Conserved Protein Domain Family
GSP_synth

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pfam03738: GSP_synth 
Glutathionylspermidine synthase preATP-grasp
This region contains the Glutathionylspermidine synthase enzymatic activity EC:6.3.1.8. This is the C-terminal region in bi-enzymes. Glutathionylspermidine (GSP) synthetases of Trypanosomatidae and Escherichia coli couple hydrolysis of ATP (to ADP and Pi) with formation of an amide bond between spermidine and the glycine carboxylate of glutathione (gamma-Glu-Cys-Gly). In the pathogenic trypanosomatids, this reaction is the penultimate step in the biosynthesis of the antioxidant metabolite, trypanothione (N1,N8-bis-(glutathionyl)spermidine), and is a target for drug design. This region, the pre-ATP grasp region, probably carries the substrate-binding site.
Statistics
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PSSM-Id: 397691
Aligned: 65 rows
Threshold Bit Score: 306.403
Threshold Setting Gi: 506344255
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
WP_015863974  22 WADLYGAE------------YALFDYYTLTAREVEQIRTTTDRIGHLYRKTAKLLRQLPdETLRlLGFHPDTLPFLRAst 89  unclassified Ge...
WP_015843788  19 WADLYGEE------------YSLYDLYDMTKEEVHKIRESTNRIGHIFYKTAQLLRTVDdETLLmLGFPRETLAYIRIrs 86  Paenibacillus s...
Q7MSC7        10 SNEFLESIgfawhtdpnqtsYVANEAVVLSEAEAEGFYSAANELYDMFVEAGQYVIDNN-LFFE-LGIPFNLIESIKM-- 85  Wolinella succi...
Q7VK77        10 DKNVLESMgltwhtdmdntsYLSDELIHISGAEAEAYYEAGNELYDMFIEAGGYVIKND-LFFE-LDIPPSLIGAIKQ-- 85  Helicobacter he...
CBG39869      10 DEKTLEQIglqwhsdedqtpYISDELIVVSEEEAEAFYEAGNTLYDMFVEAAEYVIEKE-LFFE-LDIPNSIIPMIKQ-- 85  Helicobacter mu...
O25019        10 dnKTLEEIgldwhtnddmssYIADEMVVVSQKEADAYYDACNELYDMFVETAEEAIQND-RFFE-LDIPNALIPMIKQ-- 85  Helicobacter py...
Q30QI3        11 KPAQLEELgftwhtdsdesnYISDTLVNITHDEAEAYYEAANEIYDMYAKAAEYVIENN-LFFE-LGIPFNLIEAIKK-- 86  Sulfurimonas de...
WP_012855894  11 SKDFLESIgfywhtdsdetsYVADELVLVSHDEVEAYYEAANELYDMFAEAGQYVIDNN-LFHE-LNIPFNLVELIKN-- 86  Sulfurospirillu...
WP_004509254  10 TDEYLESIgfiwhtdkdnssYISDEIVVISEDEANAFYEATNELYDMFVEAGQYVIDND-LFHE-LNIPFNLVEIIKE-- 85  Arcobacter butz...
BAF71572      11 TTNYLESLgfvwhtdsdesaYISNELVVISEDEAEAYYEAANTLYDMYVETGEYIVENN-LFHE-IGIPFNLVEVIKE-- 86  Sulfurovum sp. ...
WP_015863974  90 lpaeTV--------IARADLVYVd---gTFKLIELNADTPTFIRETFDINEQVASFfrmaspNEGAAKNVTkAVRHAVWQ 158 unclassified Ge...
WP_015843788  87 lsveSI--------IARLDLVGAg---eTYKCMEINSDTPTFIKELHHVNGLVCEAfdrhnpNKGYERQLAlAIEKAVVE 155 Paenibacillus s...
Q7MSC7        86 ----SWenevhwhiYGRFDFAggl-egrPIKLLEFNADTPTMVYETAIIQWALLKAn-----GMENHLQFN-NLYQAIGE 154 Wolinella succi...
Q7VK77        86 ----SWeedihwhlYGRFDLAggl-dgtPIKLLEFNADTPTMLYESALLQWALLKHn-----GLDEEAQFN-NIHNALSE 154 Helicobacter he...
CBG39869      86 ----SFeeeihwhlYGRFDFAggl-dgkPIKLLEFNADTPTMLYESALVQWALLKAn-----GLDENKQFN-NIHEAIGE 154 Helicobacter mu...
O25019        86 ----SFeeevhwhiYGRFDLA-GgldgkPIKLLEFNADTPTMLYETAVIQWALLKAn-----GYDENKQFN-NLYEALGE 154 Helicobacter py...
