4B0T,4B0R


Conserved Protein Domain Family
Pup_ligase

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pfam03136: Pup_ligase 
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Pup-ligase protein
Pupylation is a novel protein modification system found in some bacteria. This family of proteins are the enzyme that can conjugate proteins of the Pup family to lysine residues in target proteins marking them for degradation. The archetypal protein in this family is PafA (proteasome accessory factor) from Mycobacterium tuberculosis. It has been suggested that these proteins are related to gamma-glutamyl-cysteine synthetases.
Statistics
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PSSM-Id: 397310
Aligned: 119 rows
Threshold Bit Score: 291.312
Threshold Setting Gi: 81662953
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
4B0T_B          11 RIMGIETEYGLTFvd-gDSkklRP---DEIARRMfrpive-----------------kysssNIFIPNGSRLYLDv---- 65  Corynebacteri...
WP_016483545     4 RIFGIETEFGCMV----RDpsvGT---PEEIVEQvkdyafydrryglidlhardyafeparaGGFLRNGGRLYIDa---- 72  Chthonomonas ...
FDU:OP10G_3407   8 RIFGVETEFGCLV----SDdtlGT---PESAVELvkdylfhelklgaidlharddvfepaqsGGFLMNGARLYIDa---- 76  Fimbriimonas ...
MA:Minf_1278    11 RIVGLETEYGCLF---------NQgtdLLSVPEQvkdylilekkmgli-dvhdrdydepagnGGFLFNGGRLYID----- 75  Methylacidiph...
BAM06375         5 SIIGLETEYGTLLn--rDPgsvRDlfrQEEIFQRlrdhifhtsrlgai-dqhqraydepvgnGGFLRNAGRLYVD----- 76  Leptospirillu...
WP_014961675     3 TVVGLETEYGALLs--gVSqgmRDlfrQEEMFQKvrdhifhvqrkgli-dqhqraydeppgnGGFLRNGGRLYID----- 74  Leptospirillu...
jgi:Psta_0514   11 RLVGLETEYALLrpataDNdgySR---FEVFRALvaslrtrip----------avaaqhfkeGVFHAAGGAVWFEteris 77  Pirellula sta...
Q8G3G3          28 RIFGVETEYGVAVt--gAErpvDA---GQVAMTMfqpivs-----------------rsrstNTYLANGSRLYLDv---- 81  Bifidobacteri...
kribb:BBB_0618  28 RIFGVETEYGVSVt--gSGhpvDA---AQVAMMMfqpvvs-----------------rarstNTYLTNGSRLYLDv---- 81  Bifidobacteri...
A1A0U7          28 RIFGIETEYGVSVt--gSDrpcDA---GQTAMMMfqpiva-----------------earstNTYIENGSRLYLDv---- 81  Bifidobacteri...
4B0T_B          66 --GSHPEYATAECDNLTQLINFEKAGDVIADRMAVDAEESlake-diaGQVYLFKNNVDSV-GNSYGCHENY-------- 133 Corynebacteri...
WP_016483545    73 -vGSHEEYATPECCDLLDLVRYDRAGRILLSRLLKDLql--------sPVVSFHNNSIDHFgGHTFGCHENY-------- 135 Chthonomonas ...
FDU:OP10G_3407  77 -vGSHLEYATAECRHLKDLVANDRAGQRQIVRAIKELgl--------dDALFVYNNSVDHFgGHTFGCHENY-------- 139 Fimbriimonas ...
MA:Minf_1278    76 --MGHVEYCTSECKNLADLVASDYAGEKLLQTALDELgl--------sSKVSFIKNNIDHYtGATFGCHENY-------- 137 Methylacidiph...
BAM06375        77 --MGHLEYASPEALDLLDLVLVDRAGDKILQDAINELgl--------tSEISLIKNNIDHQtLATFGSHENY-------- 138 Leptospirillu...
WP_014961675    75 --MGHVEYATPECHTLLDLVRADRGGDLLLQQAVDEMgl--------sGEISFIKNNIDHQtLATFGSHENY-------- 136 Leptospirillu...
jgi:Psta_0514   78 sgGGLVEGSSPECRGPMQVLIWQKAQDKLLAAALDDATD---------GETKLIKNDRDAF-GNVYGAQENYeatlatgw 147 Pirellula sta...
