8PN9


Conserved Protein Domain Family
OST48_N
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pfam03345: OST48_N (This model is not part of the current CDD release)
OST48, N-terminal domain
Members of this entry are involved in asparagine-linked protein glycosylation. In particular, dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST), also known as oligosaccharyltransferase, transfers the high-mannose sugar GlcNAc(2)-Man(9)-Glc(3) from a dolichol-linked donor to an asparagine acceptor in a consensus Asn-X-Ser/Thr motif. In most eukaryotes, the DDOST complex is composed of three subunits, which in humans are described as a 48kD subunit, ribophorin I, and ribophorin II. However, the yeast DDOST appears to consist of six subunits (alpha, beta, gamma, delta, epsilon, zeta). The yeast beta subunit is a 45kD polypeptide, previously discovered as the Wbp1 protein, with known sequence similarity to the human 48kD subunit and the other orthologues. This is the N-terminal domain of OST48 subunits from animals and plants and the orthologue from yeast Wbp1. This domain is distantly related to the IFT52 N-terminal domain (pfam23355).
Links
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Statistics
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PSSM-Id: 515526
Aligned: 106 rows
Threshold Bit Score: 173.936
Created: 13-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
8PN9_G        46 RTLVLLD--N-LNVR--ETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFI 120 human
Q5KAG9        27 RVLVVLE--S-AVTK--DDYSQFWNSLQQRGFELTFKEPESKDADLVKFGEAQYDHLIMFAPTAKNFSPSISPKAILDSQ 101 Cryptococcus ne...
EKC99562      28 RVLLVVDkeDrAQFKgeGTYTKFLDSLKSRGYQVQIKSADAKDLVLSSFGEPEYDHMVVMA-----------SKAILDAQ 96 
CCA72385      27 RVLVVLE--P-ALSK--TDYSTFFDGLEERGYQLTFRTPKQDKPLLVEYDVPNYDHVILFAPTTKTLSSDISPQSLLQLL 101
6_pfamImport   1 SVLVLLD--P-SLEK--ENFSIFFNGLEQRGYALTFRAPKDAEPAIAEYDVPSFSHVILFTPESKSYAQDVTPQSLVGLL 75 
ESK88302      27 SVLVILD--P-A-KR--ADYSIFFGGLEARGYELTFRSPKDEAPLLIEDDLPKFAHVVFFATDTKNFAKDITPQALVQLL 100
EPT06254      26 SVLIVLD--P-DLYI--ENFSRFFDGLEARGYDLTYRRPKDVYPPVIEHDHPKYAHVIFFAPEVKTYSQDITPQSLVTLL 100
EMD34356      27 SVLVLLD--S-SVNK--DDYSLFFKGLEERGYDLTFRSAKADEPAIIKDDHPQFSHAIIFAPETKSYPKDITPQSLVELL 101
XP_007396348  25 SVLVVLD--S-SLDK--EAYSVFWDGLVDKGYSLTFRSHKDARPAVVEDEVAQFNHVVVFAPDSKNLPQDLTPQSLVALL 99 
XP_003036270  27 SVLVVVD--P-K-NR--DLYTTFFNGLESQGYQLTFRAPKDEAPLIIQDDYPTFAHVVYFAPDTKTPAKDITPQTLVELA 100

8PN9_G       121 DGGGSVLVAASSdIGDPLRELGSECGIEFDEEKTAVIDHHNYD-IsdlGQHTLIVADteN---------LLKAPTIVGks 190 human
Q5KAG9       102 FKGLNALYILSPqLPEVQREHLREYDLEFIPSDLVLIDSFSHPsG---ESASTVLLP--PtecavsngaVLSDTTLt--- 173 Cryptococcus ne...
EKC99562      97 ATHLNTLYVLSPdMDDLLRSTLREYDVEPSPAGSELLDAFSHVgG---GSLSDVVL--------------PADSVVAPkp 159
CCA72385     102 SQKVNLMVALSP-KTTPMHSFASEFGLILPPPETPLFSHFPARk----GPRTTLEV-------------PLNPDSSvvk- 162
6_pfamImport  76 SQNTNLLIAMSP-KQTLLTSLAAEFSLILPPPGTPLISHHPARa----EPPTVIPV-------------AVPQSPlvr-- 135
ESK88302     101 SGNTNLLIALSP-KQNLITSLAPEFSLILPPPNTPLISHFPERs----EPANVIPI-------------STPKPSPETps 162
EPT06254     101 SKNTNLLIAVGT-KATPLTSLAAEFSLILPPPGTPLVSHFPERd----TPATVVPV---------------NVSNPvvs- 159
EMD34356     102 SKGTNLLLALSP-KQTPLTSLASEFSLIPPPPGTPLVSHFPERd----TPATVIPV-------------NVSHSSPilt- 162
XP_007396348 100 DKGTNLLLALSSkKQTTINTLASEFSLVLPPPGTPLISHFPARd----TPATVVPV-------------PPPHAHPvlt- 161
XP_003036270 101 KQNTNLLIALSP-KQTPISTLAAEFSLVLPPPGTPLISHFPERn----GPRTVVPI-------------APPQGNPivt- 161

8PN9_G       191 ----sLNPILF-RGVGMVADPdNPLVLDILTGSSTSYSFFPDKPITQY-------P------H-AVGKNT-----LLIAG 246 human
Q5KAG9       174 -----GGPIAYpSGTVHSAGL-NPYLIDVLHAPKKSYIGQDKILNADEierekavDgkkskdAlQTGKKA-----SLVSA 242 Cryptococcus ne...
EKC99562     160 vvpdsLGPIVFpHGVTVQLGE-NPYLLPLIVAPGTSYLGKSNGK--------------------QIGGTAphssgAAVAA 218
CCA72385     163 ---stSSPILF-SGINFGIST-NPMLFSIVNAPAESFATDTTKEEE-AeiliesaNrggeglW--AGSAM-----SVVAG 229
6_pfamImport 136 ---pgTPPVWF-SGVPHALNT-NPNLVPILRAPSESFAADSNDDSG-AsmlvdatEkggeglW--AGSQM-----DLVTG 202
ESK88302     163 iltpsTSPVWF-SGIPFALGQ-NPLLFPILNAPAESFAADA--DAT-VdvladaaEkggeglW--AGSQL-----AVAAG 230
EPT06254     160 ---edLPPVWF-SGVPFAFGN-NPHIVPILNAPPQSFAADSDSDSG-AdalvdaaNkggeglW--AGSSL-----GLVAG 226
EMD34356     163 ---peLPPVWF-SGVPFALGT-NPLLVPLLHAPAESFATDTLTDSD-AdviaeasEkggeglW--AGSQL-----GLVAG 229
XP_007396348 162 ---agLPPVWF-SGVPFAYTP-NPLLLPLLNAPAEAFAADTTSDAG-AdavveaaDrageglW--AGSSL-----ALAAG 228
XP_003036270 162 ---edLAPVWF-SGVPFALGN-NPLLVPVLNAPAHSFATDTETDAG-AsavvdaaGkggeglW--AGSSM-----GVVAG 228

8PN9_G       247 LQARNNARVIFSGSLDFFSDSFFNSAVQKaapgsqrYSQTGNYELAVALSRWV 299 human
Q5KAG9       243 LQTRDNARAGFVGSGEVFSDKYWGQTVETid---gkKVETGNAAFVTDFTKWV 292 Cryptococcus neoformans
EKC99562     219 MQNRQNVRIGFAGSKEMLSNEWWGKKLDG--------KETGNRKAIEDLTKWL 263
CCA72385     230 FQTKSGARVVWAGGVDMFSNDFASSTLAG-------GKKSGNAAVAQDIAKWA 275
6_pfamImport 203 FQTSHNTRVVFAGGVELFSDEFTKKELPN-------GKPSGNAQFARDVVNWV 248
ESK88302     231 FQALGGARAVWVGGIEMFSDEFAKKENEK-------GVKSGNQQFAQDVAAWA 276
EPT06254     227 FQTLGGGRATWVGGVELFSDEYAQKPAPN-------GGKSGNEQFASDVAAWT 272
EMD34356     230 FQPVGGARATWVGGVELFSDEFAHKEISS-------GVKSGNEQFTVDVASWT 275
XP_007396348 229 FQATTGARATFVGGVEMFSNEFAQKELPQ-------GKLSGNKQFAQDVASWS 274
XP_003036270 229 FQVVNGGRVMWVGGTEMFTDEFAQKELPT-------GGLPGNAQFVQDIARWT 274
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