Conserved Protein Domain Family
Afi1

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pfam07792: Afi1 
Docking domain of Afi1 for Arf3 in vesicle trafficking
This domain occurs at the N-terminal of Afi1, an Arf3p-interacting protein, is a protein necessary for vesicle trafficking in yeast. This domain is the interacting region of the protein which binds to Arf3, the highly conserved small GTPases (ADP-ribosylation factors). Afi1 is distributed asymmetrically at the plasma membrane and is required for polarized distribution of Arf3 but not of an Arf3 guanine nucleotide-exchange factor, Yel1p. However, Afi1 is not required for targeting of Arf3 or Yel1p to the plasma membrane. Afi1 functions as an Arf3 polarization-specific adapter and participates in development of polarity. Although Arf3 is the homolog of human Arf6 it does not function in the same way, not being necessary for endocytosis or for mating factor receptor internalization. In the S phase, however, it is concentrated at the plasma membrane of the emerging bud. Because of its polarized localization and its critical function in the normal budding pattern of yeast, Arf3 is probably a regulator of vesicle trafficking, which is important for polarized growth.
Statistics
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PSSM-Id: 400236
View PSSM: pfam07792
Aligned: 24 rows
Threshold Bit Score: 79.423
Threshold Setting Gi: 1051433413
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
KQC41027               29 YIISAEFDNKLGPILKHQYPKDIPGfnqfsheqrngnTSVSmnLASLMIPSSIERNPGk-qDITVFTLYYNKFTQNYQLF 107  Sacch...
Q75BQ9                 52 YILAAEFDNKLGPVLKHQFPKNLPGfk--------stNSNLsnLAGLMIPNGIENRPGe-sDFTTFILYKDRYAQTFQLF 122  Eremo...
CAR24421               32 YIISAEFDNKVGPVIKHQHPKPLAGfks------stsRSASvnLASLMIPNSAESRPDv-aDFTVFILYKDKYTRNYHVF 104  Lacha...
Q6CPS0                 31 FIIAAEFDNKSGPIIKHQYPKTIPGfkt------igsDSTCshLASLMIPNSVENHPGk-pDFTVFVLYKNQNV--YQLF 101  Kluyv...
CAR29254               11 YLLTAEFDNRFGPVVRDQYPSVIPGfeyemr-eghsqNNAVfnLASLMIPNNAEYNTGdepDTTVFMLYRNRETVKYELF 89   Zygos...
XP_001643016           76 FILSAEFDNKLGPKVKFQYPNSIPGfpsslh-gahslDSTIn-LASLMIPNNVEYTPGk-kDFTVFMLYYNTSTKLYQLF 152  Vande...
LoveMIT:GQ68_03095T0    9 YILTAEFDKSKGIVITNQHPCEdds---------wllSSQLqdISNVLMPSQLQHSMNr-eSITVFPIYSTYSDVEFE-- 76   Komag...
Q23F93                  4 VIFCSEFDLLKGCVVKAHYPENfe------------yDEIT--LAAYMLPDGVQKFDN---DISVF-------------- 52   Tetra...
EFC40537               63 YIFLAEFDIDKGSIVKVQYPSPYP-------------YATEysIANLMLPDGSHNCIE---DTTVFFLRGNNEINNNHHN 126  Naegl...
XP_002615806            6 FIVTAEFHVDKGPSLIHQYPNVLPG------------SEGLqyLPELMLPDQIHKRPE---DYTLFLLYVNSL------- 63   Clavi...
KQC41027              108 pvpkdprfsfnlhhreqsdgsvtnsiYYDAENHQDAKNNRyTIVLEDDELEc-----------QEVQNnqnAIDNE-PL- 174  Sacch...
Q75BQ9                123 pndnakgslkpt---aeshghlggspSSRLES----------MILEEDENAgsskt-------------slsqAREiPL- 175  Eremo...
CAR24421              105 piad------------------sklpQNDARS---------PTILEEDEPQsg--------laGEASTnhnADTKEpPL- 148  Lacha...
Q6CPS0                102 pvsk------------------nktkGTDLDS---------SLIIEEEDFEeeqenglrsgkiGSIRDdrhLEETEpPL- 153  Kluyv...
CAR29254               90 p-------------------------SKQVDQV---------------------------------------------I- 98   Zygos...
XP_001643016          153 k------------------------pSNIKTEVQKNDNDDnDDEENEDGDGdddseyidseifTDAKDendNLDDIdTL- 207  Vande...
LoveMIT:GQ68_03095T0   77 --------------------------NAILSYDPKPHSLp---------------------------------------s 91   Komag...
Q23F93                 53 --------------------------KYKLKQVVDQKTH-------------------------KLVSeinLNSSKeLV- 80   Tetra...
EFC40537              127 enntsasslnnsnsgnnnssilsngsNDGGEEAKSSPRN-----SQEVVEKvdlntslestilSSDQViteQSKKEeIFy 201  Naegl...
XP_002615806           64 --------------------------TGKLQYQYDSKTC----------------------------------DSKp-F- 81   Clavi...
KQC41027              175 FFINVANTVLDTTN 188  Saccharomyces sp. 'boulardii'
Q75BQ9                176 FFINVVHAKIDNAN 189  Eremothecium gossypii
CAR24421              149 FFLSVVNALHDKSN 162  Lachancea thermotolerans CBS 6340
Q6CPS0                154 FFLSVVNTFLDNSN 167  Kluyveromyces lactis
CAR29254               99 CFVNVVYAQEDKSN 112  Zygosaccharomyces rouxii
XP_001643016          208 YFINVVNTVIDKNN 221  Vanderwaltozyma polyspora DSM 70294
LoveMIT:GQ68_03095T0   92 VILSISYFKSDDSY 105  Komagataella phaffii GS115
Q23F93                 81 DLYKFNNQKDDWEI 94   Tetrahymena thermophila SB210
EFC40537              202 YVLNVQKQKKYEGL 215  Naegleria gruberi
XP_002615806           82 FVYTIVNNVPDQNV 95   Clavispora lusitaniae ATCC 42720
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