3QSV


Conserved Protein Domain Family
MH1

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pfam03165: MH1 
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MH1 domain
The MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localization signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx.
Statistics
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PSSM-Id: 308666
View PSSM: pfam03165
Aligned: 20 rows
Threshold Bit Score: 83.9629
Threshold Setting Gi: 14194972
Created: 17-Jul-2016
Updated: 4-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3QSV_A       28 AKRAIESLVKKLKEKk--DELDSLITAITTNgAHP------------------SKCVTIQ-------------------R 68
gi 74965558  36 WALKVTRLLSRVAKKh--QCFEAFYDAVI-KgDPk------------------TRCCPAHn------------------E 76
gi 74763404 217 FREDFEALMKQLKRK----QRNELLLAVKSRlDPPtktqrdvvepttttaptyLQCILIPc------------------- 273
gi 82177797  39 LRAAASAILKRLKEQt----LCVLLEAVESRgAAP------------------GGCVMVT-------------------R 77
gi 82121089  59 LKALAHCVLKKLKEKq----LEGLLQAVECKgGAR------------------SPCLLLPa------------------A 98
gi 13959538  92 LKALTHSVLKKLKER----QLELLLQAVESRgGTR------------------TACLLLP-------------------G 130
gi 13959573 106 LKAVTYALLKRLKERs----LHSLLQAVESRgGTP------------------GGCVLVA-------------------R 144
gi 13959540 173 LKTVTYSLLKRLKERs----LDTLLEAVESRgGVP------------------GGCVLVP-------------------R 211
gi 14194972  91 KQKWASRLLAKLRKDirpEYREDFVLTVTg--KKP-------------------PCCVLSn------------------- 130
gi 74819493  30 AEKSIRFLVNKKKPKl----TEDVEAAVM-QqTSK------------------TKCIVIYwsdwknhvrskldiggingS 86
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3QSV_A       69 TLDGRLQV-----------AGRKGFPHVIYA-----RLWRWPDL-HKNELKHVKYCQYA-FDLKCDS------VCVNPYH 124
gi 74965558  77 KLIGNFG-------------------RAIMCv---lRAFRFPVIrYESQVKSILTCRHA-FNSHSRN------VCLNPYH 127
gi 74763404 274 ------------------kTQTVWEPHVTAS-----RLFFWRELwNAKELKRLPTCPAArDCIy---------MCCNPLH 321
gi 82177797  78 ---------------------HGPPPHLLLC-----RLFRWPELqHPGQLKALSGCQGA-GGSDNNSg----cCCCNPYH 126
gi 82121089  99 KLDSRLG------------QQAFSLP-LLLC-----KVFRWPDLrHSSDVKRLSCCDSY-GKNNPEL------LCCNPHH 153
gi 13959538 131 RLDCRLGPgapagaqpaqpPSSYSLP-LLLC-----KVFRWPDLrHSSEVKRLCCCESY-GKINPEL------VCCNPHH 197
gi 13959573 145 G-ELRLG------------AARRPPPHLLLG-----KLFRWPDLqHPAELKALCECQSF-GAADGPT------VCCNPYH 199
gi 13959540 212 A-DLRLG-------------GQPAPPQLLLG-----RLFRWPDLqHAVELKPLCGCHSFtAAADGPT------VCCNPYH 266
gi 14194972 131 -------------------PDQKGKMRRIDClrqadKVWRL-DLvMVILFKGIPLESTDgERLVKSPqcsnpgLCVQPHH 190
gi 74819493  87 YNNGSTDI-----------ASHKLHPHVWFC-----RLWRWPDLtSQVELRAIPSCECP-FDQDKDQ------ICVQPYH 143

                ....*
3QSV_A      125 YERVV 129
gi 74965558 128 YRWVE 132
gi 74763404 322 WFRIL 326
gi 82177797 127 YSRVC 131
gi 82121089 154 LSRLC 158
gi 13959538 198 LSRLC 202
gi 13959573 200 FSRLC 204
gi 13959540 267 FSRLC 271
gi 14194972 191 IGVSV 195
gi 74819493 144 YSRVE 148
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