6K7G


Conserved Protein Domain Family
CDC50

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pfam03381: CDC50 
LEM3 (ligand-effect modulator 3) family / CDC50 family
Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w, and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.
Statistics
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PSSM-Id: 397450
Aligned: 298 rows
Threshold Bit Score: 153.107
Threshold Setting Gi: 341902003
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6K7G_C        70 VTSNNIREIEIDYTg-----------------------------TEPSspcnkc-----------lspdvTPcf----ct 105
EGT57938      86 AANQQVILIRFQYDnit------------------------------------------------------------ngy 105 Caenorhabditis ...
EFC48008      53 VTTQNVQEYRERYDqdc-------------------------tlDYTPsn-------------------lPGrgpycesv 88  Naegleria gruberi
CBZ12968      55 RANASAVDITVRYDhqqq-----------------------csfGYNNtgafry-----------easpgDVwqtgcvtd 100 Leishmania majo...
Q38DJ5        44 FCNRNAKEASFRYDnept----------------------kcteETFSlig----------------gqgTGkrtvceth 85  Trypanosoma brucei
Q4DU99        49 LMNKQAKEVTVRYDhihr-----------------------ctiTHNTgafiy-------------egnnMTfktgcmte 92  Trypanosoma cruzi
Q4CMM3        54 KASDSVFELSIRYD-------------------------------ETNnyqyrvgpddkyphkfrfnnsnFStga--hvs 100 Trypanosoma cruzi
CAM65930     168 VKGREYHIVEQEYShihqyqyvpsnssvninhgilqftadgvthAQGT----------------------RTw------- 218 Leishmania infa...
Q382L6        80 ITCNGHYEVRGNYQhihkyqyipsdpkvninegirsfyvgnevhRQGT----------------------RTr------- 130 Trypanosoma brucei
XP_001562722 172 VKGRQQHIIEQEYShihqyqyvpsntsvninqgilqftadgvthAQGT----------------------RTw------- 222 Leishmania braz...
6K7G_C       106 INFTLEKSF---------------EGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDS--SallnPSk-eCEPYrr----- 162
EGT57938     106 IDINITNFIpvsrflncsfsiffaQAPVYFYYELRNTFQMHRSLNQAYCKRQLVf---------GDstgCDSFknskyac 176 Caenorhabditis ...
EFC48008      89 TSITVTKRM---------------EPPIYMYYSLENFYQNHRRYTQSRSDSQLAGDNtiTp--tSAnsdCYPIvfygpdq 151 Naegleria gruberi
CBZ12968     101 VPFRVDKHL---------------KAPVYVYYGLENFYQNHRRFSNSKSDAQLAGQRv-Sa--aAIasaTSPLtypgelr 162 Leishmania majo...
Q38DJ5        86 FEFVLAETL---------------RQPVYFYYGLTKMYQNHRRYTNSRSGKQLMGADv------RSetdANPFvipgdtm 144 Trypanosoma brucei
Q4DU99        93 VSFDITEKL---------------KAPVYLYYELTRFYQNHRRYSISRNDEQLAGKAv------RYlpdTSPLaipgdiy 151 Trypanosoma cruzi
Q4CMM3       101 RNFTLLKSL---------------ASPIYLQYALVGFYQNYRRYAFSRDLAQREGNAy------LVsaaCEPFrfpgeyq 159 Trypanosoma cruzi
CAM65930     219 LEINISHRM---------------KAPVYMYYKIANMHQNHRDFHNGRSNSQLRGKS--Ki--dKAy-lCKPYtypgfrs 278 Leishmania infa...
Q382L6       131 VSFKLEKPM---------------KKPVYLYYTLGNFHQNFRAFHEGRSLDMLRGHR--Si--iGSypeCQPYerpgtin 191 Trypanosoma brucei
XP_001562722 223 LEINISRGI---------------LAPVYMYYKISNMHQNHRDFHRGRSNSQLRGKS--Ti--dKTy-tCQPYtypgfrn 282 Leishmania braz...
