Conserved Protein Domain Family
MAD

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pfam05557: MAD 
Mitotic checkpoint protein
This family consists of several eukaryotic mitotic checkpoint (Mitotic arrest deficient or MAD) proteins. The mitotic spindle checkpoint monitors proper attachment of the bipolar spindle to the kinetochores of aligned sister chromatids and causes a cell cycle arrest in prometaphase when failures occur. Multiple components of the mitotic spindle checkpoint have been identified in yeast and higher eukaryotes. In S.cerevisiae, the existence of a Mad1-dependent complex containing Mad2, Mad3, Bub3 and Cdc20 has been demonstrated.
Statistics
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PSSM-Id: 487826
Aligned: 13 rows
Threshold Bit Score: 526.605
Created: 14-Feb-2025
Updated: 28-Apr-2025
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
NP_957206     53 AAERVRSHTSLIQLTQEKQQMELSHKRARIELEKEahsssrdLQREIDRNRDLQMKIRRLEERE-EKANQALNEQMENNK 131
P87169        54 NPKLASLEFQLENLKNDLKRKELEFEREQIELQRK-------LAEEHEQKNSLQLRLTLVEKQL-EEQSTSYQKEIEEVR 125 Schizosaccharom...
XP_013025869  41 SSRVQSLEFALENAKNDLKQKEIESDRERNDLLAK-------LTEEREQSKAFQIRVSLLEKQE-HEREATNHETIERLR 112
XP_002063744  83 RAELIESKAIVSQLQRDMEREAKEYRSELAISESKaaalkeqCDYTSAKMQELEKHLQVVRKREqVASESASRANVELAQ 162
XP_002050330  85 RAELIESKAIVSQLQDQLAQQAKEQRSALALAESKatalkqqCDYTSAKLLEQDKQLQVVRKREhNARQEANRAMAEMAQ 164
Q7KND8        80 RTELIETKAIVIQLRNEIEKKSREHKEAILLAENKstalkdqCDITSKKNLELQDDLKALRKRElVLKNEASRATAELNQ 159 fruit fly
XP_001959874  79 RADIIELKARNSQLQHEVERQIKEHRRAMAVAESKstalkqqCDITSKKMIEMERNVQIMRKREvALKEEACSARAELAQ 158
XP_002018198  82 RAELIETKARLSQVQDQLKQNETKRRMELSLAESKvsalktqCDFTSQKLLDLTKDMENRRGLEnSYKEEARRAKAELAG 161
XP_005054501  54 QAGQIHSKSQLLQVEREKMQMELSHKRARIELEKAantnarnYEREADRNQELLTRIKQYQERE-AEAENKLKEQMEMNK 132
Q9WTX8        54 RAEQIRSKSYLIQVEREKMQMELSHKRARVELERAastnarnYEREVDRNQELLARIRQLQECE-ATAEEKMREQLERHR 132 house mouse
NP_001095514  54 RAEQIRSKSHLIQVEREKMQMELSHKRARVELERAastsarnYEREVDRNQELLTRIRQLQERE-AEAQEKMKEQLERHR 132
XP_006637049  53 EAQKIFSKTHLLQMAQEKSQMEFSHKRARIELEKAahtsardLEREVDRNQDLLARIRKLEEKE-SEAGKALKEQTEISR 131
1_pfamImport   1 AAEKVQSKNRYLQLDQEKRQMELSHKRARYELERAasdsardFEHEVDRNQDLLGRIKKLEERE-TEEAKNLSEQVEVNR 79 
NP_957206    132 alkrnleelhkkaNEKDSKLTEAEQIISELKDETRRLGQQIQIQESKFSTQNLEIQALQEQLDVQRKKFQDISQKY---- 207
P87169       126 -------------NEKEATQVKIHELLDAKWKEIAELKTQIEKNDQALSEKNHEVMVSNQALQMKDTNLTNLEKLFadsr 192 Schizosaccharom...