Q30QI3        87 ----SWendvhwhiYSRFDFAggv-dgaQIKLLEFNADTPTSLFETALLQWGLLKSn-----NMDESEQFN-NVYEAIKE 155 Sulfurimonas de...
WP_012855894  87 ----SWendvhwhlYGRFDFAggv-dgaPIKLMEFNADTPTSLYETAIIQWAMLKAn-----GMDEAKQFN-TVFEALKE 155 Sulfurospirillu...
WP_004509254  86 ----SWenevhwhlYSRFDLAggi-dghPIKLIEFNADTPTSLFETAIIQWAILKAn-----GLDEASQFN-NLYEALKD 154 Arcobacter butz...
BAF71572      87 ----SWendvhwhlYGRFDLAggi-ggtPIKLLEFNADTPTALFETTVVQWAILKQn-----GLKEAAQFN-ALYEALLD 155 Sulfurovum sp. ...
WP_015863974 159 SYRLlhc-------------dgEPYVVFTSHEDHMEDRETVRYLQRIS---GMEAAYVPLHELHVVaEdvplsgggvLRR 222 unclassified Ge...
WP_015843788 156 SLEYlgd------------gltAPYIVFTAHGDNVEDANTAAYLRSFL---PFPSSFVPLDQLQIIr-----------HA 209 Paenibacillus s...
Q7MSC7       155 NFKRmitlgddp--arfdevyeGWKILFSSIEGSLEEEMTMRLLEQIAresGFETAFSYVHEAKLD-E---------ESG 222 Wolinella succi...
Q7VK77       155 NFKRlitleddt--srfeelyeGWKILFSCISDSYEDITTTRFLEYIAkesGFICDFAPIDVINFS-A---------TEG 222 Helicobacter he...
CBG39869     155 NFKRmitlggdt--aafselyeGWKILFSSVRGNIEEERTTRFLQEIAqsvGFATAYAPMDAVQFS-A---------EEG 222 Helicobacter mu...
O25019       155 NFKRmvtlgedt--srfeemyeGWKILFSSVRGNIEEERTMRFLQDAAqsvGFETDFSYIDEVEFN-I----------EE 221 Helicobacter py...
Q30QI3       156 NFKRlvtlfedt--elfderydGWKILFSSIAGNDEEEATTRLLQQIAtdaGFNTGFEYLGDVKFD-----------EDG 222 Sulfurimonas de...
WP_012855894 156 NFKRlvvlegdt--eefaqyyeGWKILFSSIRGNIEDENTTRLLQSAAneaGFHTDFAYVDEVGFN-G---------SEG 223 Sulfurospirillu...
WP_004509254 155 NFKRiitlnsdiekfeeyysnlGWKILFSSISSSIEDINTTKLLQHIAseaGFNTDFEYIENVQFS-----------DDG 223 Arcobacter butz...
BAF71572     156 NCKRlvtldedv--sdfesrykGWKFLFTSIRGNAEEENTVKLLQHIAteaGYNTQFAYIDEIEFS-A---------EEG 223 Sulfurovum sp. ...
WP_015863974 223 GLYDAEGRRIDVLYRqTYPLEHLVEDRsaDGTKVGVQLlelwkereVALINPPSAFLLQSKAVQALIWGLYeegspffte 302 unclassified Ge...
WP_015843788 210 GLYDGEGNPIHVLYRqTFPVENLIEDEddEGNPIGLWLlelieqkkVAVINPPSAFLLQSKAVQAIIWGLYeegspffte 289 Paenibacillus s...
Q7MSC7       223 LFFND-QN-YEFWFK-LIPWENIAIDE----PEMALLIegmirnknTIFLNPAYTLLFQSKRMLKILWDLF--------- 286 Wolinella succi...
Q7VK77       223 VSHNG-IN-YEFLFK-LIPWENIAIDE----PELALLMsemmqnhnTIFLNPAYTLLFQSKRMLKILWDLF--------- 286 Helicobacter he...
CBG39869     223 VFFEE-EN-YEFLFK-LIPWENIAIDE----PELALIMqsmmenknTIFLNPAYTLLFQSKRMLKILWDLF--------- 286 Helicobacter mu...
O25019       222 GVFKN-GLNYEFLFK-LIPWENIAIDE----PELALLMqgmmenknTIFLNPAYTILFQSKRFLKLLWDRY--------- 286 Helicobacter py...
Q30QI3       223 IYDRD-DNQYEYWFK-LYPWEDIATDE----PELATMLttimqnqsAIILNPAYTLLFQSKGMLKILYDLF--------- 287 Sulfurimonas de...
