Conserved Protein Domain Family
Atg14

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pfam10186: Atg14 
Vacuolar sorting 38 and autophagy-related subunit 14
The Atg14 or Apg14 proteins are hydrophilic proteins with a predicted molecular mass of 40.5 kDa, and have a coiled-coil motif at the N-terminus region. Yeast cells with mutant Atg14 are defective not only in autophagy but also in sorting of carboxypeptidase Y (CPY), a vacuolar-soluble hydrolase, to the vacuole. Subcellular fractionation indicate that Apg14p and Apg6p are peripherally associated with a membrane structure(s). Apg14p was co-immunoprecipitated with Apg6p, suggesting that they form a stable protein complex. These results imply that Apg6/Vps30p has two distinct functions: in the autophagic process and in the vacuolar protein sorting pathway. Apg14p may be a component specifically required for the function of Apg6/Vps30p through the autophagic pathway. There are 17 auto-phagosomal component proteins which are categorized into six functional units, one of which is the AS-PI3K complex (Vps30/Atg6 and Atg14). The AS-PI3K complex and the Atg2-Atg18 complex are essential for nucleation, and the specific function of the AS-PI3K apparently is to produce phosphatidylinositol 3-phosphate (PtdIns(3)P) at the pre-autophagosomal structure (PAS). The localization of this complex at the PAS is controlled by Atg14. Autophagy mediates the cellular response to nutrient deprivation, protein aggregation, and pathogen invasion in humans, and malfunction of autophagy has been implicated in multiple human diseases including cancer. This effect seems to be mediated through direct interaction of the human Atg14 with Beclin 1 in the human phosphatidylinositol 3-kinase class III complex.
Statistics
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PSSM-Id: 370867
View PSSM: pfam10186
Aligned: 28 rows
Threshold Bit Score: 136.032
Threshold Setting Gi: 74582122
Created: 2-May-2019
Updated: 18-Jul-2019
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q4WCL3     3 CPICSRVSssrLRFYCPTC---ACN--------------QIYTLRIDNARALLEKETLGRQVEKALLHQTsptlsyhrfe 65  Aspergillus fumigatus
CAR27445   3 CSICHKSG---RPMYCAHC---MSSsp-----------hMLLKLKLDLLILRETNGHLKSRVEDILEYGLgqi----agk 61  Zygosaccharomyces r...
Q6FX35     5 CPICETQS---HVFYCAHC---INSsp-----------dLLIRLKFDLYILGKINNALRNKVDQYLEEVDdeln--tnir 65  [Candida] glabrata ...
Q5KCM1     9 CNCCQVNQ---KALYCPAC---LRE--------------GITLHKELLQNLQARLSTIRQHSQKLLTGTE---------- 58  Cryptococcus neofor...
EDR01346   3 CKICEFKQ---RQFFCQTC---LKT--------------HLRDFQHQTQHFAADRDEQVLKASKALETIQ---------- 52  Laccaria bicolor S2...
Q17M47    35 CPLCNYSK---RHFHCKSC---IRKgdfihs--thhileRFSEKQLRHMNLKSAHATLEGKCVKLLERRR---------- 96  yellow fever mosquito
Q6ZNE5    43 CPLCNTTR---RRLTCAKC---VQSgdfvyf--dgrdreRFIDKKERLSRLKSKQEEFQKEVLKAMEGKW---------- 104 human
EDO36555   3 CPLCLRRS---KYLTCCSC---LRSgsvtht---gkrpeSYCEKVKRLADLKHVKEAFQKRVTEEIANRQ---------- 63  starlet sea anemone
Q54QH5     7 CCSCNNRFn-fQESYCEPClqrIKNdq-----------sRVFELIQSIFDKKKANLIAKNKILESISQPK---------- 64  Dictyostelium disco...
O42862     7 CQLCETR----RPSICESC---LKKqlydfyhkrdefsrDIESELEKVAKLKSESFSLKGKITQKEELTY---------- 69  Schizosaccharomyces...
Q4WCL3    66 ECSTQSPDKVPSPQDVLQIITAKAESSIR----------RKELALQIQQLKSEIKDRQLGISQRRLTLA----------- 124 Aspergillus fumigatus
CAR27445  62 EPKSASTNVEGDILGRRLRKLDSVILRRK----------NNRVKYRISQLNRRIYEKRHRIEALRSKL------------ 119 Zygosaccharomyces r...
Q6FX35    66 NGEQLESNIGVQILKERLGKVQVLRKERQ----------NNKIKHKISQQDRRIKEKSRYIAELRSLI------------ 123 [Candida] glabrata ...
Q5KCM1    59 --NETKDKADRGLNAWRELRSDVASSEAR----------CHQIKMTISQRQAAISACRKRIAENNIR------------- 113 Cryptococcus neofor...
EDR01346  53 --------------AARLFRAHVADCQSR----------LEEVMAGLAQLRKENDKKRDRLRALRESLA----------- 97  Laccaria bicolor S2...
