8J4C_B                  18 RGRIAFNSAFRDVLLFKDNYLFKLAVFTLALEMILFVLLS--QVGLM-----QM-------NPKPLNl--VG-N--IIGG 78  synth...
Q0KAQ6                  26 RGATCAVAAVSEITSSRSCSHLRAMAEASLYVCGGLLIAQ--RLHLL-----PA-------MPSGYPv-dGL-T--VLGG 87  Cupri...
WP_012959188            66 HARFGFTSAFRRLMSVGNGEGLRAHMLMLAVASSLFAPIL--AYGAS-----FYgh----dPTGFVSp--VG-TslLVGA 131 Alkal...
WP_013673810            78 HSRFGFTSGWRQLAAVGQGAAVRAHMLMLAVACVLFSILLagGFSLA-----GT-------PKPALAg--VG-VplLVGA 142 Pseud...
WP_012487944            53 RSKFCFFCITRDFVAQRNSLGLLGLVAALATGILGYSAVY--GAILPtpvegRLp------PDAHIGp--VSwVl-VFAA 121 Cellv...
PRJNA63271:OPIT5_26465  55 RSRFCFYCGLREFIEERDARPVLGLLAALAAGTVGYLVIF--GA-WV-----PFpeaghlpPRAHIGpagWH-L--LVGG 123
XP_002680827            28 KSRMSAPENIKNQMYLKDWTMMKTFLSASGTSTISLGLLN--LFNLA-----TL-------APFNINw--RG-N--IIGG 88 
XP_002679831            26 KSRMSSPENIKNQMALKSWSMMKTFLSASGASTISLGLLD--LMGVA-----TL-------SPININl--RS-T--ISGG 86 
XP_004367137            37 RSKVFLPIVIQQQMQFTNFTMLKMFMTASLTSSLTITALN--KAKLI-----KF-------EYLPVMw--HR-N--LLGG 97 
XP_003284822            39 RGNVYLPSVIQGQMNFSNFTMLKMFMTASLTSSLAVTFLN--HRKLI-----TI-------ESLPVMy--KR-N--LIGG 99 

8J4C_B                  79 FVFGLGMVLAGGCASGVTYRVGEGLTTAWFAALFYGLGAYatksGAFSWWLSWVG-QF--KSplsveesayyvkgagpTI 155 synth...
Q0KAQ6                  88 ALLGLGAFINGACVFGAIARLGSGQWAYAATPLGYYAGCV----GLLALWPQAER-PA--LS----------------AT 144 Cupri...
WP_012959188           132 FIFGIGMQLGGGCASGTLYSVGGGRSSMFITLFGFIAGSVig-aAHFDFWMNETP-TL--PAis-------------lAT 194 Alkal...
WP_013673810           143 FLFGAGMQVGGSCASGTLFAVGSGQTSVVLTLFGFVVGSVla-aATFTFWTVTIP-AG--Ps---------------iSL 203 Pseud...
WP_012487944           122 LVFGSGMSLSGSCISAHLYRLGEGALASAVALIGVILGFIlgfaTWNDLYLRFIQ-EA--PVvw-------------lPH 185 Cellv...
PRJNA63271:OPIT5_26465 124 FAFGAGMALSGSCLSAHLYRLGEGSLLAPVALAGAAGGFIlgfnSWEFFYLHAVT-EA--PVv---------------WL 185
XP_002680827            89 LIMGTGLTLGGACPGTVLAQIGEGVTSSPYTLLGGLLGAIsygsIINIPSIQGIL-QS--KEvsg----------sfnTI 155
XP_002679831            87 AIMGVGMALGGACPGTVLAQAGEGVPSSLYTLLGGIIGATvygiVINNPTVNSLL-ES--TEvp-------------gSF 150
XP_004367137            98 LLMGAGIYVTGACPGTVLAQVGSGVTSAYYTLLGGLLGav--lyGYADPYVTKLM-PAspPTvk-------------pTL 161
XP_003284822           100 LIMGSGIYLTGACP-GTVLAQVPEIKGTLFTLLGGVV--GatfyGYIDKIINKLFpAK--NDdk-------------pTL 161

8J4C_B                 156 SSVLGL--N---PWIPA-----LVIAALF--ILW-----------------AFGTKT-TSRETKFNWKIASVCLALV--A 203 synth...
Q0KAQ6                 145 SPVWGA--A---PLLTVl---fLLFVGWR--LLRlwqrarr---hgapaplEQTWHA-RVAARIWSPHHATTVIGVT--F 208 Cupri...
WP_012959188           195 DTGLGY--G---GAWLLq---lVIFAGII--FLTyyiqrkkn-ppkmalvpSAQGMK-RIFRGSWPLLIAGVVLAVL--N 260 Alkal...
WP_013673810           204 ARTFGY--PgalALSLAv---mAVVVAVT--YVVawrrtpp---pvqrpasARGI-A-RVLRGSWPFWVGAVALAVLnaG 271 Pseud...
WP_012487944           186 YWGYGG--A---LLIQLg---lLGFI-AW--LLVkhhrtv------dtqhtpVSFFT-AWFVNRWPTYLGGMLIGSL--A 245 Cellv...
PRJNA63271:OPIT5_26465 186 PRTAGG--Y---ALAAVv---qLAVIGLLafLVWrrgrvdplpsaarpprtLATLAE-AVFVRRWPHWIGGVGAGLL--G 254
XP_002680827           156 NG-LLN--Q---PMWSIalpfgSCLIGIA--LLLeklfptpk-yqssnnknDRN----LMKMKQWNPILSGIALGSL--Q 220
XP_002679831           151 NTLHGLlgK---PMWSIa---lPVGTMLV--GIActleklf----ptpetdLISTEKcLLKRRQWHPIYSGLALGAL--Q 216
XP_004367137           162 FQRLNT--S---MEKTTi---pFALMAGA--MLYgieslipw-qadsnvslSISAIQ-SLSTSVWSPYVAGVLIGLL--Q 227
XP_003284822           162 YQKLNV--S---MAKVTi---pFALMLAG--VLYtiesffpw-qsdsgvspNTSI---LGSDSVWSPYRAGLTIGLL--Q 225

