7EXF


Conserved Protein Domain Family
Raffinose_syn
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pfam05691: Raffinose_syn (This model is not part of the current CDD release)
Raffinose synthase or seed imbibition protein Sip1
This family consists of several raffinose synthase proteins, also known as seed imbibition (Sip1) proteins. Raffinose (O-alpha- D-galactopyranosyl- (1-->6)- O-alpha- D-glucopyranosyl-(1<-->2)- O-beta- D-fructofuranoside) is a widespread oligosaccharide in plant seeds and other tissues. Raffinose synthase (EC:2.4.1.82) is the key enzyme that channels sucrose into the raffinose oligosaccharide pathway. Raffinose family oligosaccharides (RFOs) are ubiquitous in plant seeds and are thought to play critical roles in the acquisition of tolerance to desiccation and seed longevity. Raffinose synthases are alkaline alpha-galactosidases and are solely responsible for RFO breakdown in germinating maize seeds, whereas acidic galactosidases appear to have other functions. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36C.
Links
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Statistics
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PSSM-Id: 516928
Aligned: 5 rows
Threshold Bit Score: 1258.59
Created: 14-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
7EXF_B         7 VRISDGnLIIKN-RTILTGVPDNVI----TTSASEA------------GPVEGVFVGAVFN-KEESKHIVPIGTLRNSRF 68  thale cress
Q9SYJ4        34 FNLSEGsLCAKDsTPILFDVPQNVTftpfSSHSISTdaplpillrvqaNAHKGGFLGFTKE-SPSDRLTNSLGRFEDREF 112 thale cress
Q8RX87         7 VRISDGnLIIKN-RTILTGVPDNVI----TTSASEA------------GPVEGVFVGAVFN-KEESKHIVPIGTLRNSRF 68  thale cress
XP_009116453   7 ISVQNDnLVVQG-KTILTKIPDNII----LTPVTGA------------GSDSGAFIGATFE-QSKSLHVFPIGVLEGLRF 68 
Q9FND9        22 FRLEDS-TLLANgQVVLTDVPVNVT----LTSSPYLvdkd----gvplDVSAGSFIGFNLDgEPKSHHVASIGKLKNIRF 92  thale cress

7EXF_B        69 MSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNdgshlesdgangvecNQKVYTVFLPLIEGSFRSCLQGNVNDEVELC 148 thale cress
Q9SYJ4       113 LSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIP---------------EIDSYVAIIPTIEGAFRASLTPGEKGNVLIC 177 thale cress
Q8RX87        69 MSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNdgshlesdgangvecNQKVYTVFLPLIEGSFRSCLQGNVNDEVELC 148 thale cress
XP_009116453  69 MCCFRFKLWWMTQRMGTCGKDIPLETQFMLLESKdevn--------gddAPTVYTVFLPLLEGQFRAVLQGNEKNEIEIC 140
Q9FND9        93 MSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSgsdsg------pgsgSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVC 166 thale cress

7EXF_B       149 LESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLK 228 thale cress
Q9SYJ4       178 AESGSTKVKESSFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVK 257 thale cress
Q8RX87       149 LESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLK 228 thale cress
XP_009116453 141 LESGDKAVETSQGTHLVYVHAGTNPFEVITQSVKAAERHMQTFHHREKKKLPSFVDWFGWCTWDAFYTDVTAEGVDEGLR 220
Q9FND9       167 VESGSTEVTGSEFRQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVK 246 thale cress

7EXF_B       229 SLAAGGTPPKFVIIDDGWQSVERDAT---V-EAGDEKKESPIF--RLTGIKENEKFKKKD-------------------- 282 thale cress
Q9SYJ4       258 EFEDGGVCPKFVIIDDGWQSINFDGDeldK-DAENLVLGGEQMtaRLTSFKECKKFRNYKggsfitsdashfnplkpkml 336 thale cress
Q8RX87       229 SLAAGGTPPKFVIIDDGWQSVERDAT---V-EAGDEKKESPIF--RLTGIKENEKFKKKD-------------------- 282 thale cress
XP_009116453 221 SLSEGGTPPRFLIIDDGWQQIENKEK-----DSNCVVQEGAQFatRLVGIKENAKFQKSDpk------------------ 277
Q9FND9       247 CLVDGGCPPGLVLIDDGWQSIGHDSDg-iDvEGMNITVAGEQMpcRLLKFEENHKFKDYVspk----------------- 308 thale cress