XP_013025869 113 -------------SDNESLVLQLSELKSNLTQETAARATELEKTQQELREKIYKVEDFQQRNKLLEENASQMEIRNsslq 179
XP_002063744 163 qktkyeaiinqleKTKMQQEGDARDVHLCIGNELNEYRRLAQRANLELQSTRNELERIQRRHDEYKARISNYEELK---- 238
XP_002050330 165 qklkyetalsraeQEKTQLEEDAREVQHCINNELSEYKRISERAELELEATQSELERIRERHDEYKATAANYEQLQ---- 240
Q7KND8       160 lrlkfdestlklqKEKYLQKEDARDVHLCINNELSEYRRIAQRADLELQSTRNELERLRQLNEELQARASGFEQLR---- 235 fruit fly
XP_001959874 159 lkmkfddsidslqKEKSQQEEDARDVQLCINNDLAEYRRMAHRADLELQATRTELERFRRRHDEFKARVSGHEDLR---- 234
XP_002018198 162 lklkydetvnklkNEKSQQEQDARDVQLCINNELGDYRRQAQRAELELQSTLKELECIRQRHDEYKARMSGYEELR---- 237
XP_005054501 133 sykksmetmskkiQEKESKLAEANETITVLKGKISELQWNIMNQEMQMTSQDSEKQELMEQLDIEKKKWQEASQQI---- 208
Q9WTX8       133 lckqnldavsqqlREQEDSLASAREMISSLKGRVSELQLSAMDQKVQVKRLESEKQELKEQLELQQRKWQEANQKI---- 208 house mouse
NP_001095514 133 lcqqsldaagrklREKEDGLAEAGETITTLKGRLSDLQWSMMNQEMQVKRLESEKQELKEQLDLQHQKWQEANQKV---- 208
XP_006637049 132 slkktiegqnkklEEKDSKLAEANETSRALRDEIRELHHKIKNQEIQLTTQTTENQGLQEQLELLHKKCQEATQKI---- 207
1_pfamImport  80 slrknldglnrkvEERDTRLNTANQTISSLKDEIRELKQKIQNQDSAVSSHTLENQELQEHLDLQRRKYQEVSQLC---- 155
NP_957206    208 QNLQSSHSTSTESELKMKELERRLAL------QEQDSVIVKNMKSEV-ARLPEMEREIKRLREENSFLRETKENNSLLKE 280
P87169       193 EQLETKCKELAAAEQQLQELSVHNQQleesikQVSSSIELEKINAEQrLQISELEKLKAAQEERIEKLSSNNRNVEILKE 272 Schizosaccharom...
XP_013025869 180 EQLNSKLKESLDKEQQVQTLIFEIEKlkesvnQASDSLELKDANQKLlERIAELEKLQEKHLYMIERYNTNSRNIEIVNE 259
XP_002063744 239 SNYEKQQHSLKTANERIKELEYEIQS------YNDWKEVTKASQDRL-ASIPDMQAELDRLRGHNKHLNSLIGDKLLLEE 311
XP_002050330 241 MSYEMQQQTLATANARIKELEYEIQS------YADWKEITKTSQARL-LSVPELQSELERLRAHNKQLNSLIGDKLLLEE 313
Q7KND8       236 ANHEKQTQSLKVANDRIQELEFEIQS------YSDWKEVVKTSRERL-ASVPDLLAEVEHLRSHNKHLNTLIGDKLLLEE 308 fruit fly
XP_001959874 235 SDFEKQSQSLKAANERIKELEYEIQS------YNDWKEVTKASQERL-ASVPDLQSEIERLRHHNKHLNTLIGDKLLLEE 307
XP_002018198 238 ANFEKQQQSLKVADERIKELEFEIQS------YTDWKQVTKVSQERL-ASIPEMDSELQRLRVHNKQLNKIIGDKLLLEE 310
XP_005054501 209 QTLQASQSLLAEYEQKIKDLEQKLSQ------QEHDALIVKNMKAEL-ARFPKMERELRQLKEENAYFREMKENNGLLKE 281
Q9WTX8       209 QELQASQDERAEHEQKIKDLEQKLCL------QEQDAAVVKSMKSEL-MRMPRMERELKRLHEENTHLREMKETNGLLTE 281 house mouse
NP_001095514 209 QELQASQEVRADQEQRIKDLEQKLSL------QEQDAAIVKNMRSEL-VRLPTMERELRQLREENACLRETQDTNGLLRE 281
XP_006637049 208 QALQMYPSLNSENELKIKELERRLAM------QEQDSVIVRNMKSEL-GRIPDMEKELQQLREENAYLRETKENNNLLKE 280
1_pfamImport 156 QSLQAAQSSCSEHIITIKELERRLAL------QEQDFAIVKNIKSEV-ARVPDLERDLKRLREDNAFLRESRENCSLLKE 228
NP_957206    281 ETEGLRRKLERLEKVMEEKIKVELEKEKMERELQAWENIGQAtg--lNIRKPEDLSREVIQIQQRELNFKQQNYSLNSSV 358
P87169       273 EKNDLESKLYRFEEYRDKVATLELENEKIQTELNSWKSLITN-----ELPTPEAVSNKLVFLQNTNANLGERVSSLESQL 347 Schizosaccharom...
