Conserved Protein Domain Family
SAE2

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pfam08573: SAE2 
DNA repair protein endonuclease SAE2/CtIP C-terminus
SAE2 is a protein involved in repairing meiotic and mitotic double-strand breaks in DNA. It has been shown to negatively regulate DNA damage checkpoint signalling. SAE2 is homologous to the CtIP proteins in mammals and an homologous protein in plants. Crucial sequence motifs that are highly conserved are the CxxC and the RHR motifs in this C-terminal part of the protein. It is now known to be an endonuclease. In budding yeast, genetic evidence suggests that the SAE2 protein is essential for the processing of hairpin DNA intermediates and meiotic double-strand breaks by Mre11/Rad50 complexes. SAE2 binds DNA and exhibits endonuclease activity on single-stranded DNA independently of Mre11/Rad50 complexes, but hairpin DNA structures are cleaved cooperatively in the presence of Mre11/Rad50 or Mre11/Rad50/Xrs2. Hairpin structures are not processed at the tip by SAE2 but rather at single-stranded DNA regions adjacent to the hairpin. The catalytic activities of SAE2 are important for its biological functions.
Statistics
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PSSM-Id: 337115
View PSSM: pfam08573
Aligned: 70 rows
Threshold Bit Score: 48.9288
Threshold Setting Gi: 74879020
Created: 27-Mar-2017
Updated: 23-May-2017
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 121781944   567 AFDEVVRSREERA-ELSG--CTDPNCCGRQFRAMAESElsa---------------------gglGILSRVADIKMMEDY 622
gi 74863551    825 plnKKVHMNNSKK-SIYYshSLSPTCl--------------------------------------NAHTDTEKASMNEKK 865
gi 74879020    655 TFYEICDDNQSKKnEI---------------------------------------------------------------- 670
gi 667789572   512 AYDEVVRDKASRR-HLPA--CACDN-CIKFFEAHGPIAtif---------------------kpkWRSPLKK-------- 558
gi 353234934   511 AYDEVVRNKAERK-RMEA---TDCDQCREFYKAAGPLPprlqap----------------lwkspTSSQDPAAAPQRHCK 570
gi 221054167   538 nekytngtqpdtvksfgemalvsspryfkngegnvdmgsgkyaekyidkfmkkfemkeknkgslvkmnqveeekfyevDE 617
gi 302840718  1212 KYSAVVRGRAERD-ALQAveCAG---CRAFYEAVATWGgsvepccctharggrtaaapdgapraqAAAAAAAAAAMQTAA 1287
gi 901891678   615 EFYEVEEADDAEEvee---------------------------------------------------------------- 630
gi 443926073   548 QFDSVVRDKNERK-KLAAgdCIA---CKEYYEAVGAMParpraplwkspp---pegagpslrphsCKHREGGADLSVSDD 620
gi 1069862728  512 AYGEVVRDKASRK-HLPAcaCDN---CIKFFEAHGPIAtifkp------------------------------------- 550
                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 121781944   623 LG---TE--AYQLVEMTGEERQETWLKA---KIQDLAN--RLGRHRHRFARRPSPPGYWNPDFP 676
gi 74863551    866 NGnmlNMdnI---------KKENDLEKEkekKKKKIIQ--TFSRHRYHNKINDSPKNFWKFDFF 918
gi 74879020    671 -------------------------------NKKKAIQ--AYSRHRFHNKVNDSPQNFWGFDFF 701
gi 667789572   559 -------------KTYTDSDKE---------HIYRNLQ--EVSRHRSAFFRPKTPPGFWESDFP 598
gi 353234934   571 HH---SN--KEESDD-SGSESEDSGVRA---HRQE------VSRHRYIMPSGETPPGFWDLDFP 619
gi 221054167   618 VD---DT--EEELPTEVAEDRRK---EN---KKKKLIQ--SFSRHRYHSKVNDSPKNFWSFDFF 668
gi 302840718  1288 -------------------QPQQPAAAAgapRALAAALrqAGGRHRFKFAPPATPDGFWDIGFg 1332
gi 901891678   631 -----------------aeEAEEAEARRkenKKKKLIQ--SFSRHRYHSKVNDSPKNFWSFDFF 675
gi 443926073   621 VD-----------------MEDDVGLAA---HKQ------AISRHRHKWVPASTPPDYWNIGFP 658
gi 1069862728  551 --------------qwrspSKKEVYTDSnkdHIYKNLQ--EVSRHRAAFFRPKTPPGFWESDFP 598
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