural motif is structurally similar to that of the YjgF superfamily of proteins (two alpha helices and a four-strand antiparallel beta-sheet), despite having low sequence similarity. This suggests that the Toblerone fold evolved from concatenated YjgF family proteins. The 3-fold symmetry of each CAH monomer extends to the active site, which contains three Arg-Lys-Ser triads, which bind the substrate via an extensive hydrogen bonding network.
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Statistics
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PSSM-Id: 401557
Aligned: 19 rows
Threshold Bit Score: 407.092
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
5HY0_A            40 MHVVPMLAPNDTAAFRALFASGAVDPASVVALIAKSEGSGLHNDHARVFADVSLRTALAEARGCPVEDLA---------- 109 Frankia sp....
CAS:pLAtcm_024     5 VHRFFSSNPADVSGLAVAIAAGNVHPQRIVAIIGKTEGNGAINDFSRGLAIMALGRLLGPFLDCSAEEIE---------- 74 
CAL79955          10 VHRLPMRSPDDVGALEAAIVSGAIDPAGIVAILGKTEGNGCVNDFTRAFAVRSLETLLGRHLQA---DAV---------- 76 
WP_015846476       6 VIKVPARSPNDTAALQRYIDESLINPKDVVAVLGKTEGNGCVNDFTRGFAVLALKNLFAS----YKEATA---------- 71  Paenibacill...
jgi:MICPUN_59838  10 VAVVDTAHPGDTGPLERWMDANPL--ADILCVLGKTEGNGCVNDFTRGYATSAIQAALGRVRSRRMGATAnddernrqss 87 
F4CUJ4             6 IVKRTTSSPDDTALVKTLADAG-YSTADVVALVAKTEGNGCVNDFSRTLADHTWDAVLP--------------------- 63  Pseudonocar...
XP_007924838      33 LIKFPTSSPADSSPLLQLSKTSDFKTDDILGIVGKTEGNGCVNDFSRTLSSHVWDEIIPSTGIS---------------- 96 
WP_015189036      14 VFRLPMSAPEDVSSLQHLLETQQIQAADIVAIIAQTEGTG----YARGYASLCLQLLLSQYLQISVEDVF---------- 79  Gloeocapsa ...
WP_013132456       7 VRKVPIESVTDASGLAKLIDEGIIEADRVLAVIGKTEGNGGVNDYTRILADRAFRDVLVQKGTRTPEEV----------- 75  Thermobispo...
A1SPN2             7 VRKVPIEHVSDAAGLADLIDAGVFSADDVIAVVGKTEGNGGVNDYTRIISTHAYRAVLEEKGTRSKEEV----------- 75  Nocardioide...

5HY0_A           110 --DSVTVAVSGGSPGVISPHVTVVTQEwvADLPAGL------PGVGLVVGRGHTEPILPEDIGRTAQVDKVADAVAAAML 181 Frankia sp....
CAS:pLAtcm_024    75 --DRIFLSLSGGTEGVTCPHYLVFTVD---ETPASS------EKKGLALAVGYTRSFAPEEAGRIPMILETARVVRALQA 143
CAL79955          77 --RQIAMVMSGGTEGALSPHMIVFEARt-GDDGPRA------FGTSLALGRARTPTLPGEHLGRMQQVAQVAAGVRAAMA 147
WP_015846476      72 --EPVSYIMSGGTEGILSPHFSVISRSesLDDAIKR------EQSSLAIGVARTPDFMPEELGRLAQVDQVAEAVRRAMD 143 Paenibacill...
jgi:MICPUN_59838  88 ftADPAIIMSGGTEGVLCPHFIVFANSwdEADADENhdddddDDAAFELHVTRTRDFKPEEVGTPVMARATRRAIEEYLG 167
F4CUJ4            64 --ADAVTVFSGGTEGVLSPHASAFVGT----DRPAA------PEGALVAAVGRTASIPIADLGRAGQVRAVAARVRELCA 131 Pseudonocar...
XP_007924838      97 -------VFSGGTEGVLSPHVAFILES--------K------QQTGLIASVSRTWKLQPWQIGTAEHVSCVSATIRKMLE 155
WP_015189036      80 --HRIPMMMIGLCGGLMSPHYTIFTRKavAAPSTPP------QEKRLAVGIADTRVLLPEEYGTMTQVELVAEAVEAAIA 151 Gloeocapsa ...
WP_013132456      76 --AQVPLVWSGGTDGVLSPHATIFATVd-PAKAPKT------DEPRLSVGVAMSEPILPEDIGRPAMIEKVANGVREAMK 146 Thermobispo...
A1SPN2            76 --AQVPLVWSGGTDGVISPHATIFAYAp-EGRYLPT------DEPRVTVGYAMSEVLLPEDIGRPAMVEKVAAGVRVAME 146 Nocardioide...

