3BXD


Conserved Protein Domain Family
MIOX
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pfam05153: MIOX (This model is not part of the current CDD release)
Myo-inositol oxygenase
MIOX is the enzyme myo-inositol oxygenase. It catalyzes the first committed step in the glucuronate-xylulose pathway, It is a di-iron oxygenase with a key role in inositol metabolism. The structure reveals a monomeric, single-domain protein with a mostly helical fold that is distantly related to the diverse HD domain superfamily. The structural core is of five alpha-helices that contribute six ligands, four His and two Asp, to the di-iron centre where the two iron atoms are bridged by a putative hydroxide ion and one of the Asp ligands. The substrate is myo-inositol is bound in a terminal substrate-binding mode to a di-iron cluster. Within the structure are two additional proteinous lids that cover and shield the enzyme's active site.
Links
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Statistics
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PSSM-Id: 516658
Aligned: 15 rows
Threshold Bit Score: 439.477
Created: 14-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
3BXD_A        41 LDRVFTTYKLMHTHQTVDFVSRKRIQYG--SFSYKKMTIMEAVGMLDDLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKD 118 house mouse
Q5REY9        37 LDRVFTTYKLMHTHQTVDFVRSKHAQFG--GFSYKKMTVMEAVDLLDGLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKD 114 Sumatran orangutan
Q9VTU9        34 KERVRQTYRQMHLNQTVDFVKGRREHWL--KFNTIKMTVREALEKLNDLVDESDPDLDLPNIIHAFQAAERARAEFPEHD 111 fruit fly
NP_001408551  58 KETVEEFYRVNHINQTYDFVRRMREEYG--RVDKTEMGIWECIELLNEFIDDSDPDLDMPQIEHLLQTAEAIRKDFPDED 135
O82200        67 QQGVEEFYRMQHIHQTYDFVKKMRKEYG--KLNKMEMSIWECCELLNNVVDESDPDLDEPQIQHLLQTAEAIRRDYPDED 144 thale cress
Q9FJU4        64 QKSVEHFYATQHTNQTLDFVQKMRSEYG--KLDKMVMNIWECCELSKEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNED 141 thale cress
Q6CNQ4        71 KDRVREFYKEQHEKQTMAYNLQARINFK--TKVRARMTVWDALCQLSKLIDESDPDTELSQIDHALQTAEAIRND-GKPR 147 Kluyveromyces l...
XP_001275322  67 CDRVKNFYKEQHTKQTVAYNLKARNDFH--SKTRAEMTVWEAIEKLNTLIDESDPDTSLSQIEHLLQSAEAIRRD-GKPR 143
Q2GML9        63 CDRVKHFYAEQHAKQTVAHNLAARAHFHspSRKRPELGIWEAMEKLNTLVDDSDPDTSLSQIQHLLQSAEAIRRD-GKPR 141 Chaetomium glob...
XP_960986    102 ESRVLNFYTEQHTKQTVSHNLAARAHFNspDRKRPEMTIWQAIECLNSLIDESDPDTELSQIQHLLQSAEAIRRD-GKPR 180