7EXF_B       283 ---------------------------------------------------------DpnVGIKNIVKIAKEKH-GLRYV 304 thale cress
Q9SYJ4       337 iykateriqaiilrrklvkesgeqdlteldekikilseelnamfdevekeeslgsddVsgSGMAAFTKDLRLRFkSLDDI 416 thale cress
Q8RX87       283 ---------------------------------------------------------DpnVGIKNIVKIAKEKH-GLKYV 304 thale cress
XP_009116453 278 --------------------------------------------------------dTqvSGLKSVVDNAKQRH-NVKQV 300
Q9FND9       309 ------------------------------------------------------dqnD--VGMKAFVRDLKDEFsTVDYI 332 thale cress

7EXF_B       305 YVWHAITGYWGGVRP----GEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGV 380 thale cress
Q9SYJ4       417 YVWHALCGAWNGVRPe--tMMDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGA 494 thale cress
Q8RX87       305 YVWHAITGYWGGVRP----GEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGV 380 thale cress
XP_009116453 301 YAWHALAGYWGGVKPaasgMEHYDSALAYPVQSPGVLGNQPDIVMDSLAVHGLGLVNPKKVFNFYNELHSYLASCGIDGV 380
Q9FND9       333 YVWHALCGYWGGLRPe--aPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGV 410 thale cress

7EXF_B       381 KVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVS---- 455 thale cress
Q9SYJ4       495 KIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLaTKQISIGRVGDDFWWQDPYGdpqg 574 thale cress
Q8RX87       381 KVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVS---- 455 thale cress
XP_009116453 381 KVDVQNIIETLGAGLGGRVSLTRSYHQALEASIARNFKDNGCISCMCHNTDGLYS-AKQTAIVRASDDYYPRDPAS---- 455
Q9FND9       411 KVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLgTEAISLGRVGDDFWCTDPSGdpng 490 thale cress

7EXF_B       456 ----HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPG--KHNFELLRKLVLPDGSILRA 529 thale cress
Q9SYJ4       575 vywlQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGkaSHNFDLIKKLAFFDGTIPRC 654 thale cress
Q8RX87       456 ----HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPG--KHNFELLRKLVLPDGSILRA 529 thale cress
XP_009116453 456 ----HTIHIASVAYNTLFLGEFMQPDWDMFHSLHPTAEYHAAARAVGGCAIYVSDKPG--NHNFDLLRKLVLPDGSVLRA 529
Q9FND9       491 tfwlQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAASRAISGGPIYISDCVG--KHDFDLLKRLVLPNGSILRC 568 thale cress

7EXF_B       530 RLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTD 609 thale cress
Q9SYJ4       655 VHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA 734 thale cress
Q8RX87       530 RLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTD 609 thale cress
XP_009116453 530 RLPGRPTRDCLFADPARDGTSLLKIWNMNKFTGIVGVFNCQGAGWCKDTKKNRIHDTSPGTLTGLVRAEDADLISEVAG- 608
Q9FND9       569 EYYALPTRDRLFEDPLHDGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPI 648 thale cress

7EXF_B       610 --PTTWNGDCAVYSQSRGELIVMPY-NVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAE 685 thale cress
Q9SYJ4       735 gsQVTYTGDYLVYKQQSEEILFMNSkSEAMKITLEPSAFDLLSFVPVTELVSsGVRFAPLGLINMFNCVGTVQDMKVTGD 814 thale cress
Q8RX87       610 --PTTWNGDCAVYSQSRGELIVMPY-NVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAE 685 thale cress
XP_009116453 609 ---EDWSGDSIVYAYKSGELVRLPk-GASIPLTLKVLEYELFHISPLKEITA-NISFAPIGLLDMFNSSGAIQSMEINTV 683
Q9FND9       649 --SIANVEEFALFLSQSKKLLLSGL-NDDLELTLEPFKFELITVSPVVTIEGnSVRFAPIGLVNMLNTSGAIRSLVYNDE 725 thale cress

7EXF_B       686 k------------------mKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYdSSsGLVTFEL 732 thale cress
Q9SYJ4       815 n-------------------SIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWeEEtGKLSFFV 860 thale cress
Q8RX87       686 k------------------mKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYdSSsGLVTFEL 732 thale cress
XP_009116453 684 tddkpevsssvvsenrsptaLISLGVRGCGRFGAYSSQRPLRCAVDGTETEFNHdAEvGLVTLNl 748
Q9FND9       726 --------------------SVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGY-ED-SMVMVQV 768 thale cress
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