2D3J


Conserved Protein Domain Family
WIF

?
pfam02019: WIF 
Click on image for an interactive view with Cn3D
WIF domain
The WIF domain is found in the RYK tyrosine kinase receptors and WIF the Wnt-inhibitory-factor. The domain is extracellular and contains two conserved cysteines that may form a disulphide bridge. This domain is Wnt binding in WIF, and it has been suggested that RYK may also bind to Wnt. The WIF domain is a member of the immunoglobulin superfamily, and it comprises nine beta-strands and two alpha-helices, with two of the beta-strands (6 and 9) interrupted by four and six residues of irregular secondary structure, respectively. Considering that the activity of Wnts depends on the presence of a palmitoylated cysteine residue in their amino-terminal polypeptide segment, Wnt proteins are lipid-modified and can act as stem cell growth factors, it is likely that the WIF domain recognizes and binds to Wnts that have been activated by palmitoylation and that the recognition of palmitoylated Wnts by WIF-1 is effected by its WIF domain rather than by its EGF domains. A strong binding affinity for palmitoylated cysteine residues would further explain the remarkably high affinity of human WIF-1 not only for mammalian Wnts, but also for Wnts from Xenopus and Drosophila.
Statistics
?
PSSM-Id: 426564
Aligned: 28 rows
Threshold Bit Score: 126.178
Created: 26-Apr-2021
Updated: 29-Sep-2021
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
2D3J_A        15 WIDAHQARVLIG-FEEDILIVSEGKMAPFTHD-----FrkaqqrMPAIPVNIHSMNFTWQAAGQaeYFYEFlSLRSLDKG 88  human
XP_011725577 105 WIDAHQARVLIG-FEEDILIVSEGKMAPFTHD-----FrkaqqrMPAIPVNIHSMNFTWQAAGQaeYFYEFlSLRSLDKG 178 pig-tailed macaque
XP_015786501  52 WIDENQVREFAD-VRSQIYAIREGKLLPYLSDpnfqsY------LPTIPAEVISINFTWMSGNRktYHYSFeELRSFNRS 124 two-spotted spi...
Q16Q73        46 WINEQQVKIFSG-VPTKIYAIDNGRVSLHLRDpnfshY------LPIIPSEVSCVNFTWKSG-SkkYHYNFdRLVSEDEQ 117 yellow fever mo...
XP_001606619  45 WIDRQQIKMFSG-VEMEIYAIDEGRVVPYLLDpefesK------LPIIPNEVSYVNFTWKSG-VkkYNYDFyLLKSFDES 116 jewel wasp
EFA13018      42 WIDEKQVKMFSG-MSMEIYAIVNGNVLPYILDpnfekY------LPVIPSEVSYVNFTWKAG-MkkYYYNFdRLQSFDES 113 red flour beetle
XP_012543869  42 WIDERQVRMFSG-ISMQVFAIVNGHVSPYVLDpnfsnK------LPIIPSEVGYVNFTWKS--KkrYYYNFdTLKSSDLK 112 domestic silkworm
EEB17670      41 WIDSQQVKMFSGhFIGEIHVIDGGYVLPYILDpnfekY------LPVIPSEVNSVNFTWRSG-SkkYFYHFdILKTLDES 113 human body louse
XP_003241249  41 WIDEQQVKMYSG-LKMPIYAIMEGKVTPYILDknfeqY------LPVIPSEVGYVNFTWMSG-NknYFYVFdILDSDDKN 112 pea aphid
EFX65761      18 WIDQQQVKMFSG-FPMEIYIAAEGRVLSYILDpnfekY------LPVIPSEVGYVNFTWKSGEHkkYYYHFdRLQSFNED 90  common water flea
2D3J_A        89 IMadPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMN----SEGNTILQTPQ 145 human
XP_011725577 179 IMadPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVNVIVMn----SEGNTILQTPQ 235 pig-tailed macaque
XP_015786501 125 ILfdPTVSINREGIIPEEPGIFKVTILCSGTVPGVSSFAISLAITDnngnNLEGTPIRMRL 185 two-spotted spider mite
Q16Q73       118 ILkpPTISIKTSGRVPKNAKEFSVTLPCSGNVSGIAVFSIGLLIQSrkgkPVPGTPLRIKL 178 yellow fever mosquito
XP_001606619 117 ILktPSITINKKGRVPRRQKEFSVLLPCSGNNSGVAQFSIGLMIETrknkPLNGTPLRLSL 177 jewel wasp
EFA13018     114 ILeaPVISIKTKGRVPKRPKEFSILLPCLGNSSGVAPFGIGLLIESrkgkPLNGTPLRLKL 174 red flour beetle
XP_012543869 113 ILkaPVLSIKTQGRVPKTAKEFSIILPCVGNSSGVATFEIGLVLKNarglPLKGTPLRLNL 173 domestic silkworm
EEB17670     114 ILesPQISIKTRGKIPKRPKVFSVYLPCSGNRSGIAPFEVGLLIETrkgkPLVGTPLRLKL 174 human body louse
XP_003241249 113 ILepPTVTVKIDGKIPKRPKVFSILLPCSGNRSGIASFNIRLLIQNrkgrALPDTPLKVKL 173 pea aphid
EFX65761      91 ILqpPTVSVEIRGRVPRKPKVFSVLLPCSGNSSGVASFSIGLLIEShkgkPLPGTPLRLRL 151 common water flea
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap