Conserved Protein Domain Family
Rep_1

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pfam01446: Rep_1 
Replication protein
Replication proteins (rep) are involved in plasmid replication. The Rep protein binds to the plasmid DNA and nicks it at the double strand origin (dso) of replication. The 3'-hydroxyl end created is extended by the host DNA replicase, and the 5' end is displaced during synthesis. At the end of one replication round, Rep introduces a second single stranded break at the dso and ligates the ssDNA extremities generating one double-stranded plasmid and one circular ssDNA form. Complementary strand synthesis of the circular ssDNA is usually initiated at the single-stranded origin by the host RNA polymerase.
Statistics
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PSSM-Id: 426265
Aligned: 17 rows
Threshold Bit Score: 269.628
Created: 19-Apr-2021
Updated: 29-Sep-2021
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
CAL80011               105 GRRVRGPNECKHPLCPTAQLARQRRTYRRVAQALERYLRrkEgm--vGIMLTTTM-EsCPSHELRYRTGQLIAACSRLMT 181 Brady...
EFX41640                78 ALVLAWAIFCKIKWCPMCAWRKARKILGELLSIFQQIEQdhRv---aYVFLTLTTrN-APLSELRALSAHMSKSWHRLQQ 153 Helic...
EDX73127                52 VGFINNVHWCRCPRCPLCQWAKVGKWRAKFFQGLRRLYLenEv---nVIFVTLTSrN-CCIKGLRDELELMASAFNRLLS 127 Coleo...
jgi:Chro_5978           62 VFRLQAARFCRVRHCPICQWRRSLMWKARFIKALPSIAA--DhptarWIFLTLTVkN-CPLPELRENLTSMNKAWKRICD 138 Chroo...
Q1QGV9                  80 FRVLANGSFCRERLCPFCMKRNSAWLHVRLTQAVERFLKqnPqh--qAALLTLTMkN-MTEEKLRYWLDRLLKAFRKLMR 156 Nitro...
PRJNA230994:X953_19940  47 KKKQHKGNTCKNRFCPICAWKKSRKDALALGVQMEFIKQehQk---dFIFLTLTTpN-VAADQLENEIKTYNHAFQKLMQ 122 Virgi...
WP_016252524            61 QLRLHQAWFCKSKLCPICNWRRSMKYGVQLGQIIDVARE--RepkakFIFLTLTVkN-VSGEKLGEELTNLTKSFDRLFR 137 Enter...
WP_012655909            71 YLKLYKTWFCKSKLCPVCNWRRAMKNSYQAQRVVEEVIK--EkpkarWLFITLSTkNaVDGQTLNKAVSEMTKAFDRLMR 148 Macro...
WP_046316098            64 RLKLFQTWFCKSKLCPLCNWRRSLKSSNQLQKLLQVALQ--KepqgrFLFLTLTQeN-STGETLKQDISRMSKAVNRLLN 140 Bombi...
KRN26673                57 HLKLYQTWFCKKRLCPLCNWRKSMKNSSQLKQIIAEAVA--RepkgrFLFLTLTIkNaYSAEELKGSLRTLTKAFNKLVK 134 Lacto...
CAL80011               182 FAPVKRAVAAYVRVIEFTRNAV----------------------------TQLWHPHAHWLLLVPKSYFAKgsKTFIDHD 233 Brady...
EFX41640               154 VKAWQKSILGYIRAIEYIGDKTp---------------------------VGECHLHYHSVLVVPQSYFRG--GNYMTQA 204 Helic...
EDX73127               128 SLRRSGVVVGYIKALEVTWSLSq---------------------------FGDTHPHYHVMFFLSGGRFPR---WLLDDM 177 Coleo...
jgi:Chro_5978          139 RKVFPAL--GWVKSFEVTRSKD-----------------------------GTAHPHFHVLMMVPPSYFK---KDYISQQ 184 Chroo...
Q1QGV9                 157 YKRVSAAVSAWYRTTEITRNPE----------------------------TGEFHPHIHLLVFFPPEYFKRakGLYITQA 208 Nitro...
PRJNA230994:X953_19940 123 RKEVSKITKGYVRKLEVTYDKErfitknmyrhrksyyelrglnigdnnpnYNTYNPHFHVLITVNKSYFTQt-KEYIKHE 201 Virgi...
WP_016252524           138 RAKVKKAVIGYLRAIEVTyna----------------------------erdDYHPHIHVLLMVKASYFKKt-EYYISQD 188 Enter...
WP_012655909           149 YKKISKNLIGFMRTTEVTvns----------------------------ndgSYNQHLHVLLCVEPSYFNSk-eNYIEQK 199 Macro...
WP_046316098           141 YKKVSQNLLGYVRSLEVTvndq-------------------------dleNITYHAHCHVLLFMKREYFSDs-QNYLEQS 194 Bombi...
KRN26673               135 YKKVHKNLLGYLRSTEITvne----------------------------qdgSYNQHLHVLLFVKSSYFTGdsVNYIKQE 186 Lacto...
