Conserved Protein Domain Family
SARAF

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pfam06682: SARAF 
SOCE-associated regulatory factor of calcium homoeostasis
SARAF is as family of eukaryotic proteins embedded in the ER. SARAF is SOCE-associated regulatory factor, where SOCE is store operated calcium entry. ie a mechanism governing calcium homoeostasis in the cell and the mitochondria. SOCE involves the enabling of Ca2+ release-activated Ca2+ (CRAC) channels. SARAF is a single pass ER membrane protein whose systolic-facing domain is responsible for activity and whose luminary-facing domain carries out a regulatory function in conjunction with another membrane protein STIM, an ER single pass membrane protein that detects changes in ER Ca2+ levels through its EF-hand, conserved Ca2+ binding domain. STIM is the major target for SARAF regulation, and thus SARAF negatively regulates the SOCE entry of calcium into cells protecting them from overfilling.
Statistics
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PSSM-Id: 399577
View PSSM: pfam06682
Aligned: 47 rows
Threshold Bit Score: 236.46
Threshold Setting Gi: 526196225
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EGF82781      18 LLFLVCILAPTATAAShkKVRLTNIQALTLHAGRMTTARRSSPVKQLSCVnsgnsgffSssKSACSDTallPQTVQCINK 97  Batrachochytriu...
EGV98445      19 LLSFLLVAGPALCWNDpdRILLRDVKALTLHHDRYTTSRRLDPIPQLKCVg-------GtaGCDAYT----PKVIQCQNK 87  Chinese hamster
XP_006010005   8 LFVLSLMLGPADGWNSheSVLLQEVQVLTLYKNRFTTARRTSPVPQLQCNg-------GtaGCSAFV----SEVVQCQNK 76  coelacanth
XP_015199412   7 IFVLFIYTGRTESWNDggSVLLRELQVITLYKGQYTNSRRTSPVPQLQCIg-------GtaGCGSFI----PEVVQCHNK 75  spotted gar
F1QPC3         9 LQFLLLVVPHISCWNDe-AVLLRDVQVLTLYRGRYTTARRSSPVPQLQCIg-------GsaGCGSFT----PEVVQCYNR 76  zebrafish
XP_005810431   8 VVLLFVLVETTYSWNQd-SVLLRDIQALTFYRNRFTTARRSNPVPQLKCVg-------GsaGCHVFI----PDVVQCVNR 75  southern platyfish
XP_011616782   9 WLFLLVEITSSLNGD---AVLLQDVQVLTLYRGLYTTARRSSPVPQLQCVg-------GsaGCHAFV----PEVVQCQNK 74  torafugu
4_pfamImport   1 LQFLL--VGHIKSWDDg-SVLLRDLQALTLYKNRYTTARRASPVPQLQCVg-------GsaGCQAFV----PEVVQCQNK 66 
F7C1E2        14 pLLALLQIPGAWCWNHqeRVLLRDIQAITLYADRYTNARRSAPVPQLKCIg-------GsaGCHTMV----PQVVQCHNR 82  tropical clawed...
ESO08194       5 VFFVCFLATILACVSGhkKVLLKDVQVVTLRNKEYTTGRRNKPIPQLHCLg-------GfsGCE-TVT---LDTVQCYNR 73  Helobdella robusta
EGF82781      98 GTDGVDVQWACTAELDSTVKFGSeMVVACEGWAYPEDPFVLAGSCGLEYTL-VRTALY----------DRTRPSKEHHRF 166 Batrachochytriu...
