1X52,3MCA,3AGK,3OBW,3WXM,2QI2


Conserved Protein Domain Family
eRF1_3

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pfam03465: eRF1_3 
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eRF1 domain 3
The release factor eRF1 terminates protein biosynthesis by recognising stop codons at the A site of the ribosome and stimulating peptidyl-tRNA bond hydrolysis at the peptidyl transferase centre. The crystal structure of human eRF1 is known. The overall shape and dimensions of eRF1 resemble a tRNA molecule with domains 1, 2, and 3 of eRF1 corresponding to the anticodon loop, aminoacyl acceptor stem, and T stem of a tRNA molecule, respectively. The position of the essential GGQ motif at an exposed tip of domain 2 suggests that the Gln residue coordinates a water molecule to mediate the hydrolytic activity at the peptidyl transferase centre. A conserved groove on domain 1, 80 A from the GGQ motif, is proposed to form the codon recognition site. This family also includes other proteins for which the precise molecular function is unknown. Many of them are from Archaebacteria. These proteins may also be involved in translation termination but this awaits experimental verification.
Statistics
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PSSM-Id: 335338
View PSSM: pfam03465
Aligned: 40 rows
Threshold Bit Score: 98.7694
Threshold Setting Gi: 74513508
Created: 18-Mar-2017
Updated: 23-May-2017
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1X52_A        18 AAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVaTRSR---------------------- 75  human
Q97W96       275 YIEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEIGAIEAIVIHEDSTDKE----------------------------- 325 Sulfolobus solf...
Q9BMM3       280 FIREKKVMSKFFEEVAQDTKKYCYGVEDTMKSLLMGAVEVILLFENLNFTRYV-LKNPttgiektlyltpeqeenh---- 354 Blepharisma ame...
Q9BMX1       276 FVEEKNLISKYFEQIALDTGMIVFGVEDTLHSLEIGALDLLMCFENLEINRYE-IRDSandeikiynlnkeqekdpk--y 352 Euplotes aedicu...
Q9BMM1       285 FVAEKKLVSKFFEEISLDTGMIVFGVQDTMKALELGAVETILLFEELEITRYV-IKNPvkgdtrtlflnptqqkdsk--y 361 Stylonychia myt...
O16520       288 FIQEKKLIGGYFDEISQDTGKYVFGVKDTLAALEMGAIETLICWENLDIVRYK-MKNSlgedillnlrpdeekdksh--- 363 Caenorhabditis ...
P79063       277 YVQEKKLIQRFFDEISLDSGKYCFGVVDTMNALQEGAVETLLCFADLDMIRYE-FKNSegnpvitymtkeqeekdstnsf 355 Schizosaccharom...
Q9NAX8       281 LVQEKKLLQGFFDQIACDTQLYCFGVQDTLKCLEAGAVETLIVYEDLNIYRYTvVKNRgaddeetfvhvmseeeakrs-n 359 Trypanosoma bru...
XP_001908186 281 FIQEKKLIGKYFEEISQDTGRICYGVEDTLKALELGAVETLIVFENLEVTRWV-LKDSngaeiiihstkqqdaanrd--r 357 Podospora anser...
XP_001018735 279 FVQEKNVISKFFDCIAIDSGTVVYGVQDTMQLLLDGVIENILCFEELTTLRVT-RKNKvteqithifippnelnnp---- 353 Tetrahymena the...
1X52_A        76 ------------------YVRLVDSVKENAGTVRIFSSLHVSGEQL-SQLTGVAAILRFPV 117 human
Q97W96       326 ------------------LEKLAQDAENYGVKVFVVGDEVPEAEWVkKTFNGIVGKLRYRL 368 Sulfolobus solfataricus P2
Q9BMM3       355 dnfiengeelealekgplPEWIVDNYMKFGAGLEFITDRSQEGAQFvRGFGGLGAFLRYQV 415 Blepharisma americanum
Q9BMX1       353 fknektgadleiiksgplSEWLCENYSKFGIKLEFITDKSQEGFQFvNGFGGIGGFLRFKL 413 Euplotes aediculatus
Q9BMM1       362 fkdqasgldmdvisedqlAEWLCHNYQNYXAQVEFITDKSQEGYQFvKGFGGIGGFLRYKV 422 Stylonychia mytilus
O16520       364 ftdsetgqdmeiietmplLEWFANNYKNFGAALEIVTDKSQEGAQFvRGFGGIGGLLRYRV 424 Caenorhabditis elegans
P79063       356 lldkdtgaemelvssmllSEWLAEHYKDYGANLEFVSDRSQEGMQFvKGFGGIGAVMRYQL 416 Schizosaccharomyces pombe 972h-
Q9NAX8       360 ihmqesgktrneieqedfVDWLATNYRKFGCALELITNRSQEGTQFvRGFGGIGGVLRYKL 420 Trypanosoma brucei brucei
XP_001908186 358 fmdketgqemevvsqesfLEWIAEHYKDFGTTLEFVSDRSTEGNQFvKGFGGIGGILRYKV 418 Podospora anserina S mat+
XP_001018735 354 khfkdgehelekievenlTEWLAEHYSEFGAELYFITDKSAEGCQFvKGFSGIGGFLRYKV 414 Tetrahymena thermophila SB210
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