3E20,3OBY,2QI2,3OBW,3MCA,2LLX


Conserved Protein Domain Family
eRF1_1

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pfam03463: eRF1_1 
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eRF1 domain 1
The release factor eRF1 terminates protein biosynthesis by recognising stop codons at the A site of the ribosome and stimulating peptidyl-tRNA bond hydrolysis at the peptidyl transferase centre. The crystal structure of human eRF1 is known. The overall shape and dimensions of eRF1 resemble a tRNA molecule with domains 1, 2, and 3 of eRF1 corresponding to the anticodon loop, aminoacyl acceptor stem, and T stem of a tRNA molecule, respectively. The position of the essential GGQ motif at an exposed tip of domain 2 suggests that the Gln residue coordinates a water molecule to mediate the hydrolytic activity at the peptidyl transferase centre. A conserved groove on domain 1, 80 A from the GGQ motif, is proposed to form the codon recognition site. This family also includes other proteins for which the precise molecular function is unknown. Many of them are from Archaebacteria. These proteins may also be involved in translation termination but this awaits experimental verification.
Statistics
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PSSM-Id: 397501
Aligned: 246 rows
Threshold Bit Score: 67.9263
Threshold Setting Gi: 74579973
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
3E20_H                23 RRLVKQliNCHGNGTSMITLIIPPGEQISRYSNMLAEEYGTASNIKSRVNRLSVLSAITSTRERLKLYNKvp-dNGLVIY 101 fission...
WP_008527563          19 RKVIEEleDYHGTGTQLVTIYVPDDTLISDVVAHVTQEHSEASNIKSKDTRTNVQDALKSIKDRLRYYDTkppeNGVVLF 98  Halorha...
WP_020446040          21 KKVIEEleEYEGSGTQLVSIYIPGGTRISEVQAHVTQEHSEASNIKSKQTRTNVQDALSSIKSRLQYYEEap-eNGMVLF 99  Salinar...
Q18FC0                18 RKVIEElrEYEGSGTQLVTIYIPPDRQVSDVVAHITQEHSEASNIKSKQTRTNVQDALTSIKDRLRYYDTyppdNGIVLF 97  Haloqua...
WP_009487297          18 QKVIEElkDYSGSGTQLVTIYIPPDKQVSDVVAHVTQEHSEASNIKSKQTRTNVQDALTSIKDRLRYYDTfppdNGMVIF 97  Halobac...
Q2FS61                17 KKMLERleAKEGSGTELISLYIPPDKQIFDVTAQLRDEFGQCANIKSKQTRTNVQSAISSILSRLKYYSKpp-eNGIAVF 95  Methano...
EHQ34948              18 KKTLEKlnEKEGSGTELITIYIPPDKQIYDVTNQLKDEYGQCSNIKSKQTKTNVQSAISSILSRLKQYNRpp-eNGLAVF 96  Methano...
WP_011752190          11 EKLVEElkKKEGRGTELVSLYIPAGRPISDVLNTLYYEYSTASNIKDRVTRHHVLDALATIINRLKLFREtp-pNGLIVF 89  Thermof...
Q74N59                10 KSLLRQlkSIEGRGTELVSLYIPSNYPIGKILDQLNYEYATAANIKDDTTRKNVQAALAKIINYLKDFKKip-eNGLVIF 88  Nanoarc...
goetting:Ngar_c16890  21 TKMINElsSISGHGTELVTVYIPPRRPIFDVIAQLRNESGTASNIKSDLTRTHVQDALSRTIEQLKLFKEtp-eNGLVIF 99  Candida...
3E20_H               102 CGEVIMEGN--KTRKLNIDFE-PFKPINTSQYLcDNKFHTEALAEL 144 fission yeast
WP_008527563          99 SGAIDAGGG--QTEMITRVLEsPPQPIQSFRYHcDSDFLLEPLEGM 142 Halorhabdus tiamatea
WP_020446040         100 SGAIDAGGG--QTDMVTKVLEsPPDPIESFRYHcDSNFLTGPLEYM 143 Salinarchaeum sp. Harcht-Bsk1
Q18FC0                98 SGAVSTGGG--QTTMVTRSLEsPPEPVQSFRYHcDSDFLTDPLEDM 141 Haloquadratum walsbyi DSM 16790
WP_009487297          98 SGAVDSGGG--RTDMVTEVLEsPPQPIESFRYHcDSAFLTEPLQEM 141 Halobacterium sp. DL1
Q2FS61                96 CGTVNKHGD--RQDLECDIVN-PPEPLNLYLYRcSSRFELEPLREM 138 Methanospirillum hungatei JF-1
EHQ34948              97 CGTVKLGGD--RTDLDCVIIE-PPEPLNLYMYRcSSKFELEPLQQM 139 Methanoplanus limicola DSM 2279
WP_011752190          90 AGYVAGDVPg-REKMEVHLIE-PPQKLNVWLYRcDSRFYTEILEEM 133 Thermofilum pendens
Q74N59                89 AGNIAGRGD---QDVRLWAIV-PPEKSNLKLYRcDSKFYVEPLEEM 130 Nanoarchaeum equitans
goetting:Ngar_c16890 100 CGAIPNDKGigKEKIEIYPVI-PPKPIQISLYRcDDHFWTDHLKEM 144 Candidatus Nitrososphaera gargensis Ga9.2
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