8J4C_B                 204 GL-----GFI-------------TSTL-Sgrkyg----lgitgGWINLFQGFLT------------------N------- 235 synth...
Q0KAQ6                 209 LL-----LLL-------------LAGA-W--------------SYTEVLAELARg----------------sA------- 232 Cupri...
WP_012959188           261 AV-----TLL-------------VRgqpwgi------tsafalWGSKAAQFIGIdvaswgywsgeraqelnqS------- 309 Alkal...
WP_013673810           272 VLfvsggAWG-------------VTSA-Fglwggkv-lsglgvDVASWAYWQVPa----------------nS------- 313 Pseud...
WP_012487944           246 TV-----AYF-------------RIAP-LgvtaelgslartlgSEANILPSRLEg----------------lD------- 283 Cellv...
PRJNA63271:OPIT5_26465 255 AA-----VYLratplgvtaelgrLSRK-Lgthwgv---splslPGLDGLAGCATgdp-------------agA------- 305
XP_002680827           221 IP-----VNL-------------FV-K-Tylgts----safvtAASFLTRYLPVn----------------qS------- 253
XP_002679831           217 VP-----VNL-------------ICKT-Tlgtss-----afvtAVTFITRYLPVe----------------qEyferfti 256
XP_004367137           228 AP-----SYF-------------LAN--Nglgcs----sayvtMSAKVCDAFNCgvs-------------yfN------- 263
XP_003284822           226 IP-----THL-------------ISKGgLgcssay---vtigsKVCNAFSCTVDy----------------fK------- 261

8J4C_B                 236 ----SP-----------LN------WEGL------EIVGIILGAGVAAAVAGE-FKL--Rm-PKnPVTYLQVGIGGLLMG 284 synth...
Q0KAQ6                 233 ----AM-----------LP------ARAL------LSLGLLAGAILGGMSAGR-REG--Pa-TS-SAQCLRCFTGGAIMG 280 Cupri...
WP_012959188           310 ----VF-----------LD------STSV------MNFGIMAGAFIAAAAGGV-FVL--Kk-IP-PKVMIASLIGGIMMG 357 Alkal...
WP_013673810           314 ----KA-----------LH------NSVFadrisvLDFGVMIGALVASALGGA-FVL--HrrVP-WRPALGSVLGGVAMG 368 Pseud...
WP_012487944           284 ----SFsgcataiketlLS------TNGV------FIVGLVFASFAASLFSGQ-FAF--Dl-PD-LRKVLRHFVGGILMG 342 Cellv...
PRJNA63271:OPIT5_26465 306 ----AA-----------LT------PNAL------FVVGLVAAAVAAGVGSGA-FVP--Er-PP-WPRVVLALAGGVLLG 353
XP_002680827           254 ----YFdk-------yaLSagpkyvWQLI------SDISIITGSYISSIM-GN-TRVqpKk-LT-LNEKLMAIASGFLLL 312
XP_002679831           257 sksaKY-----------L-------WQLI------SDLSIVGGSYFSSYLAGTrAEP--Kk-LS-NKEKCWAIASGFLLL 308
XP_004367137           264 ----KF-----------ANgvr-svYGPM------LNIGIILGAYLSSQTSPV-PDS--Qk-LL-SHSPLFHIGAGALLL 316
XP_003284822           262 ----KFnsg------skAY------FGPL------LNAGIIFGAYLSSLSNPA-PPL--Da-AGgSQTYLTHFLGGFILL 315

8J4C_B                 285 IGAVTAGGCNIGHFLTGVPQLALSSWLASIFFIL-GN 320 synthetic construct
Q0KAQ6                 281 WGGMLIPGGNDGLLLVGMPLLWPYAWVAFGTMCV-TI 316 Cupriavidus necator H16
WP_012959188           358 YGARLAFGCNIGAYFSGVASFSLHGWVWAVMALA-GT 393 Alkalihalophilus
WP_013673810           369 YGARLAGGCNIGAYFSGIASFSLHGWIWGAAALL-GT 404 Pseudonocardia dioxanivorans
WP_012487944           343 WASMTALGCTVGTLLSGIMAGALSGWIFAVFCII-GL 378 Cellvibrio japonicus
PRJNA63271:OPIT5_26465 354 FGSMISLGCTIGTLLSGVNALALSGWIFAAALFG-GV 389
XP_002680827           313 FGARCANGCTSGHGLSQMANLSFAGLLTIASMMAsGI 349
XP_002679831           309 FGARAGHGCTSGQGLSQMANLSFGGMIGVASMLAsGF 345
XP_004367137           317 FGARMANGCTSGHGLTGMAKMELGSIITVAALFG-GG 352
XP_003284822           316 IGARIANGCTSGHGLNSMAKMELGSFIAVASLFG-GG 351