CAL80011               234 RWSKLFRKALRCAYTPIVDVRVAKGVSSPLSeAGL-------------KPLREIAKYTTKPGTLvywdngmpyavgrqrp 300 Brady...
EFX41640               205 TWCDMWQKALRSDYKPIVDIRGIRPKAKAKLqPTSlktqanainpalvSALCEVVKYLVKPAKIakld------------ 272 Helic...
EDX73127               178 EWSRMWARSLQVDYQPVCKAKFCRSGDL--------------------KSILELVKYCVKPSDLssd------------- 224 Coleo...
jgi:Chro_5978          185 KWRELWQHALRADYEPVVDVRAIKPKSGSIDpADPskp--------llATIKETLKYTVKGQDLaad------------- 243 Chroo...
Q1QGV9                 209 EWAGMFKKALQVDYTPVCDIRAMPGVGGGAPlDDLg-----------rKSLFEVCKYVCEPGMFfeged----------- 266 Nitro...
PRJNA230994:X953_19940 202 RWLELWKQVTDNPNITQVDVRKVRE---NKN-----------------KEVSEIVKYTAKDSDYlid------------- 248 Virgi...
WP_016252524           189 EWTTMWQQSAKLDYKPIVDVRKVKPNQRKAKnEMdl-----------rGAIVETAKYPTKPIEkmgkte----------- 246 Enter...
WP_012655909           200 EWIKFWKRALKINYEPVVHVKAITPNRKKD---Sai-----------vSAIKETSKYSVKESDyltdde----------- 254 Macro...
WP_046316098           195 EWTNLWRRALKVDYQPIVHIQAVKKNSKRQn-KNsl-----------lSAAQETAKYQVKSADiftgfa----------- 251 Bombi...
KRN26673               187 EWAKLWQRALKSDYEPVVHVQAVKANKRKG--TDsl-----------qASAEETAKYEVKSADymtadd----------- 242 Lacto...
CAL80011               301 elyrkakgaplepmfnvpVMALADAVKGRRLVALSRNLETDeELDFTDDPRgDELDQTtedlga-------ficteFYEW 373 Brady...
EFX41640               273 ---------------tdqFETLDTQAKGLRQYNLGGLLKEY-DPLPPDELDpTLWELLeq---------------eIYKW 321 Helic...
EDX73127               225 ---------------pdwLYDLTDQLYKKRSLTVGGIVSQYvKQSMLDRI-----DCVgrsgdeq----nqdglpaSLDW 280 Coleo...
jgi:Chro_5978          244 ---------------aswLVELTHQLHKTRAIALGGVLKNY---LSEEEP--DQGDLIhgdedqgd--vspddprwWFSW 301 Chroo...
Q1QGV9                 267 -------------legfpLLELHEALHGRRLIGMSANLQRImDEQSLPQEA--PDDFIpvngrlpe--gavlegreIYHW 329 Nitro...
PRJNA230994:X953_19940 249 ---------------esvFEVFYKALKGKRIIVHSGLFKESmKLFKNGELD-Kykeidt------------teyvyALMY 300 Virgi...
WP_016252524           247 ------------kqklkvTDDLMKALKNKRQIGFGGLFKEIrKELQLEDVE-NG-DLVhiddenee---sslgveiVAQW 309 Enter...
WP_012655909           255 ------------eknleiISDLEEGLYKKRMIAYGGLLKIKhKELNLDDAE-DG-NLIqtsdeekinseeekahsiIAIW 320 Macro...
WP_046316098           252 ------------dldlevIDVLEKALSGTRQISFGGLFKKLrHDLQLDDAV-GAFNLNltaedlnyleelyvphkiVGAI 318 Bombi...
KRN26673               243 ------------ernltvIKDLEYALAGTRQISYGGLFKQIkQDLKLEDVE-NG-DLVhvgdedytkeqmeaaeevVAKW 308 Lacto...
CAL80011               374 Rgr----grHgdYF 383 Bradyrhizobium sp. ORS 278
EFX41640               322 S--------GqaYS 327 Helicobacter suis HS5
EDX73127               281 Dyi----gdR--YI 288 Coleofasciculus chthonoplastes PCC 7420
jgi:Chro_5978          302 Rem----ikR--YM 309 Chroococcidiopsis thermalis PCC 7203
Q1QGV9                 330 RqgatpaqsR--YV 341 Nitrobacter hamburgensis X14
PRJNA230994:X953_19940 301 Swg----naD--YV 308 Virgibacillus sp. SK37
WP_016252524           310 Nwe----rmT--YV 317 Enterococcus columbae
WP_012655909           321 Nfe----kqN--YF 328 Macrococcus caseolyticus
WP_046316098           319 Dhn----ppE--DM 326 Bombilactobacillus mellifer
KRN26673               309 Dfn----kqN--YF 316 Lactobacillus similis DSM 23365 = JCM 2765
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