5HY0_A           182 DAGVTDPDDVHLVMVKGPALSSRAVADALSRGKTVVTGDygigPMGSMCWSNDASALGVAV----ALGEVKr--DLVADD 255 Frankia sp....
CAS:pLAtcm_024   144 QLGV-KTDDVHLVQVKGAIPAVPSVLGQSTTRE----------WRCDMAWSRGASALGVAL----ALGEVSa--DMLDDE 206
CAL79955         148 DAGIEDPADVHYVQVKCPLLTMERIEAAEARGARTAVRD----TLKSMGFSRGASALGVAV----ALGELAf--DELSDA 217
WP_015846476     144 GAGIHDAADVHFVQIKCPLLTAEQVQEANLRGASTVTED----TYKSMGYSRGASALGAAV----ALGEIQr--ERLQDT 213 Paenibacill...
jgi:MICPUN_59838 168 YGGNAPNWDDMFVQIKCPLLTPERIAEAAARGETCATTD----CYESMALSRGASSLGAMVavesALGRYSapdDARVDD 243
F4CUJ4           132 DAAL-EPGDVHLVLVKCPLLTTESISRCLADGVEPATRD----TLRSMAMSRAASALGVAV----ALGEIS---EPDAAA 199 Pseudonocar...
XP_007924838     156 TEKL-KADDVQLVLIKCPLLTSEKVAATLAAGQKPITAD----TYESMAWSRYASAIGIGA----ALGEVSm--EDVPTH 224
WP_015189036     152 QAGISSLDDVHCVEIKCPAMTLARLQDAEKRRVKVVNTN----LNQASSMAKGACALGIGL----ALKEIPq--EKLDDR 221 Gloeocapsa ...
WP_013132456     147 IAGIEDPADVHYVQTKTPLLTLETINDAKRRGHTVVTED----TLKSMDISNSTTALGIAV----ALGEI----EMPRED 214 Thermobispo...
A1SPN2           147 RAGITDPADVHYVQTKTPLLVQDTINDAERRGETVYTHN----TLESMDVSNATTALGIAV----ALGEI----EMPTAE 214 Nocardioide...

5HY0_A           256 RI----RSDWDLFSAVAATSSGGEKRGGEVLLL------ANSAQSASELRIGH-----------GITRDMADTEGIKTAI 314 Frankia sp....
CAS:pLAtcm_024   207 MI----NRDWTQFSTRASVSAKPALQRSEICLV------ANAAGWSKDFQIAH-----------GTMRDILDVSAIYKVL 265
CAL79955         218 DI----CADYSRYSERAATSGGVELLDHEIMVA------GMSARWTGPLAIDH-----------GVMRDAIDIEPARAAL 276
WP_015846476     214 DI----CHSWELYSKVASTSAGSELAYCEVIVF------GNSAYAEGPYFIDH-----------DVMKDAIDAGALKRIL 272 Paenibacill...
jgi:MICPUN_59838 244 AVyaktCEDYSVFSDHVSSSAGVELMHCEILMMltrrrePSSKTPRSKFRMAR-----------GRMDDAIDLAGVTRCV 312
F4CUJ4           200 AL----RGEADVWSSVASISSGAELDDCHILVL------GNSPAAHGPLRAVH-----------GVMRDAMDARTVLDLL 258 Pseudonocar...
XP_007924838     225 ------LHSGNFYTAKASCSSGAELDDCHILIL------ANDP--SIDVEAEHgdpppliqgfcGVMSSAVDFAGLQEVL 290
WP_015189036     222 AI----NSNHALYTNRGSVSAGGEQSACRVLVM------GNSAFSASRYRIGS-----------GVMQNQLDTTGVYAAL 280 Gloeocapsa ...
WP_013132456     215 QI----HRDLSLYSSVASCSSGVELDRAQIVVM------GNVRGIGGRYRIGH-----------SVMKDALDTDGIWEAI 273 Thermobispo...
A1SPN2           215 QI----FHDLSLYSSVASCSSGVELDQAQIVVV------GNARGVGGRFRVGH-----------SIMKDALDMDGVWAAI 273 Nocardioide...