Q17M47    97 LSCQLRAEIKQRTENIDNIRKLMALKREN----------VGRLQALRKELYESNRLLRMKLPRCDDQVKrissyvlskle 166 yellow fever mosquito
Q6ZNE5   105 ITDQLRWKIMSCKMRIEQLKQTICKGNEEmeknsegllkTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGdl--------- 175 human
EDO36555  64 AVQQLNDAVILKREKISCIQSVIRLIQQH----------TSQDQQSLDSIRKSNAVKKVKLKSNADEIKtllevkkkhla 133 starlet sea anemone
Q54QH5    65 --NQLQEQIDIKKTYLESKKIQIEILKNK----------IQTLKTTVIKDNGLIDVKKKYLSTRRSSLK----------- 121 Dictyostelium disco...
O42862    70 -------RLQNLAASLNEKKSLSCDLHSR----------KQLIEDDLSNRKKSINIASQKLAGLSHSTSdyfsk----ey 128 Schizosaccharomyces...
Q4WCL3   125 ---RRRSDAESSKFQLESREVALICGVQNTIK----------------RTERL------------WHSLHSKTVEA---- 169 Aspergillus fumigatus
CAR27445 120 ---QLPSKIGIHEKELKAKRESQELTIS--------------------EGKQQ------------LTQIRKVLLKS---- 160 Zygosaccharomyces r...
Q6FX35   124 -----------NGPPKNQNRFTDPQTILKAQK----------------QLQDK------------LEIAKRLSIQT---- 160 [Candida] glabrata ...
Q5KCM1   114 ---DRRES-----LSMLKSRPSPVSSLRDSIK----------------QVHSQ------------QQVITSRIIHA---- 153 Cryptococcus neofor...
EDR01346  98 ---TRRRS--LSAAKLQPARHSPAPGLSHLTQ----------------RETQE------------LNALSMTIARA---- 140 Laccaria bicolor S2...
Q17M47   167 eseRRRASINELQDEIKQFRRDQIGKLIRYIF----------------PISRA------------ISNRSGFSSS----- 213 yellow fever mosquito
Q6ZNE5   176 -veKKTIDLRSHYERLANLRRSHILELTSVIF----------------PIEEV------------KTGVRDPADVSse-s 225 human
EDO36555 134 tlsKRMRDLKSLQNELGQVRQRSMALVQKYLF----------------PIQAIpvypvmgtppppQHTPESLDLSRelsf 197 starlet sea anemone
Q54QH5   122 --------KNKISFEAVNNSKSGFSSLTDEIQ----------------KKKDQ------------LTAENTSLQIV---- 161 Dictyostelium disco...
O42862   129 ltaYRRSE--LLQNKLHEYQKKLITRVLDMYQlhwqrdlvlntqgctqKSAQM------------GSEFNPDSIDSsl-a 193 Schizosaccharomyces...
Q4WCL3   170 -RIFLCREVANLYSLRQ----------WMKQDGSEMKETYVIGG--VPIVDLRDLNGksqncpllldm--------paps 228 Aspergillus fumigatus
CAR27445 161 -QLSKIRSLVEWFVIRK---------------RESYEFPYTLAF--QPIVSLRNVYK----------------------- 199 Zygosaccharomyces r...
Q6FX35   161 -RSEKLAMLNKWYSIRK---------------RDSHDIPFTISY--QPVISLKNFNR----------------------- 199 [Candida] glabrata ...
Q5KCM1   154 -RRVLVREAVNVFGVRQ-----------------RPSGDWEIAG--IPLPDPETFRV----------------------- 190 Cryptococcus neofor...
EDR01346 141 -RSGLVQELVEVFNVVEvg------grPPIGGKAGTKGEWTIGD--LILPVPGDIRR----------------------- 188 Laccaria bicolor S2...
Q17M47   214 -DGESKHTISEISDATRtayv---rgkWVLQDS-FGEIQHIIVA--PALPGNGNYSAyndwatvnpatdvsstgrsedgv 286 yellow fever mosquito
Q6ZNE5   226 dSAMTSSTVSKLAEARRttyl---sgrWVCDDH-NGDTSISITGpwISLPNNGDYSAyyswveekkt----------tqg 291 human
EDO36555 198 eDQADASVESALAEATQtsyv---qgkWV---NETATLELSIID--SSLPWSGDYSAyrhwvkahkng-------skgpd 262 starlet sea anemone
Q54QH5   162 -KRSKIEQLTNSMTRLQ--------------LNPTTPDHFFLFN--MVLNDQQLLK------------------------ 200 Dictyostelium disco...
O42862   194 yRLSKLSMGNSKSDLSHsdnetghfysNFFSQVMELNASVTISG--IPVCIRSKEKM----------------------- 248 Schizosaccharomyces...
