Conserved Protein Domain Family
Phage_holin_4_1

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pfam05105: Phage_holin_4_1 
Bacteriophage holin family
Phage holins and lytic enzymes are both necessary for bacterial lysis and virus dissemination. This family also includes TcdE/UtxA involved in toxin secretion in Clostridium difficile. The 1.E.10 family is represented by Bacillus subtilis phi29 holin; 1.E.16 represents the Cph1 holin; and the 1.E.19 family is represented by the Clostridium difficile TcdE holin. Toxigenic strains of C. difficile produce two large toxins (TcdA and TcdB) encoded within a pathogenicity locus. tcdE, encoded between tcdA and tcdB, encodes a 166 aa protein which causes death to E. coli when expressed, and the structure of TcdE resembles holins. TcdE acts on the bacterial membrane. Since TcdA and TcdB lack signal peptides, they may be released via TcdE either prior to or subsequent to cell lysis.
Statistics
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PSSM-Id: 398668
Aligned: 131 rows
Threshold Bit Score: 51.72
Threshold Setting Gi: 319417263
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
CCY23308            19 FMGKMgeiPQSISILLLMMVIDQVSGILAAKRealEHPgekkygISSKRMVLGIFKKAGYILTIVVATMVDILMDTmmey 98  Firmicute...
CCX89694             9 atAAS---NRFIILVLLAIVGDTLFGCFRAIK---EHK------FNSNFGIDGAIRKASMVAAILFFVFVDYIVQVnlig 76  Clostridi...
wucgs:EUBREC_1309   10 MTTAS---NKIMEIVVICIVMDTIFGVLRAIK---EKK------FNSNFGINGAIRKVGMLISLVLLALVDSIIRLnlig 77  [Eubacter...
WP_003023294        10 eAAAH---NKIIQLVVLAIVCDTVFGVLRAIK---EKK------FNSCAGIDGAIRKVGMLISLVFMLAIDVLIKInlig 77  Lachnospi...
jgi:Closa_1414       9 ALAWG---SPIIKLVILAVVMDTCFGCIRAIK---EHK------FNSCFGIDGAIRKISMVASLAFLLILDKIVHLnlig 76  [Clostrid...
CBL26417             6 tLLAS---NSFVKILLIAVALDTILGVLRAIK---EHK------FNSCVGIDGAIRKAAMLFSVCLLMAMDVIVHInvlc 73  [Ruminoco...
Q189K6              30 SFLSE---HIFIKLVILTISFDTLLGCLSAIK---SRK------FNSSFGIDGGIRKVAMIACIFFLSVVDILTKFnflf 97  Clostridi...
gigasnome:DGI_2379  29 LVGPV---DAVVWGLVTLLVLDFALGFGRAWT---AGT------LSGRRMLHGAGKFVLFGLAVVMGHVLDAMFLAgigl 96  Desulfovi...
Q72FL1              36 MLGGV---ANVLPFLAILLVLDYVLGFVRACR---IRR------ISGAKMRAGAWKFLFYFAAVFVVATVDGALGKawtl 103 Desulfovi...
Q72A31              15 LLGGV---GMMISLLFLLLVIDFALGFCRAWR---AGR------VSSSKLRGGGLKFVFYFLSIVVMAAVQAATSQstgm 82  Desulfovi...
CCY23308            99 igikstikgS-------FCLLITVWFILGEAISILENASRMGVKVP---RFFRKIISELQK 149 Firmicutes bacterium CAG:24
CCX89694            77 fi-pqeirdYlnlqsvgMTEFFALLFIAYEAVSIIKNMTLCGLPVKkvwIYLKNFLNKYTD 136 Clostridium sp. CAG:590
wucgs:EUBREC_1309   78 fi-pegartYlpgqtvgTMEFFALLYIAYEVVSILKNMSLCGLPVKkvwHTVKKALSKYTN 137 [Eubacterium] rectale ATCC 3...
WP_003023294        78 fi-peqartYlgldtvgVAEFFALLYIAYEVVSIFKNMALCGLPVKkvwEKVREFLAKYTD 137 Lachnospiraceae
jgi:Closa_1414      77 fi-peavraYlpvsgigVAEFFGLLYIAYELVSILKNMTLCGLPVKhlwEVIKKFLSQYTD 136 [Clostridium] saccharolyticu...
CBL26417            74 mi-peqyiqVlgisklgVCEFFSLLFILYELVSILKNMTLCGLPVP---TKIKIWIQKFLD 130 [Ruminococcus] torques L2-14
Q189K6              98 ml-pqdcinFlrlkhlgISEFFSILFILYESVSILKNMCLCGLPVP---KRLKEKIAILLD 154 Clostridioides difficile 630
gigasnome:DGI_2379  97 e------------fpvtCRNLIIVYLALSESLSICEHLACLNVPLP---ARLVARLKCYRD 142 Desulfovibrio gigas DSM 1382...
Q72FL1             104 -------------fhvpMGAFFVFYLCVNEAMSCLDHLKFFGVPIP---EWLSTRLRDYRE 148 Desulfovibrio vulgaris str. ...
Q72A31              83 --------------tipLRDVFVAYLCVNEGLSCLEHLSYFGVPVP---EKIRERLRAYRD 126 Desulfovibrio vulgaris str. ...
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