3BXD_A       119 WFHLVGLLHDLGKIMAL--W---GEPQWAVVGDTFPVGCRPQASVVFcDSTFQDNPDLQDPRYSTELGMYQPHCGLENVL 193 house mouse
Q5REY9       115 WFHLVGLLHDLGKVLAL--F---GEPQWAVVGDTFPVGCRPQASVVFcDSTFQDNPDLQDPRYSTELGMYQPHCGLDRVL 189 Sumatran orangutan
Q9VTU9       112 WLHLTALIHDLGKIMAF--Y---GEPQWAVVGDTFAVGCRWGDSIVYrDESFEGNPDGDNPAYNTELGIYQPNCGVDNLL 186 fruit fly
NP_001408551 136 WLHLTGLIHDLGKVLLHpsFg--ELPQWSVVGDTFPVGCAFDECNVH-FKYFKENPDYLNPKLNTKFGAYSEGCGLDNVL 212
O82200       145 WLHLTALIHDLGKVLLLpeFg--GLPQWAVVGDTFPVGCTFDSANIH-HKYFKGNHDINNPKYNTKNGVYTEGCGLDNVL 221 thale cress
Q9FJU4       142 WLHLTALIHDLGKVLTLpqFg--GLPQWAVVGDTFPVGCAFDESNVH-HKYFMENPDFNNPKYNTKAGIYSEGCGLENVL 218 thale cress
Q6CNQ4       148 WMQLVGLIHDLGKILYF--Fe--SEGQWDVVGDTFPIGCGFVKENIF-PEFFQGNPDNSHPIYSTKLGIYEENSGLKSVM 222 Kluyveromyces l...
XP_001275322 144 WMQLTGLIHDLGKLLYF--Fd--AKGQWDVVGDTFPVGCAFDDRIIYgKESFKENPDYDNEIYRTEFGIYNPGCGLDNMM 219
Q2GML9       142 WMQLTGLIHDLGKLMLF--FegcCDGQWDVVGDTFPVGCRFDERCIY-PELFAANPDAGHEVYSTRYGIYQPGCGVNELM 218 Chaetomium glob...
XP_960986    181 WMQLVGLIHDLGKLMLF--Fe-lASGQWDVVGDSFPVGCKFSEKCIL-HESFSANPDSGHAVYSTEHGIYAPGCGIENLM 256

3BXD_A       194 MSWGHDEYLYQMMKFNKFSLPSEAFYMIRFHSFYPWHTGGDYRQLCSQQDLDMLPWVQEFNKFDLYTKCPDLPDVESLRP 273 house mouse
Q5REY9       190 MSWGHDEYMYQVMKFNKFSLPPEAFYMIRFHSFYPWHTGSDYQQLCSQQDLAMLPWVQEFNKFDLYTKCPDLPDVDKLRP 269 Sumatran orangutan
Q9VTU9       187 MSWGHDEYMYSVLKHNKTKLPHVACNIIRFHSFYPWHNGGDYKHLEAPQDAETKKWVLIFNRYDLYTKSEVVPDIEALWP 266 fruit fly
NP_001408551 213 MSWGHDDYMYLVAKENKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNERIDVEKVKP 292
O82200       222 MSWGHDDYMYLVAKKNGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQVKP 301 thale cress
Q9FJU4       219 MSWGHDDYMYLVAKENGSTLPSPGLFIIRYHSFYPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEKVKP 298 thale cress
Q6CNQ4       223 ISWGHDEYMYYIAK-NQSTLNEKALAMIRYHSFYPWHREGAYRYLMSESDYEILEAVQDFNQYDLYSKGLQKHNRDELKP 301 Kluyveromyces l...
XP_001275322 220 LSWGHDEYLYHVVK-EQSTLPDEALAMVRYHSFYPWHSAGAYHEFMNEKDHEMLKAVKAFNPYDLYSKSDDVPSIEELKP 298
Q2GML9       219 MSWGHDEYLYLVLR-DQSTLPREALAMVRFHSFYPWHKEGAYTEFMADGDEALLSAVRAFNPYDLYSKSDDVPKVDELKP 297 Chaetomium glob...
XP_960986    257 MSWGHDEYLYQVVK-DQSTIPREGLAMIRFHSFYPWHREEAYGWAMKEGDEELRRAVRAFNPYDLYSKSDEPVDVEAVKP 335

3BXD_A       274 YYQGLIDKYCPG-TLSW 289 house mouse
Q5REY9       270 YYQGLIDKYCPG-ILSW 285 Sumatran orangutan
Q9VTU9       267 YYQTLIDKYLPG-VLEF 282 fruit fly
NP_001408551 293 YYMSLIEKYFPA-KLRW 308
O82200       302 YYISLINKYFPA-KLKW 317 thale cress
Q9FJU4       299 YYMSLIKKYFPE-NLRW 314 thale cress
Q6CNQ4       302 YYLELIDEFFPQkLVEF 318 Kluyveromyces lactis
XP_001275322 299 YYLELIDEYFPNqVIKW 315
Q2GML9       298 YYLELIDEFFPQkVIKW 314 Chaetomium globosum CBS 148.51
XP_960986    336 YYMELIDEFFPNkVIKW 352
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