Q8G3G3          82 --GSHPEYATAEARDPREALAQDLAGEHVMRNLALKAQRKlresygahATIHVFKNNVDSA-GHAFGCHENY-------- 150 Bifidobacteri...
kribb:BBB_0618  82 --GSHPEYATAEARDPMDALLQDLAGERVMAGLALDAQARlrarygdgVNVHVFKNNADSA-GHSFGCHENY-------- 150 Bifidobacteri...
A1A0U7          82 --GSHPEYATAEARDPMDALALDAAGELVMRDLALDAQRRlrsiqgagSTVHVFKNNVDSA-GHSFGCHENY-------- 150 Bifidobacteri...
4B0T_B         134 ----------------------------------------------LVGR------------------------------ 137 Corynebacteri...
WP_016483545   136 ----------------------------------------------LVQL------------------------------ 139 Chthonomonas ...
FDU:OP10G_3407 140 ----------------------------------------------LVRG------------------------------ 143 Fimbriimonas ...
MA:Minf_1278   138 ----------------------------------------------LIHR------------------------------ 141 Methylacidiph...
BAM06375       139 ----------------------------------------------LVSR------------------------------ 142 Leptospirillu...
WP_014961675   137 ----------------------------------------------LVSR------------------------------ 140 Leptospirillu...
jgi:Psta_0514  148 rllawrasliaifpivlltwlslwiitgavmlyalaavlvylalerLVRRpqrlamllfgcsferlgeagptgpqwmeqf 227 Pirellula sta...
Q8G3G3         151 ----------------------------------------------LVRR------------------------------ 154 Bifidobacteri...
kribb:BBB_0618 151 ----------------------------------------------LVRR------------------------------ 154 Bifidobacteri...
A1A0U7         151 ----------------------------------------------LVRR------------------------------ 154 Bifidobacteri...
4B0T_B         138 --------------------S-MPLKALGKRLMPFLITRQLICGAGRIhhpnpl------------------------dk 172 Corynebacteri...
WP_016483545   140 --------------------EdRFFTDALSSLLPFLVTRQIFAGVGRVgghrltrpssknnimtlsehevdyvwvsnfyg 199 Chthonomonas ...
FDU:OP10G_3407 144 --------------------DdEFLNTSVQMLYPFLVTRQIFSGVGRVgghiltagglrpsyqevmenpvdyiwvshvys 203 Fimbriimonas ...
MA:Minf_1278   142 --------------------NaPLTQENVDSLLAFLAIRGLMVGSGRVgsvlssr----------------------kyn 179 Methylacidiph...
BAM06375       143 --------------------QfPFTEKGMEPLVAFLVTRQIFTGAGRVgsanlnes--------------------litf 182 Leptospirillu...
WP_014961675   141 --------------------DfPFTERGMEPLVAFLVTRQIVTGAGRVgvsyvnds--------------------lisl 180 Leptospirillu...
jgi:Psta_0514  228 lsvvvrviaaplagvllaiiAvTSFCEHRKKLLPFLASRAVIGGSGMI-------------------------------- 275 Pirellula sta...
Q8G3G3         155 --------------------F-VPLETIEHQLLPFLITRQLYTGAGR--------------------------------- 180 Bifidobacteri...
kribb:BBB_0618 155 --------------------Y-VPLETVEHELLPFLITRQLYTGAGR--------------------------------- 180 Bifidobacteri...
A1A0U7         155 --------------------F-VPLKTIEQELLPFLITRQLFTGAGR--------------------------------- 180 Bifidobacteri...
4B0T_B         173 gesFPL-GYCISQRSDHVWEGVSSATTR-SRPII--------------NTRDEPHADSHSYRRLHVIVGDANMAEPSIAL 236 Corynebacteri...
WP_016483545   200 veiDPTvNFQLSQRADHIVKTISSRVRF-NRAII--------------NPKWDSYYSYSNLHRLHVLFGESNMSEYALML 264 Chthonomonas ...
FDU:OP10G_3407 204 vvpDDRvPFQLSQRADHIIKTIASRVRF-NRALI--------------NPKWEHFYSHDGMTRLHLLFGESNQNEFAYAL 268 Fimbriimonas ...