6K7G_C       163 --nE------------------DKPIAPCGAIANSMFNDTLe------------lFLIGNDS------------------ 192
EGT57938     177 enpSpsstflag--fstfcangQKFYAPVGGISSIMFNDYF--------------K---L-------------------- 217 Caenorhabditis ...
EFC48008     152 anlTgls------------tnaNMTYSPCGLIAWSMFNDTIsly--------gpnNSLVCDGlr---------------- 195 Naegleria gruberi
CBZ12968     163 htaDqginllg-----tflhysDFVYVPAGLIPWSMFNDTFtly--------ritHHEAAAVtapslrlicng------- 222 Leishmania majo...
Q38DJ5       145 dntNkpiefgg-----rnhtykDFVYVPVGLVAWSMFNDTFtlf--------rkeKNGSNPGevlicngtdfs------- 204 Trypanosoma brucei
Q4DU99       152 gisGtpikyvd----gsdlrykDFLYVPAGLIAWSIFNDTFtly--------teaTNGGTSRklicnatdfs-------- 211 Trypanosoma cruzi
Q4CMM3       160 nrkI------------------LGIYFPCGSIAWSLFNDSFsly--------rlsDASKSTInsqdfenaklic------ 207 Trypanosoma cruzi
CAM65930     279 degDtpititdvtgtqvtrparYFTYNPCGIAPWSKFNDTFilyrkltpaevvqaNISGVPVlhggvdsttpvtlicngt 358 Leishmania infa...
Q382L6       192 kaeKtevkvvvdg-envtlkyeEFLYNPCGIAPWSMFNDTFvlyrsrdvssaqndSVKLDEGaelicntsdfg------- 263 Trypanosoma brucei
XP_001562722 283 nkgEtpititdatgrqvtrparYFTYNPCGIAPWSKFNDTFvlyrkltpaevlqaSISGVPVlhggvdgttpvelicngt 362 Leishmania braz...
6K7G_C       193 -------------YPIPiaL-KKKGIAW----------------WTDKnV--------------KFRnP----------- 217
EGT57938     218 ----------------T--L-NNTEISWteegvivdkrretffqPEDSdN--------------LCDa------------ 252 Caenorhabditis ...
EFC48008     196 ------------hSEVS--NcTKKGIAW----------------SSDVdI--------------KFRpp----------- 220 Naegleria gruberi
CBZ12968     223 --safsrftneplDGAG--RcHKKGIAW----------------TSDVeF--------------KYKkPhfpppss---- 264 Leishmania majo...
Q38DJ5       205 ---rhtnkplhrsVSHN--HcDKNGIAW----------------ESDIkK--------------KFLePkwdgsg----- 244 Trypanosoma brucei
Q4DU99       212 ---kgnnlplngsESKN--McVKKGIAW----------------DTDVeY--------------KFKaPdleakn----- 251 Trypanosoma cruzi
Q4CMM3       208 -dggafdaagkslNEKN--LcIKKGIAL----------------SSDVrLfhpltdgkkdsavwRFGg------------ 256 Trypanosoma cruzi
CAM65930     359 dfglrgeplsgsvTKNH--C-SKMGISW----------------KADReI--------------RFRnItlredwwslyy 405 Leishmania infa...
Q382L6       264 ---ptgeplyqskTPNK--C-KKKGISW----------------PADEkI--------------RFRpLerdkklwslry 307 Trypanosoma brucei
XP_001562722 363 dfglkgepldgsvAVNR--C-SKMGISW----------------KADRnI--------------RFHnMtlredwwslyy 409 Leishmania braz...
6K7G_C       218 ----------P---GG--DNleerfKGTTKPVnwlkpvymld--------sdpdnngfiNEDFIVWMRTAALPTFRKLYR 274
EGT57938     253 ------------------KEf----RNTVKPIgwnqhic--------------emggyrNISFIKWLEPSTNKNFKKLYR 296 Caenorhabditis ...
EFC48008     221 -----------------kSPv----FNRITPTeyygepg-------------hllpsvtDEDFIVWMRTAALPTFRKLYR 266 Naegleria gruberi
CBZ12968     265 ----prpvwsApkwAYeaADg----DVNPNPPsrmpsdnayfnegwyadepghripvttDEDLMVWARVASLPKFRKLYR 336 Leishmania majo...
