Conserved Protein Domain Family
zf-ZPR1

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pfam03367: zf-ZPR1 
ZPR1 zinc-finger domain
The zinc-finger protein ZPR1 is ubiquitous among eukaryotes. It is indeed known to be an essential protein in yeast. In quiescent cells, ZPR1 is localized to the cytoplasm. But in proliferating cells treated with EGF or with other mitogens, ZPR1 accumulates in the nucleolus. ZPR1 interacts with the cytoplasmic domain of the inactive EGF receptor (EGFR) and is thought to inhibit the basal protein tyrosine kinase activity of EGFR. This interaction is disrupted when cells are treated with EGF, though by themselves, inactive EGFRs are not sufficient to sequester ZPR1 to the cytoplasm. Upon stimulation by EGF, ZPR1 directly binds the eukaryotic translation elongation factor-1alpha (eEF-1alpha) to form ZPR1/eEF-1alpha complexes. These move into the nucleus, localising particularly at the nucleolus. Indeed, the interaction between ZPR1 and eEF-1alpha has been shown to be essential for normal cellular proliferation, and ZPR1 is thought to be involved in pre-ribosomal RNA expression. The ZPR1 domain consists of an elongation initiation factor 2-like zinc finger and a double-stranded beta helix with a helical hairpin insertion. ZPR1 binds preferentially to GDP-bound eEF1A but does not directly influence the kinetics of nucleotide exchange or GTP hydrolysis. The alignment for this family shows a domain of which there are two copies in ZPR1 proteins. This family also includes several hypothetical archaeal proteins (from both Crenarchaeota and Euryarchaeota), which only contain one copy of the aligned region. This similarity between ZPR1 and archaeal proteins was not previously noted.
Statistics
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PSSM-Id: 397443
Aligned: 295 rows
Threshold Bit Score: 81.3897
Threshold Setting Gi: 74621925
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q8IDC0           399 SNCPCCn-HMGMNNFCEI-NIPGFKKCLILSFVCPNCNFKTSEIKSSGEINPKG-KKITL--TVNNKNDLNRFVIKSETA 473 Plasmodium ...
CBRAS:TCELL_0641  18 ARCPLCgkE--GVVEEFLyKIPFFGEVLLSTFKCPHCGYKHSDVMSLEESEP---VQILY--RVEKPGDERALFIKSSAA 90  Thermogladi...
Q6L1S2             7 vICPVCgkN--LYYTGSQvNIPYEGNIIIETYFCKSCGYHNSYTNTLEE--PKGhKRLKL--KIRNREDLKTIVYRSSKA 80  Picrophilus...
EQB69995          20 TPCPSCgeP--LFFIFYRtKITYEEAVEIETFFCKKCLYKSTQIRQIDRQEP---KTLVF--QVRNRDDLRTVLYRSPEA 92  Thermoplasm...
EQB68689          15 VKCPVCgeR--LYFIQMMtSIAYEKQILIQTYFCKKCLFKKNEVTQIESGEP---VRESL--LIRNMDDLRVVVYRSPQA 87  Thermoplasm...
EQB64418          20 VECPTCseK--LFLVYYTtEISYERSIYIQSYVCHKCLYKESEVFQLESGEP---VRVTM--GVDLPEDHGALLYRSPTT 92  Thermoplasm...
EQB65063          20 IECPVCssK--LFLVYYTtEISFEREIYIQSYVCHNCLYKESEIFQLNTGEP---IRVTM--YVKSPEDLGILLYRSPST 92  Thermoplasm...
jgi:Igag_0093     20 ITCPLCk-KDAILSLYEY-HLSLDESIVIMTIKCPNCGYKDNEIFSEGSKE----YNMCIelKVENDVDLNTLIYINPGT 93  Ignisphaera...
Q8SVQ9            22 MKCASCgwS---GKMKIV-DLGDVTENVICAFVCEKCGDKSVNFfek-mcdKRGsVRIEC--NFDSTEDLHREVNLSQLA 94  Encephalito...
EIJ88999          27 QPCVACg-NAGLLRLLIV-PDMFFPDSLISTFTCRVCPFRNKQMDEMNQSN-KG-VRISC--YLDKPEDLKRYLIIPSKA 100 Nematocida ...
Q8IDC0           474 SIN-IPV-VELTSDYG-TLGGTLTTVEGLIMKIIESLeekf----------kflLGDSNINthqyenentpnavNNnvDt 540 Plasmodium ...
CBRAS:TCELL_0641  91 TIK-IPE-VDIEVSPGlFSQGEITTVEGVIMRVIDVLky-------------------------------------acEn 131 Thermogladi...
Q6L1S2            81 DII-IPE-IDAEITHAsNTTGYITTVEGIIYRIKDHLdl--------------------------------------mG- 119 Picrophilus...
EQB69995          93 RIS-IPE-LEADIEPGeISTGEITTVEGILSQILDKLqll-----------------------------------deeDa 135 Thermoplasm...
EQB68689          88 RVV-IPE-IGAEIDPGeISDGEVTTVEGVVTRMWERMysal---------------------------------ldfeG- 131 Thermoplasm...
EQB64418          93 TVL-IPE-ISAEIYSGeNSNGGIISIEGLLGDIEDRFktmi---------------------------------ldfdG- 136 Thermoplasm...
EQB65063          93 TVL-IPE-IAAEIYPGeNSEGGIISIEGLLDSLRERFntmi---------------------------------qdfeG- 136 Thermoplasm...
jgi:Igag_0093     94 MVE-LRD-LGISIEIYqLDIGHIVTTEALILHIIDVIentc------------------------------------igs 135 Ignisphaera...
Q8SVQ9            95 SVEiTSEnLSFKLs---STYPSIQNVESFLIQGKDQIknlcgkeditsgacgkvLGDSSVS------------------- 152 Encephalito...
EIJ88999         101 KVS-FESgLD-GVTYT-HQEDSVSTVESLIRSIFEKLlsistlpe----------------------srltkeeLLelE- 154 Nematocida ...
Q8IDC0           541 TSYKIRELI-KKLYKLcKTeEfCPYDLIIDDIASNSYIS 578 Plasmodium falciparum 3D7
CBRAS:TCELL_0641 132 SSPACSSKI-EYLEGV-LRgL-KPVTIVVSDPTGVSRIl 167 Thermogladius calderae 1633
Q6L1S2           120 DGEEINYLH-QRIDGI-LNgPeEEVTLILDDVSGLSRIN 156 Picrophilus torridus
EQB69995         136 SPEDLRVIR-ERIEGI-IQgSgELFTFVIDDPMGKSRIN 172 Thermoplasmatales archaeon A-plasma
EQB68689         132 TENEEKAAR-KKMDMI-EKwQiFPFTLVMEDESGMSRIQ 168 Thermoplasmatales archaeon Gpl
EQB64418         137 DRENMDKIL-RFFDAV-NKgEhLVFTLILEDKSGKCRVN 173 Thermoplasmatales archaeon E-plasma
EQB65063         137 DQEELKKIL-SFFDAI-EKkEdESFTLILEDKSGKCRIN 173 Thermoplasmatales archaeon E-plasma
jgi:Igag_0093    136 QDNTCAHII-EALNDV-IKsK-KSISIVLRDPKGVTRIl 171 Ignisphaera aggregans DSM 17230
Q8SVQ9           153 -KETCEKKL-DDIQNL-INnP-K-FKMTINDDFGLSRva 186 Encephalitozoon cuniculi
EIJ88999         155 EYSGVATFLqDSMDNLnM-------TLSIDDPKGVArvm 186 Nematocida parisii ERTm3
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