MA:Minf_1278   180 kkeGKTvNFQVMQRADYIQTEIYEWVQF-NRALI--------------NARDEPLSDPCRFRRLHLLLGDSNILPFSQAL 244 Methylacidiph...
BAM06375       183 pptAKPvTFQLSQRADYVVNKFYQWVQM-NRSIV--------------NTRDEPLADPALFRRMHLLMGDSNMSQFATAM 247 Leptospirillu...
WP_014961675   181 dteRPPvHFQISQRADYIVNKFYQWVQM-NRSIV--------------NTRDEPLSDPLQYRRMHLLLGDSNMSQFATAM 245 Leptospirillu...
jgi:Psta_0514  276 ---DDQgRFLLADKATAMNCSIGFGGLLgDRPIFtfghfyktifsdawMAPGEYFRLFSPRQRLQIGLGDSNLCDVSQYL 352 Pirellula sta...
Q8G3G3         181 ---MTPdGFQITQRADFLDEAVSSATTR-SRPMV--------------NTRDEPHADPDSFRRLHVIIGDSNRSQWSTWM 242 Bifidobacteri...
kribb:BBB_0618 181 ---VTDqGFEITQRADYLDEAVSSATTR-SRPMV--------------NTRDEPHSDPDEFRRLHVIIGDSNRSQWATWM 242 Bifidobacteri...
A1A0U7         181 ---MGEqGFQITQRADFLDEAVSSATTR-SRPMV--------------NTRDEPHADPDAFRRLHVIIGDSNRSQWATMM 242 Bifidobacteri...
4B0T_B         237 KVGSTLLVLEMIEA------DFGLPSLElaNDIASIREISRDAT-GSTLLSLKDGt--TMTALQIQQVVFEHASKWLEQR 307 Corynebacteri...
WP_016483545   265 KVGTTCLVLDLIED------GIAPPDVEvaDPLETLRSVSRDPT-MKWIVRLRNGr--TISAIDLQRRYLDAAKRYLYRR 335 Chthonomonas ...
FDU:OP10G_3407 269 KVGTTVLVLRLLEE------HRVPERLTlaQPLIALREVSRDQD-FKWIVSLAEGg--SVRSVDLQREYLRLAEVYRGES 339 Fimbriimonas ...
MA:Minf_1278   245 KVGSTALVLDLLEC-------RKLPPIAftDPVKTMRQVSHVAS-ADLLIELADGr--KWTPVEVLSEYYLQAQKLEEKD 314 Methylacidiph...
BAM06375       248 KFGTTRLALSLIED-------GLAPAIGiiDPVRANHSISHDIS-MGWRVMDNDGq--MLTATDLQWMFLEAADRNYRGR 317 Leptospirillu...
WP_014961675   246 KFGMTKIVMDLVED-------GLAPAVGvrDPVQACRAISHDLS-MRWNLQDLEGr--ILSALDMQEMFLEKAVKAYSGR 315 Leptospirillu...
jgi:Psta_0514  353 RVGTTLLVLDMIEAg----eATNLPQLR--SPLRACRKFTSDPT-LQATAPLRGGg--EITALEVQRAYLEACRQFLAKH 423 Pirellula sta...
Q8G3G3         243 KLAVTHLVLCAIEDafrhgvPSGFEQYAfaDPAAANRTVSRFLDnPRAELTLESGe--SVSALGLQRRYYAAVKAFIETH 320 Bifidobacteri...
kribb:BBB_0618 243 KLAVTHLIICVIEEaarlgeDSGFASCAlaDPGAANRAVSADLTcVTARLRLADGs-ePVSAVQMQRRYQAVVRDFMERH 321 Bifidobacteri...
A1A0U7         243 KLATTHLVLCVIEQagregkDSGFARFSfaDASAANHKVSRDLTgVEASFDMADGtvmEGGAVAIQERYLEIVERFVGQH 322 Bifidobacteri...
4B0T_B         308 PEPEFsgtsntEMARVLDLWGRMLKAIeSGDFSEVdTEIDWVIKKKLIDRFIQRGNLGLd---dPK-LAQVDLTYHDIRP 383 Corynebacteri...