XP_013025869 260 EKADLEAKLYQYEDYKEKVAVLGVDNEKLQAELNSWKKTFM------DMNVPELAAEKFKTLQSENKTLSERVSDLTQQL 333
XP_002063744 312 QVHHYKTRLEKEEGARAEAASLQVKLSHVEQELKEWVKIAQDhclanTLVSPIALRARIEQLLQEDIVHVAEKTSSESDS 391
XP_002050330 314 QVHDYKTRLDREEGARAEAATLQVKLTHVEQELKEWVKVAQDhclpnTLVSPMALRTRIEQLLQDDIVRMAEKGSADSDS 393
Q7KND8       309 QVYDYKTRLEREEGARAEAASLQVKLLHMEQELKEWVKVAQDhclanTLVSPMALRSRIEQLLKEDIIHVAEKTSSASDT 388 fruit fly
XP_001959874 308 KVHDYKTRLDREEGARAEAVSLQVKLSHVEQELKEWVKVAQDhclpnTLVSPMALRSRIEQLLQEDIVHVAEKTTSESDT 387
XP_002018198 311 QVHEYKARLDKEEGSRAEAAALQVKLSHTERELKEWVKVAQDhclanTLVSPVTLRSRIEQLLQGDIVHVSEKYASESES 390
XP_005054501 282 EVEGLQRKLERYEKVQAQLVTVELENEKLLGKLQSWEKLDQStg--lNIRTPDDLSRQIVALQQRELALKEQNSTFMNSA 359
Q9WTX8       282 ELEGLQRKLSRQEKMQEALVDLELEKEKLLAKLQSWENLDQTmg--lNLRTPEDLSRFVVELQQRELTLKEKNNSITSSA 359 house mouse
NP_001095514 282 ELEGLQRRLGRQEKMQETLVDLELEKERLLTKLQSWERLDQTtg--lSIRTPEDLSRFIVELQQRELALKDKNSAITSSA 359
XP_006637049 281 EAEGMRKKLERVEKMKEDLVKLELEKEKLSLKLQAWQNLEQMtg--lNIRRPEDLSGEIIQFQQREITLKQQNYSVTSSA 358
1_pfamImport 229 EVEGLRKKLERTERTKEELVNMELEKERVAEKLRAWETIGQStg--lNIRKPEDLSREVIHIQQIEISLKEQNYTLNSRV 306
NP_957206    359 RSVEKARSELLTEIAQLraKAQEEQKKRENQDSLVRRLQKRVLLLTKERDGMRAILESYDSE---LATSEYSPQLTLRVK 435
P87169       348 SNKPANQPLGANEKDAA--HITELETKLKELHEQNRRLQRQKSLATQEIDLLRENLKSYDDEeaiLSEKNTDMKKLERIE 425 Schizosaccharom...