EGV98445      88 GWDGYDVQWECKTDLDIAYQFGK-TVVSCEGYESSEDQYVLRGSCGLEYHL-DYTELG----------LKKMKESAKHQg 155 Chinese hamster
XP_006010005  77 GWDGFDVQWECKTDMDNNYRFGT-IQVNCEGYNSPDDPYVLKGSCGLEYTL-ELTEAG----------RMKRNKGYGSGG 144 coelacanth
XP_015199412  76 GWDGFDVQWECKTDMDYSYRFGK-IEVSCEGYNYPDDPYVLKGSCGLEYTL-ELTEEG----------KQKGRNSRSfgs 143 spotted gar
F1QPC3        77 GSDGIDAQWECKADMDNLYRFGR-VEVSCEGYNSPNDAYVLRGSCGLEYTL-ELTAEG----------KQQQGSSGFSGf 144 zebrafish
XP_005810431  76 GWDGVDVQWECKTDMDNAYRFGA-IEVSCEGYSHPSDDYILKGSCGLEFTL-ELTQEG----------QRRTQGSFGSQG 143 southern platyfish
XP_011616782  75 GWDGMDVQWECRTDMDNAYRFGR-IEVSCEGYNHPADAYILKGSCGLEYTL-ELTREG----------RGKHTGSHSGFS 142 torafugu
4_pfamImport  67 GWDGVDVQWECRTDMDNAYRFGR-IEVSCEGYSHPADALILKGSCGLEYTL-ELTEHG----------RSTSHGGTKSFG 134
F7C1E2        83 GWDGFDVQWECKVDMDNSYRFGK-VEVSCEGFDYPEDPYVLRGSCGLEYTL-ELTEEG----------RKRSQDGYRSGG 150 tropical clawed...
ESO08194      74 GFDGNDYNWECKADMPKHFKFGK-MEVTCEGYDYPDDPYILAGSCGLRYELiDLSDKSglfspppdayEKKYSSPTSDYK 152 Helobdella robusta
EGF82781     167 GEKWKpswsfwksk------------------ehyytentDGSSWIGFI-I-WTAVFAFIAYRISLACC-----Sk--PR 219 Batrachochytriu...
EGV98445     156 fsdyy-----------------------------hklyssDSC-GISGL-V-TIGVLLAVAYVVYKLFLsd-gqAs--LP 200 Chinese hamster
XP_006010005 145 fssnffkd------------------------fsnnvnspVSSDSSGVL---VVVILLVIAYGIYKIFLss--------- 188 coelacanth
XP_015199412 144 gffqd---------------------------sssnvnyqGSGGDVSGL-M-VVAVLLLLAYGVYKMFLcgp-------- 186 spotted gar
F1QPC3       145 asnffqgfsnqkqqqrg------gggdgggpyqqhsyqnsPSGDGVGGL-L-VVAFLLLVAFVVYKMFLcs--------- 207 zebrafish
XP_005810431 144 GFKQWgglgglassffssffgnrhhqhqqhqqqhqqqhyaQDGESSGGL-L-VVAVLLLIAYGLYKLFL----------- 210 southern platyfish
XP_011616782 143 AFGDLggfassffgssgnk--------hqqnqhnrhssdpSANGNFGNL-L-VVAVLLLMAYGVYKLFLse------niP 206 torafugu
4_pfamImport 135 GRRid---------------------------------daKSR-----L-L-VVAVLLLVAYGVYKLFL----------- 163
F7C1E2       151 fgsgyfqs------------------------nsnswdtkTNGSSA----I-VLIVIVIIAYGVYKLFLsg--------- 192 tropical clawed...
ESO08194     153 NRHYTekt-----------------------------kakDSSSETSPLfIfCIFVGIIVVVLWYSKSRqqaptSqasDP 203 Helobdella robusta
EGF82781     220 RQGNHRTTETRGTFHSTahagt--------sfdpnSGPPPPYndgwesqdn---------thqhasaphPGAYPSSGGGn 282 Batrachochytriu...