WP_016483545   336 DP---------QTDLVLREWETILDDL-ERDPLSTaDRLDWAAKFVLYRQYMEETGASWq---dDV-MQSLDLEYHNATP 401 Chthonomonas ...
FDU:OP10G_3407 340 T----------QTDWILDNWRQVLDDL-EKDPMAMeDRIDWVAKRKIVEEYMSEEGVGWn---dDA-LHSVDLEYHNIDP 404 Fimbriimonas ...
MA:Minf_1278   315 E----------DAYWILEAWEEVLEGL-SGNQEKLiGKVDWITKKYLLESFCMKENIAWd---dPW-LDSLDLEYHQIDR 379 Methylacidiph...
BAM06375       318 DE---------ETDWVISEWAALLSDLgQKDPEKVaDRIDWAAKYVLLDQFREDEGLDWs---dPW-LESLDLEYHNIHP 384 Leptospirillu...
WP_014961675   316 DE---------DTDWVLEEWGKILSDLaKKDPSLVtDRLDWAAKYNMISAFRESENLEWn---dPW-LESLDLEYHNIDP 382 Leptospirillu...
jgi:Psta_0514  424 PEAPE------EAFQVLSLWDETLKQL-AGDRQALvGSIDWVTKQRLIDE--NTAGVS------PKvLKKIDIKYHELSS 488 Pirellula sta...
Q8G3G3         321 GDALAgslpatTIDTIMGEWSRVLDALeRGAYDALaDRVDWAAKKCLFDALKRR----RpdvtfAQ-MEQLELDYHDIAN 395 Bifidobacteri...
kribb:BBB_0618 322 HDAVDdalprtDVDQILSAWSLVLDQLeASDVEALsDRVDWAAKYRLFQALKRRKGE-Vt---dAM-RERLDMDYHDIVN 396 Bifidobacteri...
A1A0U7         323 PE-VCsslprtDVHEVIRRWRRVIEAFrSGVSETFaDKVDWLAKRRLFDMLRNRAGGRLs---vSK-LEQLDMDYHDVAN 397 Bifidobacteri...
4B0T_B         384 GrGLFSVLQSRGMIKRWTTDEAILAAVDTAPDTTRAHLRGRILKAADTLgv--pVTVDWMRHK 444 Corynebacterium glutamicum
WP_016483545   402 EeGLFYGLEQAGLMRRLVTDEQVEEALTNPPENTRAYGRGLIVAQLLAHpg-lrYVIDWDAIY 463 Chthonomonas calidirosea
FDU:OP10G_3407 405 ErSLFYGLQEMGRTKRILDDLDITMAMTDPPQNTRAKGRAALVEQVLSRknprfYLFDWNGVa 467 Fimbriimonas ginsengisoli Gsoi...
MA:Minf_1278   380 KkNFVWLLPNTRYEDLNLPSWPQTNFIADPPRNTRAYVRSLLMKQLAKEkv--sYVIDWDYVQ 440 Methylacidiphilum infernorum V4
BAM06375       385 EkGLFRILEQEGRHRRLISDRQITDGVGKPPDHTRAYGRSIAVNHILEEnd-lsYIIQWFGIQ 446 Leptospirillum ferrooxidans C2-3
WP_014961675   383 AkGLFHILEEEGRHRRLIPPSISETGATCPPSFTRAEGRAQAIDRILENpe-mlYIIQWFGIQ 444 Leptospirillum ferriphilum
jgi:Psta_0514  489 E-GYFQKLLAVGLIRQLTTPAEVERAMRCPPSGTPAAVRGQFIREFASGdk--pITANWHYVL 548 Pirellula staleyi DSM 6068
Q8G3G3         396 G-RLYGSLVARNQMRELLTGDNVEYAVHNPPTDTRAALRGRFVDAALNVga--qFSADWTHLT 455 Bifidobacterium longum NCC2705
kribb:BBB_0618 397 G-SLYPSMLRRGVMRQLATPQQVEHAVSEPPADTRAALRGAFVAKALSTqc--rFSCDWTRLS 456 Bifidobacterium bifidum BGN4
A1A0U7         398 G-ALYASLCRRGAMRTLVNESQAHEAIDVPPHDTRAALRGRFIQSARSHna--qYSCDWTRLS 457 Bifidobacterium adolescentis A...
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