Conserved Protein Domain Family
Proteasom_Rpn13

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pfam04683: Proteasom_Rpn13 
Proteasome complex subunit Rpn13 ubiquitin receptor
This family was thought originally to be involved in cell-adhesion, but the members are now known to be proteasome subunit Rpn13, a novel ubiquitin receptor. The 26S proteasome is a huge macromolecular protein-degradation machine consisting of a proteolytically active 20S core, in the form of four disc-like proteins, and one or two 19S regulatory particles. The regulatory particle(s) sit on the top and or bottom of the core, de-ubiquitinate the substrate peptides, unfold them and guide them into the narrow channel through the centre of the core. Rpn13 and its homologs dock onto the regulatory particle through the N-terminal region which binds Rpn2. The C-terminal part of the domain binds de-ubiquitinating enzyme Uch37/UCHL5 and enhances its isopeptidase activity. Rpn13 binds ubiquitin via a conserved amino-terminal region called the pleckstrin-like receptor for ubiquitin, termed Pru, domain. The domain forms two contiguous anti-parallel beta-sheets with a configuration similar to the pleckstrin-homology domain (PHD) fold. Rpn13's ability to bind ubiquitin and the proteasome subunit Rpn2/S1 simultaneously supports evidence of its role as a ubiquitin receptor. Finally, when complexed to di-ubiquitin, via the Pru, and Uch37 via the C-terminal part, it frees up the distal ubiquitin for de-ubiquitination by the Uch37.
Statistics
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PSSM-Id: 398384
Aligned: 131 rows
Threshold Bit Score: 50.6385
Threshold Setting Gi: 122078868
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_003672395         14 GVAKYDe-------dTKICTPLPVQGQISIkkaggdgeDeEDEEdemsLYELIWKPMEK-VvgDNveycqELNLMLIPDD 85  Naumovoz...
Q22XT3               21 GIMNLDp-------tTKKVTADKRKGTVKIg------cTlEGEKp------FTWTPEGa---tEP-----EFEYAVMQSD 73  Tetrahym...
A0CU81                3 qevtipcgrydfnleTKKVVIVKAKGLLNL--------YlNDEN----ELWLKWYNVDLd--sKL-----EIERVLIKGS 63  Parameci...
EGD73164             38 GRMKIE---------GNRVTPLEAKGVLYL--------AkDDTN----LPVLCWKNRTt---gVV-----EDEIIVLPGN 88  Salpingo...
XP_001742579         76 GRMTQE---------GNTVTAVPGKGLVFL--------KrDGDL-------LMWHYKSRdSgrEE-----EEPIVLFPGE 126 Monosiga...
CAP33651             29 GRCKLSpidgsadpnIRKVIAQPEKGVLYL--------IlDRDN----VPRLRWASRLt---gTI-----KKDDFVFPHE 88  Caenorha...
EGT60157             20 grslvlpvegsndpnRKKVVAQPEKGLLYV--------DlDDMN----IPHLRWKNRTt---nEI-----EMDLMILPDE 79  Caenorha...
XP_002057009         81 GRMNVGe-------kM--VEPDQRRGLLYL--------HrDA-Eq---HLHFCWKDRKs---dTV-----EVDIMSEPGY 131 Drosophi...
WGS:AADE:GA22734-PA  10 GLMVLRa-------gM--FEPDSRKGLLYV--------ClM--Et---EIHICWKDRRt---dQV-----ELDILAVPGV 59  Drosophi...
WGS:AAPP:GF21327-PA  68 grMVLQ---------GKMVQPDDRKGLLFV--------R-RSAGn---QVHIHWMDRHs---gDI-----ELDVVASAGS 118 Drosophi...
XP_003672395         86 TQWVSVKSC-KNGRVFALVY--SSNQK-----YFFWLQE 116 Naumovozyma dairenensis CBS 421
Q22XT3               74 ASLELs---kQMKRVVVLRFq-CDESR-----YFFWLQE 103 Tetrahymena thermophila SB210
A0CU81               64 TFFEKVK---GQNRVYLLRFn-DDDQK-----YFFWMQS 93  Paramecium tetraurelia
EGD73164             89 QKFYKCKSApEGSRVYYLKM---SDER-----HFFWMQE 119 Salpingoeca rosetta
XP_001742579        127 QTFQLCKSApASSRVYYLKF--SGGRR-----DFFWMQS 158 Monosiga brevicollis MX1
CAP33651             89 VTFKKVKN--DDGKTFIVKNldMGIKSc----LAFWLQN 121 Caenorhabditis briggsae
EGT60157             80 VVFKKAQEN-QDGKVYIVKYveARVATqeggvFAFWLQD 117 Caenorhabditis brenneri
XP_002057009        132 LEFRRVEPC-KTGRVYVLKFr-RSIKR-----LFFWMQD 163 Drosophila virilis
WGS:AADE:GA22734-PA  60 PRFQRVSQV-NTGRVYVLRFe-GARER-----HFFWMQE 91  Drosophila pseudoobscura pseudoobscura
WGS:AAPP:GF21327-PA 119 LEFRRVEEC-KTGRVYVLKFr-GSVRR-----YFFWMQE 150 Drosophila ananassae
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