EGV98445     201 PY-------SEYPPYsqryq----------rssyaAGAPQPGfkseftgpqn--------tgfgsaftgQGC-------- 247 Chinese hamster
XP_006010005 189 --------pQSEQPFPGstgdfntdrqnyyssshsMGPSPPGfkpdfaghstd------gegfsggfnfGNYD------- 247 coelacanth
XP_015199412 187 -------hgQGHQRFPDadfsnantd-ghftdgntMGPPPPGfksyytgespgyesardsygfkntftrPHQ-------- 250 spotted gar
F1QPC3       208 -------ptNGHQGFGGdqgydstn--gawdnsqgMGPPPPGfkpdyysgdhsns--rpgygfsdsytrPQSSWTGFTGr 276 zebrafish
XP_005810431 211 ---------SGDTVQWGqtggqpgyp-hnnhsnnmGGPPPPGfkpdytgfpgtn----pgygfhndytqGQY------Yq 270 southern platyfish
XP_011616782 207 NLGQDGYHGHQDHGSTAappppgf----kpdftgySGPNPGYgf-----------------------rdDFTRSQQ---- 255 torafugu
4_pfamImport 164 ---------SGEPRPPWgrtvdrqvtpgiiahgstAAPPPPGfkpdftgnpgsn----pgygfhsdytdRQQ-------- 222
F7C1E2       193 -------psVQEHPRPDeygydf------qsqtraSAPPPPGfksdftdphlcki-fgasksfssgygtSHS-------- 250 tropical clawed...
ESO08194     204 PAPPPYGFRASPPPYDDhv--------------gsQQPPPPGfntgfspppfqs---ppsygfktnagnQQQQQQQ---- 262 Helobdella robusta
EGF82781     283 SQNSdGSRSNNDGFWSGFTSGSILGSLFGNRSYWYPSTNYFGYGpqaypsytfypprptswwswGSGWPqst-------- 354 Batrachochytriu...
EGV98445     248 ------DNSG-PGFWTGLGAGGLLGYLFGSNRA---ATPF------------------------SDSWYypsyppsypgt 293 Chinese hamster
XP_006010005 248 TRHRhNGNSG-PGFWTGLGTGGILGYMFGNQRSN--QSPY------------------------TNIWGnpaypppmhgy 300 coelacanth
XP_015199412 251 -----KPASG-PGFWTGMGTGGVLGYLFGSQR------------------------------------------------ 276 spotted gar
F1QPC3       277 TRPGgGGGGG-GGFWTGMGTGGILGYLFGNQRRQ---------------------------------------------- 309 zebrafish
XP_005810431 271 GRRSgPGTGGGGGFWTGMGTGGMLGYLFGRQRTqpcsppppgq------------------------------------- 313 southern platyfish
XP_011616782 256 YPGGrSAHSGGGGFWTGMGTGGVLGYLFGRQRSqs--------------------------------------------- 290 torafugu
4_pfamImport 223 YPGGhATPGTGGGFWTGMGTGGMLGYLFGHNRSVKRERERRIHDynle-------------------------------- 270
F7C1E2       251 -------NQG-PGFWTGLGTGGVLGYLFGNRRAQPYQAPY------------------------FNTWTgpsnsdsmh-- 296 tropical clawed...
ESO08194     263 QQQPrQQSSRIGGFWTGASLGGLAGYLFGSRRNSNNYGGRDYYDsgssyfggs---ryrnvgsrGDGWFg---------- 329 Helobdella robusta
EGF82781     355 --ytYSnpmfrrtssssgsrsqspaPSTRTSTTYGGTRRR 392 Batrachochytrium dendrobatidis JAM81
EGV98445     294 wnsrAYsplgggagsysassslnadSRTRTASGYGGTRRR 333 Chinese hamster
XP_006010005 301 ---------------psanrgvsesTGTRTASGFGGTKRR 325 coelacanth
XP_015199412 277 ----SQpryftrntgnsnigptpvnSGTRTVSGFGGTKRR 312 spotted gar
F1QPC3       310 ----PPmgfagptynsnpspapktsSGTRTASGFGTTKRR 345 zebrafish
XP_005810431 314 ----------------nhssgtntsSGTRTASGFGGTKRR 337 southern platyfish
XP_011616782 291 ---hGYqyssyhdntasnngthsssTGTRTASGFGGTKRR 327 torafugu
4_pfamImport 271 -knrANnydhspysrhppaagytssPGTRTASGFGGTKRR 309
F7C1E2       297 -----------------qnyrppqsSGVRTASGFGGTKRR 319 tropical clawed frog
ESO08194     330 ----SGssgwgsaagsttttttssaPNTETRSGFAETKRR 365 Helobdella robusta
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