1VK8,2EKY,1LXN,1LXJ


Conserved Protein Domain Family
Thiamine_BP

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pfam01910: Thiamine_BP 
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Thiamine-binding protein
The crystal structure of two of these members shows that this domain has a ferredoxin like fold and is likely to exists as at least homodimers. Sulphate ions are are located at the dimer interfaces, which are thought to confer additional stability. Although the function of this domain remains to be identified, its structure suggests a role in protein-protein interactions possibly regulated by the binding of small-molecule ligands. Solution of the structure of the hyperthermophilic anaerobic Thermotoga maritima sequence, UniProtKB:Q9WYV6, shows that this has a beta-alpha-beta-beta-alpha-beta ferredoxin-like fold and assembles as a homotetramer. It was possible to identify a pocket in each monmer that bound an unidentified ligand. It was also found that it bound charged thiamine though not hydroxymethyl pyrimidine. It is proposed that it is transporting charged thiamine around the cytoplasm. Under oxidative conditions this bacterium is under stress, and the transcriiptional unit within which this protein is expressed is up-regulated in these conditions, suggesting that the chelation of cytoplasmic thaimine is part of the response mechanism to such oxidatvie stress, which is mediated by this family.
Statistics
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PSSM-Id: 426506
Aligned: 82 rows
Threshold Bit Score: 71.3977
Created: 26-Apr-2021
Updated: 29-Sep-2021
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1VK8_D          18 VSIKVVPA-VE-DGRLHEVIDRAIEKISSWGXKYEVGPSNTTVEGEFEEIXDRVKELARYLEQF-AKRFVLQLDIDYKAG 94  Thermotoga ma...
Q97J88           7 VSLQVLPV-VE-EKRLYEVVDKVIEYIKASGVKYIIGPMETTMEGDLDTLLEIVKKAQEICVKEgSERVASVVKIDYKAE 84  Clostridium a...
Q8R9M3           6 LSLQVLPL-VP-EEKVYPIVDKVIKYIKSTGVNFMVGPMETTMEGELDILLDIVKKAQKICEEEgANRVISIIKLDYRSS 83  Caldanaerobac...
1_pfamImport     1 VSFEVLPGgLPdKATTYAAVDAAIAVVAASGLTYRVCPMETTIEGDYDAIMAVIKQAQEAVLAAgASRVFTLIKVDYDPN 80 
Q5E4V9          13 VAFQVIPR-LK-EGNNFEVVDKAIEVVKAANVPYQVGAMETTMKGELNYLLEVVKKAQQACYDAgALEVITNIKIHSKTE 90  Aliivibrio fi...
WP_011879496     6 VGFQVLPK-TK-GDNSYQIVDKAIEVVQKSGVKYEVGPMETVMEGELDVLIDIVKRAQEACITAgASEVMTYIKIHYRPE 83  Desulfotomacu...
tigr:GBAA_0380   7 MSFSVVPQaK--TKDVYSVVDKAIEVVQQSGVRYEVGAMETTLEGELDVLLDVVKRAQQACVDAgAEEVITSIKIHYRPS 84  Bacillus anth...
Q03H92           5 VAIQVLPImED-EKKLVAAVDQAIKYIKSTGVNYQVGAFETTLEGDYDQLMEIVKNITKITADAgAPQVMSYVKINYKPE 83  Pediococcus p...
jgi:Cphy_1150    5 VAIQTLPVaKN-DEDLIRIVDEVIAYIKSTGLNYYVGPFETAIEGDYDELMDIVKECQHIAIRAgAPSVAAYIKVTYRPE 83  Lachnoclostri...
Q24P05           6 VAIQVLPA-VE-GQEVIRVVDKVIEYLKSSGLNVYVGPFETTVEGEYDQLMEMVKHCQLICIEEgAPSVMSYVKISYKPE 83  Desulfitobact...
1VK8_D          95 -G--ITIEEKVSKYR 106 Thermotoga maritima
Q97J88          85 -G--VTMNEKVGKYK 96  Clostridium acetobutylicum
Q8R9M3          84 -G--VTMEEKIKNYI 95  Caldanaerobacter subterraneus subsp. tengcongensis
1_pfamImport    81 -G--SSIAEKLAKYE 92 
Q5E4V9          91 aA-----TDTFCTYD 100 Aliivibrio fischeri ES114
WP_011879496    84 -G--VTLDEKIAKYR 95  Desulfotomaculum reducens
tigr:GBAA_0380  85 -Tg-VTIDEKVWKYr 97  Bacillus anthracis str. 'Ames Ancestor'
Q03H92          84 -GqvLGIHEKTDKYQ 97  Pediococcus pentosaceus ATCC 25745
jgi:Cphy_1150   84 -GdvLTIEKKVTKHH 97  Lachnoclostridium phytofermentans ISDg
Q24P05          84 -GgiWTIDEKVTKHh 97  Desulfitobacterium hafniense Y51
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