XP_013025869 334 LQERIQGPPMVIELKN---KVTDLKNENKETSEQIRRLQRQVFLANQEINLLRANLKSYDDEeaiMNNEKSGQKKLERIE 410
XP_002063744 392 KHLHSTIKDLEQKCAAYlkNIEDLNIGLKRHKNFKERLQRKLIIVSKERDFYKQLLENFDKD---QTLNNTS-------- 460
XP_002050330 394 KQLQSSLRELEQKCAVYlkNIEDMNVVLKRHKSFRERLQRKLLIVCKERDFYKQLLENFDKD---LNMTNAS-------- 462
Q7KND8       389 KHLNTTIRDLEHKCAIYlkNIEDLNIGLKRHKNFKERLQRKLITVSKERDFYKQLVENFDKD---TTLSNAS-------- 457 fruit fly
XP_001959874 388 KHLQATVRELEQKCSVYlkNIEDLNIGLKRHKNFKERLQRKLITVSKERDFYKQMVENFDKD---LTMSNTS-------- 456
XP_002018198 391 KQMRNLVRDLEQKIRVYlkNIEDLNMSLKRHKNFKDRLQRKLRTVSRERDFYKQMVDNFDKD---LTMSNAS-------- 459
XP_005054501 360 RMLEKARQQLQEEILSVqsQLLDEKKKREHQEALVRRLQKRVVLLTKERDGMRAILESYDSE---LSPAEHSPQLSRRMR 436
Q9WTX8       360 RGLEKVQQQLQDEVRQAnaQLLEERKKRETHEALARRLQKRNALLTKERDGMRAILGSYDSE---LTQTEYSTQLTQRLW 436 house mouse
NP_001095514 360 RELEKARLQLQDEVRQLggQLLEERKRRETHEALARRLQKRVLLLTKERDGMRAILGSYDSE---LTPAEHSPQLTRRMR 436
XP_006637049 359 RALEKVRLELQNELLQLraKLLEEQKRREQQDALVRRLQKRVLLLTKERDGVRAILESYDNE---LTASEYSPQLSQRVK 435
1_pfamImport 307 RSAERSQAELRAELSKQrgRTQEEQKKREAQDALVRRLQKRVLLLTKERDGMRAILESYDGE---LAPAEYSPQLSKRLR 383
NP_957206    436 EAEDMLQKVQAHNAEMETQLSKAQeeagsfklqaQMVAAELEALKeQQVSNAERSSsvtaeeISSLRQKIEELEAERQR- 514
P87169       426 GLVKLVDEYKLKLESMPVSLDVDE----------TSDEVSLQKRR-RKNEHKDAGY------VTELYRKNQHLLFQVKEk 488 Schizosaccharom...
XP_013025869 411 SLTQLIDEYKVLFENSKIynq-----------gaSADEQSLTLKRpRNEDQEDVGY------VTELYRENQHIQLQLQEk 473
XP_002063744 461 -AADMTQDMH-----VRHRIEVLErtvtgykdicATLEREIQAMR-QQEQLHEPtn----egYDSVKKELDTLRLENER- 528
XP_002050330 463 -TAEMTNDMQ-----VRYRVEVLErtltgykemcSTLEREMQSMR-QQESLNDPPise--ngYDSVKRELDTLRMENER- 532
Q7KND8       458 -VADMTQDMQ-----VRVRMEVLErtvtgykdmcATLEREIQSLR-QQELVNEPag----egYDSVKKELDTLRMENDR- 525 fruit fly
XP_001959874 457 -MADMTLDMQ-----VRYRMEVLErtvtgykdmcATLEREIQAMR-QQEQLAEPsg----egYDSVKKELDTLRLENDR- 524
XP_002018198 460 -VAEMTQDMQ-----VRYRVDVLErtltgykdlcTTLDREIQAMR-EQEQLSDPss----edYENVKKELDTLRLENDR- 527
XP_005054501 437 EAEDMVQKLHAHNAELEAQLSQVLeevgshkqraEMLEVEMKVLK-SQQCTAEQSTvvtkeeVDTLRLKIEELEAERSK- 514
Q9WTX8       437 EAEDMVQKVHAHSSEMEAQLSQALeelgvqkqraDTLEMELKMLK-AQTSSAESSFsfckeeVDALRLKVEELEGERSR- 514 house mouse
NP_001095514 437 EAEDMMQKVQAHSSEVEAQLSQALeelggqkqraDMLEMELKILK-SQAAPAEQSFlfsreeVSSLRLKVEELEAERSR- 514
XP_006637049 436 EAEEMLQKVQTHNSEMEAQLSKAQeeagayklqaQVAEAEIDILK-KQAATADNSSfvsteeVNSLRQKIEELEAERRR- 513
1_pfamImport 384 EAEEVLQRTQKHNVEMEGQLTKAQeeagtlklqlHAVELEMESLRkQQASAGDGSSsetkeeVSILRQKIEDLEAERQS- 462
NP_957206    515 ------LEEQNNILEMRLE--RHNLQGDYDPVKTKVVHLQMNPTSMAKQQRADEVEQLRVECQRLRDRLRkievag-gmt 585
P87169       489 tnieafLREQIITLESSIAtlRQELAQVTEINSCRVLQHRSNPTLKYERIKAAQLEMLNAENSALKALLE---------- 558 Schizosaccharom...
XP_013025869 474 lntegfLRDQINGLEVIIAelRQEIIQMSELKSSKVLQHRSNPTLKHERVKAEQLHLLEKENGGYKAILE---------- 543
XP_002063744 529 ------LRRRKDELELEMV--NRSLRGT-DTANHKVVHLSQNPAAEAYECSKNMMEKLQAEIERLKRRIKkme------e 593
XP_002050330 533 ------LRRRKDELELELE--HRCLRGDFNMGNYKVLHLSENPAADAYEATKNVVEKLQAEIERLKRRNKkle------e 598
Q7KND8       526 ------LRRRKEELEMEMM--HRCLRGDFNMKDFKVVHFSENPAAEAYESTKNMMEKLQAEIERLKRRNKkle------d 591 fruit fly
XP_001959874 525 ------LRRRKEEMELELM--HRCLRGDFNMKEFKVVHLSENPAAEAYESSKNVMEKLQAEIERLKRRNKkle------d 590
XP_002018198 528 ------LRRRKEEQELEIM--QRCLRQDISPPISKVVHLVDNPAAEAYESSKNMLEKLQAEIERLKRHNKkle------d 593
XP_005054501 515 ------LAEENRSLEMKLE--KLTLQGDYDPSRTKVLHLSMNPMSLAKQQRKEEQQQLQEECERLRELVRvlkgggslsg 586
Q9WTX8       515 ------LEQEKQVLEMQME--KLTLQGDYNQSRTKVLHMSLNPISMARQRQHEDHDRLQEECERLRGLVHalerggpipa 586 house mouse
NP_001095514 515 ------LEEEKKTLEAQLE--RLTLQGDYDQSKTKVLHLSQNPAGAARQRLREDQQQLQEECARLRELVRaleaggpvpa 586
XP_006637049 514 ------LEEQNNMLEMRLE--RHNLQGDYDPTKVKVLHFRMNPASQARQQRAQDAEQLRQELQRLKEHIQllqagg-ava 584
1_pfamImport 463 ------LEQQNNVLEMRLE--RHNLQGDYDPVKTRVLHLKMNPTATAKQQRQQDGEALREEVTRLREVVRsdesglslpp 534
NP_957206    586 tdD-TTLIIPPS--------------QEILDLRKQMESAELKNQRLKEVFQKKIQEFRTACYVLTGYQID-ITVENQYRL 649
P87169       559 --DkKVDCLPIQsfk--------iaeRKALDLKKEVAEREKRIQRLKEIFSVKSLEFREAVFSLFGYKLD-FMPNGSVRV 627 Schizosaccharom...
