pfam04167: DUF402 (This model is not part of the current CDD release)
Protein of unknown function (DUF402)
Family member FomD is a protein encoded in the fosfomycin biosynthesis gene cluster, which hydrolyzes (S)-HPP-CMP to give (S)-HPP and CMP in the presence of Mn2 or Co2. FomD also hydrolyzes cytidylyl 2-hydroxyethylphosphonate (HEP-CMP), which is a biosynthetic intermediate before C-methylation. FomD structure revealed that it has a beta-barrel fold consisting of a large twisted antiparallel beta-sheet, a key feature of DUF402-containing proteins. The function of this domain is unknown. It has a Tyr residue which activates a water molecule to promote nucleophilic attack on the phosphorus atom of the phosphonate moiety. This domain has also been found in Ntdp (nucleoside tri- and diphosphatase, also known as Sa1684) from Staphylococcus aureus.
Jiyao W et al.(2023). "The conserved domain database in 2023.",