WP_012855894 224 IFKDD-EN-FEYWFK-LIPWENIAIEE----GDLALLLdemvqeqkAIILNPAYTLLFQSKAFMKILWDLF--------- 287 Sulfurospirillu...
WP_004509254 224 IFKDD-EL-FEFWFK-LIPWEDIAIQE----SELALILteiikekkAIIFNPAYTLMFQSKAFMKVLWDLY--------- 287 Arcobacter butz...
BAF71572     224 IFYND-EN-YELWFK-LIPWEDIALEE----SDLAMLLtniikkqkAIIFNPAYTLMFQSKGMLKILWDLY--------- 287 Sulfurovum sp. ...
WP_015863974 303 EERQWiyehFLPTYLdeeyFLNQQMMYVQKPAFGREGDTVVIKngnGEpiavDSQQTYKdE------TPVYQQYAPLPET 376 unclassified Ge...
WP_015843788 290 EEHQWigehFLPTYLepdrFLEGRTKYVMKPAFGREGDTVKIFdgdGKvaaeDSHNSYAhY------LPVYQEYVELPTA 363 Paenibacillus s...
Q7MSC7       287 PNHSL----LLESSF----EPLKGKKQVKKHAFGREGESVQIW---SE----QGEILSEkAgaygnfPPLYQEFVEPNLH 351 Wolinella succi...
Q7VK77       287 PNHPL----LLPADF----SPLQNTKQVCKRAFGREGANVEIL---NT----QGQSIESkSgiygnhKPVYQAFYELNTH 351 Helicobacter he...
CBG39869     287 PNHPL----LLESSF----TPLKSKKQVKKTAFGREGANVEIL---DA----LGGVIAKnDgiyanhKPIFQEFYELNHQ 351 Helicobacter mu...
O25019       287 PNHPL----LLETSY----EPLANKKQIKKVAFGREGANSEIF---EA----SMQSLLKtDgvysnhKPVYQEFYELNSH 351 Helicobacter py...
Q30QI3       288 PDSPY----LLKSSF----EPLRGVKQVQKSVFGREGANIAII---DI----DGTVLQEqDgpyknhKKIYQEYVELNRD 352 Sulfurimonas de...
WP_012855894 288 PNHPL----LLETSF----EPLKNQKQVEKRAFGREGANTVIY---EH----DMSVIEKtEgeygnfKPIYQAYAELPKD 352 Sulfurospirillu...
WP_004509254 288 PNHPL----LLETSF----EPLKGKKQVEKRAFGREGANTKII---NA----DGSIDTQtSgdyeghKAIYQEYVEFPKD 352 Arcobacter butz...
BAF71572     288 PNHPF----LLETSF----DPLHGKKQVKKPFFGREGGNVSIL---DA----NAQPVESvEgdygnhKMVYQAYTELPAD 352 Sulfurovum sp. ...
WP_015863974 377 vvktvnGYetvKRLHTCFLINGKAVGIGLRAGGMITNNLSYYLPVMM 423 unclassified Geobacillus
WP_015843788 364 kfrtekGEregSYMYGSFLIGGKAGALGIRIGSPITDNLSYFLPVGI 410 Paenibacillus sp. JDR-2
Q7MSC7       352 ------QGl--FYQPNVFYAYE-ACALGFRRGGEILDNYAKFVSHYI 389 Wolinella succinogenes
Q7VK77       352 ------NGa--FYQPNVFFAYE-SCGLGFRKGGLIIDNYSKFVSHCI 389 Helicobacter hepaticus
CBG39869     352 ------DGf--YYQPNVFFAYE-SCALGFRKGGLVIDNYSKFVSHCL 389 Helicobacter mustelae 12198
O25019       352 ------NGl--YYQPNVFFAYE-SCALGFRKGGLILDNFSKFVSHRl 389 Helicobacter pylori
Q30QI3       353 s-----SGa--KYQAGVFFAYE-GAGVGFRKGDEILNNMSKFVGHVL 391 Sulfurimonas denitrificans DSM 1251
WP_012855894 353 e-----QGr--SYQAGVFFAYE-GCGLGFRRGGLIMENFSKFVGHRI 391 Sulfurospirillum deleyianum
WP_004509254 353 e-----KGd--CYQAGVFYAYE-ACGLGFRKGGKILNNMSKFVGHII 391 Arcobacter butzleri
BAF71572     353 t-----QGs--SYQAGVFYAYE-ACGLGFRKGGKILDNMSKFVGHIV 391 Sulfurovum sp. NBC37-1
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