XP_013025869 544 --EkDIQTVPMEsle--------lanRRTLELKKELLDRDKRIQRLKEIFSVKSLEFREAIYSLFGYKIE-FLPNGSVRV 612
XP_002063744 594 dsEq-----ATTtditsasagmtmnfKELNQMRADLESANAKMRKMKDCFKAAGKEFRDVVYMLLGYRVDrMGANSNYRI 668
XP_002050330 599 dqEqTQARFNETvgsssagggmtmnfKEFNQLRAELESANGKMKKMKDCFKAASKEFRDVCYMLLGYRIDrVGANSHYRI 678
Q7KND8       592 dnEq---RLNETtstgg----mtlnfKEFNQLQAELESANGKMRKMRDCFKAAREEFRDVCYMLLGYRIDrIGANSNYRI 664 fruit fly
XP_001959874 591 dqEq---RLNETtstgg----mtmnfKEFNQLRAELESANGKMRKMKECFKAATTEFRDVCYMLLGYRIDrIGANSNYRI 663
XP_002018198 594 anEq---HLNETtststg--gmtmnfKELNKLRAELESANAKLGKTKEYFMAARKEFRDVVYMLLGYRIDrVGYKSNYRV 668
XP_005054501 587 elE-GVGAFQSP--------------QEVAELKKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQID-ITTENQYRL 650
Q9WTX8       587 dlE-AASSLPSS--------------KEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQID-VTTESQYRL 650 house mouse
NP_001095514 587 hlE-AGAGLPSS--------------REVAELKKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQVD-ITTESQYRL 650
XP_006637049 585 aeE-GPLSLPPS--------------QEVLDLRKQVESAELKNQRLKEIFQKKIQEFRKVCYMLTGYQID-ITTENQYRL 648
1_pfamImport 535 pkE-VLGSFLCN--------------HNLVNLRKQMESSELRNQRLKEVFQRKIQEFRTVCYVLTGYQID-ITTENQYRL 598
NP_957206    650 TSVYAEHMEDSLLFkstgPVGsGSMQLLETDFSRTLTGLVDLHLFHQKSIPVFLSAVTIELFSRQT 715
P87169       628 TSTYSREDNTAFIF----DGEsSTMKLVGNPSGPEFERLIRFWCDERKTIPGMLAALTLELLDKND 689 Schizosaccharomyces pombe 972h-
XP_013025869 613 TSMYSHENNTAFVF----DGEtSTMKLVGKPSSPDFERLVRFWCDERKTIPGMLAALTLELIDKND 674
XP_002063744 669 SSMYAENPTDYFDItlseSNC---LALLESPYSQTLKPAIDQQLAANNSFPAFFSSLTLELFQRST 731
XP_002050330 679 SSMYAESPDDYLSIslneSNC---LALLESPYSQTLKPAIDQQLAANNSFPAFFAGLTLELFQRAT 741
Q7KND8       665 SSMFAEGPDDYLDIslneSNC---LALLESPYSHTFNPPIDQQLAASN-FPAFFSALTLELFQKAT 726 fruit fly
XP_001959874 664 SSMYAESPEDYLDItlneSNC---LALLESPYSRTLKPAIDQQLAASNSFPAFFSALTLELFQRAT 726
XP_002018198 669 TSMYAESPDDYFSIalseSND---LALLETPYSETLQPALDQQLAANNSFPPFFSSLTLDLFQRAT 731
XP_005054501 651 SSIYAEHQGDCLLFkassSSG-GKMQLLETEFSRTVRELIELHLLRQDSIPAFLSALTLDLFSRQT 715
Q9WTX8       651 TSRYAEHQTDCLIFkatgPSG-SKMQLLETEFSRSVPELIELHLLQQDSIPAFLSALTIELFSRQT 715 house mouse
NP_001095514 651 TSMYAEQKADCLIFkaagPSG-TKMQLLETAFSRTVPGLIELHLLQQDSIPAFLSALTLDLFSRQT 715
XP_006637049 649 TSVYTERVEDSLLFkasgPTG-GNMQLLETDFSRTLHDLVELHLFHQKSIPAFLSAVTLELFSRQT 713
1_pfamImport 599 TSLYAEHMDDSLLFkkmgSNG-AKMQLMETEFSKTLGEMVALHLHHQKSLPAFLSAVTLDLFSRQT 663
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