Q38DJ5       245 -----pvwtaPr-sEY------------GKPSiesndtyfnngwy--ageeghmipvvtDEDFMVWMRASPLPNVRKLYR 304 Trypanosoma brucei
Q4DU99       252 -----rfwtaAk-eLYt--------GKVPTPElsnddffnkgwy---agelghaipvttDEDFMVWMRPASLPSFRKLHR 314 Trypanosoma cruzi
Q4CMM3       257 -----------------------------DPAandpyqkegyyy----eepghpipsnvDEDYIVWSSLSYMKDFTKKYR 303 Trypanosoma cruzi
CAM65930     406 pypttneylrNg--WYlhEPg----HALPDPS---------------------------DYDLHVWMRASFTSNFRKLYR 452 Leishmania infa...
Q382L6       308 phkndnvyltNg--WYadEPg----HRLTDPE---------------------------DYDMQVWIRAAFLSNFSKLFR 354 Trypanosoma brucei
XP_001562722 410 pypttneylrNg--WYldEPg----HSLPDPS---------------------------DYDLQVWMRASFTSNFRKLYR 456 Leishmania braz...
6K7G_C       275 LiErk--sDLhptlPAGRYSLNVTY-----NYPVHYFDGRKRMILSTISWMGGKNPFLGIAYIAVGSISFLLGVVLLVIN 347
EGT57938     297 IlDsskhdGL----RRGVYRLYIDNvynpgEFKSKEFKLEKYFWILHPSWFGTEQKFLEIMYLIVGAGLLAFSCFLVGFQ 372 Caenorhabditis ...
EFC48008     267 IiNv----PL----EAGTYSFKIQQ-----RFNVSTFEGKKYVVITNNSWIGGRNMFLAIAYLVVGGLSFILACIFAIGA 333 Naegleria gruberi
CBZ12968     337 ViDe----DL----VPGTYLMRIQE-----HFNAASYGGTKSFSLATLSWLGGRNTFMAWMYFTIGAVSAVSGASFLCIH 403 Leishmania majo...
Q38DJ5       305 IiRt----DL----RAGKYVMKIRQ-----NYNTKPYGGEKSFIFLQPSMLGGKLTSLSITYFAVGGLALIFTVLVLFAS 371 Trypanosoma brucei
Q4DU99       315 ViNv----DL----PPGKYVMVIGE-----HFDVSSFGGTKSFALATLSFLGGKNVWLEALYFSLGGFSVVFAVVLLLVH 381 Trypanosoma cruzi
Q4CMM3       304 IiTt----DL----VPGNYLIDIVE-----NFDVFSFSGEKYVSLVTRSWIGGKNYVLGILFLLMGCISFVLSLSFIIVQ 370 Trypanosoma cruzi
CAM65930     453 IiHv----PL----HPGTYLVDISE-----FYDVVSFRGRKSVVLQHANWVGGRNIVLGVVFIIMGCASFVLGVTFTVEC 519 Leishmania infa...
Q382L6       355 IiDe----DL----REGNYFLDIEE-----FFDVTTFHGTKGYLLRTSSMFGRSGTLFAATFLIVGSVAFVVGVAFAIQY 421 Trypanosoma brucei
XP_001562722 457 IiDm----PL----YPGTYLVDISE-----FYDVVSFRGRKSVVLQHTNWIGGPNIGLGVIFILMGCLSFILGVTFTVEC 523 Leishmania braz...
6K7G_C       348 HK 349
EGT57938     373 IF 374 Caenorhabditis brenneri
EFC48008     334 II 335 Naegleria gruberi
CBZ12968     404 HW 405 Leishmania major strain Friedlin
Q38DJ5       372 HV 373 Trypanosoma brucei
Q4DU99       382 RF 383 Trypanosoma cruzi
Q4CMM3       371 YL 372 Trypanosoma cruzi
CAM65930     520 ML 521 Leishmania infantum JPCM5
Q382L6       422 CM 423 Trypanosoma brucei
XP_001562722 524 LL 525 Leishmania braziliensis MHOM/BR/75/M2904
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