Q4WCL3   229 inaqigATAVQISTSFSNIAHLLVLVSHYLSLRLPAEITLPHKDHPVPTIHAPIASYISRESILTAtstq-phnLASSTT 307 Aspergillus fumigatus
CAR27445 200 ------LPSSVVWGSISTMSRYLKLFAEILFFTLPHNELVAPNAP----------------------------------- 238 Zygosaccharomyces r...
Q6FX35   200 ------LPLPLIEGSLRKLIQYMNLLEHIYCIKYPSAMFVHEDEM----------------------------------- 238 [Candida] glabrata ...
Q5KCM1   191 ------HPSSSINAALSHVIHLVSLITTYLSISLPFVPMPPPPFYHPHVGRPLMKANLPFVSTTKWrekhvlwmSSTASV 264 Cryptococcus neofor...
EDR01346 189 ------YPPDHINAVITHTIHFLSLLTFYLGVKLPFEFVWTGGKLGVGQPWIGAIMGSESGGWARWstk--hplHLSSSP 260 Laccaria bicolor S2...
Q17M47   287 rnvasrNPAHTIAAALTYTAQLVHIVCFYLDIRAPYKVSYSDFCT----------------------------------- 331 yellow fever mosquito
Q6ZNE5   292 pdmeqsNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCG----------------------------------- 336 human
EDO36555 263 ddvglrNPAYTISSALTHAAQLLELLASLLDVNLPSKLSYSEFCQ----------------------------------- 307 starlet sea anemone
Q54QH5   201 ------FPKDVIFTTLGYISKIVVLISGILGITLPYHLLSKGSKSTI--------------------------------- 241 Dictyostelium disco...
O42862   249 ------FLNPDCALTLSFICIFLA---QYTSIPLPCPLQLPSPDQ----------------------------------- 284 Schizosaccharomyces...
Q4WCL3   308 HPLGFQSNHYQPRPLTIDRSLPKLAR--EDPESYVLFLEGVTLLAWNVSWLCRTQGLNI--------ASDSWEEI----- 372 Aspergillus fumigatus
CAR27445 239 ----------------ELELEVSAKTadESSSDENTIVDHLTKIFINIIQISRHLNLISkd----piDLAWMLDQ----- 293 Zygosaccharomyces r...
Q6FX35   239 --------------------LSLQGHsaKLKRNAPELLQVLIKLIQMILVVMTRSKLIDerk--qniDYAWILDQ----- 291 [Candida] glabrata ...
Q5KCM1   265 SSKIKPQSTPPISNLLAQPSICSIIA--KSTSKHKQFLVSFALLSFSIAYLAWSQDVPGigirgevdGDDNAGVL----- 337 Cryptococcus neofor...
EDR01346 261 NPPQPDETPPTPTAPLSESYIDTTP----PPNTPSSFTTALAMLLYDVSYLAHTQNVDV-----------PLSQA----- 320 Laccaria bicolor S2...
Q17M47   332 --------------T---------------ALSEAQFNRKVARLNANILYLCYTQGCQLnel----rptHTLENI----- 373 yellow fever mosquito
Q6ZNE5   337 --------------E---------------NLSKQKFTRAVKKLNANILYLCFSQHVNLdql----qplHTLRNL----- 378 human
EDO36555 308 --------------N---------------EMGRKEFQSALSRLNTNVLHLCFTQHVDPtll----hphRTLNNL----- 349 starlet sea anemone
Q54QH5   242 ------------HHNMKSKDYPLYYQ---QKTKSREFQKALHLLGENIIFLRYYHGIQTp-------KESNLLFL----- 294 Dictyostelium disco...
O42862   285 ------------------------------KPMTSFSSEQMLYIMYNIVWISWNCGIFSfprgitqsQLFELMQYtgfwl 334 Schizosaccharomyces...
Q4WCL3   373 ----------------------CNIGKNMW----------------QLLVA 385 Aspergillus fumigatus
CAR27445 294 ----------------------NDLDTLFY----------------NMAMR 306 Zygosaccharomyces rouxii
Q6FX35   292 ----------------------YDIDGLFY----------------HMAMS 304 [Candida] glabrata CBS 138
Q5KCM1   338 ----------------------FDEES-------------------KVTQG 347 Cryptococcus neoformans
EDR01346 321 ----------------------GDVLSNLW----------------SVCCS 333 Laccaria bicolor S238N-H82
Q17M47   374 -------------------------QRLMN----------------STHAD 383 yellow fever mosquito
Q6ZNE5   379 -------------------------MYLVS----------------PSSEH 388 human
EDO36555 350 -------------------------YALIN----------------TTCTG 359 starlet sea anemone
Q54QH5   295 --------------------------KNIY----------------ELIKS 303 Dictyostelium discoideum AX4
O42862   335 vqlrtkpltsqkpdwhykmgmdFDLFHRLYlarlpipinysslrsrSSSKP 385 Schizosaccharomyces pombe 972h-
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