5NM9,7QQE


Conserved Protein Domain Family
MH1
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pfam03165: MH1 (This model is not part of the current CDD release)
MH1 domain
The MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localisation signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx.
Links
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Statistics
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PSSM-Id: 515423
Aligned: 21 rows
Threshold Bit Score: 82.035
Created: 13-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
5NM9_A  33 AKRAVESLVKKLKDKr--DELDALITAVTSNgIQQ------------------SKCVTIA-------------------R 73  Trichoplax adhaerens
Q5H9N3 128 KVKWASRLLGKIKKDiqnDDKEAFISAINg--SEP------------------NKCI-IS-------------------- 166 Caenorhabditis elegans
Q02780  91 KQKWASRLLAKLRKDirpEYREDFVLTVTg--KKP-------------------PCCVLSn------------------- 130 house mouse
O15968 217 FREDFEALMKQLKRK----QRNELLLAVKSRlDPPtktqrdvvepttttaptyLQCILIPc------------------- 273 fruit fly
O57475  39 LRAAASAILKRLKEQt----LCVLLEAVESRgAAP------------------GGCVMVT-------------------R 77  African clawed frog
Q9YHB1  59 LKALAHCVLKKLKEKq----LEGLLQAVECKgGAR------------------SPCLLLPa------------------A 98  African clawed frog
O15105  92 LKALTHSVLKKLKER----QLELLLQAVESRgGTR------------------TACLLLP-------------------G 130 human
Q9W734 106 LKAVTYALLKRLKERs----LHSLLQAVESRgGTP------------------GGCVLVA-------------------R 144 chicken
O35182 173 LKTVTYSLLKRLKERs----LDTLLEAVESRgGVP------------------GGCVLVP-------------------R 211 house mouse
Q8T8C9  30 AEKSIRFLVNKKKPKl----TEDVEAAVM-QqTSK------------------TKCIVIYwsdwknhvrskldiggingS 86  Halocynthia roretzi

5NM9_A  74 TLDGRLQV-----------AGKKGFPHVIYS-----RIWRWPDL-HKNELKHIKLCKFA-FDLKLDH------VCVNPYH 129 Trichoplax adhaerens
Q5H9N3 167 -------V-----------ADQKGKMRRIDClrqadKVWRL-DLvTIILFKGIPLESTDgERLERSEacv-hpLCINPFH 226 Caenorhabditis elegans
Q02780 131 -------------------PDQKGKMRRIDClrqadKVWRL-DLvMVILFKGIPLESTDgERLVKSPqcsnpgLCVQPHH 190 house mouse
O15968 274 ------------------kTQTVWEPHVTAS-----RLFFWRELwNAKELKRLPTCPAArDCIy---------MCCNPLH 321 fruit fly
O57475  78 ---------------------HGPPPHLLLC-----RLFRWPELqHPGQLKALSGCQGA-GGSDNNSg----cCCCNPYH 126 African clawed frog
Q9YHB1  99 KLDSRLG------------QQAFSLP-LLLC-----KVFRWPDLrHSSDVKRLSCCDSY-GKNNPEL------LCCNPHH 153 African clawed frog
O15105 131 RLDCRLGPgapagaqpaqpPSSYSLP-LLLC-----KVFRWPDLrHSSEVKRLCCCESY-GKINPEL------VCCNPHH 197 human
Q9W734 145 G-ELRLG------------AARRPPPHLLLG-----KLFRWPDLqHPAELKALCECQSF-GAADGPT------VCCNPYH 199 chicken
O35182 212 A-DLRLG-------------GQPAPPQLLLG-----RLFRWPDLqHAVELKPLCGCHSFtAAADGPT------VCCNPYH 266 house mouse
Q8T8C9  87 YNNGSTDI-----------ASHKLHPHVWFC-----RLWRWPDLtSQVELRAIPSCECP-FDQDKDQ------ICVQPYH 143 Halocynthia roretzi

5NM9_A 130 YERVI 134 Trichoplax adhaerens
Q5H9N3 227 MAISV 231 Caenorhabditis elegans
Q02780 191 IGVSV 195 house mouse
O15968 322 WFRIL 326 fruit fly
O57475 127 YSRVC 131 African clawed frog
Q9YHB1 154 LSRLC 158 African clawed frog
O15105 198 LSRLC 202 human
Q9W734 200 FSRLC 204 chicken
O35182 267 FSRLC 271 house mouse
Q8T8C9 144 YSRVE 148 Halocynthia roretzi
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