5HY0_A           315 RTAGVDFDc---cLSPAQQAQVVQVFGKFVLPGSDVLRG-QHITALDDHEAHHV---AKAVGGALVVSITGQPMSFISGG 387 Frankia sp....
CAS:pLAtcm_024   266 EDLELRPVcg--qLPREQAARVYGVFAKSDADPRGTVRG-RRHTMWTDGDISDMr-ySRCVVSSLLAGILGESAVYVSTR 341
CAL79955         277 SRLGLDGPg---qLPAASRGRIAAVLAKAEAAQSGKVRG-LRHTMLDDSDVSSTr-hARAFVGGALAGLFGFTDLFVSGG 351
WP_015846476     273 E--------------KYPDAEMVQVLAKAEADPTGYIRG-QRHTMLDDSDINHTr-hARAVVGGVLASVCGDPMIYVSGG 336 Paenibacill...
jgi:MICPUN_59838 313 D------------AARGSRGTNTIVTAIVKADPAASVRG-RRTTMLSDSDIHATr-hARAAVGGVVAAAVGDCRVYVSGG 378
F4CUJ4           259 D------------RVSADGGEVVQVLAKAEADPSGSIRG-RRHTMLTDSDLSSTr-hARAAVGGLLAGLVGDSAIYVSGG 324 Pseudonocar...
XP_007924838     291 NRASRP-------KAPYRKAKLLQVFAKAEADPTGMVAGtYRHTMLTDSDLHSTr-hARAAVGGLIAGVTGDCHIYVSGG 362
WP_015189036     281 KSAGLNCQi---pLTTDQQNKITQVFVNCGADAVTAVGD-RRHTMHSDFLAGYAgimAKAVANAIVASVVGDTMILASAG 356 Gloeocapsa ...
WP_013132456     274 RNAGLDLPer--pHPSDLKGRLVNVFLKCEADPTGRVRG-RRNIMLDDSDVHWHr-qIKAAVGGVAASVTGDPAVFVSVA 349 Thermobispo...
A1SPN2           274 RDAGLDdmpvdciHPRHIKGRLVNLFLKCEADPTGRVRG-RRNIMLDDSDVAWHr-qIKACVGGVVAAVSGDPMNFVSVA 351 Nocardioide...

5HY0_A           388 ErnSHMGPPGGNPVAAVVR 406 Frankia sp. Eul1b
CAS:pLAtcm_024   342 A--EHQGPLGGGPVAIIAR 358
CAL79955         352 A--EHQGPDGGGPVAIIVE 368
WP_015846476     337 A--EHQGPAGGGPVAVVFR 353 Paenibacillus sp. JDR-2
jgi:MICPUN_59838 379 A--EHQGPDGGGPVCVISR 395
F4CUJ4           325 A--EHQGPPGGGPVTVVYR 341 Pseudonocardia dioxanivorans CB1190
XP_007924838     363 A--EGQGPKGGGPVCVVAR 379
WP_015189036     357 N--EHQGARGSNLVAAIVD 373 Gloeocapsa sp. PCC 7428
WP_013132456     350 A--VHQGPAGGGPVIAIVD 366 Thermobispora bispora
A1SPN2           352 A--VHQGPSGGGPVIAIVD 368 Nocardioides sp. JS614
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