1SJ7


Conserved Protein Domain Family
Talin_middle

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pfam09141: Talin_middle 
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Talin, middle domain
Members of this family adopt a structure consisting of five alpha helices that fold into a bundle. They contain a Vinculin binding site (VBS) composed of a hydrophobic surface spanning five turns of helix four. Activation of the VBS causes subsequent recruitment of Vinculin, which enables maturation of small integrin/talin complexes into more stable adhesions. Formation of the complex between VBS and Vinculin requires prior unfolding of this middle domain: once released from the talin hydrophobic core, the VBS helix is then available to induce the 'bundle conversion' conformational change within the vinculin head domain thereby displacing the intramolecular interaction with the vinculin tail, allowing vinculin to bind actin.
Statistics
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PSSM-Id: 337308
View PSSM: pfam09141
Aligned: 26 rows
Threshold Bit Score: 122.745
Threshold Setting Gi: 167518654
Created: 27-Mar-2017
Updated: 23-May-2017
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1SJ7_A          10 AQQALTGTINSSMQAVQAAQATLDDFETL--PP---L--GQDAASKAWRKNKMDESKHEIHSQVDAITAGTASVVNl--t 80
gi 75020100    525 PQRALIGTIEATIRAVEDAEIELEAEPQIdiPR---F--NDDYSQNRWMEEQQAVNKENVNERLAAMGAATAQVVQwtav 599
gi 674591712   430 PKRAVMLRIDESTKEIQDASSSLQKPYFE--DD---SvsRDDSATQRWREENMAQARAGICSYLGAMTIATGNLISl--l 502
gi 674579882   505 PKRAVMHRIDESTKEIQDASDSLQKPYFE--DNdtvS--RDDSATQRWREENMAQARAGICSYLGAMTIATGNLVSllql 580
gi 358333811   437 PKRALRSRVDLGLRTIKEAAEELDTPIYA--DDdaiR--GDDATTKRWRVEALEQARNGVWSHLGAMTMATGQLISc--l 510
gi 1084294059  519 PKRALLSRIDYGVRTIQGACEDIDKPIYE--DEealY--GDDINTKRWRSETLQQARAGVLSHLGAMTMATGRLISgiei 594
gi 514699215   459 AQSTLANHISRANRVFVLAGKKIASPATL--PP---L--GESASAHRWREEKDLLARQNVAAEVAAILSATANIVTl--t 529
gi 195996555   470 pKAAFLSNISAGFMAASATTAALDEPAEL--PD---L--GSDPASLQWKRNTLHISQENVRANIAAINGSAAAIILl--t 540
gi 156402925   505 AQQAYMSSLSQGIDSVDTADEELKRPVNI--VA---L--GSDPASLKWKQNTLDVSRQNVASRLAALSASCASIVNv--t 575
gi 1133454380  495 AQQNVATVCGRSLQEVMAITEQLQTPLEV--PV---M--NNDSVSMKWRQKTLDVSRQNVSAALAAMLASAASVITq--t 565
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1SJ7_A          81 A----------------GDPA---------Etdy---------------taVGCAVTTISSNLTEMSRGVKLLAAL---- 116
gi 75020100    600 E----------------EYDD---------R--------------------VGTAIATIGSNLPDVSRNVRDLGAF---- 630
gi 674591712   503 QpsqgrmdtdgsqesnyEDDD---------Avdy---------------taMDASLATIGLNVQRLMQNVRVLDQL---- 554
gi 674579882   581 S----------------SHNR---------VgdssqegmeeeeedaidytaMDASLATIGLNVQRLMQNVRVLDQL---- 631
gi 358333811   511 Qttaqt-------ppsgGPNV---------Efdy---------------tnMDASLASIGMNVHGLMQCVRLYDQIdlan 559
gi 1084294059  595 S----------------NNRRrngdmnnhaEidy---------------asMDASMTSIGMNVKGLMDCVRLYNQL---- 639
gi 514699215   530 Ta--------------rSDNT---------Kvnf---------------taVGASISTLASNMRSAAEDVAMLMAL---- 567
gi 195996555   541 S----------------NPDK---------Eidy---------------taIGSSVTTISSNMNDMAKRIKMIAAL---- 576
gi 156402925   576 S----------------GEPG---------Dtny---------------taAGAAVHVISANLQDLSKGIKLIAGL---- 611
gi 1133454380  566 S----------------LEPE---------Einy---------------taVGSSVTTISTNLSELAKAVRMLAAL---- 601
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1SJ7_A         117 -L----E---DEgg--ngRP--LLQAAKGLAGAVSELLRSAQ--------P--------A-------------------- 148
gi 75020100    631 -M----E---HRe----rGD--LLDATKKLCGAFGKFLNTVN--------P--------E-------------------- 660
gi 674591712   555 -Q----AvdgNDes---qGY--IMSAAQEVADAFLKLMKSAMplkegvqkP--------G-------------------- 596
gi 674579882   632 -Q----A---VDagdesqGH--ITNAAQDVADAFLKLMQSAM--------PlkegkqgsG-------------------- 673
gi 358333811   560 sD----G---VDeh---vSG--LKEAAQALSDAFLELMRATV--------P--------Tarsegdldkasslslssnsl 611
gi 1084294059  640 -DtvnnD---NGsv---vDKtsLKQAAQALSDAFLDLMRTTF--------S--------Svsldnssdkast-ltrstss 695
gi 514699215   568 -N----P---VQa-----DN--FKSALEQLSSHLRSFVDTVA--------P--------T-------------------- 596
gi 195996555   577 -Q----D---DEy----sVD--LITATKELASAFVKLLKSSQ--------P--------E-------------------- 606
gi 156402925   612 -L----D---NQdd---sSK--LLDAARGLAGAFSLLLKAAQ--------E--------G-------------------- 642
gi 1133454380  602 -N----P---SEge---gSN--LLDAARALAAATADLLNSAH--------P--------Q-------------------- 632
                          250       260       270
                   ....*....|....*....|....*....|.
1SJ7_A         149 -----S---AEPRQNLLQAAGNVGQASGELL 171
gi 75020100    661 -----T---EARRNEVFRTAGHVGETSQHLI 683
gi 674591712   597 -----S---ATDRPNLYEAASKVGDTSQQLL 619
gi 674579882   674 -----T---VVDRPSLYEAASRVGDTSQQLL 696
gi 358333811   612 aeqsrS---GPDRAALLEAASRVGDASRQLL 639
gi 1084294059  696 lfdskS---STDRAALLEAASRVGDASRQLL 723
gi 514699215   597 -----Er-gIMNRQNILSSANQVGQSGSALL 621
gi 195996555   607 -----S---MESRQSLLEAANDAGIHSKRIL 629
gi 156402925   643 -----AggdKEKRSQLLNAAGNVGSSGAQLL 668
gi 1133454380  633 -----N---LHKRQELLASAGDMGTTGGQLL 655
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