2Z9O,2NRA


Conserved Protein Domain Family
Rep3_N
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pfam01051: Rep3_N (This model is not part of the current CDD release)
Initiator Replication protein, WH1
This protein is an initiator of plasmid replication. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This entry also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene. These proteins consist of two domains containing a winged helix-turn-helix motif, the N-terminal one, WH1, represented in this entry, is involved in the dimerization of RepE and an auxiliary binding to DNA. The C-terminal one, WH2, is related to a sequence-specific binding to the common 8 bp of the iteron.
Links
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Statistics
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PSSM-Id: 513958
Aligned: 155 rows
Threshold Bit Score: 44.5295
Created: 13-Feb-2025
Updated: 28-Apr-2025
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
2Z9O_A        33 IVQSNDLTeaAYSLSRDQ---------KRM-----------LYLFVDQI--RKSdgt---lqEHdgicEI--HVAKYAei 85  Escherichia col...
6_pfamImport   2 YKKNHNLIysRISMTTHE---------QNL-----------LALFLIQM--QktewkeetqlEI----SI--PASRLAdw 53 
WP_011998865  19 IKKGHQLVfsRQDLSARE---------ADM-----------FALMIAHM--Kandwe-rstpHY----EF--TSHQLSew 69  Vibrio campbellii
Q9LAL2        19 IFKSNALVegAFDLSPAE---------HDL-----------MTLAINKLfs----------qGVgg-kQVfiSAKEFAia 67  Moraxella catar...
Q8E8A8        57 QDLSNDLV--NLDVCQKEgydlvtvrgQRLnietdfkvwcgIVLVFSKYgySSN--------TV----KL--TFSEFAkf 120 Shewanella onei...
Q71TA8        54 IDATKAFS--QLEIAKAEgyddikitgPRLdmdtdfktwigVIYAFSKYglSSN--------TI----QL--SFQEFAka 117 Escherichia vir...
Q6U5J6        62 TDATEELV--QLSIAKSEgytdvkitgSRLdmdtdfkvwlgIIRSMSEYgvKSD--------TL----EL--SFVEFVkm 125 Klebsiella pneu...
WP_000361621  48 TDATEELT--RLSLARAEgfdkveitgPRLdmdndfktwvgVIHSFARHkvIGD--------KV----EL--PFVEFAkl 111 Escherichia coli
O68759        65 MDASAELK--QLSLAKAEgyedirisgVRLdmdndfktwvgIIHAFAKHkvVGD--------TV----TL--PFVEFVrl 128 Yersinia pestis...
1_pfamImport   2 IDASSELQ--SLELARAEgytdiritgPRLsmetdfkvwvgIILAFSKYglNSS--------TI----EL--PFSEFAtf 65 

2Z9O_A        86 fGLTSAe----ASKDIRQALKSFAGKE-V-VFyrpeeDa-------------------GDEKgye----sfPWFiKRAHS 136 Escherichia col...
6_pfamImport  54 lEVESks----lAATIKPVALRLQKRTvLaEHp------------------------tTHefe------ssVLFpTVKYA 99 
WP_011998865  70 lGVESkh----iGSNLSPVANRLASRKiGiKIe------------------------tGKGDief---dyrPLFkHIAYK 118 Vibrio campbellii
Q9LAL2        68 nQINEny----aYEVLAETAKRLQKRT-F-KA-----KiykdglkklvgeedpysvarPKGDyelld-lhanwVqAVAYQ 135 Moraxella catar...
Q8E8A8       121 cSYPSRrfdknLRKQIGDSLGRIQSQS-L-SF-----R--------------------RKNSeka---vhtGMLlRAMYD 170 Shewanella onei...
Q71TA8       118 cGFPSKrldakLRLTIHESLGRLRNKG-I-AF-----K--------------------RGKDakgg--yqtGLLkVGRFD 168 Escherichia vir...
Q6U5J6       126 cGFDSRrsnkkMRDRISNSLFKLASVT-L-QF-----Q--------------------SETkgw-----ttHLVqSAYYD 173 Klebsiella pneu...
WP_000361621 112 cGIPSSqssrkLRERISPSLKRIAGTV-I-SF--------------------------SRTTekhtkeyitHLVqSAYYD 163 Escherichia coli
O68759       129 cGIPTArssakLRKRLDSSLTRIATNT-I-SF-----R--------------------SKGSdey---yvtHLVqTAKYS 178 Yersinia pestis...
1_pfamImport  66 cGFSSKdkdkgLRTRLADSLIRLRSTT-I-KL-----A--------------------SEKDrng---vvsGLLsRGKWD 115

2Z9O_A       137 PSRGLYSVHinpyLIPFFIGLQN-R-FTQFRLSETKEI-TNPYAMRLYESLC 185 Escherichia coli K-12
6_pfamImport 100 R--GKLTMVpnydLRDFFHDASKg--YASVNVARFLRL-RGKYSKPMYDLLS 146
WP_011998865 119 N--GTLTMVpndmLKSEYIEYNQg--FALINTRNFFDV-KKEYSKRLYELLS 165 Vibrio campbellii
Q9LAL2       136 EKSGFIYLMfs-dVLAYLIQKTG-EaYTKYPYEKTIDL-KRFHTKRLYELCC 184 Moraxella catarrhalis
Q8E8A8       171 GEEDIVELM----ADETLWDLYRlDyQVLVSLRVLEKLpRAEVAQCLYLYFT 218 Shewanella oneidensis
Q71TA8       169 ADLDLIELE----ADSKLWELFQlDyRVLLQHHALRALpKKEAAQAIYTFIE 216 Escherichia virus P1
Q6U5J6       174 INEDIVEIK----AEPKLFELYHmDrRVLLRLKAIDALqRKESAQALYTYIE 221 Klebsiella pneumoniae
WP_000361621 164 TEKDIVQLQ----ADPRLFELYQfDrKVLLQLKAINALkRRESAQALYTFIE 211 Escherichia coli
O68759       179 TKNDTVSLQ----ADPKIFELYQfDkKVLLQLRAINELsRKESAQALYTFIE 226 Yersinia pestis KIM10+
1_pfamImport 116 EKDDIMELT----ADESLWELYQfDrQVLLQMFIIRQLaNKGTAQALYTFIE 163
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