1SRA


Conserved Protein Domain Family
SPARC_Ca_bdg

?
pfam10591: SPARC_Ca_bdg 
Click on image for an interactive view with Cn3D
Secreted protein acidic and rich in cysteine Ca binding region
The SPARC_Ca_bdg domain of Secreted Protein Acidic and Rich in Cysteine is responsible for the anti-spreading activity of human urothelial cells. It is rich in alpha-helices. This extracellular calcium-binding domain contains two EF-hands that each coordinates one Ca2+ ion, forming a helix-loop-helix structure that not only drives the conformation of the protein but is also necessary for biological activity. The anti-spreading activity was dependent on the coordination of Ca2+ by a Glu residue at the Z position of EF-hand 2.
Statistics
?
PSSM-Id: 313749
View PSSM: pfam10591
Aligned: 22 rows
Threshold Bit Score: 113.95
Threshold Setting Gi: 74800039
Created: 26-Jul-2016
Updated: 4-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1SRA_A         1 PPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNnlltekqklrvkk-ihenekrleagdHPVELLARDFEKNYNMYIFPV 79
gi 122121327 166 RICDGEDLKEFPRRMRDWLFNVMRDLAERDELTe-------------------------HYMQMELEAETNNSRRWSNAA 220
gi 122107838 182 PDCTESEMSDFPRRMRDWLFNIMRDMAERRELTp-------------------------HYLKMEREAESNLTRRWANAA 236
gi 122067132 178 PDCSENDLADFPRRMRDWLFNVMRDLAVRNELPe-------------------------AYMELEQEAESNLTKRWTNAA 232
gi 808358124  52 DECTEEHMAQFPERMADWLFQVMKELKKRRELHk------------------------lEWEELLSEAENDDEKKHVYPV 107
gi 74096141  214 QPCGEHELSEYPTRMRSWIKNIYLQMYDEAEDMgglnekqrfhgrklvanrdrlqtyeeHHIDLMSREFQKFYPLYKYPI 293
gi 148539548 149 gSCSAEDLREYPERMTNWFIKSLALIRNRPEEHg-------------------------GLSQKEKETLQQEWSQSTHAI 203
gi 156373068 111 SECARDNLLQFGERYLEWILISRRRLVNRYYQ-----------------------------VKESEKGHNLPPDMRKELA 161
gi 156364615 110 TDCPKWMLEQFPYRFLDWLYLTRELSRDPRFEI-------------------------------AKRGEQLTGDEIKEVL 158
gi 326918678 524 PHCTDYEVGQFPLRMRDWLKNILMQYYERDQDKpgfltekqrnkvkkiylnekrlvsgeHPVELLLHDFEKNYHMYVYPV 603
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
1SRA_A        80 HWQFGQLDQHpIDGYLSHTELAPLRAplIPMEHCTTRFFETCDLDNDKYIALDEWA 135
gi 122121327 221 VWKWCDLDGD-TDRSVSRHELFPIRAplVSLEHCIAPFLESCDSNNDHRITLIEWG 275
gi 122107838 237 IWKWCDLDAQtNDRFVSRHELFPIRAplMALEHCIAPFLDRCDADDDHRVTLAEWG 292
gi 122067132 233 IWKWCDLDGHpNDNSVSRHELFPIRAplMTLEHCIAPFLETCDPNGDHRITLQEWG 288
gi 808358124 108 IWKFCELDTKpHDKSVSHHELIPITApvIPMESCIKPFLEGCDANNDGNISIKEWG 163
gi 74096141  294 HWKFGILDVNpTDKYLSKRELEPMRAplVPLEHCTDTFFSTADANKDHLISLYEWA 349
gi 148539548 204 FWKFDRLDKNpSDHYLDFAELEGLRApiVLFEPCTKPFLQACDVDNDTLISAVEFG 259
gi 156373068 162 QWEFDRVDFN-RDGVLSGREINSIIGplLFHERCIYGFVMSCDVNKNSVIDKQEWl 216
gi 156364615 159 AWEFNNWDLN-KNGIWDKEELDLVQTilMPIEGCSFGFLKSCDLDGKGDMDRREWM 213
gi 326918678 604 HWQFYQLDQHpIDRSLTHSELAPLRAslVPMEHCITRFFQECDGDQDKLITLKEWC 659
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap