6FZT


Conserved Protein Domain Family
MH1

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pfam03165: MH1 
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MH1 domain
The MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localization signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx.
Statistics
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PSSM-Id: 397329
Aligned: 19 rows
Threshold Bit Score: 83.5785
Threshold Setting Gi: 14194972
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6FZT_A  34 AEKAVDSLVKKLKKKk--GAMDELERALSCP-GQP------------------SKCVTIP-RSLDGRLQV---------- 81  human
Q21733  36 WALKVTRLLSRVAKKh--QCFEAFYDAVI-KgDPk------------------TRCCPAHnEKLIGNFG----------- 83  Caenorhabditis elegans
O15968 217 FREDFEALMKQLKRK----QRNELLLAVKSRlDPPtktqrdvvepttttaptyLQCILIPc------------------- 273 fruit fly
O57475  39 LRAAASAILKRLKEQt----LCVLLEAVESRgAAP------------------GGCVMVT-R------------------ 77  African clawed frog
Q9YHB1  59 LKALAHCVLKKLKEKq----LEGLLQAVECKgGAR------------------SPCLLLPaAKLDSRLG----------- 105 African clawed frog
O15105  92 LKALTHSVLKKLKER----QLELLLQAVESRgGTR------------------TACLLLP-GRLDCRLGPgapagaqpaq 148 human
Q9W734 106 LKAVTYALLKRLKERs----LHSLLQAVESRgGTP------------------GGCVLVA-RG-ELRLG----------- 150 chicken
O35182 173 LKTVTYSLLKRLKERs----LDTLLEAVESRgGVP------------------GGCVLVP-RA-DLRLG----------- 217 house mouse
Q02780  91 KQKWASRLLAKLRKDirpEYREDFVLTVTg--KKP-------------------PCCVLSn------------------- 130 house mouse
O17532 145 DQKACESLVKKLKDKk--NDLQNLIDVVLSKgTKY------------------TGCITIP-RTLDGRLQV---------- 193 Caenorhabditis elegans
6FZT_A  82 -SHRKGLPHVIYC-----RVWRWPDLQSHHELKPLECCEFP-FGSKQKE------VCINPYHYRRVE 135 human
Q21733  84 --------RAIMCv---lRAFRFPVIRYESQVKSILTCRHA-FNSHSRN------VCLNPYHYRWVE 132 Caenorhabditis elegans
O15968 274 kTQTVWEPHVTAS-----RLFFWRELWNAKELKRLPTCPAArDCIy---------MCCNPLHWFRIL 326 fruit fly
O57475  78 ---HGPPPHLLLC-----RLFRWPELQHPGQLKALSGCQGA-GGSDNNSg----cCCCNPYHYSRVC 131 African clawed frog
Q9YHB1 106 -QQAFSLP-LLLC-----KVFRWPDLRHSSDVKRLSCCDSY-GKNNPEL------LCCNPHHLSRLC 158 African clawed frog
O15105 149 pPSSYSLP-LLLC-----KVFRWPDLRHSSEVKRLCCCESY-GKINPEL------VCCNPHHLSRLC 202 human
Q9W734 151 -AARRPPPHLLLG-----KLFRWPDLQHPAELKALCECQSF-GAADGPT------VCCNPYHFSRLC 204 chicken
O35182 218 --GQPAPPQLLLG-----RLFRWPDLQHAVELKPLCGCHSFtAAADGPT------VCCNPYHFSRLC 271 house mouse
Q02780 131 -PDQKGKMRRIDClrqadKVWRL-DLVMVILFKGIPLESTDgERLVKSPqcsnpgLCVQPHHIGVSV 195 house mouse
O17532 194 -HGRKGFPHVVYG-----KLWRFNEM-TKNETRHVDHCKHA-FEMKSDM------VCVNPYHYEIVI 246 Caenorhabditis elegans
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